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Ficd (NM_001010825) Mouse Tagged ORF Clone
CAT＃: MG216189
- TrueORF®
Ficd (tGFP-tagged) - Mouse FIC domain containing (Ficd), (10ug)
ORF Plasmid: DDK
"NM_001010825" in other vectors (4)
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CNY 3,710.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | D5Ertd40e; Hype |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG216189 representing NM_001010825
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATACTCATGCCAATGGCGTCGGTGGTGGCAGTGGCCGAACCCAAATGGGTCTCCGTCTGGGGCCGTT TCCTGTGGATGGCGCTGCTGAGCATGGCTCTGGGGTCGCTGCTGGCTCTGCTGCTGCCGCTGGGAGTTGT GGAAGAGCATTGCCTGGCTGTGCTCAGAGGTTTCCACCTGCTCAGGAGCAAACTGGACAGGGCACAGCCT GTGGTCCCCAAGTGCACCAGCCTATGCACAGAGCTCAGTGTCTCCTCCAGGGATGCAGGGCTGCTGACAG TCAAGACTACGGCGTCTCCAGCAGGGAAGCTGGAAGCCAAGGCCGCCCTAAACCAAGCCCTGGAGATGAA GCGTCAAGGCAAGAGAGGGAAAGCCCACAAGCTCTTCCTGCACGCCCTCAAGATGGACCCCGGCTTTGTA GACGCACTGAATGAGTTCGGCATCTTCTCCGAAGAAGACAAGGACATCATCCAGGCTGACTACTTATACA CCAGGGCCCTGACCATCTCGCCCTTCCACGAGAAAGCCCTGGTCAACCGGGATCGGACACTGCCCCTCGT GGAGGAAATCGACCAGAGGTACTTCAGCGTCATCGACAGCAAAGTGAAGAAGGTCATGTCCATCCCAAAG GGGAGCTCAGCGCTGCGCAGGGTCATGGAGGAGACCTACTACCACCACATCTACCACACGGTGGCCATCG AGGGCAACACCCTCACCCTCTCGGAGATCAGGCACATCCTGGAGACCCGCTACGCCGTGCCAGGGAAGAG CCTGGAAGAGCAGAACGAGGTGATCGGCATGCACGCGGCCATGAAGTACATCAACACCACCCTGGTCTCC CGCATCGGGTCTGTCACCATGGACGACATGCTGGAGATCCACAGGAGGGTACTGGGGTATGTGGATCCAG TGGAGGCGGGCAGGTTCCGGAGGACCCAGGTCCTGGTGGGCCACCACATCCCACCCCACCCCCGGGATGT GGAGAAGCAGATGCAGGAGTTCACACAGTGGCTCAATTCAGAGGACGCCATGAACCTGCACCCAGTCGAG TTCGCGGCCCTGGCCCATTACAAACTGGTGTACATCCACCCTTTCATCGACGGCAACGGGAGGACCTCCC GTCTGCTGATGAACCTGATTTTGATGCAGGCGGGATACCCGCCCATCACCATCCGCAAGGAGCAGAGGTC CGAGTACTACCATGTACTGGAAGTCGCCAACGAGGGTGACGTGCGGCCTTTCATCCGCTTCATAGCCAAG TGTACGGAGGTCACACTGGACACGTTGCTCCTTGCCACCACCGAGTACTCGGTGGCACTGCCAGAAGCCC AGCCCAACCATTCTGGGTTCAAGGAGACGCTCCCTGTGAGGCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG216189 representing NM_001010825
Red=Cloning site Green=Tags(s) MILMPMASVVAVAEPKWVSVWGRFLWMALLSMALGSLLALLLPLGVVEEHCLAVLRGFHLLRSKLDRAQP VVPKCTSLCTELSVSSRDAGLLTVKTTASPAGKLEAKAALNQALEMKRQGKRGKAHKLFLHALKMDPGFV DALNEFGIFSEEDKDIIQADYLYTRALTISPFHEKALVNRDRTLPLVEEIDQRYFSVIDSKVKKVMSIPK GSSALRRVMEETYYHHIYHTVAIEGNTLTLSEIRHILETRYAVPGKSLEEQNEVIGMHAAMKYINTTLVS RIGSVTMDDMLEIHRRVLGYVDPVEAGRFRRTQVLVGHHIPPHPRDVEKQMQEFTQWLNSEDAMNLHPVE FAALAHYKLVYIHPFIDGNGRTSRLLMNLILMQAGYPPITIRKEQRSEYYHVLEVANEGDVRPFIRFIAK CTEVTLDTLLLATTEYSVALPEAQPNHSGFKETLPVRP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001010825 |
| ORF Size | 1374 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001010825.3, NP_001010825.2 |
| RefSeq Size | 3192 bp |
| RefSeq ORF | 1377 bp |
| Locus ID | 231630 |
| UniProt ID | Q8BIX9 |
| Gene Summary | Protein that can both mediate the addition of adenosine 5'-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context (By similarity). The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place (By similarity). Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of HSPA5/BiP (By similarity). In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP at 'Thr-518', thereby inactivating it (By similarity). In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from HSPA5/BiP at 'Thr-518', leading to restore HSPA5/BiP activity (By similarity). Although it is able to AMPylate RhoA, Rac and Cdc42 Rho GTPases in vitro, Rho GTPases do not constitute physiological substrates (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216196 | Ficd (untagged) - Mouse FIC domain containing (Ficd), (10ug) |
CNY 5,990.00 |
|
| MR216189 | Ficd (Myc-DDK-tagged) - Mouse FIC domain containing (Ficd) |
CNY 3,656.00 |
|
| MR216189L3 | Lenti ORF clone of Ficd (Myc-DDK-tagged) - Mouse FIC domain containing (Ficd) |
CNY 5,230.00 |
|
| MR216189L4 | Lenti ORF clone of Ficd (mGFP-tagged) - Mouse FIC domain containing (Ficd) |
CNY 5,230.00 |
