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Sik1 (NM_010831) Mouse Tagged ORF Clone
CAT＃: MG219495
- TrueORF®
Sik1 (tGFP-tagged) - Mouse salt inducible kinase 1 (Sik1), (10ug)
ORF Plasmid: DDK
"NM_010831" in other vectors (4)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Hrt-20; Msk; Sik; Sik-1; Snf1lk |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG219495 representing NM_010831
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGATCATGTCGGAGTTCAGTGCGGTCCCCTCAGGCACTGGCCAGGGCCAGCAGAAGCCCCTCCGGG TGGGCTTTTACGACGTGGAACGGACCCTGGGCAAAGGCAATTTTGCAGTGGTTAAGCTGGCGCGGCACCG AGTCACCAAAACGCAGGTTGCAATAAAAATAATTGACAAGACACGGTTAGATTCTAGCAATCTGGAGAAG ATCTACCGGGAGGTCCAGCTCATGAAACTTTTGAACCACCCAAATATCATCAAGCTTTATCAGGTTATGG AGACAAAGGATATGCTCTACATTGTCACAGAATTTGCAAAAAATGGAGAAATGTTTGATTATCTGACTTC CAACGGGCACTTGAGTGAAAACGAGGCTAGGCAGAAGTTCTGGCAGATTCTGTCAGCCGTGGAGTACTGC CACAACCACCACATTGTCCACCGGGACCTCAAGACGGAGAACCTGCTCCTGGACAGCAACATGGATATCA AGCTAGCAGATTTTGGATTTGGGAATTTCTACAAGCCAGGCGAGCCTCTGTCCACGTGGTGTGGGAGCCC CCCGTATGCAGCCCCTGAAGTCTTCGAGGGGAAGGAGTACGAGGGTCCCCAGCTGGACGTCTGGAGCCTC GGTGTGGTGCTGTACGTCCTGGTCTGTGGCTCCCTCCCCTTCGACGGGCCCAACCTGCCTACGCTGAGAC AGCGGGTACTGGAGGGCCGCTTCCGCATCCCCTTCTTCATGTCTCAAGACTGTGAGACGCTGATCAGACG CATGCTGGTCGTGGACCCCGCTAAGCGCATCACCATAGCCCAGATCCGCCAGCACCGGTGGATGCAGGCC GACCCCACTCTCCTGCAGCAGGATGACCCTGCCTTCGACATGCAAGGCTACACCTCCAACCTGGGCGACT ACAACGAACAGGTGCTAGGGATCATGCAGGCCCTCGGCATCGACCGGCAGCGGACTATAGAGTCTCTGCA GAACAGCAGCTACAACCACTTTGCCGCCATTTACTACCTCCTACTCGAGCGCCTCAAGGAGCATCGAAGC GCCCAGCCCTCATCCCGGCCCACCCCTGCACCCACCAGACAGCCCCAGCTCCGAAGCTCAGACCTCAGCA GTCTGGAGGTTCCTCAAGAAATTCTCCCGTGTGACCCTTTCCGGCCCTCTCTGCTGTGCCCACAGCCCCA GGCCTTGGCTCAGTCTGTCCTGCAGGCTGAGATAGACTGTGATCTCCACAGCTCACTTCAGCCCTTATTA TTCCCCCTGGATACCAACTGCAGTGGAGTGTTCCGGCACCGATCCATCTCCCCCAGCAGTCTGCTGGACA CAGCTATCAGCGAGGAGGCCAGGCAGGGTCCCAGCCTAGAGGAGGAACAGGAGGTCCAGGAACCCCTGCC CGGAAGCACAGGCCGGAGGCACACATTGGCTGAAGTCTCCACCCATTTCTCCCCGCTCAACCCTCCTTGC ATAATTGTCTCCTCCTCTGCCACGGCGAGTCCCTCGGAAGGGACCAGCTCCGACAGCTGCCTCCCCTTCT CTGCAAGTGAAGGTCCTGCAGGGCTTGGCAGTGGCCTGGCCACCCCAGGGCTGCTGGGCACCAGCTCTCC AGTCAGATTGGCCTCGCCCTTCCTGGGATCACAGTCAGCCACCCCTGTGCTCCAGACTCAGGCAGGTCTG GGCACAGCCGTCTTACCTCCCGTCAGCTTCCAGGAGGGACGGAGAGCGTCTGATACCTCTCTCACTCAGG GGCTGAAGGCCTTCCGGCAGCAGCTGAGGAAAAATGCGAGGACCAAGGGGTTCCTGGGACTGAACAAGAT CAAAGGGTTGGCTCGCCAGGTGTGCCAGTCCTCCGTCCGAACTCCCCGGGGAGGGATGAGTACCTTCCAC ACCCCAGCCCCAAGCTCCGGCCTGCAAGGCTGCACGACCAGCAATCGGGAGGGCAGGAGCCTACTTGAAG AGGTCCTGCACCAGCAGAGGCTGCTCCAGTTACAGCACCACTCTTCTACCGCCGCCGCCTCATCTGGCTG CCAGCAGGGCCCCCAGCTGTCCCCGGTCCCCTATGTGCTTGCTCCCTGTGACAGCCTCCTCGTGTCTGGA ATTCCACTGCTGCCAACACCCCTTCTCCAGGCTGGCATGTCCCCTGTGGCGTCTGCTGCGCATCTCCTGG ATACCCACTTGCACATCAGTGCTGGCCCAGTGGCCCTCCCCACTGGGCCCCTGCCACAGTGCCTCACCAG GCTGTCCCCAGGCTGTGATCCAGCTGGGCTGCCACAGGGGGACTGTGAGATGGAGGACCTGACCTCTGGC CAGCGGGGGACATTCGTCCTGGTACAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG219495 representing NM_010831
Red=Cloning site Green=Tags(s) MVIMSEFSAVPSGTGQGQQKPLRVGFYDVERTLGKGNFAVVKLARHRVTKTQVAIKIIDKTRLDSSNLEK IYREVQLMKLLNHPNIIKLYQVMETKDMLYIVTEFAKNGEMFDYLTSNGHLSENEARQKFWQILSAVEYC HNHHIVHRDLKTENLLLDSNMDIKLADFGFGNFYKPGEPLSTWCGSPPYAAPEVFEGKEYEGPQLDVWSL GVVLYVLVCGSLPFDGPNLPTLRQRVLEGRFRIPFFMSQDCETLIRRMLVVDPAKRITIAQIRQHRWMQA DPTLLQQDDPAFDMQGYTSNLGDYNEQVLGIMQALGIDRQRTIESLQNSSYNHFAAIYYLLLERLKEHRS AQPSSRPTPAPTRQPQLRSSDLSSLEVPQEILPCDPFRPSLLCPQPQALAQSVLQAEIDCDLHSSLQPLL FPLDTNCSGVFRHRSISPSSLLDTAISEEARQGPSLEEEQEVQEPLPGSTGRRHTLAEVSTHFSPLNPPC IIVSSSATASPSEGTSSDSCLPFSASEGPAGLGSGLATPGLLGTSSPVRLASPFLGSQSATPVLQTQAGL GTAVLPPVSFQEGRRASDTSLTQGLKAFRQQLRKNARTKGFLGLNKIKGLARQVCQSSVRTPRGGMSTFH TPAPSSGLQGCTTSNREGRSLLEEVLHQQRLLQLQHHSSTAAASSGCQQGPQLSPVPYVLAPCDSLLVSG IPLLPTPLLQAGMSPVASAAHLLDTHLHISAGPVALPTGPLPQCLTRLSPGCDPAGLPQGDCEMEDLTSG QRGTFVLVQ TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010831 |
| ORF Size | 2337 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010831.3 |
| RefSeq Size | 4512 bp |
| RefSeq ORF | 2340 bp |
| Locus ID | 17691 |
| UniProt ID | Q60670 |
| Gene Summary | Serine/threonine-protein kinase involved in various processes such as cell cycle regulation, gluconeogenesis and lipogenesis regulation, muscle growth and differentiation and tumor suppression. Phosphorylates HDAC4, HDAC5, PPME1, SREBF1, CRTC1/TORC1 and CRTC2/TORC2. Acts as a tumor suppressor and plays a key role in p53/TP53-dependent anoikis, a type of apoptosis triggered by cell detachment: required for phosphorylation of p53/TP53 in response to loss of adhesion and is able to suppress metastasis. Part of a sodium-sensing signaling network, probably by mediating phosphorylation of PPME1: following increases in intracellular sodium, SIK1 is activated by CaMK1 and phosphorylates PPME1 subunit of protein phosphatase 2A (PP2A), leading to dephosphorylation of sodium/potassium-transporting ATPase ATP1A1 and subsequent increase activity of ATP1A1. Acts as a regulator of muscle cells by phosphorylating and inhibiting class II histone deacetylases HDAC4 and HDAC5, leading to promote expression of MEF2 target genes in myocytes. Also required during cardiomyogenesis by regulating the exit of cardiomyoblasts from the cell cycle via down-regulation of CDKN1C/p57Kip2. Acts as a regulator of hepatic gluconeogenesis by phosphorylating and repressing the CREB-specific coactivators CRTC1/TORC1 and CRTC2/TORC2, leading to inhibit CREB activity. Also regulates hepatic lipogenesis by phosphorylating and inhibiting SREBF1. In concert with CRTC1/TORC1, regulates the light-induced entrainment of the circadian clock by attenuating PER1 induction; represses CREB-mediated transcription of PER1 by phosphorylating and deactivating CRTC1/TORC1.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221558 | Sik1 (untagged) - Mouse salt inducible kinase 1 (Sik1), (10ug) |
CNY 6,288.00 |
|
| MR219495 | Sik1 (Myc-DDK-tagged) - Mouse salt inducible kinase 1 (Sik1) |
CNY 5,704.00 |
|
| MR219495L3 | Lenti ORF clone of Sik1 (Myc-DDK-tagged) - Mouse salt inducible kinase 1 (Sik1) |
CNY 7,700.00 |
|
| MR219495L4 | Lenti ORF clone of Sik1 (mGFP-tagged) - Mouse salt inducible kinase 1 (Sik1) |
CNY 7,700.00 |
