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Usp35 (NM_001177412) Mouse Tagged ORF Clone
CAT＃: MG219575
- TrueORF®
Usp35 (tGFP-tagged) - Mouse ubiquitin specific peptidase 35 (Usp35), (10ug). Note: ORF is codon optimized
ORF Plasmid: DDK
"NM_001177412" in other vectors (4)
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CNY 9,220.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Gm493; Gm1088 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG219575 representing NM_001177412, codon optimized.
Due to the complexity of NM_001177412, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATAAAATACTGGAAGCAGTGGTGACTAGCAGTTACCCTGCCAGTGTGAAGCAGGGACTGGTGAGAA GAGTGCTGGAGGCCGCCAGACAGCCCCTTGAGCGCGAACAGTGTTTGGCACTCCTCGCTCTGGGAGCTAG GCTGTACGTGGGGGGTACCGAAGAACTCCCCAGGAGAGTGGGCTGCCAGCTGCTCCATGTTGCCGGCCGG CATCATCCCGACGTGTTTGCCGAGTTCTTCAGCGCTAGGCGCGTCCTGCGCCTTCTTCAGGGGGGCGCTG GACCTCCTGGTGCGAGAGCACTGGCCTGCGTTCAGCTCGGGTTGCAACTCTTGCCTGATGGCCCTGCCGC GGACGAGGTCTTCGCCCTGCTCCGCAGGGAGGTACTGAGAACTGTTTGTGAACGGCCGGGCCCCGCTGCT TGTGCTCAGGTGGCCCGCCTGTTGGCTAGGCACCCACGCTGTGTGCCAGACGGTGCCCACAGGCTGCTCT TCTGCCAACAGCTTGTGAGATGCCTGGGCAGATTTAGGTGTCCTGCCGAAGGTGAGGAGGGCGCAGTAGA GTTCCTGGAGCAGGCCCAACAGGTGTCCGGGCTGCTCGCGCAGTTGTGGAGAGCACAGCCCGCCGCCATC CTCCCTTGTCTGAAAGAGCTTTTTGCCGTGATCTCCTGTACCGAGGAAGAGCCACCCTCTAGTGCCCTCG CCTCAGTGGTACAGCATCTTCCCCTTGAATTGATGGATGGTGTGGTCCGGAACCTCAGCAATGATGATAG TGTGACAGACTCCCAGATGCTGACTGCCATTAGCAGGATGATCGACTGGGTGTCCTGGCCCCTGGGGAAG AACATTGACAAGTGGATCATTGCCCTGCTGAAGGGCCTGGCTGCTGTTAAGAAGTTCAGCATCTTGATCG AGGTTTCGCTCGCCAAAATTGAGAAGGTCTTCTCTAAGCTGCTGTACCCCATCGTGCGGGGAGCTGCCCT ATCTGTCCTTAAGTACATGCTCCTGACTTTCCAGCACTCCCATGAGGCCTTCCACCTGCTTCTCCCTCAC ATTCCCGCCATGGTGGCCTCTCTGGTCAAGGAGGACTCGAACTCTGGGACCAGCTGCCTGGAGCAGCTGG CAGAGCTGGTGCACTGCATGGTGTTTCGGTTCCCGGGCTTCCCAGACCTGTATGAGCCTGTCATGGAAGC CATCAAGGACCTCCACATTCCCAATGAGGACCGAATCAAGCAGCTGCTGGGGCAGGATGCCTGGACCTCA CAGAAGAGTGAACTGGCTGGCTTCTATCCCCGGCTCATGGCCAAGTCAGACACGGGCAAGATTGGCTTAA TTAACTTGGGCAACACATGCTACGTGAACAGTGTCCTTCAGGCCTTATTCATGGCTTCTGACTTTCGACA CTGTGTGCTCCGCTTGACTGAGAACAACTCCCAGCCCTTGATGACCAAGCTGCAGTGGCTCTTTGCCTTC CTGGAGCACAGTCAGAGGCCTGCCATCTCTCCTGAGAACTTCCTCTCTGCGTCCTGGACGCCCTGGTTCA GCCCTGGCACCCAGCAAGACTGTTCAGAGTATCTGAAGTACCTGCTGGATCGGCTTCATGAAGAAGAGAA AACTGGGATGAGGATCTGCCAGAAGCTCAAGCAGTCCAGCTTGCCATCCCCACAGGAAGAGCTCCCCAGC TCCAATGCAACGTCGGTGGAGAAGATGTTCGGAGGCAAGATTGTGACCCGGATATGCTGTCTCCACTGCC TCAATGTTTCCTCAAGGGAGGAAGCCTTCACAGACCTCTCTTTGGCCTTCCCTCCTCCTGAGAGAAGTCG CCACCGCCGCCTCGGCTCCGTGATGCTCCCCACGGAGGATGTGCGAGTCCAGGAGTTGACACTGGCTCCC AGAGCCCCCGGGGCGCAGAGGCAGAGGAAGCGCTGCATCACAGGGGACGCTCCTCGCACTGGGCTGGACA GTGAAGGTGTGGACACCATAGGCACTGGTGGACGGAGTGGGCAGGAGAAGGTGGAGAGGGAGCAGGCTGG GAAGGAGAAGGAGGTGGCAGAGGACAGGGAAGAGGAAGGAACAAGGGAAGAGGAGAAAGAAGAGGGGGAA GAGAAAGACAAAGAGAAAGAGAAGAAGGAAGATGAAAAGGAAAAGGAAGCTGAGAATGGCAAGGAGAAGG AAGGGGACAGCTTAGGACCAGGGACCCATAGGGAGGCTGCCACTCCACCCAGGGAGCAGACATGCGGCCC TGAGGGTTCCCGCTCTGTACTGGACTTGGTCAACTACTTCCTGTCCCCTGAGAGGCTGACAGCTGAGAAC CGCTACTACTGTGAGTCCTGTGCCTCCCTGCAGGATGCGGAGAAGGTGGTGGAGCTGAGCCAGGGTCCAC GCTACCTCATCCTCACACTACTGCGCTTCTCCTTTGACCTGCGTACCATGCGGCGGCGTAAGATCCTGGA CGATGTCACCATCCCCCTTTTGCTGCGCCTGCCACTGGCTGGGGGTCAGGGCCAGGCCTATGACCTCTGT AGTGTGGTGGTGCACTCTGGAGTGTCCTCAGAGAGTGGCCACTATTACTGCTATGCTCGTGAGGGTGCTG CTCGGCCAGCTCCTGTCCTGGGATCCACAGAGAGGCCTGAGCCTGAGAACCAGTGGTACCTGTTTAATGA TACCCGGGTGTCTTACTCATCCTTTGAGTCTGTCAGCAATGTCACCTCCTTCTTCCCTAAGGACACTGCC TATGTGCTCTTCTACCGCCAGAGGCCCAGGGAGGACCCTGCGGCTGAGCCCGGCTCTCCTAGAGTCCGAG CAGAGCCTACCCTCCACAAGGACCTGATGGAGGCCATTTCCAAAGACAATGTCCTCTACCTGCAGGAGCA GGAGAAGGAGGCCCGGAGCAGGGCAGCTTATATCTCTACACTCCCTGCCCCTCCACACTGGGGTAGGGGC TTCGATGAAGACAAAGATGAGGATGAAGGCTCACCAGGGGGCTGCAACCCTGCAGGTGGCAATGGTGACT TCCACAGACTGGTCTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG219575 representing NM_001177412
Red=Cloning site Green=Tags(s) MDKILEAVVTSSYPASVKQGLVRRVLEAARQPLEREQCLALLALGARLYVGGTEELPRRVGCQLLHVAGR HHPDVFAEFFSARRVLRLLQGGAGPPGARALACVQLGLQLLPDGPAADEVFALLRREVLRTVCERPGPAA CAQVARLLARHPRCVPDGAHRLLFCQQLVRCLGRFRCPAEGEEGAVEFLEQAQQVSGLLAQLWRAQPAAI LPCLKELFAVISCTEEEPPSSALASVVQHLPLELMDGVVRNLSNDDSVTDSQMLTAISRMIDWVSWPLGK NIDKWIIALLKGLAAVKKFSILIEVSLAKIEKVFSKLLYPIVRGAALSVLKYMLLTFQHSHEAFHLLLPH IPAMVASLVKEDSNSGTSCLEQLAELVHCMVFRFPGFPDLYEPVMEAIKDLHIPNEDRIKQLLGQDAWTS QKSELAGFYPRLMAKSDTGKIGLINLGNTCYVNSVLQALFMASDFRHCVLRLTENNSQPLMTKLQWLFAF LEHSQRPAISPENFLSASWTPWFSPGTQQDCSEYLKYLLDRLHEEEKTGMRICQKLKQSSLPSPQEELPS SNATSVEKMFGGKIVTRICCLHCLNVSSREEAFTDLSLAFPPPERSRHRRLGSVMLPTEDVRVQELTLAP RAPGAQRQRKRCITGDAPRTGLDSEGVDTIGTGGRSGQEKVEREQAGKEKEVAEDREEEGTREEEKEEGE EKDKEKEKKEDEKEKEAENGKEKEGDSLGPGTHREAATPPREQTCGPEGSRSVLDLVNYFLSPERLTAEN RYYCESCASLQDAEKVVELSQGPRYLILTLLRFSFDLRTMRRRKILDDVTIPLLLRLPLAGGQGQAYDLC SVVVHSGVSSESGHYYCYAREGAARPAPVLGSTERPEPENQWYLFNDTRVSYSSFESVSNVTSFFPKDTA YVLFYRQRPREDPAAEPGSPRVRAEPTLHKDLMEAISKDNVLYLQEQEKEARSRAAYISTLPAPPHWGRG FDEDKDEDEGSPGGCNPAGGNGDFHRLVF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001177412 |
| ORF Size | 3027 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001177412.1, NP_001170883.1 |
| RefSeq Size | 3982 bp |
| RefSeq ORF | 3030 bp |
| Locus ID | 244144 |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223191 | Usp35 (untagged) - Mouse ubiquitin specific peptidase 35 (Usp35), (10ug) |
CNY 13,020.00 |
|
| MR219575 | Usp35 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 35 (Usp35). Note: ORF is codon optimized |
CNY 7,024.00 |
|
| MR219575L3 | Lenti ORF clone of Usp35 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 35 (Usp35). Note: ORF is codon optimized |
CNY 10,260.00 |
|
| MR219575L4 | Lenti ORF clone of Usp35 (mGFP-tagged) - Mouse ubiquitin specific peptidase 35 (Usp35). Note: ORF is codon optimized |
CNY 10,260.00 |
