Pigx (NM_001111025) Mouse Tagged ORF Clone
CAT#: MG218595
- TrueORF®
Pigx (tGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis class X (Pigx) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_001111025" in other vectors (3)
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CNY 2,760.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 2010319C14Rik; PIG-X |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG218595 representing NM_001111025
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC CTGGCTGCCGGCGCTGTGGCCTGGCTGCTCCTTTGGGCAGCCTGGCTGGTCGGCAGGCTCGCCGCGGACT TCAGCGACGCCCCTTTCAGCGCTGGTGTAAGGGCCACTTGTTCTGAAATTATTTTAAGGCAAGAATTTTT GAAAGATGGTTTCCACAGAGACCTTTTAATAAAAGTGAAATTTGGAGAGAGCATTGAGGACCTACAGACC TGCCGGCTCCTAATTAAGCATTACATTCCACCAGGACTCTTTGTGGATCCCTATGAGTTGGCTTCACTAC GAGAAAGGAACTTAACAGAGGCAGTGATGCTATCAGAAAGTTTTAATATAGAAGCCCCCAACTACTTGTC CAATGAATCTGCAGTCCTCATTTATGCCCGGCAGGATGCACAGTGCATCGACTGCTTCCAGGCCTTTTTA CCTGTGCACTATCGCTATCACCGGCCACATAAGAAAGATGGAGACACCCTCATTGTGGTCAACAACCCCG ACTTACTGATGTACTGTGACCAAGAGTTTCCAATTTTGAAATGCTGGGCTCAGTCAGAAGTAGCAGCTCC TTGTGCTTTGAAGAGTGAGGAGATCTGCCAGTGGAAAAGCATGCAGTACAAATCAATACTTAAGAATTTG ACAGTGCAGGTTCCAGTGGGACTGACTATACATACCTCTTTAGTGTGTTCTGTGACGCTGCTCATTACGA TTCTGTGTTCTACTTTGATCCTTTTAGCTGTTTTCAAATATGGCCACTTTTCCCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218595 representing NM_001111025
Red=Cloning site Green=Tags(s) LAAGAVAWLLLWAAWLVGRLAADFSDAPFSAGVRATCSEIILRQEFLKDGFHRDLLIKVKFGESIEDLQT CRLLIKHYIPPGLFVDPYELASLRERNLTEAVMLSESFNIEAPNYLSNESAVLIYARQDAQCIDCFQAFL PVHYRYHRPHKKDGDTLIVVNNPDLLMYCDQEFPILKCWAQSEVAAPCALKSEEICQWKSMQYKSILKNL TVQVPVGLTIHTSLVCSVTLLITILCSTLILLAVFKYGHFSL TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001111025 |
ORF Size | 756 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001111025.1, NP_001104495.1 |
RefSeq Size | 1013 bp |
RefSeq ORF | 759 bp |
Locus ID | 72084 |
Gene Summary | This gene encodes a type I transmembrane protein in the endoplasmic reticulum (ER). The protein is an essential component of glycosylphosphatidylinositol-mannosyltransferase I, which transfers the first of the four mannoses in the GPI-anchor precursors during GPI-anchor biosynthesis. Studies in rat indicate that the protein is translated from a non-AUG translation initiation site. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR218595 | Pigx (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class X (Pigx), transcript variant 2 |
CNY 2,470.00 |
|
MR218595L3 | Lenti ORF clone of Pigx (Myc-DDK-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class X (Pigx), transcript variant 2 |
CNY 4,370.00 |
|
MR218595L4 | Lenti ORF clone of Pigx (mGFP-tagged) - Mouse phosphatidylinositol glycan anchor biosynthesis, class X (Pigx), transcript variant 2 |
CNY 4,370.00 |