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Plb1 (NM_001081407) Mouse Tagged ORF Clone
CAT＃: MG217244
- TrueORF®
Plb1 (tGFP-tagged) - Mouse phospholipase B1 (Plb1) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_001081407" in other vectors (4)
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CNY 11,880.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4632413E21Rik; 4930433E17Rik; 4930539A06Rik; BC033606 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG217244 representing NM_001081407
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCTGTACCCAGGTGTGTCCCCGGTGGGACTGCTGCTGCTGCTACTGCTGGGACAAGGGCCCTCCC AAATCCATGGCTCTTCTGGAGAGAACACATTGGCATGGCAGTCCCAGCAAGTGTTTTGGACCCTGAAGAA TTTTCCATTCCCTTGCAAGCCAAAGAAGTTAGAATTGAGTGTGCTTTCTGAGTCAGTTCACTCTCTGAGA CCCTCAGACATTAAACTCGTGGCAGCCATCGGCAACCCAGAAATTCCTCTAGCCCCTGGGTCTGGCACGA TCAACATGGAGAAACCTCAAAGCATCAAGAACCAACCACAGGATGTATGCATGGGAATCATGACAGTTCT TTCAGATATCATCAGACATTTCAGTCCTTCTGTCCTGATGCCCACGTGTTCTCCTGGGAAGGGCACTGCA GTCCACACTACTGCTGAAGATTTGTGGATTCAGGCTAAAGAGCTGGTGAGGCGCCTGAAGGACAACCCGC AACTTGACTTTGAGAAGGACTGGAAACTCATCACTGTGTTCTTCAGTAACACAAGCCAGTGTCACCTGTG TCCCTCTGCTCAGCAGAAAAGTCACTTGATGAGGCACATGGAGATGCTGTGGGGGGTGCTGGATTACCTG CATCATGAGGTCCCCAGAGCATTTGTGAATTTGGTGGATCTCTCTGAGGTTTTGGCCATGGATCTTCAGC ATCAAGAGACTGGCTTCAGCCCTGCACCAGAGGTTTGCAAATGCACAGAGACAACGACGCTATCCAAAGC TGTCATGCAGTGGTCCTATCAGGAAGCCTGGGAAGATCTTCTGGCCTCTAGCAAGTTCAACAAGCATGAG ACCTTCGCGGTGGTTTTCCAGCCTTTCTTCGATGAAATTGAACCACCCTTGAAAAGGTCCTCGCCCCAGG ATCCCACCACACTCGCCCTCAGGATCTGGAATAGCATGATGGAACCAGTGGGTCAAAAGGATGGGCTACT CAATACTGCAGAGAGAAAGACAATGAAATGTCCCTCTGAGGAGAGTCCCTATCTGTTCACCTACAAAAAT AGCAACTACCAGGCCAGACGACTGAAACCTATAACAAAGCTTCAGATGAAAGAAGGATCAGAATTCACCT GTCCTGACAAGAACCCCTCGAACTCAATCCCCACAACAGTTCACAGCTTGCGGCCAGCTGATATCAAGAT CATAGGAGCATTGGGTGACTCGCTCACGGCAGGCAATGGAGCTGGGGCCAGCCCTTGGAATATCTTGGAT GTCTTAACTGAATACCGAGGCCTGTCCTGGAGTGTGGGCGGGGATGAGACCATCAAAACCGTGACCACCC TACCCAACATTCTCCGGGAATTCAACCCCTCCTTGAAGGGCTTTTCTGTTGGCACTGGGAAAGAAAGCAC TTCCCGAGCATCCTTCAACCAGGCCGTAGCAGGAGCCAAATCTGATGGCTTAGCTGGCCAGGCCAGAAAG CTGGTGGACCTGATGAAGGCAGACAAGACAATAAACTTTCAGGAAGACTGGAAGATAATCACTGTGTTTA TAGGAGGCAATGACCTCTGTGCTTCCTGCAGTAACTCGACTCGCTTTTCTCCCCAAAACTTCATAGACAA CATCAAGAACGCCCTGGACATCCTCCATGCAGAGGTTCCACGGGCCTTTGTGAACATGGCAATGGTGATG GAGATCACCCCCTTGAGAGAACTATTCAATGAACCTACAGTCTCATGCCCACGGAACATCCTCAGCCGAC TGTGTCCTTGTGTCCTGGGCCTTGGTGATAACTCAGAAGAACTTTCCAGCCTCGTTCAAAGGAACAGGGA TTATCAGAAGAAAACTGAAGAGCTCATTAATAGCGGACGATATGACACGAGGGATAATTTCACTGTGGTT GTCCAGCCCTTATTTGAAAATGTGTCCATGCCACGGACCCCGGAGGGCGTGCCCGACAAGTCTTTCTTTG CCCCTGACTGTTTCCACTTTAATGCCAAGACTCATGCCCGCTCAGCCATTGCCCTCTGGAAGAACATGCT GGAACCTGTGGGCCACAAGACAAGACATAATAATTTTGAAATCAAGGCCCCTATCGTGTGTCCGAACCAG GCCTCGCCATTTCTGAGCACTACCAAGAACAGCAACCTGGGTAATGGAACCTGGATGGTGTGCGAGGAGA GAGCCCCTTCTGCCTCACCACCAACCTCAGTGCATACCCTGAGACCTGCAGACATTCAAGTTGTGGCAGC TCTGGGAGACTCTCTGACTGCTGGCAATGGAATCAGCTCCCAAGAAGGTAACCTCACCGATGTCTCCACA CAGTATCGAGGACTGTCCTATAGTGCTGGTGGGGACAAGACCCTGGAGAATGTGACCACTTTGCCCAATA TCCTACGGAAATTTAATGGAAATCTCACAGGCTACTCAGTAGGAACCGGTGACTCCAGTTCCGCAAACGC TTTCCTTAACCAGGCTGTCCCTGGGGCGAAGGCTGAGAACCTTACAAGCCAAGTCCGGACTCTGGTTCAG AAGATGAAGAGTGACAACAGAGTGAACTTCAACCGAGACTGGAAGGTCATCACGGTGATGATCGGCGCCA GTGACTTGTGTGACTTCTGCACGGATTCGAATCATTACTCTGCAGCCAATTTTTTTGACCATCTCCAAAA TGCCTTGGACATTCTACATAAGGAGGTACCCAGAGCCCTGGTCAACCTTGTGGACTTCATTAACCCCAGT ATCATTCGGGAAGTATTCCTGAAGAACCCAGACAAGTGCCCAGTGAATCAGTCCAGTGTCCTGTGCAACT GTGTTCTGACCCCGAGGAAGGATTCCTATGAGCTGGCAAGGTTGGAGGCCTTCACCAAATCCTACCAGAG TAGCATGCTCCAACTGGTTGAGTCAGGCCGCTACGACACTCGAGAGGACTTCTCTGTGGTACTGCAGCCC TTTCTCCTCAACACCAAGCTCCCTGTCCTAGAGAACGGGAAACCAGATACATCCTTCTTTGCCCCAGACT GCATCCACCTAAACCAGAAGTTCCACACTCAGCTCGCGAGAGCCCTCTGGGCCAATATGCTTGAACCCCT GGGAAAGAAAACTGATACTTTGGACCCAAAAGGACACATATCTTTGGCCTGCCCCACCAAGGATCAGCCC TTCCTGAGAACCTTCCGAAACAGTAACTACAAGTATCCTACCAAGCCAGCCATTGAGAACTGGGGCAGTG ACTTCCTGTGCACAGAGAAGAGCCCTTCCAGCCAGGTACCCACCTCAGTTCATGAGCTCCGACCAGCAGA CATCAAGGTGGTGGCAGCAATGGGTGACTTTCTGACTACAGCCACCGGAGCTCGGCCAAGTGGATATAAA AGGCTAGCCACGCCCTGGAGGGGGCTGTCTTGGAGCATTGGAGGAGATGGAAAGTTGGAGACCCATACCA CACTTCCCAACATCCTGAAGAAGTTCAACCCTTCCATCACTGGATTCTCTACTGGTACCCTGGACAACAA GGCAGGATTAAATGTGGCAGAAGAAGGAGCCAGAGCTCAGGACATGCCGGCCCAGGCTAAGACCCTGGTG AAGAAGATGAAAAGCACACCTACAATCAACCTACAGGAAGACTGGAAGCTGATTACACTCCTCATTGGAA ACAACGACCTGTGTCTTTACTGTGAGAATCCGGAGGACAACTCAACTAAAGAGTATGTCAAGTACATCCA GCAAGCCTTGGACATCCTCTATGAGGAGCTTCCCAGAGTTTTCATCAATGTGGTGGAAGTCATGGAGCTG GCTGGCCTGCACCACGTCCAAGGTGGGAAATGTGCCATGCCACTGGCAGTTCAGAAAAACTGCAGTTGCT TAAGACACTCCCAAAACCTCACAGCGATGCAGGAGCTGAAGAAACTAAACTGGAACCTCCAGAGCGGCAT CTCCGAGCTCTCCTATTGGCACCGGTACATGGAGCGTGAGGACTTCGCAGTCACTGTGCAGCCTTTCTTC CGGAATACCTTTATCCCACTGAATGAGCGTGAGGGCCTGGACCTCACTTTCTTCTCTGAAGACTGTTTCT ACTTCTCAGACCGTGGGCATGCTGAGATGGCCATTGCCCTCTGGAATAACATGCTGGAACCAGTGGGCTG GAAGACATCCTCCAATAACTTCATATACAACAGAACCAAACTCAAGTGCCCCTCACCTGAAAGGCCTTTC CTCTACACCCTCCGGAATAGTCAGCTTCTTCCAGACAAGGCTGAAGAACCCTCCAATGCACTCTACTGGG CAGTGCCAGTGGCAGCAATAGGTGGCCTGGCAGTTGGCATCCTTGGAGTGATGTTGTGGAGAACTGTGAA ACCCGTCCAACAGGAGGAGGAGGAGGAGGACACTCTTCCAAATACAAGTGTGACCCAGGATGCTGTATCA GAAAAGAGGCTCAAAGCTGGGAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG217244 representing NM_001081407
Red=Cloning site Green=Tags(s) MELYPGVSPVGLLLLLLLGQGPSQIHGSSGENTLAWQSQQVFWTLKNFPFPCKPKKLELSVLSESVHSLR PSDIKLVAAIGNPEIPLAPGSGTINMEKPQSIKNQPQDVCMGIMTVLSDIIRHFSPSVLMPTCSPGKGTA VHTTAEDLWIQAKELVRRLKDNPQLDFEKDWKLITVFFSNTSQCHLCPSAQQKSHLMRHMEMLWGVLDYL HHEVPRAFVNLVDLSEVLAMDLQHQETGFSPAPEVCKCTETTTLSKAVMQWSYQEAWEDLLASSKFNKHE TFAVVFQPFFDEIEPPLKRSSPQDPTTLALRIWNSMMEPVGQKDGLLNTAERKTMKCPSEESPYLFTYKN SNYQARRLKPITKLQMKEGSEFTCPDKNPSNSIPTTVHSLRPADIKIIGALGDSLTAGNGAGASPWNILD VLTEYRGLSWSVGGDETIKTVTTLPNILREFNPSLKGFSVGTGKESTSRASFNQAVAGAKSDGLAGQARK LVDLMKADKTINFQEDWKIITVFIGGNDLCASCSNSTRFSPQNFIDNIKNALDILHAEVPRAFVNMAMVM EITPLRELFNEPTVSCPRNILSRLCPCVLGLGDNSEELSSLVQRNRDYQKKTEELINSGRYDTRDNFTVV VQPLFENVSMPRTPEGVPDKSFFAPDCFHFNAKTHARSAIALWKNMLEPVGHKTRHNNFEIKAPIVCPNQ ASPFLSTTKNSNLGNGTWMVCEERAPSASPPTSVHTLRPADIQVVAALGDSLTAGNGISSQEGNLTDVST QYRGLSYSAGGDKTLENVTTLPNILRKFNGNLTGYSVGTGDSSSANAFLNQAVPGAKAENLTSQVRTLVQ KMKSDNRVNFNRDWKVITVMIGASDLCDFCTDSNHYSAANFFDHLQNALDILHKEVPRALVNLVDFINPS IIREVFLKNPDKCPVNQSSVLCNCVLTPRKDSYELARLEAFTKSYQSSMLQLVESGRYDTREDFSVVLQP FLLNTKLPVLENGKPDTSFFAPDCIHLNQKFHTQLARALWANMLEPLGKKTDTLDPKGHISLACPTKDQP FLRTFRNSNYKYPTKPAIENWGSDFLCTEKSPSSQVPTSVHELRPADIKVVAAMGDFLTTATGARPSGYK RLATPWRGLSWSIGGDGKLETHTTLPNILKKFNPSITGFSTGTLDNKAGLNVAEEGARAQDMPAQAKTLV KKMKSTPTINLQEDWKLITLLIGNNDLCLYCENPEDNSTKEYVKYIQQALDILYEELPRVFINVVEVMEL AGLHHVQGGKCAMPLAVQKNCSCLRHSQNLTAMQELKKLNWNLQSGISELSYWHRYMEREDFAVTVQPFF RNTFIPLNEREGLDLTFFSEDCFYFSDRGHAEMAIALWNNMLEPVGWKTSSNNFIYNRTKLKCPSPERPF LYTLRNSQLLPDKAEEPSNALYWAVPVAAIGGLAVGILGVMLWRTVKPVQQEEEEEDTLPNTSVTQDAVS EKRLKAGN TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001081407 |
| ORF Size | 4434 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001081407.1, NP_001074876.1 |
| RefSeq Size | 4853 bp |
| RefSeq ORF | 4437 bp |
| Locus ID | 665270 |
| UniProt ID | Q3TTY0 |
| Gene Summary | Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activity toward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224540 | Plb1 (untagged) - Mouse phospholipase B1 (Plb1), transcript variant 1, (10ug) |
CNY 18,910.00 |
|
| MR217244 | Plb1 (Myc-DDK-tagged) - Mouse phospholipase B1 (Plb1), transcript variant 1 |
CNY 10,112.00 |
|
| MR217244L3 | Lenti ORF clone of Plb1 (Myc-DDK-tagged) - Mouse phospholipase B1 (Plb1), transcript variant 1 |
CNY 12,730.00 |
|
| MR217244L4 | Lenti ORF clone of Plb1 (mGFP-tagged) - Mouse phospholipase B1 (Plb1), transcript variant 1 |
CNY 12,730.00 |
