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Sptbn1 (NM_175836) Mouse Tagged ORF Clone
CAT＃: MG216353
- TrueORF®
Sptbn1 (tGFP-tagged) - Mouse spectrin beta 2 (Spnb2) transcript variant 1, (10ug)
ORF Plasmid: DDK
"NM_175836" in other vectors (2)
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CNY 21,280.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 9930031C03Rik; AL033301; elf1; elf3; mKIAA4049; Spnb-2; Spnb2; SPTB2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG216353 representing NM_175836
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGACCACGGTAGCCACAGACTATGACAACATTGAGATCCAGCAGCAGTACAGTGATGTGAACAACC GCTGGGATGTGGATGACTGGGACAATGAGAACAGCTCTGCAAGGCTCTTTGAGCGCTCCCGCATCAAGGC CCTGGCAGATGAGCGTGAAGCTGTACAGAAGAAGACCTTCACCAAGTGGGTCAATTCCCACCTTGCGAGA GTGTCCTGCCGAATCACAGACCTGTACACGGACCTTCGAGATGGACGGATGCTCATCAAGCTACTGGAGG TCCTCTCTGGAGAGAGGCTGCCTAAACCCACTAAGGGACGGATGCGGATCCACTGTCTGGAGAATGTCGA CAAGGCTCTTCAATTCCTGAAAGAGCAGAGAGTCCATCTTGAGAACATGGGCTCCCATGACATTGTGGAT GGAAACCACCGGCTGACCCTCGGCCTCATCTGGACAATTATTCTGCGCTTCCAGATCCAGGATATTAGTG TGGAGACTGAAGATAACAAAGAGAAAAAGTCTGCTAAGGATGCATTGCTGCTGTGGTGCCAGATGAAGAC AGCTGGGTACCCCAATGTCAACATTCACAATTTCACCACTAGCTGGAGGGATGGCATGGCCTTCAATGCA CTGATACATAAACATCGGCCTGACCTGATAGATTTTGATAAACTGAAGAAATCTAATGCACACTACAATC TGCAGAATGCATTTAACCTGGCAGAGCAGCACCTTGGCCTCACTAAACTGTTAGACCCTGAAGATATCAG TGTGGACCACCCTGATGAGAAGTCTATCATCACATACGTGGTGACTTACTACCACTACTTCTCCAAGATG AAGGCCTTGGCTGTCGAAGGAAAGCGCATTGGAAAGGTGCTTGATAATGCTATAGAAACAGAGAAAATGA TTGAGAAGTACGAGTCACTTGCTTCTGACCTTCTGGAGTGGATTGAACAAACCATCATCATCCTAAACAA CCGCAAATTTGCTAATTCACTGGTTGGGGTCCAGCAGCAGCTCCAGGCATTCAACACGTACCGCACAGTG GAGAAACCACCTAAGTTTACTGAGAAGGGGAATTTGGAGGTGCTCCTTTTCACAATTCAGAGCAAGATGC GAGCGAATAATCAGAAGGTCTACATGCCCCGCGAGGGGAAGCTCATCTCTGACATCAACAAGGCCTGGGA AAGACTGGAAAAAGCAGAACATGAGAGAGAACTGGCTCTGCGGAATGAGCTCATACGGCAGGAAAAACTG GAACAACTCGCCCGAAGATTTGATCGCAAGGCAGCTATGAGGGAGACATGGCTGAGTGAAAACCAGCGTC TTGTGTCTCAGGACAACTTTGGATTTGACCTTCCCGCTGTTGAGGCTGCTACCAAAAAACACGAGGCCAT TGAGACAGACATCGCTGCATATGAAGAACGAGTTCAGGCCGTGGTGGCTGTGGCCAGGGAACTTGAAGCC GAGAACTACCATGACATCAAGCGCATCACAGCGAGGAAGGACAATGTCATCCGGCTCTGGGAATACTTGC TGGAACTGCTCAGGGCCAGGAGGCAGCGTCTTGAGATGAACCTGGGATTGCAAAAGATATTCCAGGAAAT GCTTTATATTATGGACTGGATGGATGAAATGAAGGTGCTATTGCTGTCTCAAGACTATGGCAAACACTTA CTTGGTGTTGAAGACCTGTTACAGAAGCATGCCCTGGTTGAAGCAGACATTGCAATCCAAGCAGAGCGTG TAAGAGGTGTGAATGCCTCTGCCCAGAAGTTTGCAACAGATGGGGAAGGCTACAAGCCATGTGACCCCCA GGTAATTCGAGACCGTGTTGCCCACATGGAGTTCTGCTATCAAGAGCTTTGTCAGCTGGCTGCCGAGCGT AGGGCTCGCCTGGAAGAGTCCCGTCGCCTCTGGAAGTTCTTCTGGGAGATGGCAGAAGAGGAAGGCTGGA TACGAGAGAAGGAGAAGATCCTGTCCTCTGATGATTACGGGAAAGACTTGACCAGTGTCATGCGCCTGCT GAGCAAGCACCGGGCATTTGAGGATGAGATGAGTGGCCGTAGTGGCCATTTTGAGCAGGCCATTAAAGAA GGTGAAGACATGATTGCAGAGGAACACTTTGGATCGGAAAAGATCCGTGAGAGAATCATTTATATCCGGG AGCAGTGGGCCAACCTGGAACAGCTCTCAGCCATTAGGAAGAAGCGCCTAGAGGAAGCCTCATTACTGCA CCAGTTCCAGGCTGATGCTGATGATATTGATGCTTGGATGTTAGATATACTCAAGATTGTCTCCAGCAAT GATGTGGGCCATGATGAGTACTCCACGCAGTCTCTGGTCAAGAAGCATAAAGATGTAGCAGAAGAGATCA CCAACTACAGGCCCACTATTGACACACTGCATGAGCAAGCCAGTGCCCTTCCACAAGCACATGCAGAGTC TCCAGATGTGAAGGGCCGGCTGGCAGGAATTGAGGAGCGCTGCAAGGAGATGGCAGAGTTAACACGGCTA AGGAAGCAGGCTCTGCAGGACACCCTGGCCCTGTACAAGATGTTCAGTGAGGCTGATGCCTGTGAGCTCT GGATTGACGAGAAGGAGCAGTGGCTCAACAACATGCAGATCCCAGAGAAGCTGGAGGACCTGGAAGTCAT CCAGCACAGATTTGAGAGCCTAGAACCAGAAATGAACAACCAGGCTTCCCGGGTTGCTGTGGTGAACCAG ATTGCACGGCAGCTGATGCACAATGGCCACCCCAGTGAAAAGGAAATCAGAGCTCAGCAAGACAAACTCA ACACGAGGTGGAGTCAGTTCAGAGAACTGGTGGACAGGAAAAAGGATGCTCTTCTGTCTGCCCTGAGCAT CCAGAACTACCACCTCGAGTGCAATGAAACCAAATCCTGGATCCGGGAGAAGACCAAGGTCATCGAGTCT ACCCAAGACCTTGGCAATGACCTGGCAGGTGTCATGGCCCTGCAGCGCAAGCTGACTGGCATGGAACGAG ACTTGGTAGCCATTGAGGCGAAGCTGAGTGACCTGCAGAAAGAAGCTGAGAAGCTGGAGTCCGAGCACCC TGACCAGGCTCAAGCTATCCTGTCTCGGCTGGCCGAGATCAGTGATGTGTGGGAGGAAATGAAGACAACC CTGAAGAACCGAGAGGCCTCCCTGGGAGAGGCCAGCAAGCTGCAGCAGTTTCTGCGGGACTTGGACGACT TCCAGTCTTGGCTCTCCAGGACCCAGACTGCTATCGCCTCAGAGGACATGCCCAATACCCTCACTGAGGC AGAGAAGCTTCTCACACAGCACGAGAATATCAAAAATGAGATCGACAATTATGAGGAAGACTACCAGAAG ATGCGGGACATGGGCGAGATGGTCACCCAGGGGCAGACTGATGCCCAGTATATGTTTCTGCGGCAGCGGC TGCAGGCCTTAGACACTGGCTGGAATGAGCTCCACAAAATGTGGGAGAACAGGCAAAACCTCCTCTCCCA GTCCCATGCCTACCAGCAGTTCCTTAGGGACACCAAACAAGCTGAAGCTTTTCTTAATAACCAGGAGTAT GTTTTGGCTCATACTGAAATGCCCACCACCCTGGAAGGAGCTGAAGCAGCCATTAAAAAGCAGGAGGACT TCATGACCACCATGGATGCCAACGAGGAGAAGATCAATGCTGTTGTGGAGACTGGCCGAAGACTGGTGAG CGATGGGAACATCAACTCCGACCGCATCCAGGAGAAGGTGGACTCTATTGACGACAGACACAGGAAGAAT CGAGAAGCAGCCAGTGAACTTCTGATGAGGTTAAAGGACAACCGTGATCTACAGAAGTTCCTGCAAGATT GTCAAGAGCTGTCCCTCTGGATCAATGAAAAGATGCTTACAGCTCAAGACATGTCTTATGATGAAGCCAG AAATCTGCACAGTAAATGGTTAAAGCATCAAGCATTTATGGCGGAACTTGCATCCAACAAAGAATGGCTT GACAAAATTGAGAAGGAAGGAATGCAGCTTATTTCAGAAAAGCCAGAAACAGAAGCTGTGGTAAAGGAAA AACTCACTGGTTTACATAAAATGTGGGAAGTCCTTGAATCCACAACCCAGACCAAGGCCCAGCGGCTCTT TGATGCAAATAAGGCTGAGCTTTTCACACAAAGCTGCGCAGATCTTGACAAATGGCTACATGGCCTGGAG AGCCAGATTCAATCTGACGACTATGGCAAAGACCTTACCAGTGTCAATATTCTTCTGAAAAAGCAACAGA TGCTGGAGAATCAGATGGAAGTTCGGAAGAAAGAGATCGAGGAACTGCAGAGCCAAGCCCAGGCGCTGAG TCAGGAGGGGAAGAGCACAGATGAGGTGGACAGCAAACGCCTTACTGTGCAGACCAAGTTCATGGAGCTT CTGGAGCCCTTGAGTGAGAGGAAGCATAACCTGTTAGCTTCCAAGGAGATCCATCAGTTCAACAGGGATG TGGAGGACGAAATCCTATGGGTTGGCGAGAGGATGCCTTTGGCAACTTCCACAGATCATGGCCATAACCT TCAAACTGTGCAGCTGTTAATAAAGAAAAACCAGACCCTCCAGAAAGAAATCCAGGGACACCAGCCTCGT ATTGATGACATCTTTGAGAGGAGTCAAAACATCATCACAGATAGCAGCAGCCTCAATGCCGAGGCTATCA GGCAGAGGCTCGCTGACCTGAAGCAGCTGTGGGGGCTCCTCATTGAGGAAACTGAGAAACGCCATAGACG GCTGGAGGAGGCACACAAGGCGCAGCAGTACTACTTTGATGCAGCTGAAGCCGAGGCATGGATGAGTGAA CAGGAGTTGTACATGATGTCTGAGGAAAAGGCCAAGGATGAGCAGAGTGCTGTCTCTATGTTGAAAAAGC ACCAGATTTTAGAGCAAGCTGTTGAGGACTATGCAGAGACAGTACACCAGCTCTCCAAGACTAGCCGGGC GCTGGTGGCTGACAGCCATCCCGAAAGTGAGCGTATTAGCATGCGGCAGTCAAAGGTCGACAAGCTGTAT GCTGGCCTGAAGGACCTTGCTGAGGAGAGGAGAGGAAAACTTGATGAGAGGCACAGGCTGTTCCAGCTCA ACAGAGAGGTGGATGACCTGGAACAGTGGATCGCTGAGAGGGAAGTGGTCGCAGGCTCCCATGAGTTGGG ACAGGACTATGAGCATGTCACGATGTTACAAGAACGGTTCCGAGAATTTGCTCGAGACACAGGAAACATT GGGCAGGAGCGTGTGGATACAGTTAATAACATGGCAGATGAACTCATCAACTCTGGACATTCAGATGCTG CCACCATTGCTGAGTGGAAAGATGGTCTCAATGAAGCCTGGGCTGACCTCCTGGAGCTCATTGACACAAG AACACAGATTCTTGCTGCCTCATATGAACTTCATAAGTTTTACCATGATGCCAAGGAGATCTTTGGCCGA ATCCAGGACAAACACAAGAAACTCCCTGAGGAGCTTGGAAGAGATCAAAACACTGTGGAAACTTTACAGA GAATGCACACCACCTTTGAGCACGACATCCAAGCTCTGGGCACTCAGGTGAGGCAGCTGCAGGAGGATGC AGCTCGCCTCCAGGCAGCCTATGCAGGGGACAAGGCTGATGACATCCAGAAGCGTGAGAATGAGGTCCTG GAAGCCTGGAAGTCCCTGCTGGATGCTTGTGAGGGTCGCAGGGTGCGGCTGGTAGACACAGGAGACAAGT TCCGCTTCTTCAGCATGGTGCGTGACCTCATGCTCTGGATGGAAGATGTCATCCGGCAGATCGAGGCCCA GGAGAAACCACGGGATGTGTCATCTGTTGAACTGTTAATGAATAATCATCAAGGTATCAAAGCTGAAATT GATGCTCGTAATGACAGCTTTACAGCCTGCATTGAGCTTGGGAAATCCCTGCTGGCACGGAAACACTATG CTTCTGAGGAGATCAAGGAAAAGTTACTGCAGCTGACAGAGAAAAGAAAAGAAATGATTGACAAGTGGGA AGACCGGTGGGAGTGGTTAAGACTGATTTTGGAGGTCCATCAGTTCTCAAGGGATGCCAGTGTGGCAGAG GCTTGGCTGCTTGGACAGGAACCATACCTATCCAGCCGTGAAATTGGCCAGAGTGTAGACGAAGTGGAGA AGCTTATTAAGCGCCATGAGGCGTTTGAAAAGTCTGCAGCGACCTGGGATGAGAGATTCTCTGCTCTGGA AAGGCTGACAACGTTGGAGCTACTGGAAGTGCGCAGACAGCAAGAGGAAGAAGAAAGAAAGAGGCGGCCA CCTTCTCCGGACCCAAACACGAAGGTTTCAGAGGAGGCTGAGTCCCAGCAATGGGATACTTCAAAAGGAG ACCAAGTTTCCCAGAATGGTTTGCCGGCTGAGCAGGGATCTCCACGGATGGCAGGAACCATGGAAACGAG TGAAATGGTCAACGGTGCTGCTGAGCAGAGGACAAGCTCCAAAGAGTCCAGTCCTGTTCCCTCTCCCACC TTGGACCGAAAGGCCAAATCTGCACTTCCAGCCCAGAGTGCTGCCACCCTGCCAGCCAGGACCCTGGAGA CACCCGCTGCCCAGATGGAAGGCTTCCTCAATCGGAAGCATGAGTGGGAGGCCCACAATAAGAAAGCCTC GAGCAGGTCCTGGCACAATGTATATTGTGTCATAAATAACCAAGAAATGGGCTTCTATAAAGATGCCAAG AGTGCTGCTTCTGGCATCCCCTACCACAGTGAGGTCCCTGTGAGTTTGAAAGAGGCCATCTGCGAAGTGG CCCTTGATTACAAAAAGAAGAAGCACGTGTTCAAGCTAAGACTAAGTGATGGAAACGAGTACCTCTTCCA AGCCAAAGATGATGAGGAAATGAACACATGGATCCAGGCTATCTCCTCTGCCATCTCCTCTGACAAACAC GACACATCTGCCAGCACCCAGAGTACGCCAGCATCCAGTCGGGCGCAGACCTTACCCACCAGCGTCGTCA CCATCACCAGCGAGTCCAGTCCTGGCAAGAGGGAGAAGGATAAAGAGAAAGACAAAGAGAAGAGGTTCAG CCTTTTCGGCAAGAAGAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG216353 representing NM_175836
Red=Cloning site Green=Tags(s) MTTTVATDYDNIEIQQQYSDVNNRWDVDDWDNENSSARLFERSRIKALADEREAVQKKTFTKWVNSHLAR VSCRITDLYTDLRDGRMLIKLLEVLSGERLPKPTKGRMRIHCLENVDKALQFLKEQRVHLENMGSHDIVD GNHRLTLGLIWTIILRFQIQDISVETEDNKEKKSAKDALLLWCQMKTAGYPNVNIHNFTTSWRDGMAFNA LIHKHRPDLIDFDKLKKSNAHYNLQNAFNLAEQHLGLTKLLDPEDISVDHPDEKSIITYVVTYYHYFSKM KALAVEGKRIGKVLDNAIETEKMIEKYESLASDLLEWIEQTIIILNNRKFANSLVGVQQQLQAFNTYRTV EKPPKFTEKGNLEVLLFTIQSKMRANNQKVYMPREGKLISDINKAWERLEKAEHERELALRNELIRQEKL EQLARRFDRKAAMRETWLSENQRLVSQDNFGFDLPAVEAATKKHEAIETDIAAYEERVQAVVAVARELEA ENYHDIKRITARKDNVIRLWEYLLELLRARRQRLEMNLGLQKIFQEMLYIMDWMDEMKVLLLSQDYGKHL LGVEDLLQKHALVEADIAIQAERVRGVNASAQKFATDGEGYKPCDPQVIRDRVAHMEFCYQELCQLAAER RARLEESRRLWKFFWEMAEEEGWIREKEKILSSDDYGKDLTSVMRLLSKHRAFEDEMSGRSGHFEQAIKE GEDMIAEEHFGSEKIRERIIYIREQWANLEQLSAIRKKRLEEASLLHQFQADADDIDAWMLDILKIVSSN DVGHDEYSTQSLVKKHKDVAEEITNYRPTIDTLHEQASALPQAHAESPDVKGRLAGIEERCKEMAELTRL RKQALQDTLALYKMFSEADACELWIDEKEQWLNNMQIPEKLEDLEVIQHRFESLEPEMNNQASRVAVVNQ IARQLMHNGHPSEKEIRAQQDKLNTRWSQFRELVDRKKDALLSALSIQNYHLECNETKSWIREKTKVIES TQDLGNDLAGVMALQRKLTGMERDLVAIEAKLSDLQKEAEKLESEHPDQAQAILSRLAEISDVWEEMKTT LKNREASLGEASKLQQFLRDLDDFQSWLSRTQTAIASEDMPNTLTEAEKLLTQHENIKNEIDNYEEDYQK MRDMGEMVTQGQTDAQYMFLRQRLQALDTGWNELHKMWENRQNLLSQSHAYQQFLRDTKQAEAFLNNQEY VLAHTEMPTTLEGAEAAIKKQEDFMTTMDANEEKINAVVETGRRLVSDGNINSDRIQEKVDSIDDRHRKN REAASELLMRLKDNRDLQKFLQDCQELSLWINEKMLTAQDMSYDEARNLHSKWLKHQAFMAELASNKEWL DKIEKEGMQLISEKPETEAVVKEKLTGLHKMWEVLESTTQTKAQRLFDANKAELFTQSCADLDKWLHGLE SQIQSDDYGKDLTSVNILLKKQQMLENQMEVRKKEIEELQSQAQALSQEGKSTDEVDSKRLTVQTKFMEL LEPLSERKHNLLASKEIHQFNRDVEDEILWVGERMPLATSTDHGHNLQTVQLLIKKNQTLQKEIQGHQPR IDDIFERSQNIITDSSSLNAEAIRQRLADLKQLWGLLIEETEKRHRRLEEAHKAQQYYFDAAEAEAWMSE QELYMMSEEKAKDEQSAVSMLKKHQILEQAVEDYAETVHQLSKTSRALVADSHPESERISMRQSKVDKLY AGLKDLAEERRGKLDERHRLFQLNREVDDLEQWIAEREVVAGSHELGQDYEHVTMLQERFREFARDTGNI GQERVDTVNNMADELINSGHSDAATIAEWKDGLNEAWADLLELIDTRTQILAASYELHKFYHDAKEIFGR IQDKHKKLPEELGRDQNTVETLQRMHTTFEHDIQALGTQVRQLQEDAARLQAAYAGDKADDIQKRENEVL EAWKSLLDACEGRRVRLVDTGDKFRFFSMVRDLMLWMEDVIRQIEAQEKPRDVSSVELLMNNHQGIKAEI DARNDSFTACIELGKSLLARKHYASEEIKEKLLQLTEKRKEMIDKWEDRWEWLRLILEVHQFSRDASVAE AWLLGQEPYLSSREIGQSVDEVEKLIKRHEAFEKSAATWDERFSALERLTTLELLEVRRQQEEEERKRRP PSPDPNTKVSEEAESQQWDTSKGDQVSQNGLPAEQGSPRMAGTMETSEMVNGAAEQRTSSKESSPVPSPT LDRKAKSALPAQSAATLPARTLETPAAQMEGFLNRKHEWEAHNKKASSRSWHNVYCVINNQEMGFYKDAK SAASGIPYHSEVPVSLKEAICEVALDYKKKKHVFKLRLSDGNEYLFQAKDDEEMNTWIQAISSAISSDKH DTSASTQSTPASSRAQTLPTSVVTITSESSPGKREKDKEKDKEKRFSLFGKKK TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175836 |
| ORF Size | 7089 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175836.2, NP_787030.2 |
| RefSeq Size | 8265 bp |
| RefSeq ORF | 7092 bp |
| Locus ID | 20742 |
| UniProt ID | Q62261 |
| Gene Summary | Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.[UniProtKB/Swiss-Prot Function] |
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