Ndst4 (NM_022565) Mouse Untagged Clone
CAT#: MC222328
Ndst4 (untagged) - Mouse N-deacetylase/N-sulfotransferase (heparin glucosaminyl) 4 (Ndst4), (10ug)
CNY 6,392.00
Product images
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | 4930439H17Rik |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC222328 representing NM_022565
Red=Cloning site Blue=ORF TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCTTATTTTAAAGTTTCGAAGAAGCTTTCGAACACTGATTGTTCTCTTAGCTACTTTTTGCTTGG TGAGCATCCTCATTTCTGCCTACTTCCTGTACTCTGGCTACAAACAGGAAATGACACTTATTGAAACGAC TGCAGAAGCAGAGTGTGCTGACATCAAAGACCTCCCTTATCGGTCTATAGAGCTGAGAACCATCAAGCCT ATTGATACATCCAAAACTGACCCCACTGTCCTTCTCTTTGTAGAGAGCCAGTATTCGCAACTTGGTCAAG ATATTATAGCAATCTTGGAGTCCAGTCGGTTTCAATACCAGATGGTCATTGCTCCAGGCAAGGGAGACAT ACCGCCTCTTACCGATAGTGGCAAAGGGAAGTACACATTGATTATTTATGAAAATATTCTGAAGTACGTC AGCATGGACTCATGGAACCGGGAGCTTTTAGAAAAATACTGTATAGAATACAGTGTTAGCATAATTGGTT TTCATAAAGCCAATGAGAACAGTTTACCAACTACACAACTGAAAGGGTTTCCTTTGAACTTATTCAATAA CGTAGCTCTGAAGGACTGCTCTGTGAACCCCCAGTCTCCTCTGCTCCATATTACCAAAGGCCCCAAAGTT GAAAAGGGACCTCTTCCTGGGGAAGACTGGACAATTTTCCAGTATAACCATTCTACCTATCAGCCAGTGC TTTTAACTGAGTTACAGTCAGAAAAGTCCCTCTCTTTCTTGTCCAGCCAAACACTTTATGCGACAATTAT TCAGGATCTGGGGCTTCATGACGGGATTCAGCGAGTTCTTTTTGGTAACAACTTGAACTTTTGGTTGCAC AAGCTCATTTTCATAGACGCCATCTCCTTCTTGTCGGGGAAGAGGTTGACACTGTCCTTGGACAGGTACA TCCTTGTGGACATTGATGACATATTTGTGGGGAAGGAGGGCACGAGAATGAATGTCAAAGATGTGAAGGC ATTACTAGAGACTCAAAATTTACTGCGCACTCAGGTTGCAAATTTTACCTTCAACCTTGGATTTTCAGGG AAGTTTTACCACACAGGAACCGAAGAAGAGGATGAAGGTGACGACCTTCTGCTGAGGTCTGTGGATGAAT TCTGGTGGTTTCCTCACATGTGGAGTCACATGCAGCCCCACCTCTTCCACAATGAATCATCTTTAGTGGA ACAGATGATTCTCAACAAAGAATTTGCCCTGGAACATGGAATTCCGATCAACCTGGGCTACGCAGTGGCC CCACATCACTCAGGGGTCTACCCAGTCCATATTCAGTTGTATGCAGCTTGGAAGAAAGTCTGGGGTATTC AGGTCACCAGCACTGAAGAGTACCCACATCTGAAACCTGCGCGGTACAGGAAGGGCTTCATTCATAATAG CATCATGGTCCTCCCTCGACAGACCTGTGGGCTGTTCACACATACTATTTTCTACAAGGAGTATCCAGGA GGACCTCAAGAATTGGATAAAAGTATCAAAGGAGGTGAACTCTTCCTCACAATCCTTCTAAATCCAATCA GCATTTTCATGACACATCTTTCTAACTATGGAAATGACCGCCTCGGCTTGTACACCTTTGTGAACTTGGC CAACTTTGTGCACAGCTGGACCAACTTGAAACTGCAGACCCTGCCTCCAGTGCAACTGGCCCACAAATAC TTCGAGCTCTTCCCTGAGCAGAAAGACCCTCTCTGGCAGAATCCATGCGACGATAAACGACACAAAGACA TCTGGTCCAGGGAGAAAACCTGTGACCATTTACCGAAATTCCTTGTGATTGGGCCACAGAAGACAGGAAC AACTGCACTTTATTTATTTCTTCTAATGCACCCCTCCATCATCAGCAATCTTCCTAGCCCCAAAACCTTT GAAGAAGTTCAATTTTTTAATGGCAACAACTATCACAAGGGAATTGAGTGGTATATGGACTTTTTCCCCA CTCCTTCCAACATTACCAGTGATTTCCTATTTGAAAAGAGTGCTAACTACTTCCACTCAGAAGAGGCTCC CAAGAGGGCTGCATCTCTTGTTCCCAAAGCCAAGATCATTACTATCCTCATAGACCCCTCGGACAGGGCA TATTCTTGGTACCAGCACCAACGATCTCATGAAGATCCAGCTGCCCTGAGGTTCAATTTCTATGAAGTTA TCACAACAGGACATTGGGCCCCTCCTGATTTAAAAACATTGCAGAGGAGATGTCTGGTGCCTGGGTGGTA TGCAGTGCACATAGAAAGATGGCTGGCCTACTTTTCTACTTCCCAGTTGCTAATTATTGATGGACAACAG CTGAGATCTGACCCAGCTACTGTGATGGATGAAGTCCAGAAGTTTCTAGGAGTTACACCTCATTATAATT ACTCGGAAGCACTAACGTTTGATCCCCAGAAAGGCTTTTGGTGTCAGCTACTAGAAGGAGGAAAAACCAA ATGCCTTGGGAAAAGCAAAGGCCGAAAATACCCGCCCATGGATTCAGAGTCCAGAACTTTCCTCTCCAGC TACTACCGCGACCACAACGTGGAACTCTCGAAGCTGCTGCACAGGCTGGGGCAGCCTCTGCCCTCGTGGC TGAGACAGGAGCTGCAGAAAGTGAGGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI |
ACCN | NM_022565 |
Insert Size | 2619 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_022565.2, NP_072087.2 |
RefSeq Size | 3489 bp |
RefSeq ORF | 2619 bp |
Locus ID | 64580 |
UniProt ID | Q9EQW8 |
Gene Summary | Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Has low deacetylase activity but high sulfotransferase activity.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG218546 | Ndst4 (tGFP-tagged) - Mouse N-deacetylase/N-sulfotransferase (heparin glucosaminyl) 4 (Ndst4), (10ug) |
CNY 8,460.00 |
|
MR218546 | Ndst4 (Myc-DDK-tagged) - Mouse N-deacetylase/N-sulfotransferase (heparin glucosaminyl) 4 (Ndst4) |
CNY 6,384.00 |
|
MR218546L3 | Lenti ORF clone of Ndst4 (Myc-DDK-tagged) - Mouse N-deacetylase/N-sulfotransferase (heparin glucosaminyl) 4 (Ndst4) |
CNY 9,600.00 |
|
MR218546L4 | Lenti ORF clone of Ndst4 (mGFP-tagged) - Mouse N-deacetylase/N-sulfotransferase (heparin glucosaminyl) 4 (Ndst4) |
CNY 9,600.00 |