Il11 (NM_008350) Mouse Tagged ORF Clone
CAT#: MG219466
- TrueORF®
Il11 (tGFP-tagged) - Mouse interleukin 11 (Il11), (10ug)
ORF Plasmid: DDK
"NM_008350" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 4,000.00
CNY 1,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | IL-11 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG219466 representing NM_008350
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTGTGTTTGTCGCCTGGTCCTGGTGGTGCTGAGCCTCTGGCCAGATAGAGTCGTTGCCCCTGGGC CACCAGCTGGCTCCCCTCGAGTCTCTTCAGACCCTCGAGCAGATCTGGACAGCGCTGTTCTCCTAACCCG ATCCCTCCTGGCAGACACACGGCAACTAGCTGCACAGATGAGAGACAAATTCCCAGCTGACGGAGATCAC AGTCTGGACTCCCTGCCCACCTTGGCCATGAGCGCTGGGACATTGGGATCTTTGCAGCTTCCTGGTGTGC TGACAAGGCTTCGAGTAGACTTGATGTCCTACCTCCGGCATGTACAATGGCTGCGCCGTGCAGGTGGTCC TTCCCTAAAGACTCTGGAGCCAGAGCTGGGTGCCCTGCAAGCCCGACTGGAACGGCTACTCCGCCGTTTA CAGCTCTTGATGTCTCGCCTGGCCTTGCCCCAGGCAGCCCCAGACCAACCTGTGATCCCCCTGGGCCCTC CTGCCTCAGCCTGGGGAAGCATCCGGGCAGCTCATGCCATCCTAGGAGGGCTGCACCTGACCTTGGACTG GGCCGTGCGGGGCCTGCTGTTGTTAAAGACTCGACTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG219466 representing NM_008350
Red=Cloning site Green=Tags(s) MNCVCRLVLVVLSLWPDRVVAPGPPAGSPRVSSDPRADLDSAVLLTRSLLADTRQLAAQMRDKFPADGDH SLDSLPTLAMSAGTLGSLQLPGVLTRLRVDLMSYLRHVQWLRRAGGPSLKTLEPELGALQARLERLLRRL QLLMSRLALPQAAPDQPVIPLGPPASAWGSIRAAHAILGGLHLTLDWAVRGLLLLKTRL TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_008350 |
ORF Size | 597 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_008350.4, NP_032376.1 |
RefSeq Size | 1949 bp |
RefSeq ORF | 600 bp |
Locus ID | 16156 |
UniProt ID | P47873 |
Gene Summary | Cytokine that stimulates the proliferation of hematopoietic stem cells and megakaryocyte progenitor cells and induces megakaryocyte maturation resulting in increased platelet production (PubMed:8913282). Also promotes the proliferation of hepatocytes in response to liver damage (PubMed:22253262). Binding to its receptor formed by IL6ST and either IL11RA1 or IL11RA2 activates a signaling cascade that promotes cell proliferation, also in the context of various cancers (PubMed:10026196, PubMed:23948300). Signaling leads to the activation of intracellular protein kinases and the phosphorylation of STAT3 (PubMed:23948300, PubMed:22253262).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208766 | Il11 (untagged) - Mouse interleukin 11 (Il11), (10ug) |
CNY 2,400.00 |
|
MR219466 | Il11 (Myc-DDK-tagged) - Mouse interleukin 11 (Il11) |
CNY 2,400.00 |
|
MR219466L3 | Lenti ORF clone of Il11 (Myc-DDK-tagged) - Mouse interleukin 11 (Il11) |
CNY 4,750.00 |
|
MR219466L4 | Lenti ORF clone of Il11 (mGFP-tagged) - Mouse interleukin 11 (Il11) |
CNY 4,750.00 |