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Il10ra (NM_008348) Mouse Tagged ORF Clone
CAT＃: MG219426
- TrueORF®
Il10ra (tGFP-tagged) - Mouse interleukin 10 receptor alpha (Il10ra), (10ug)
ORF Plasmid: DDK
"NM_008348" in other vectors (4)
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CNY 5,510.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AW553859; CDw210; CDw210a; IL-10R1; IL-10RA; Il10r; mIL-10R |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG219426 representing NM_008348
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGTCGCGTTTGCTCCCATTCCTCGTCACGATCTCCAGCCTGAGCCTAGAATTCATTGCATACGGGA CAGAACTGCCAAGCCCTTCCTATGTGTGGTTTGAAGCCAGATTTTTCCAGCACATCCTCCACTGGAAACC TATCCCAAACCAGTCTGAGAGCACCTACTATGAAGTGGCCCTCAAACAGTACGGAAACTCAACCTGGAAT GACATCCATATCTGTAGAAAGGCTCAGGCATTGTCCTGTGATCTCACAACGTTCACCCTGGATCTGTATC ACCGAAGCTATGGCTACCGGGCCAGAGTCCGGGCAGTGGACAACAGTCAGTACTCCAACTGGACCACCAC TGAGACTCGCTTCACAGTGGATGAAGTGATTCTGACAGTGGATAGCGTGACTCTGAAAGCAATGGACGGC ATCATCTATGGGACAATCCATCCCCCCAGGCCCACGATAACCCCTGCAGGGGATGAGTACGAACAAGTCT TCAAGGATCTCCGAGTTTACAAGATTTCCATCCGGAAGTTCTCAGAACTAAAGAATGCAACCAAGAGAGT GAAACAGGAAACCTTCACCCTCACGGTCCCCATAGGGGTGAGAAAGTTTTGTGTCAAGGTGCTGCCCCGC TTGGAATCCCGAATTAACAAGGCAGAGTGGTCGGAGGAGCAGTGTTTACTTATCACGACGGAGCAGTATT TCACTGTGACCAACCTGAGCATCTTAGTCATATCTATGCTGCTATTCTGTGGAATCCTGGTCTGTCTGGT TCTCCAGTGGTACATCCGGCACCCGGGGAAGTTGCCTACAGTCCTGGTCTTCAAGAAGCCTCACGACTTC TTCCCAGCCAACCCTCTCTGCCCAGAAACTCCCGATGCCATTCACATCGTGGACCTGGAGGTTTTCCCAA AGGTGTCACTAGAGCTGAGAGACTCAGTCCTGCATGGCAGCACCGACAGTGGCTTTGGCAGTGGTAAACC ATCACTTCAGACTGAAGAGTCCCAATTCCTCCTCCCTGGCTCCCACCCCCAGATACAGGGGACTCTGGGA AAAGAAGAGTCTCCAGGGCTACAGGCCACCTGTGGGGACAACACGGACAGTGGGATCTGCCTGCAGGAGC CCGGCTTACACTCCAGCATGGGGCCCGCCTGGAAGCAGCAGCTTGGATATACCCATCAGGACCAGGATGA CAGTGACGTTAACCTAGTCCAGAACTCTCCAGGGCAGCCTAAGTACACACAGGATGCATCTGCCTTGGGC CATGTCTGTCTCCTAGAACCTAAAGCCCCTGAGGAGAAAGACCAAGTCATGGTGACATTCCAGGGCTACC AGAAACAGACCAGATGGAAGGCAGAGGCAGCAGGCCCAGCAGAATGCTTGGACGAAGAGATTCCCTTGAC AGATGCCTTTGATCCTGAACTTGGGGTACACCTGCAGGATGATTTGGCTTGGCCTCCACCAGCTCTGGCC GCAGGTTATTTGAAACAGGAGTCTCAAGGGATGGCTTCTGCTCCACCAGGGACACCAAGTAGACAGTGGA ATCAACTGACCGAAGAGTGGTCACTCCTGGGTGTGGTTAGCTGTGAAGATCTAAGCATAGAAAGTTGGAG GTTTGCCCATAAACTTGACCCTCTGGACTGTGGGGCAGCCCCTGGTGGCCTCCTGGATAGCCTTGGCTCT AACCTGGTCACCCTGCCGTTGATCTCCAGCCTGCAGGTAGAAGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG219426 representing NM_008348
Red=Cloning site Green=Tags(s) MLSRLLPFLVTISSLSLEFIAYGTELPSPSYVWFEARFFQHILHWKPIPNQSESTYYEVALKQYGNSTWN DIHICRKAQALSCDLTTFTLDLYHRSYGYRARVRAVDNSQYSNWTTTETRFTVDEVILTVDSVTLKAMDG IIYGTIHPPRPTITPAGDEYEQVFKDLRVYKISIRKFSELKNATKRVKQETFTLTVPIGVRKFCVKVLPR LESRINKAEWSEEQCLLITTEQYFTVTNLSILVISMLLFCGILVCLVLQWYIRHPGKLPTVLVFKKPHDF FPANPLCPETPDAIHIVDLEVFPKVSLELRDSVLHGSTDSGFGSGKPSLQTEESQFLLPGSHPQIQGTLG KEESPGLQATCGDNTDSGICLQEPGLHSSMGPAWKQQLGYTHQDQDDSDVNLVQNSPGQPKYTQDASALG HVCLLEPKAPEEKDQVMVTFQGYQKQTRWKAEAAGPAECLDEEIPLTDAFDPELGVHLQDDLAWPPPALA AGYLKQESQGMASAPPGTPSRQWNQLTEEWSLLGVVSCEDLSIESWRFAHKLDPLDCGAAPGGLLDSLGS NLVTLPLISSLQVEE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008348 |
| ORF Size | 1725 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008348.3 |
| RefSeq Size | 3493 bp |
| RefSeq ORF | 1728 bp |
| Locus ID | 16154 |
| UniProt ID | Q61727 |
| Gene Summary | Cell surface receptor for the cytokine IL10 that participates in IL10-mediated anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Upon binding to IL10, induces a conformational change in IL10RB, allowing IL10RB to bind IL10 as well. In turn, the heterotetrameric assembly complex, composed of two subunits of IL10RA and IL10RB, activates the kinases JAK1 and TYK2 that are constitutively associated with IL10RA and IL10RB respectively. These kinases then phosphorylate specific tyrosine residues in the intracellular domain in IL10RA leading to the recruitment and subsequent phosphorylation of STAT3 (PubMed:8910398). Once phosphorylated, STAT3 homodimerizes, translocates to the nucleus and activates the expression of anti-inflammatory genes. In addition, IL10RA-mediated activation of STAT3 inhibits starvation-induced autophagy (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219161 | Il10ra (untagged) - Mouse interleukin 10 receptor, alpha (Il10ra), (10ug) |
CNY 4,288.00 |
|
| MR219426 | Il10ra (Myc-DDK-tagged) - Mouse interleukin 10 receptor, alpha (Il10ra) |
CNY 4,280.00 |
|
| MR219426L3 | Lenti ORF clone of Il10ra (Myc-DDK-tagged) - Mouse interleukin 10 receptor, alpha (Il10ra) |
CNY 6,940.00 |
|
| MR219426L4 | Lenti ORF clone of Il10ra (mGFP-tagged) - Mouse interleukin 10 receptor, alpha (Il10ra) |
CNY 6,940.00 |
