Sptbn1 (NM_175836) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9930031C03Rik; AL033301; elf1; elf3; mKIAA4049; Spnb-2; Spnb2; SPTB2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225293 representing NM_175836 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGACCACGGTAGCCACAGACTATGACAACATTGAGATCCAGCAGCAGTACAGTGATGTGAACAACC GCTGGGATGTGGATGACTGGGACAATGAGAACAGCTCTGCAAGGCTCTTTGAGCGCTCCCGCATCAAGGC CCTGGCAGATGAGCGTGAAGCTGTACAGAAGAAGACCTTCACCAAGTGGGTCAATTCCCACCTTGCGAGA GTGTCCTGCCGAATCACAGACCTGTACACGGACCTTCGAGATGGACGGATGCTCATCAAGCTACTGGAGG TCCTCTCTGGAGAGAGGCTGCCTAAACCCACTAAGGGACGGATGCGGATCCACTGTCTGGAGAATGTCGA CAAGGCTCTTCAATTCCTGAAAGAGCAGAGAGTCCATCTTGAGAACATGGGCTCCCATGACATTGTGGAT GGAAACCACCGGCTGACCCTCGGCCTCATCTGGACAATTATTCTGCGCTTCCAGATCCAGGATATTAGTG TGGAGACTGAAGATAACAAAGAGAAAAAGTCTGCTAAGGATGCATTGCTGCTGTGGTGCCAGATGAAGAC AGCTGGGTACCCCAATGTCAACATTCACAATTTCACCACTAGCTGGAGGGATGGCATGGCCTTCAATGCA CTGATACATAAACATCGGCCTGACCTGATAGATTTTGATAAACTGAAGAAATCTAATGCACACTACAATC TGCAGAATGCATTTAACCTGGCAGAGCAGCACCTTGGCCTCACTAAACTGTTAGACCCTGAAGATATCAG TGTGGACCACCCTGATGAGAAGTCTATCATCACATACGTGGTGACTTACTACCACTACTTCTCCAAGATG AAGGCCTTGGCTGTCGAAGGAAAGCGCATTGGAAAGGTGCTTGATAATGCTATAGAAACAGAGAAAATGA TTGAGAAGTACGAGTCACTTGCTTCTGACCTTCTGGAGTGGATTGAACAAACCATCATCATCCTAAACAA CCGCAAATTTGCTAATTCACTGGTTGGGGTCCAGCAGCAGCTCCAGGCATTCAACACGTACCGCACAGTG GAGAAACCACCTAAGTTTACTGAGAAGGGGAATTTGGAGGTGCTCCTTTTCACAATTCAGAGCAAGATGC GAGCGAATAATCAGAAGGTCTACATGCCCCGCGAGGGGAAGCTCATCTCTGACATCAACAAGGCCTGGGA AAGACTGGAAAAAGCAGAACATGAGAGAGAACTGGCTCTGCGGAATGAGCTCATACGGCAGGAAAAACTG GAACAACTCGCCCGAAGATTTGATCGCAAGGCAGCTATGAGGGAGACATGGCTGAGTGAAAACCAGCGTC TTGTGTCTCAGGACAACTTTGGATTTGACCTTCCCGCTGTTGAGGCTGCTACCAAAAAACACGAGGCCAT TGAGACAGACATCGCTGCATATGAAGAACGAGTTCAGGCCGTGGTGGCTGTGGCCAGGGAACTTGAAGCC GAGAACTACCATGACATCAAGCGCATCACAGCGAGGAAGGACAATGTCATCCGGCTCTGGGAATACTTGC TGGAACTGCTCAGGGCCAGGAGGCAGCGTCTTGAGATGAACCTGGGATTGCAAAAGATATTCCAGGAAAT GCTTTATATTATGGACTGGATGGATGAAATGAAGGTGCTATTGCTGTCTCAAGACTATGGCAAACACTTA CTTGGTGTTGAAGACCTGTTACAGAAGCATGCCCTGGTTGAAGCAGACATTGCAATCCAAGCAGAGCGTG TAAGAGGTGTGAATGCCTCTGCCCAGAAGTTTGCAACAGATGGGGAAGGCTACAAGCCATGTGACCCCCA GGTAATTCGAGACCGTGTTGCCCACATGGAGTTCTGCTATCAAGAGCTTTGTCAGCTGGCTGCCGAGCGT AGGGCTCGCCTGGAAGAGTCCCGTCGCCTCTGGAAGTTCTTCTGGGAGATGGCAGAAGAGGAAGGCTGGA TACGAGAGAAGGAGAAGATCCTGTCCTCTGATGATTACGGGAAAGACTTGACCAGTGTCATGCGCCTGCT GAGCAAGCACCGGGCATTTGAGGATGAGATGAGTGGCCGTAGTGGCCATTTTGAGCAGGCCATTAAAGAA GGTGAAGACATGATTGCAGAGGAACACTTTGGATCGGAAAAGATCCGTGAGAGAATCATTTATATCCGGG AGCAGTGGGCCAACCTGGAACAGCTCTCAGCCATTAGGAAGAAGCGCCTAGAGGAAGCCTCATTACTGCA CCAGTTCCAGGCTGATGCTGATGATATTGATGCTTGGATGTTAGATATACTCAAGATTGTCTCCAGCAAT GATGTGGGCCATGATGAGTACTCCACGCAGTCTCTGGTCAAGAAGCATAAAGATGTAGCAGAAGAGATCA CCAACTACAGGCCCACTATTGACACACTGCATGAGCAAGCCAGTGCCCTTCCACAAGCACATGCAGAGTC TCCAGATGTGAAGGGCCGGCTGGCAGGAATTGAGGAGCGCTGCAAGGAGATGGCAGAGTTAACACGGCTA AGGAAGCAGGCTCTGCAGGACACCCTGGCCCTGTACAAGATGTTCAGTGAGGCTGATGCCTGTGAGCTCT GGATTGACGAGAAGGAGCAGTGGCTCAACAACATGCAGATCCCAGAGAAGCTGGAGGACCTGGAAGTCAT CCAGCACAGATTTGAGAGCCTAGAACCAGAAATGAACAACCAGGCTTCCCGGGTTGCTGTGGTGAACCAG ATTGCACGGCAGCTGATGCACAATGGCCACCCCAGTGAAAAGGAAATCAGAGCTCAGCAAGACAAACTCA ACACGAGGTGGAGTCAGTTCAGAGAACTGGTGGACAGGAAAAAGGATGCTCTTCTGTCTGCCCTGAGCAT CCAGAACTACCACCTCGAGTGCAATGAAACCAAATCCTGGATCCGGGAGAAGACCAAGGTCATCGAGTCT ACCCAAGACCTTGGCAATGACCTGGCAGGTGTCATGGCCCTGCAGCGCAAGCTGACTGGCATGGAACGAG ACTTGGTAGCCATTGAGGCGAAGCTGAGTGACCTGCAGAAAGAAGCTGAGAAGCTGGAGTCCGAGCACCC TGACCAGGCTCAAGCTATCCTGTCTCGGCTGGCCGAGATCAGTGATGTGTGGGAGGAAATGAAGACAACC CTGAAGAACCGAGAGGCCTCCCTGGGAGAGGCCAGCAAGCTGCAGCAGTTTCTGCGGGACTTGGACGACT TCCAGTCTTGGCTCTCCAGGACCCAGACTGCTATCGCCTCAGAGGACATGCCCAATACCCTCACTGAGGC AGAGAAGCTTCTCACACAGCACGAGAATATCAAAAATGAGATCGACAATTATGAGGAAGACTACCAGAAG ATGCGGGACATGGGCGAGATGGTCACCCAGGGGCAGACTGATGCCCAGTATATGTTTCTGCGGCAGCGGC TGCAGGCCTTAGACACTGGCTGGAATGAGCTCCACAAAATGTGGGAGAACAGGCAAAACCTCCTCTCCCA GTCCCATGCCTACCAGCAGTTCCTTAGGGACACCAAACAAGCTGAAGCTTTTCTTAATAACCAGGAGTAT GTTTTGGCTCATACTGAAATGCCCACCACCCTGGAAGGAGCTGAAGCAGCCATTAAAAAGCAGGAGGACT TCATGACCACCATGGATGCCAACGAGGAGAAGATCAATGCTGTTGTGGAGACTGGCCGAAGACTGGTGAG CGATGGGAACATCAACTCCGACCGCATCCAGGAGAAGGTGGACTCTATTGACGACAGACACAGGAAGAAT CGAGAAGCAGCCAGTGAACTTCTGATGAGGTTAAAGGACAACCGTGATCTACAGAAGTTCCTGCAAGATT GTCAAGAGCTGTCCCTCTGGATCAATGAAAAGATGCTTACAGCTCAAGACATGTCTTATGATGAAGCCAG AAATCTGCACAGTAAATGGTTAAAGCATCAAGCATTTATGGCGGAACTTGCATCCAACAAAGAATGGCTT GACAAAATTGAGAAGGAAGGAATGCAGCTTATTTCAGAAAAGCCAGAAACAGAAGCTGTGGTAAAGGAAA AACTCACTGGTTTACATAAAATGTGGGAAGTCCTTGAATCCACAACCCAGACCAAGGCCCAGCGGCTCTT TGATGCAAATAAGGCTGAGCTTTTCACACAAAGCTGCGCAGATCTTGACAAATGGCTACATGGCCTGGAG AGCCAGATTCAATCTGACGACTATGGCAAAGACCTTACCAGTGTCAATATTCTTCTGAAAAAGCAACAGA TGCTGGAGAATCAGATGGAAGTTCGGAAGAAAGAGATCGAGGAACTGCAGAGCCAAGCCCAGGCGCTGAG TCAGGAGGGGAAGAGCACAGATGAGGTGGACAGCAAACGCCTTACTGTGCAGACCAAGTTCATGGAGCTT CTGGAGCCCTTGAGTGAGAGGAAGCATAACCTGTTAGCTTCCAAGGAGATCCATCAGTTCAACAGGGATG TGGAGGACGAAATCCTATGGGTTGGCGAGAGGATGCCTTTGGCAACTTCCACAGATCATGGCCATAACCT TCAAACTGTGCAGCTGTTAATAAAGAAAAACCAGACCCTCCAGAAAGAAATCCAGGGACACCAGCCTCGT ATTGATGACATCTTTGAGAGGAGTCAAAACATCATCACAGATAGCAGCAGCCTCAATGCCGAGGCTATCA GGCAGAGGCTCGCTGACCTGAAGCAGCTGTGGGGGCTCCTCATTGAGGAAACTGAGAAACGCCATAGACG GCTGGAGGAGGCACACAAGGCGCAGCAGTACTACTTTGATGCAGCTGAAGCCGAGGCATGGATGAGTGAA CAGGAGTTGTACATGATGTCTGAGGAAAAGGCCAAGGATGAGCAGAGTGCTGTCTCTATGTTGAAAAAGC ACCAGATTTTAGAGCAAGCTGTTGAGGACTATGCAGAGACAGTACACCAGCTCTCCAAGACTAGCCGGGC GCTGGTGGCTGACAGCCATCCCGAAAGTGAGCGTATTAGCATGCGGCAGTCAAAGGTCGACAAGCTGTAT GCTGGCCTGAAGGACCTTGCTGAGGAGAGGAGAGGAAAACTTGATGAGAGGCACAGGCTGTTCCAGCTCA ACAGAGAGGTGGATGACCTGGAACAGTGGATCGCTGAGAGGGAAGTGGTCGCAGGCTCCCATGAGTTGGG ACAGGACTATGAGCATGTCACGATGTTACAAGAACGGTTCCGAGAATTTGCTCGAGACACAGGAAACATT GGGCAGGAGCGTGTGGATACAGTTAATAACATGGCAGATGAACTCATCAACTCTGGACATTCAGATGCTG CCACCATTGCTGAGTGGAAAGATGGTCTCAATGAAGCCTGGGCTGACCTCCTGGAGCTCATTGACACAAG AACACAGATTCTTGCTGCCTCATATGAACTTCATAAGTTTTACCATGATGCCAAGGAGATCTTTGGCCGA ATCCAGGACAAACACAAGAAACTCCCTGAGGAGCTTGGAAGAGATCAAAACACTGTGGAAACTTTACAGA GAATGCACACCACCTTTGAGCACGACATCCAAGCTCTGGGCACTCAGGTGAGGCAGCTGCAGGAGGATGC AGCTCGCCTCCAGGCAGCCTATGCAGGGGACAAGGCTGATGACATCCAGAAGCGTGAGAATGAGGTCCTG GAAGCCTGGAAGTCCCTGCTGGATGCTTGTGAGGGTCGCAGGGTGCGGCTGGTAGACACAGGAGACAAGT TCCGCTTCTTCAGCATGGTGCGTGACCTCATGCTCTGGATGGAAGATGTCATCCGGCAGATCGAGGCCCA GGAGAAACCACGGGATGTGTCATCTGTTGAACTGTTAATGAATAATCATCAAGGTATCAAAGCTGAAATT GATGCTCGTAATGACAGCTTTACAGCCTGCATTGAGCTTGGGAAATCCCTGCTGGCACGGAAACACTATG CTTCTGAGGAGATCAAGGAAAAGTTACTGCAGCTGACAGAGAAAAGAAAAGAAATGATTGACAAGTGGGA AGACCGGTGGGAGTGGTTAAGACTGATTTTGGAGGTCCATCAGTTCTCAAGGGATGCCAGTGTGGCAGAG GCTTGGCTGCTTGGACAGGAACCATACCTATCCAGCCGTGAAATTGGCCAGAGTGTAGACGAAGTGGAGA AGCTTATTAAGCGCCATGAGGCGTTTGAAAAGTCTGCAGCGACCTGGGATGAGAGATTCTCTGCTCTGGA AAGGCTGACAACGTTGGAGCTACTGGAAGTGCGCAGACAGCAAGAGGAAGAAGAAAGAAAGAGGCGGCCA CCTTCTCCGGACCCAAACACGAAGGTTTCAGAGGAGGCTGAGTCCCAGCAATGGGATACTTCAAAAGGAG ACCAAGTTTCCCAGAATGGTTTGCCGGCTGAGCAGGGATCTCCACGGATGGCAGGAACCATGGAAACGAG TGAAATGGTCAACGGTGCTGCTGAGCAGAGGACAAGCTCCAAAGAGTCCAGTCCTGTTCCCTCTCCCACC TTGGACCGAAAGGCCAAATCTGCACTTCCAGCCCAGAGTGCTGCCACCCTGCCAGCCAGGACCCTGGAGA CACCCGCTGCCCAGATGGAAGGCTTCCTCAATCGGAAGCATGAGTGGGAGGCCCACAATAAGAAAGCCTC GAGCAGGTCCTGGCACAATGTATATTGTGTCATAAATAACCAAGAAATGGGCTTCTATAAAGATGCCAAG AGTGCTGCTTCTGGCATCCCCTACCACAGTGAGGTCCCTGTGAGTTTGAAAGAGGCCATCTGCGAAGTGG CCCTTGATTACAAAAAGAAGAAGCACGTGTTCAAGCTAAGACTAAGTGATGGAAACGAGTACCTCTTCCA AGCCAAAGATGATGAGGAAATGAACACATGGATCCAGGCTATCTCCTCTGCCATCTCCTCTGACAAACAC GACACATCTGCCAGCACCCAGAGTACGCCAGCATCCAGTCGGGCGCAGACCTTACCCACCAGCGTCGTCA CCATCACCAGCGAGTCCAGTCCTGGCAAGAGGGAGAAGGATAAAGAGAAAGACAAAGAGAAGAGGTTCAG CCTTTTCGGCAAGAAGAAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_175836
Insert Size	7092 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_175836.2, NP_787030.2
RefSeq Size	8265 bp
RefSeq ORF	7092 bp
Locus ID	20742
UniProt ID	Q62261
Gene Summary	Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and it encodes the longer protein (isoform 1).

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