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Sik2 (NM_178710) Mouse Tagged ORF Clone
CAT＃: MG218168
- TrueORF®
Sik2 (tGFP-tagged) - Mouse salt inducible kinase 2 (Sik2), (10ug)
ORF Plasmid: DDK
"NM_178710" in other vectors (4)
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CNY 9,030.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | G630080D20Rik; Snf1lk2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG218168 representing NM_178710
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCATGGCGGATGGCCCGAGGCACTTGCAGCGCGGGCCAGTCCGGGTGGGGTTCTACGACATCGAGG GCACGCTGGGCAAGGGCAACTTTGCCGTGGTGAAGCTGGGGCGGCACCGGATCACCAAGACGGAGGTGGC TATAAAAATAATAGACAAGTCACAGCTGGATGCAGTAAACCTTGAGAAAATCTACCGAGAAGTACAGATA ATGAAAATGCTCGACCATCCTCACATCATTAAACTGTATCAGGTAATGGAGACCAAAAGTATGTTGTACC TTGTGACAGAATATGCCAAAAATGGAGAAATTTTTGATTATCTTGCTAATCATGGCCGATTAAATGAATC TGAAGCCAGGAGAAAATTCTGGCAAATTCTGTCTGCTGTTGATTATTGCCATGGCCGGAAGGTAGTGCAC AGAGACCTGAAGGCTGAAAATCTCCTGCTGGATAACAACATGAATATCAAAATAGCAGATTTCGGCTTTG GAAATTTCTTTAAAACTGGTGAACTGCTGGCAACATGGTGTGGCAGCCCCCCTTATGCAGCCCCAGAAGT CTTTGAAGGGCAGCAGTATGAAGGACCACAGCTGGATATATGGAGCATGGGAGTTGTTCTTTATGTCCTT GTCTGTGGAGCTCTACCTTTTGATGGACCAACTCTCCCTATTTTGAGGCAGAGGGTTTTAGAAGGAAGAT TCCGGATTCCTTATTTCATGTCAGAAGATTGTGAACACCTCATTAGAAGGATGTTGGTCCTAGATCCTTC CAAACGGCTAAGCATAGCTCAAATCAAGGAGCACAAGTGGATGCTCATAGAGGTTCCTGTACAGAGGCCT ATTCTCTACCCACAAGAACAGGAAAACGAGCCGTCCATTGGAGAATTTAATGAGCAGGTTCTTCGACTGA TGCACAGCCTTGGGATTGATCAGCAGAAGACTGTTGAGTCTTTGCAAAACAAGAGCTATAATCACTTTGC TGCCATTTATTTCTTGTTGGTGGAACGTCTAAAATCACATAGGAGCAGTTTTCCTGTGGAGCAGAGACTT GATGGCCGCCAGCGTCGGCCTAGCACCATTGCTGAACAAACAGTTGCCAAGGCACAAACTGTGGGGCTGC CAGTGACCTTGCATCCACCGAACGTGAGACTGATGCGATCTACCCTCCTCCCACAGGCATCCAATGTGGA GGCCTTTTCATTTCCAACATCCAGCTGTCAAGCAGAAGCTGCCTTTATGGAGGAAGAGTGTGTCGACACT CCAAAGGTGAATGGCTGCCTGCTTGACCCTGTGCCCCCTGTCCTGGTGAGGAAGGGATGCCAGTCACTGC CCAGCAGTATGATGGAGACCTCCATTGATGAAGGCTTGGAGACAGAAGGAGAGGCTGAGGAAGACCCCAG TCAGGCCTTTGAAGCTTTCCAGGCCACACGCAGTGGGCAGCGACGGCACACTCTGTCAGAAGTGACTAAC CAATTGGTTGTGATGCCCGGGGCAGGGAAAATGTTCTCCATGAGTGATAACCCCTCCCTTGAGAGTGTGG ACTCTGAGTATGATATGGGGTCTGCCCAGAGGGACCTGAACTTTCTGGAAGACAGCCCTTCCTTGAAGGA CATCATGTTAGCCAATCAGCCGTCACCCCGCATGACATCTCCTTTCATAAGCCTCAGACCTGCCAACCCA GCCATGCAGGCTCTGAGCTCACAGAAGCGTGAGGCCCACAACCGGTCCCCCGTGAGCTTCCGAGAAGGCC GCAGAGCATCAGATACGTCCCTTACACAAGGAATTGTAGCATTTAGACAACATCTTCAGAATCTTGCTAG AACCAAAGGAATTCTGGAGTTGAACAAAGTACAATTGCTGTATGAACAAATGGGATCAAACGCAGACCCT ACCTTAACATCAACTGCTCCTCAGCTCCAAGACCTTTCGAGCAGTTGCCCTCAGGAGGAAATCTCCCAGC AGCAGGAAAGTGTCTCCAGCCTGTCTGCCAGCATGCACCCTCAGCTCTCACCACAGCAAAGCTTGGAAAC CCAGTACCTACAGCATCGACTCCAGAAGCCCAATCTTCTGCCAAAGGCCCAGAGTCCTTGTCCAGTGTAT TGTAAAGAGCCACCTCGGAGCCTGGAACAGCAGCTACAGGAGCATAGGCTCCAACAGAAGCGACTCTTCC TCCAGAAGCAGTCTCAGCTGCAAGCATATTTTAATCAGATGCAGATAGCAGAGAGCTCCTACCCTGGACC AAGTCAGCAGCTGGCTCTTCCCCACCAGGAGACTCCACTGACATCCCAGCAGCCCCCATCATTCAGCCTG ACCCAGGCCCTGAGTCCTGTCCTCGAGCCCTCTTCTGAGCAGATGCAATTTAGCTCTTTCCTCAGCCAAT ACCCAGAGATGCAGTTGCAGCCACTGCCCTCTACTCCCGGCCCCCAGGCTCCACCTCCCTTACCCTCACA ATTGCAGCAACATCAGCAGCCACCACCCCCACCACCACCCCCTCCACCACAGCAGCCAGGAGCTGCTCCA ACCTCCTTACAGTTCTCCTATCAGACTTGTGAGCTGCCAAGCACCACTTCTTCTGTACCAAACTATCCTG CTTCCTGTCACTATCCTGTGGATGGAGCCCAGCAGAGCAACCTCACAGGGGCGGACTGTCCCAGGAGCTC AGGACTTCAGGACACCGCATCTAGCTATGACCCACTGGCCCTCTCTGAGCTCCCTGGACTCTTTGATTGT GAAATGGTAGAAGCTGTGGATCCACAACACAATGGGGTTGTGAGCTGCTTAGCCCGGGAGACC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218168 representing NM_178710
Red=Cloning site Green=Tags(s) MVMADGPRHLQRGPVRVGFYDIEGTLGKGNFAVVKLGRHRITKTEVAIKIIDKSQLDAVNLEKIYREVQI MKMLDHPHIIKLYQVMETKSMLYLVTEYAKNGEIFDYLANHGRLNESEARRKFWQILSAVDYCHGRKVVH RDLKAENLLLDNNMNIKIADFGFGNFFKTGELLATWCGSPPYAAPEVFEGQQYEGPQLDIWSMGVVLYVL VCGALPFDGPTLPILRQRVLEGRFRIPYFMSEDCEHLIRRMLVLDPSKRLSIAQIKEHKWMLIEVPVQRP ILYPQEQENEPSIGEFNEQVLRLMHSLGIDQQKTVESLQNKSYNHFAAIYFLLVERLKSHRSSFPVEQRL DGRQRRPSTIAEQTVAKAQTVGLPVTLHPPNVRLMRSTLLPQASNVEAFSFPTSSCQAEAAFMEEECVDT PKVNGCLLDPVPPVLVRKGCQSLPSSMMETSIDEGLETEGEAEEDPSQAFEAFQATRSGQRRHTLSEVTN QLVVMPGAGKMFSMSDNPSLESVDSEYDMGSAQRDLNFLEDSPSLKDIMLANQPSPRMTSPFISLRPANP AMQALSSQKREAHNRSPVSFREGRRASDTSLTQGIVAFRQHLQNLARTKGILELNKVQLLYEQMGSNADP TLTSTAPQLQDLSSSCPQEEISQQQESVSSLSASMHPQLSPQQSLETQYLQHRLQKPNLLPKAQSPCPVY CKEPPRSLEQQLQEHRLQQKRLFLQKQSQLQAYFNQMQIAESSYPGPSQQLALPHQETPLTSQQPPSFSL TQALSPVLEPSSEQMQFSSFLSQYPEMQLQPLPSTPGPQAPPPLPSQLQQHQQPPPPPPPPPPQQPGAAP TSLQFSYQTCELPSTTSSVPNYPASCHYPVDGAQQSNLTGADCPRSSGLQDTASSYDPLALSELPGLFDC EMVEAVDPQHNGVVSCLARET TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_178710 |
| ORF Size | 2793 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_178710.3, NP_848825.2 |
| RefSeq Size | 3561 bp |
| RefSeq ORF | 2796 bp |
| Locus ID | 235344 |
| UniProt ID | Q8CFH6 |
| Gene Summary | Phosphorylates 'Ser-789' of IRS1 in insulin-stimulated adipocytes, potentially modulating the efficiency of insulin signal transduction. Inhibits CREB activity by phosphorylating and inhibiting activity of TORCs, the CREB-specific coactivators, like CRTC2/TORC2 and CRTC3/TORC3 in response to cAMP signaling (PubMed:29211348).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222719 | Sik2 (untagged) - Mouse salt inducible kinase 2 (Sik2), (10ug) |
CNY 7,512.00 |
|
| MR218168 | Sik2 (Myc-DDK-tagged) - Mouse salt inducible kinase 2 (Sik2) |
CNY 6,816.00 |
|
| MR218168L3 | Lenti ORF clone of Sik2 (Myc-DDK-tagged) - Mouse salt inducible kinase 2 (Sik2) |
CNY 10,070.00 |
|
| MR218168L4 | Lenti ORF clone of Sik2 (mGFP-tagged) - Mouse salt inducible kinase 2 (Sik2) |
CNY 10,070.00 |
