Ndst4 (NM_022565) Mouse Untagged Clone

CAT#: MC222328

Ndst4 (untagged) - Mouse N-deacetylase/N-sulfotransferase (heparin glucosaminyl) 4 (Ndst4), (10ug)



  "NM_022565" in other vectors (4)

CNY 6,392.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 4930439H17Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC222328 representing NM_022565
Red=Cloning site Blue=ORF

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAATCTTATTTTAAAGTTTCGAAGAAGCTTTCGAACACTGATTGTTCTCTTAGCTACTTTTTGCTTGG
TGAGCATCCTCATTTCTGCCTACTTCCTGTACTCTGGCTACAAACAGGAAATGACACTTATTGAAACGAC
TGCAGAAGCAGAGTGTGCTGACATCAAAGACCTCCCTTATCGGTCTATAGAGCTGAGAACCATCAAGCCT
ATTGATACATCCAAAACTGACCCCACTGTCCTTCTCTTTGTAGAGAGCCAGTATTCGCAACTTGGTCAAG
ATATTATAGCAATCTTGGAGTCCAGTCGGTTTCAATACCAGATGGTCATTGCTCCAGGCAAGGGAGACAT
ACCGCCTCTTACCGATAGTGGCAAAGGGAAGTACACATTGATTATTTATGAAAATATTCTGAAGTACGTC
AGCATGGACTCATGGAACCGGGAGCTTTTAGAAAAATACTGTATAGAATACAGTGTTAGCATAATTGGTT
TTCATAAAGCCAATGAGAACAGTTTACCAACTACACAACTGAAAGGGTTTCCTTTGAACTTATTCAATAA
CGTAGCTCTGAAGGACTGCTCTGTGAACCCCCAGTCTCCTCTGCTCCATATTACCAAAGGCCCCAAAGTT
GAAAAGGGACCTCTTCCTGGGGAAGACTGGACAATTTTCCAGTATAACCATTCTACCTATCAGCCAGTGC
TTTTAACTGAGTTACAGTCAGAAAAGTCCCTCTCTTTCTTGTCCAGCCAAACACTTTATGCGACAATTAT
TCAGGATCTGGGGCTTCATGACGGGATTCAGCGAGTTCTTTTTGGTAACAACTTGAACTTTTGGTTGCAC
AAGCTCATTTTCATAGACGCCATCTCCTTCTTGTCGGGGAAGAGGTTGACACTGTCCTTGGACAGGTACA
TCCTTGTGGACATTGATGACATATTTGTGGGGAAGGAGGGCACGAGAATGAATGTCAAAGATGTGAAGGC
ATTACTAGAGACTCAAAATTTACTGCGCACTCAGGTTGCAAATTTTACCTTCAACCTTGGATTTTCAGGG
AAGTTTTACCACACAGGAACCGAAGAAGAGGATGAAGGTGACGACCTTCTGCTGAGGTCTGTGGATGAAT
TCTGGTGGTTTCCTCACATGTGGAGTCACATGCAGCCCCACCTCTTCCACAATGAATCATCTTTAGTGGA
ACAGATGATTCTCAACAAAGAATTTGCCCTGGAACATGGAATTCCGATCAACCTGGGCTACGCAGTGGCC
CCACATCACTCAGGGGTCTACCCAGTCCATATTCAGTTGTATGCAGCTTGGAAGAAAGTCTGGGGTATTC
AGGTCACCAGCACTGAAGAGTACCCACATCTGAAACCTGCGCGGTACAGGAAGGGCTTCATTCATAATAG
CATCATGGTCCTCCCTCGACAGACCTGTGGGCTGTTCACACATACTATTTTCTACAAGGAGTATCCAGGA
GGACCTCAAGAATTGGATAAAAGTATCAAAGGAGGTGAACTCTTCCTCACAATCCTTCTAAATCCAATCA
GCATTTTCATGACACATCTTTCTAACTATGGAAATGACCGCCTCGGCTTGTACACCTTTGTGAACTTGGC
CAACTTTGTGCACAGCTGGACCAACTTGAAACTGCAGACCCTGCCTCCAGTGCAACTGGCCCACAAATAC
TTCGAGCTCTTCCCTGAGCAGAAAGACCCTCTCTGGCAGAATCCATGCGACGATAAACGACACAAAGACA
TCTGGTCCAGGGAGAAAACCTGTGACCATTTACCGAAATTCCTTGTGATTGGGCCACAGAAGACAGGAAC
AACTGCACTTTATTTATTTCTTCTAATGCACCCCTCCATCATCAGCAATCTTCCTAGCCCCAAAACCTTT
GAAGAAGTTCAATTTTTTAATGGCAACAACTATCACAAGGGAATTGAGTGGTATATGGACTTTTTCCCCA
CTCCTTCCAACATTACCAGTGATTTCCTATTTGAAAAGAGTGCTAACTACTTCCACTCAGAAGAGGCTCC
CAAGAGGGCTGCATCTCTTGTTCCCAAAGCCAAGATCATTACTATCCTCATAGACCCCTCGGACAGGGCA
TATTCTTGGTACCAGCACCAACGATCTCATGAAGATCCAGCTGCCCTGAGGTTCAATTTCTATGAAGTTA
TCACAACAGGACATTGGGCCCCTCCTGATTTAAAAACATTGCAGAGGAGATGTCTGGTGCCTGGGTGGTA
TGCAGTGCACATAGAAAGATGGCTGGCCTACTTTTCTACTTCCCAGTTGCTAATTATTGATGGACAACAG
CTGAGATCTGACCCAGCTACTGTGATGGATGAAGTCCAGAAGTTTCTAGGAGTTACACCTCATTATAATT
ACTCGGAAGCACTAACGTTTGATCCCCAGAAAGGCTTTTGGTGTCAGCTACTAGAAGGAGGAAAAACCAA
ATGCCTTGGGAAAAGCAAAGGCCGAAAATACCCGCCCATGGATTCAGAGTCCAGAACTTTCCTCTCCAGC
TACTACCGCGACCACAACGTGGAACTCTCGAAGCTGCTGCACAGGCTGGGGCAGCCTCTGCCCTCGTGGC
TGAGACAGGAGCTGCAGAAAGTGAGGTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI     
ACCN NM_022565
Insert Size 2619 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_022565.2, NP_072087.2
RefSeq Size 3489 bp
RefSeq ORF 2619 bp
Locus ID 64580
UniProt ID Q9EQW8
Gene Summary Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Has low deacetylase activity but high sulfotransferase activity.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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