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Uvssa (NM_001081101) Mouse Tagged ORF Clone
CAT＃: MG218039
- TrueORF®
Uvssa (tGFP-tagged) - Mouse RIKEN cDNA 4933407H18 gene (4933407H18Rik), (10ug)
ORF Plasmid: DDK
"NM_001081101" in other vectors (4)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4933407H18Rik; D330017J19Rik; Kiaa1530; mKIAA1530 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG218039 representing NM_001081101
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCAGAAACTTTCACAGTTGATAGAGGAGCTCACAACTTCAGGAGAATCCCAACTCAATGCTCAGA AAATGAAGGAACTGAAGAAAATTTGCAAGTCTTCCGAGGAGCAGCTGAGCCATGCCTACCGCCTGCTGAT CACACAGCTGACCCAGGGCCATGCCGAGATCCGCCTCTCGGCCTTCCAGATCGTGGATGAGCTCTTCACC CGCTCTCATCAGTTCAGAATGCTGCTTGTTTCTGACTTCCAGGAATTCCTCGAGCTCACACTGGGCACAG ATAGTGACCGTCCCCTGCCACCCCCTCGGGAGGCAGCTCAGAGGCTAAGGCAGGCAGCCATGCAAGCTGT GGAAGGTTGGAATGAGAAGTTTGGGCAGGCCTATAAGAAGCTAGCCTTGGGCTACCACTTCCTAAAACAC ACCAAAAAGGTAGATTTTCGGGATATAAATGTTAGGACTGTGGCAGAAAGGAAGCGAGAAGAGGAGAAGC AGAAGCACCTGGATAAAATCCACAGAGAAAGTGCAGACCGGGCTAAGAGGGAAATGGAAGAAATGTATGA TGAAATTGAATGCTGCCTGACAGAAGTGGAGAACTGCTTTAAGCTGCTGGTGCCCTTGGATTTTGTACCA TGCCCAGAGGATAAATTCTTTGGTGAAGCGTCTAGCATGACAGAGGGCTATGCTCCTTGCCCCTTGAGCC CGGACCTGGCCACTCCCCGTGAGTCTGGTCTCTCTGGACCCCAGGATGAAGAGCAGCCATGCTGTAGCAA GGACCTGGTTGCCTCTGCATACCATGTAGGATCTGTTGTTGGTCTGAAGGCACTACCCCAAACAGCCATG AAAGACTCCTCCAGGGATGAGGACGAACCCAGCGACCCGGATGATTTCCTGCGGAGCCATGGACTGGGTT CCCACAAGTACACGCTGGATGTTGAAGTCCCCTCAGATGGTCTGAAGGTTCAGGAGAATGAAGACAACCT TGCTGTGCTCCATGCTGCTCGGGACTCGCTCAAACTCATCCAGAACAAGTTTCTGCCAACTGTGTGCTCC TGGGTCCAGCGGTTTACCCGTGCAGGGACCTACAGTGCACATTTAAAACAGGCCATTGACCTGAAAATGG AACTGGAACTTGCCCTGAAGAAGTATGAAGAACTGAACATTGAGCCTGGGAGAGGACAGAGGAGCAGGAC AGAAGCACTGGAGGACAGTGAGGATGAGGATCAGGACTTTGTGGAGGTCCCAGAGAAAGAGGGTTATGAG CCTCGAATCCCTGACCATCTACGAGCTGAATATGGGCTGGAGCCAAAGGCCCCACTGAAGACTCTGGAGA AAGGTACAGCTGTATGTAAGTTACAGGAGAGGACCAGGATGAGAAGAGAAGAGGAGGCCTCAGACCCCAC CTCTGCAGCTGCCCAGATGTTGCGGCTCCAGGACTGCTTATCATCTCCCTCTCCCTCCTCTACCAGGGTA CTTCCTGGGCCAGAGGAAGCCCAGAAGCAAGCAGAGCGAGCCCGAGCACCCATTGTACCCTTCGGAGTGG ACCTGTGTTACTGGGGCCAGGAGCAGCTAACAGCTGGAAAGATTCTTAAATCTGACTCTCAACACCGCTT CTGGAAACCCAGCGAGGTGGAGGAAGAGGTGGACAGTGCCCATGTTTCTGAGATGCTCCATAGCCGACAC ATTACCTTTTCAGGGACGTTTGAGCCTGTGCAGCACAAGTGCCGGGCCCTGAGGCCTAATGGCAGGCTCT GCGAGCGCCAGGATCGACTGAAGTGCCCATTCCATGGGAAGATCATCCCCAGAGATGACAAGGGGCAGCC TCTCAACCCAGAAGACAGAGCCCGTGAACAAAGGCAGCAGCTTCAGCGGCAGCAGGCACATCCAGATTGG CAGGACCCTGAGTTTCTGAAGGACGTGGAAGCAGCCACAGGGGTGGACCTCGGTTCATCCAGGTCCAGCA AGAAAGGCAAAGGGAAAAAGAAGAAGCACCCTAACCTCACGGATCTGCGAGAGCGCACCAACACCGCCCG GGCCCGCCTTGAGAAGAAGGTCTTTGCCAAGGCAGCCGTGCAGAGAGTAGTTGCTGCCATGAACCAGATG GACCAGAAGAAACACGAGAAGTTTGCAAACCAATTTAATTATGCGCTGAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG218039 representing NM_001081101
Red=Cloning site Green=Tags(s) MDQKLSQLIEELTTSGESQLNAQKMKELKKICKSSEEQLSHAYRLLITQLTQGHAEIRLSAFQIVDELFT RSHQFRMLLVSDFQEFLELTLGTDSDRPLPPPREAAQRLRQAAMQAVEGWNEKFGQAYKKLALGYHFLKH TKKVDFRDINVRTVAERKREEEKQKHLDKIHRESADRAKREMEEMYDEIECCLTEVENCFKLLVPLDFVP CPEDKFFGEASSMTEGYAPCPLSPDLATPRESGLSGPQDEEQPCCSKDLVASAYHVGSVVGLKALPQTAM KDSSRDEDEPSDPDDFLRSHGLGSHKYTLDVEVPSDGLKVQENEDNLAVLHAARDSLKLIQNKFLPTVCS WVQRFTRAGTYSAHLKQAIDLKMELELALKKYEELNIEPGRGQRSRTEALEDSEDEDQDFVEVPEKEGYE PRIPDHLRAEYGLEPKAPLKTLEKGTAVCKLQERTRMRREEEASDPTSAAAQMLRLQDCLSSPSPSSTRV LPGPEEAQKQAERARAPIVPFGVDLCYWGQEQLTAGKILKSDSQHRFWKPSEVEEEVDSAHVSEMLHSRH ITFSGTFEPVQHKCRALRPNGRLCERQDRLKCPFHGKIIPRDDKGQPLNPEDRAREQRQQLQRQQAHPDW QDPEFLKDVEAATGVDLGSSRSSKKGKGKKKKHPNLTDLRERTNTARARLEKKVFAKAAVQRVVAAMNQM DQKKHEKFANQFNYALK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001081101 |
| ORF Size | 2151 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001081101.2, NP_001074570.1 |
| RefSeq Size | 7343 bp |
| RefSeq ORF | 2154 bp |
| Locus ID | 71101 |
| UniProt ID | Q9D479 |
| Gene Summary | Factor involved in transcription-coupled nucleotide excision repair (TC-NER) in response to UV damage. TC-NER allows RNA polymerase II-blocking lesions to be rapidly removed from the transcribed strand of active genes. Acts by promoting stabilization of ERCC6 by recruiting deubiquitinating enzyme USP7 to TC-NER complexes, preventing UV-induced degradation of ERCC6 by the proteasome. Interacts with the elongating form of RNA polymerase II (RNA pol IIo) and facilitates its ubiquitination at UV damage sites, leading to promote RNA pol IIo backtracking to allow access to the nucleotide excision repair machinery. Not involved in processing oxidative damage (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221014 | Uvssa (untagged) - Mouse RIKEN cDNA 4933407H18 gene (4933407H18Rik), (10ug) |
CNY 9,220.00 |
|
| MR218039 | Uvssa (Myc-DDK-tagged) - Mouse RIKEN cDNA 4933407H18 gene (4933407H18Rik) |
CNY 5,256.00 |
|
| MR218039L3 | Lenti ORF clone of Uvssa (Myc-DDK-tagged) - Mouse RIKEN cDNA 4933407H18 gene (4933407H18Rik) |
CNY 7,700.00 |
|
| MR218039L4 | Lenti ORF clone of Uvssa (mGFP-tagged) - Mouse RIKEN cDNA 4933407H18 gene (4933407H18Rik) |
CNY 7,700.00 |
