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Vps52 (NM_172620) Mouse Tagged ORF Clone
CAT＃: MG215732
- TrueORF®
Vps52 (tGFP-tagged) - Mouse vacuolar protein sorting 52 (yeast) (Vps52), (10ug)
ORF Plasmid: DDK
"NM_172620" in other vectors (4)
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CNY 6,370.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | ARE1; D130068D18; D430041K17Rik; SAC2; Sacm2l; tclw5 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG215732 representing NM_172620
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCCGCAGCGACCATGGCGGCCGCTGCCCGGGAGCTGGTGTTGCGGGCTGGGGCCTCAGATGTAG AGGAGGAGGAGGGCCCGCTGGGGGGGGGTTCTGGACTCCAAGAACCCCTACAACTTGGAGAGTTGGACAT CACCTCTGATGAATTCATCTTGGATGAAGTGGATGTTCACATCCAGGCAAACCTGGAGGATGAGTTAGTG AAGGAAGCTCTCAAAACGGGTGTGGATCTTCGACACTATTCAAAGCAGGTGGAGCTGGAGCTGCAGCAGA TTGAGCAGAAGTCAATCCGGGACTATATCCAAGAGAGTGAAAACATAGCGTCTCTGCACAATCAGATCAC GGCCTGTGACGCCGTCCTGGAGCGCATGGAACAGATGCTGGGAGCTTTTCAGAGCGATCTCAGCTCCATC AGCTCTGAGATCCGAACCCTGCAGGAGCAGTCGGGGGCCATGAACATCCGACTTCGTAACCGACAGGCAG TTCGGGGGAAACTTGGGGAACTTGTGGATGGGCTGGTGGTGCCCTCTGCTCTGGTCACAGCAATTCTGGA AGCTCCAGTGACCGAGCCCAGGTTCCTGGAGCAGCTACAGGAGCTGGATGCCAAGGCAGCCGCGGTGAGA GAGCAGGAGGCTATGGGCACCGCTGCCTGTGCTGACGTCAGAGGCGTGCTGGACCGGCTCCGGGTCAAGG CAGTGACGAAGATCCGGGAGTTTATTCTCCAGAAGATCTACTCCTTCAGAAAGCCCATGACCAACTACCA GATCCCCCAGGCGGCCCTGCTGAAGTACAGGTTTTTCTATCAGTTCCTGCTGGGTAATGAGCGTGCTACA GCCAAGGAGATCAGGGATGAGTACGTAGAGACGCTGAGCAAGATCTACCTGTCCTACTACCGATCCTATG TGGGGCGGCTCATGAAAGTGCAGTACGAGGAAGTTGCTGAGAAAGACGACCTAATGGGTGTTGAAGACAC AGCAAAGAAAGGCTTCTTCTCGAAGCCGTCCCTCCGAAGCAGGAACACCATCTTTACCCTTGGCACTCGT GGTACTGTCATCTCACCGGCCGAGCTGGAGGCCCCCATCCTAGTGCCCCATACTGCCCAACGTGGAGAGC AGAGGTATCCATTCGAAGCGCTCTTCCGCAGCCAGCACTATGCCCTCCTCGACAATTCTTGCCGTGAATA TCTTTTCATCTGTGAATTTTTCATCGTGTCTGGCCCGGCTGCACATGACCTGTTCCACGCCGTCATGGGC CGCACGCTCTCCATGACGCTGAAACACCTGGAGTCCTACCTGGCTGACTGCTACGATGCCATTGCTGTTT TTCTCTGTATCCACATTGTTCTCCGGTTCCGCAACATTGCAGCGAAGAGGGACGTCCCTGCCCTGGACAG GTACTGGGAGCAGGTGCTTGCCTTGCTGTGGCCTCGGTTTGAGCTGATCCTGGAGATGAATGTCCAGAGT GTCCGCAGCACTGACCCCCAGCGCCTTGGGGGACTGGACACTCGGCCCCACTATATCACACGCCGCTATG CTGAGTTCTCCTCTGCACTTGTCAGCATCAACCAGACGATCCCCAATGAACGCACGCTGCAGCTCCTGGG ACAGCTCCAGGTGGAGGTGGAGAATTTTGTCCTCCGAGTGGCTGCAGAGTTCTCCTCCAGGAAGGAGCAG CTTGTGTTTCTGATCAACAACTATGACATGATGCTCGGGGTGCTGATGGAGCGGGCTGCTGATGACAGCA AAGAGGTGGAAAGTTTCCAGCAGCTGCTCAATGCTCGGACACAGGAGTTCATTGAGGAGCTGCTGTCTCC CCCCTTCGGGGGTCTGGTGGCATTCGTGAAGGAGGCTGAAGCCTTGATTGAGCGTGGGCAGGCTGAGCGG CTCCGAGGGGAGGAAGCCCGAGTCACTCAGCTGATCCGTGGCTTTGGTAGTTCCTGGAAGGCATCAGTGG AGTCCCTGAGTCAGGATGTAATGCGAAGTTTTACCAACTTCCGAAATGGAACCAGCATCATCCAGGGGGC GCTGACCCAGCTGATCCAGCTCTACCATCGCTTCCACCGGGTGCTGTCTCAGCCCCAGCTCCGCGCACTC CCGGCCAGGGCCGAGCTCATCAACATCCATCACCTCATGGTGGAGCTGAAGAAACACAAGCCGAACTTC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG215732 representing NM_172620
Red=Cloning site Green=Tags(s) MAAAATMAAAARELVLRAGASDVEEEEGPLGGGSGLQEPLQLGELDITSDEFILDEVDVHIQANLEDELV KEALKTGVDLRHYSKQVELELQQIEQKSIRDYIQESENIASLHNQITACDAVLERMEQMLGAFQSDLSSI SSEIRTLQEQSGAMNIRLRNRQAVRGKLGELVDGLVVPSALVTAILEAPVTEPRFLEQLQELDAKAAAVR EQEAMGTAACADVRGVLDRLRVKAVTKIREFILQKIYSFRKPMTNYQIPQAALLKYRFFYQFLLGNERAT AKEIRDEYVETLSKIYLSYYRSYVGRLMKVQYEEVAEKDDLMGVEDTAKKGFFSKPSLRSRNTIFTLGTR GTVISPAELEAPILVPHTAQRGEQRYPFEALFRSQHYALLDNSCREYLFICEFFIVSGPAAHDLFHAVMG RTLSMTLKHLESYLADCYDAIAVFLCIHIVLRFRNIAAKRDVPALDRYWEQVLALLWPRFELILEMNVQS VRSTDPQRLGGLDTRPHYITRRYAEFSSALVSINQTIPNERTLQLLGQLQVEVENFVLRVAAEFSSRKEQ LVFLINNYDMMLGVLMERAADDSKEVESFQQLLNARTQEFIEELLSPPFGGLVAFVKEAEALIERGQAER LRGEEARVTQLIRGFGSSWKASVESLSQDVMRSFTNFRNGTSIIQGALTQLIQLYHRFHRVLSQPQLRAL PARAELINIHHLMVELKKHKPNF TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_172620 |
| ORF Size | 2169 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_172620.4 |
| RefSeq Size | 2766 bp |
| RefSeq ORF | 2172 bp |
| Locus ID | 224705 |
| UniProt ID | Q8C754 |
| Gene Summary | Acts as component of the GARP complex that is involved in retrograde transport from early and late endosomes to the trans-Golgi network (TGN). The GARP complex is required for the maintenance of the cycling of mannose 6-phosphate receptors between the TGN and endosomes, this cycling is necessary for proper lysosomal sorting of acid hydrolases such as CTSD. Acts as component of the EARP complex that is involved in endocytic recycling. The EARP complex associates with Rab4-positive endosomes and promotes recycling of internalized transferrin receptor (TFRC) to the plasma membrane.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221127 | Vps52 (untagged) - Mouse vacuolar protein sorting 52 (yeast) (Vps52), (10ug) |
CNY 9,310.00 |
|
| MR215732 | Vps52 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 52 (yeast) (Vps52) |
CNY 5,296.00 |
|
| MR215732L3 | Lenti ORF clone of Vps52 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 52 (yeast) (Vps52) |
CNY 7,700.00 |
|
| MR215732L4 | Lenti ORF clone of Vps52 (mGFP-tagged) - Mouse vacuolar protein sorting 52 (yeast) (Vps52) |
CNY 7,700.00 |
