Med15 (NM_033609) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	A230074L19Rik; AW536074; mPcqap; Pcqap
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG216942 representing NM_033609 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGTTTCGGGGCAGGAGACCGACTGGCGTAGCGCCGCCTTTCGGCAGAAGCTGGTCAGCCAAATTG AGGATGCCATGAGGAAAGCTGGTGTGGCCCACAGTAAATCTAGCAAGGATATGGAGAGTCATGTGTTCCT GAAGGCCAAGACCCGGGATGAGTATCTTTCCCTTGTGGCCCGACTCATTATCCATTTCCGAGATATTCAT AACAAGAAATCCCAAGCTTCTGTCAGTGACCCCATGAATGCACTGCAGAGCCTTACTGGTGGACCCACCC CAGGAGCAGCTGGGATTGGCATGCCTCCTCGGGGCCCAGGACAGTCCCTGGGTGGGATGGGTGGCCTTGG CGCTATGGGACAACCACTGCCCCTCTCCGGGCAACCACCCCCTGGAACCTCTGGAATGGCCCCTCATGGC ATGGCTGTGGTGTCTACAGCAACTCCACAGACTCAGCTGCAGCTCCAGCAAGTGGCATTGCAGCAACAGC AGCAGCAGCAACAACAACAGCAATTCCAGCAACAGCAGGCAGCACTGCAGCAACAGCAGCAACAGCAGCA GCAGCAACAGCAGCAACAGCAGTTCCAGGCACAACAGAATGCCATGCAGCAACAGTTCCAAGCAGTAGTG CAGCAGCAGCAGCTTCAGCAGCAGCAGCAGCAGCAGCACCTGATTAAGTTGCATCATCAAAGCCAGCAAC AACAGATACAACAGCAGCAACTGCAGAGGATGGCACAGTTGCAGCTGCAGCAACAGCAACAGCAGCAACA GCAGCAGGCTTTGCAGGCCCAGCCACCAATGCAGCAGCCATCAATGCAGCAGCCACAGCCTCCCCCTTCT CAGGCCCTACCCCAGCAGCTGTCACAGCTGCATCATCCACAGCATCACCAGCCACCACCTCAGGCTCAGC AGTCCCCCATTGCTCAAAACCAACCACCACAGATCCCACCACAGTCACAGAGCCAGCCTTTGGTGTCACA AGCACAAGCCCTTCCTGGACCGATGCTGTATGCTGCCCAACAGCAGCTGAAATTTGTCCGTGCTCCGATG GTGGTCCAGCAGCCGCAAGTGCAGCCCCAGGTGCAGCAGGTGCAGCCCCAGGTGCAGCCGCAGGCAGCAG TGCAGGCAGCACAGTCTGCCCAGATGGTAGCTCCCGGCGTCCAGATGATTGCTGAAGCCTTGGCCCAAGG CGGGATGCACGTAAGAGCCCGGTTCCCGCCCACCTCCACCATGTCTGCTGGCCCGTCAAGCTCCATCTCT TTGGGCGGACAGCCCACAACACAGGTCAGCCAAAGCAGCCTCACCATGCTGTCCTCACCGTCACCAGGCC AGCAGGTGCAGACCCCACAGTCGATGCCACCTCCCCCACAGCCGTCCCCACAACCTGGCTCACAGCCCAA CTCTAATGTCAGCTCCGGCCCTGCCCCATCTCCCAGCAGCTTCCTGCCTAGCCCTTCACCACAGCCTTCT CAGAGCCCAGTGACAGCACGCACCCCACAGAACTTCAGCGTTCCTTCCCCTGGACCTTTAAACACCCCTG TGAACCCCAGCTCTGTCATGAGCCCAGCTGGCTCTAGCCAGGCTGAGGAGCAGCAGTACCTGGACAAGCT GAAGCAATTGTCCAAGTACATCGAGCCCCTGCGACGCATGATCAACAAGATCGACAAGAATGAAGACAGA AAAAAGGACTTAAGTAAGATGAAGAGCCTGCTGGACATCCTCACCGACCCCTCGAAGAGGTGTCCCCTGA AGACCCTGCAAAAGTGTGAGATTGCGCTGGAGAAGCTCAAGAATGACATGGCAGTGCCCACACCCCCACC ACCCCCAGTTCTTCCAACCAAACAGCAGGACCTGTGCCAACCACTCCTAGATGCAGTCCTGGCCAACATC CGTTCACCTGTCTTCAACCATTCCCTGTACCGCACATTTGTGCCAGCCATGATGGCCATCCATGGCCCAC CTATCGTGTCGCCAGTGGTGTGTTCCCGGAAGCGCCGGTTTGAGGAGGATGAGCGGCAGAGCATACCCAA TGTGCTGCAAGGTGAAGTGGCAAGGCTGGATCCCAAGTTCCTGGTGAACTTGGACCCTTCTCACTGCAGC AACAACGGTACTGTCCACCTGATCTGCAAGCTGGATGATAAGGACCTCCCTAGTGTGCCACCACTGGAGC TCAGTGTGCCTGCTGACTACCCTGCCCAGAGCCCAATGTGGATCGACCGTCAGTGGCAATATGATGCCAA CCCCTTTCTGCAGTCAGTGCACCGGTGCATGACCTCAAGGCTGCTGCAGCTCCCTGACAAGCACTCGGTC ACAGCGCTGCTCAACACCTGGGCCCAGAGCATCCACCAGGCCTGCCTCTCAGCTGCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG216942 representing NM_033609 Red=Cloning site Green=Tags(s) MDVSGQETDWRSAAFRQKLVSQIEDAMRKAGVAHSKSSKDMESHVFLKAKTRDEYLSLVARLIIHFRDIH NKKSQASVSDPMNALQSLTGGPTPGAAGIGMPPRGPGQSLGGMGGLGAMGQPLPLSGQPPPGTSGMAPHG MAVVSTATPQTQLQLQQVALQQQQQQQQQQQFQQQQAALQQQQQQQQQQQQQQQFQAQQNAMQQQFQAVV QQQQLQQQQQQQHLIKLHHQSQQQQIQQQQLQRMAQLQLQQQQQQQQQQALQAQPPMQQPSMQQPQPPPS QALPQQLSQLHHPQHHQPPPQAQQSPIAQNQPPQIPPQSQSQPLVSQAQALPGPMLYAAQQQLKFVRAPM VVQQPQVQPQVQQVQPQVQPQAAVQAAQSAQMVAPGVQMIAEALAQGGMHVRARFPPTSTMSAGPSSSIS LGGQPTTQVSQSSLTMLSSPSPGQQVQTPQSMPPPPQPSPQPGSQPNSNVSSGPAPSPSSFLPSPSPQPS QSPVTARTPQNFSVPSPGPLNTPVNPSSVMSPAGSSQAEEQQYLDKLKQLSKYIEPLRRMINKIDKNEDR KKDLSKMKSLLDILTDPSKRCPLKTLQKCEIALEKLKNDMAVPTPPPPPVLPTKQQDLCQPLLDAVLANI RSPVFNHSLYRTFVPAMMAIHGPPIVSPVVCSRKRRFEEDERQSIPNVLQGEVARLDPKFLVNLDPSHCS NNGTVHLICKLDDKDLPSVPPLELSVPADYPAQSPMWIDRQWQYDANPFLQSVHRCMTSRLLQLPDKHSV TALLNTWAQSIHQACLSAA TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_033609
ORF Size	2367 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_033609.3
RefSeq Size	3383 bp
RefSeq ORF	2370 bp
Locus ID	94112
UniProt ID	Q924H2
Gene Summary	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway (By similarity).[UniProtKB/Swiss-Prot Function]

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