Frk (NM_010237) Mouse Tagged ORF Clone
CAT#: MG216926
- TrueORF®
Frk (tGFP-tagged) - Mouse fyn-related kinase (Frk) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_010237" in other vectors (5)
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CNY 4,940.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | BSK; BSK/IYK; C85044; GTK; RAK |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG216926 representing NM_010237
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCAGCGTCTGTGTGAGACTCTGGGCATACCTGCAGCCTTTTCTCCCGTGCTGGTCTCAAGAGGCAG ACAAGTCAGTAGTAATTGAGAATCCAGGGGCCTTCTGTCCCCCAGAGGCTCCCAGGTCACAAGAGCCCGA GAGAAGCCATGGCCAGTATTTTGTGGCTCTGTTTGATTACCAAGCACGTACTGCAGAGGACCTGAGCTTC CGTGCCGGCGACAAACTCCAAGTCTTGGACACTTCGCATGAGGGCTGGTGGTTGGCCAGACATTTGGAGA AGAAGGGAACCGGCTTAGGTCAGCAGCTACAGGGCTACATTCCTTCCAATTACGTGGCGGAGGACCGGAG TCTCCAGGCAGAGCCGTGGTTTTTTGGAGCAATCAAAAGAGCAGATGCAGAAAAACAACTTCTGTATTCA GAAAACCAGACGGGCGCCTTTCTAATCAGAGAGAGTGAGAGCCAGAAGGGTGACTTTTCCCTCTCAGTTT TAGATGAAGGTGTTGTAAAACACTACAGAATAAGAAGGTTGGATGAAGGTGGCTTCTTCCTCACCAGGAG GAAAGTCTTTTCAACCCTGAATGAATTCGTGAACTACTACACCACAACAAGTGACGGGCTGTGTGTCAAG CTGGAGAAGCCATGCTTAAAGATCCAGGTACCAACCCCTTTTGATTTGTCATATAAAACTGCAGACCAGT GGGAGATAGACCGCAACTCCATACAGCTTTTGAAGCGACTGGGATCTGGTCAGTTTGGAGAAGTTTGGGA AGGTCTGTGGAATAATACCACTCCAGTGGCCGTAAAAACGTTAAAACCAGGTTCAATGGATCCAAATGAC TTCCTGAGGGAGGCACAGATAATGAAGAGCCTAAGACACCCAAAACTCATCCAGCTCTATGCTGTTTGCA CTTTAGAAGATCCCATTTATATTATTACAGAGTTGATGAGACATGGAAGCCTGCAAGAATATCTCCAAAA TGATGGTGGGTCAAAAATCCATTTGATTCAACAGGTAGACATGGCGGCACAGGTGGCTTCTGGAATGGCC TATCTTGAGTCGCAGAACTATATTCACAGAGATCTGGCTGCAAGAAATGTCCTTGTTGGTGAACATAATA TCTACAAAGTAGCAGATTTTGGACTTGCAAGAGTTTTTAAGGTAGATAATGAAGACATCTATGAATCTAA ACACGAAATAAAGCTGCCAGTGAAGTGGACTGCACCCGAAGCCATTCGTACTAATAAATTCAGCATTAAG TCTGATGTGTGGTCTTTTGGAATCCTGCTCTATGAAATCATTACTTATGGCAAAATGCCTTACAGTGGTA TGACAGGTGCTCAAGTAATTCAAATGTTGAGTCAAAACTACAGACTTCCACAGCCATCTAACTGCCCACA GCAATTCTACAGCATCATGCTAGAGTGCTGGAATGTTGAGCCTAAGCAACGGCCAACATTTGAGACCCTG CATTGGAAACTTGAAGACTACTTTGAAACAGACTGTTCCTATTCAGATACAAATAACTTCATAAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG216926 representing NM_010237
Red=Cloning site Green=Tags(s) MGSVCVRLWAYLQPFLPCWSQEADKSVVIENPGAFCPPEAPRSQEPERSHGQYFVALFDYQARTAEDLSF RAGDKLQVLDTSHEGWWLARHLEKKGTGLGQQLQGYIPSNYVAEDRSLQAEPWFFGAIKRADAEKQLLYS ENQTGAFLIRESESQKGDFSLSVLDEGVVKHYRIRRLDEGGFFLTRRKVFSTLNEFVNYYTTTSDGLCVK LEKPCLKIQVPTPFDLSYKTADQWEIDRNSIQLLKRLGSGQFGEVWEGLWNNTTPVAVKTLKPGSMDPND FLREAQIMKSLRHPKLIQLYAVCTLEDPIYIITELMRHGSLQEYLQNDGGSKIHLIQQVDMAAQVASGMA YLESQNYIHRDLAARNVLVGEHNIYKVADFGLARVFKVDNEDIYESKHEIKLPVKWTAPEAIRTNKFSIK SDVWSFGILLYEIITYGKMPYSGMTGAQVIQMLSQNYRLPQPSNCPQQFYSIMLECWNVEPKQRPTFETL HWKLEDYFETDCSYSDTNNFIN TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010237 |
ORF Size | 1536 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010237.3, NP_034367.2 |
RefSeq Size | 4669 bp |
RefSeq ORF | 1539 bp |
Locus ID | 14302 |
UniProt ID | Q922K9 |
Gene Summary | Non-receptor tyrosine-protein kinase that negatively regulates cell proliferation. Positively regulates PTEN protein stability through phosphorylation of PTEN on 'Tyr-336', which in turn prevents its ubiquitination and degradation, possibly by reducing its binding to NEDD4. May function as a tumor suppressor (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC217280 | Frk (untagged) - Mouse fyn-related kinase (Frk), transcript variant 2, (10ug) |
CNY 6,650.00 |
|
MG208226 | Frk (tGFP-tagged) - Mouse fyn-related kinase (Frk) |
CNY 4,940.00 |
|
MR216926 | Frk (Myc-DDK-tagged) - Mouse fyn-related kinase (Frk), transcript variant 2 |
CNY 3,824.00 |
|
MR216926L3 | Lenti ORF clone of Frk (Myc-DDK-tagged) - Mouse fyn-related kinase (Frk), transcript variant 2 |
CNY 6,370.00 |
|
MR216926L4 | Lenti ORF clone of Frk (mGFP-tagged) - Mouse fyn-related kinase (Frk), transcript variant 2 |
CNY 6,370.00 |