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Ndst4 (NM_022565) Mouse Tagged ORF Clone
CAT＃: MG218546
- TrueORF®
Ndst4 (tGFP-tagged) - Mouse N-deacetylase/N-sulfotransferase (heparin glucosaminyl) 4 (Ndst4), (10ug)
ORF Plasmid: DDK
"NM_022565" in other vectors (4)
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CNY 8,460.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4930439H17Rik |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG218546 representing NM_022565
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCTTATTTTAAAGTTTCGAAGAAGCTTTCGAACACTGATTGTTCTCTTAGCTACTTTTTGCTTGG TGAGCATCCTCATTTCTGCCTACTTCCTGTACTCTGGCTACAAACAGGAAATGACACTTATTGAAACGAC TGCAGAAGCAGAGTGTGCTGACATCAAAGACCTCCCTTATCGGTCTATAGAGCTGAGAACCATCAAGCCT ATTGATACATCCAAAACTGACCCCACTGTCCTTCTCTTTGTAGAGAGCCAGTATTCGCAACTTGGTCAAG ATATTATAGCAATCTTGGAGTCCAGTCGGTTTCAATACCAGATGGTCATTGCTCCAGGCAAGGGAGACAT ACCGCCTCTTACCGATAGTGGCAAAGGGAAGTACACATTGATTATTTATGAAAATATTCTGAAGTACGTC AGCATGGACTCATGGAACCGGGAGCTTTTAGAAAAATACTGTATAGAATACAGTGTTAGCATAATTGGTT TTCATAAAGCCAATGAGAACAGTTTACCAACTACACAACTGAAAGGGTTTCCTTTGAACTTATTCAATAA CGTAGCTCTGAAGGACTGCTCTGTGAACCCCCAGTCTCCTCTGCTCCATATTACCAAAGGCCCCAAAGTT GAAAAGGGACCTCTTCCTGGGGAAGACTGGACAATTTTCCAGTATAACCATTCTACCTATCAGCCAGTGC TTTTAACTGAGTTACAGTCAGAAAAGTCCCTCTCTTTCTTGTCCAGCCAAACACTTTATGCGACAATTAT TCAGGATCTGGGGCTTCATGACGGGATTCAGCGAGTTCTTTTTGGTAACAACTTGAACTTTTGGTTGCAC AAGCTCATTTTCATAGACGCCATCTCCTTCTTGTCGGGGAAGAGGTTGACACTGTCCTTGGACAGGTACA TCCTTGTGGACATTGATGACATATTTGTGGGGAAGGAGGGCACGAGAATGAATGTCAAAGATGTGAAGGC ATTACTAGAGACTCAAAATTTACTGCGCACTCAGGTTGCAAATTTTACCTTCAACCTTGGATTTTCAGGG AAGTTTTACCACACAGGAACCGAAGAAGAGGATGAAGGTGACGACCTTCTGCTGAGGTCTGTGGATGAAT TCTGGTGGTTTCCTCACATGTGGAGTCACATGCAGCCCCACCTCTTCCACAATGAATCATCTTTAGTGGA ACAGATGATTCTCAACAAAGAATTTGCCCTGGAACATGGAATTCCGATCAACCTGGGCTACGCAGTGGCC CCACATCACTCAGGGGTCTACCCAGTCCATATTCAGTTGTATGCAGCTTGGAAGAAAGTCTGGGGTATTC AGGTCACCAGCACTGAAGAGTACCCACATCTGAAACCTGCGCGGTACAGGAAGGGCTTCATTCATAATAG CATCATGGTCCTCCCTCGACAGACCTGTGGGCTGTTCACACATACTATTTTCTACAAGGAGTATCCAGGA GGACCTCAAGAATTGGATAAAAGTATCAAAGGAGGTGAACTCTTCCTCACAATCCTTCTAAATCCAATCA GCATTTTCATGACACATCTTTCTAACTATGGAAATGACCGCCTCGGCTTGTACACCTTTGTGAACTTGGC CAACTTTGTGCACAGCTGGACCAACTTGAAACTGCAGACCCTGCCTCCAGTGCAACTGGCCCACAAATAC TTCGAGCTCTTCCCTGAGCAGAAAGACCCTCTCTGGCAGAATCCATGCGACGATAAACGACACAAAGACA TCTGGTCCAGGGAGAAAACCTGTGACCATTTACCGAAATTCCTTGTGATTGGGCCACAGAAGACAGGAAC AACTGCACTTTATTTATTTCTTCTAATGCACCCCTCCATCATCAGCAATCTTCCTAGCCCCAAAACCTTT GAAGAAGTTCAATTTTTTAATGGCAACAACTATCACAAGGGAATTGAGTGGTATATGGACTTTTTCCCCA CTCCTTCCAACATTACCAGTGATTTCCTATTTGAAAAGAGTGCTAACTACTTCCACTCAGAAGAGGCTCC CAAGAGGGCTGCATCTCTTGTTCCCAAAGCCAAGATCATTACTATCCTCATAGACCCCTCGGACAGGGCA TATTCTTGGTACCAGCACCAACGATCTCATGAAGATCCAGCTGCCCTGAGGTTCAATTTCTATGAAGTTA TCACAACAGGACATTGGGCCCCTCCTGATTTAAAAACATTGCAGAGGAGATGTCTGGTGCCTGGGTGGTA TGCAGTGCACATAGAAAGATGGCTGGCCTACTTTTCTACTTCCCAGTTGCTAATTATTGATGGACAACAG CTGAGATCTGACCCAGCTACTGTGATGGATGAAGTCCAGAAGTTTCTAGGAGTTACACCTCATTATAATT ACTCGGAAGCACTAACGTTTGATCCCCAGAAAGGCTTTTGGTGTCAGCTACTAGAAGGAGGAAAAACCAA ATGCCTTGGGAAAAGCAAAGGCCGAAAATACCCGCCCATGGATTCAGAGTCCAGAACTTTCCTCTCCAGC TACTACCGCGACCACAACGTGGAACTCTCGAAGCTGCTGCACAGGCTGGGGCAGCCTCTGCCCTCGTGGC TGAGACAGGAGCTGCAGAAAGTGAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218546 representing NM_022565
Red=Cloning site Green=Tags(s) MNLILKFRRSFRTLIVLLATFCLVSILISAYFLYSGYKQEMTLIETTAEAECADIKDLPYRSIELRTIKP IDTSKTDPTVLLFVESQYSQLGQDIIAILESSRFQYQMVIAPGKGDIPPLTDSGKGKYTLIIYENILKYV SMDSWNRELLEKYCIEYSVSIIGFHKANENSLPTTQLKGFPLNLFNNVALKDCSVNPQSPLLHITKGPKV EKGPLPGEDWTIFQYNHSTYQPVLLTELQSEKSLSFLSSQTLYATIIQDLGLHDGIQRVLFGNNLNFWLH KLIFIDAISFLSGKRLTLSLDRYILVDIDDIFVGKEGTRMNVKDVKALLETQNLLRTQVANFTFNLGFSG KFYHTGTEEEDEGDDLLLRSVDEFWWFPHMWSHMQPHLFHNESSLVEQMILNKEFALEHGIPINLGYAVA PHHSGVYPVHIQLYAAWKKVWGIQVTSTEEYPHLKPARYRKGFIHNSIMVLPRQTCGLFTHTIFYKEYPG GPQELDKSIKGGELFLTILLNPISIFMTHLSNYGNDRLGLYTFVNLANFVHSWTNLKLQTLPPVQLAHKY FELFPEQKDPLWQNPCDDKRHKDIWSREKTCDHLPKFLVIGPQKTGTTALYLFLLMHPSIISNLPSPKTF EEVQFFNGNNYHKGIEWYMDFFPTPSNITSDFLFEKSANYFHSEEAPKRAASLVPKAKIITILIDPSDRA YSWYQHQRSHEDPAALRFNFYEVITTGHWAPPDLKTLQRRCLVPGWYAVHIERWLAYFSTSQLLIIDGQQ LRSDPATVMDEVQKFLGVTPHYNYSEALTFDPQKGFWCQLLEGGKTKCLGKSKGRKYPPMDSESRTFLSS YYRDHNVELSKLLHRLGQPLPSWLRQELQKVR TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022565 |
| ORF Size | 2616 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022565.2, NP_072087.2 |
| RefSeq Size | 3489 bp |
| RefSeq ORF | 2619 bp |
| Locus ID | 64580 |
| UniProt ID | Q9EQW8 |
| Gene Summary | Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Has low deacetylase activity but high sulfotransferase activity.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222328 | Ndst4 (untagged) - Mouse N-deacetylase/N-sulfotransferase (heparin glucosaminyl) 4 (Ndst4), (10ug) |
CNY 6,392.00 |
|
| MR218546 | Ndst4 (Myc-DDK-tagged) - Mouse N-deacetylase/N-sulfotransferase (heparin glucosaminyl) 4 (Ndst4) |
CNY 6,384.00 |
|
| MR218546L3 | Lenti ORF clone of Ndst4 (Myc-DDK-tagged) - Mouse N-deacetylase/N-sulfotransferase (heparin glucosaminyl) 4 (Ndst4) |
CNY 9,600.00 |
|
| MR218546L4 | Lenti ORF clone of Ndst4 (mGFP-tagged) - Mouse N-deacetylase/N-sulfotransferase (heparin glucosaminyl) 4 (Ndst4) |
CNY 9,600.00 |
