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Pik3c2a (NM_011083) Mouse Tagged ORF Clone
CAT＃: MG215684
- TrueORF®
Pik3c2a (tGFP-tagged) - Mouse phosphatidylinositol 3-kinase C2 domain containing alpha polypeptide (Pik3c2a), (10ug)
ORF Plasmid: DDK
"NM_011083" in other vectors (2)
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CNY 15,580.00
货期*
9周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Cpk-m; PI3KC2 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG215684 representing NM_011083
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGGCGCGCCC ATGGCTCAGATTTCCAACAACAGTGAATTTAAACAATGTTCATCTTCACATCCAGAACCAATAAGAACCA AAGATGTGAACAAAGCAGAAGCGTTACAGATGGAAGCAGAAGCCTTAGCAAAACTGCAGAAGGATAGACA AATGACTGACAGCCCAAGAGGCTTTGAGCTGTCTAGCAGCACTAGACAAAGAACACAAGGTTTTAACAAA CAGGATTATGATCTCATGGTGTTTCCTGAGTTGGATTCCCAAAAAAGAGCAGTAGATATTGATGTAGAAA AGCTCACCCAGGCTGAACTTGAGAAGATATTGCTGGACGACAATTTTGAAACTAGAAAACCTCCTGCATT GCCAGTTACTCCTGTTCTGAGCCCTTCGTTCTCAACACAGCTGTATCTTAGACCTAGTGGTCAAAGAGGC CAGTGGCCCCCTGGATTATGCGGGCCTTCCACGTACACTTTACCTTCTACTTATCCTTCAGCATACAGTA AACAGGCCACATTCCAGAATGGCTTCAGTCCAAGGATGCCCACTTTTCCATCAACAGAGTCTGTATATTT AAGACTTCCTGGACAGTCTCCATATTTTTCATATCCTTTGACACCTGCCACACCATTTCATCCACAAGGA AGTTTACCAGTCTATCGGCCACTAGTCAGTCCTGACATGGCAAAACTATTTGAAAAAATAGCAAGTACCT CAGAATTTTTAAAAAATGGGAAAGCAAGGACTGATTTGGAGATAGCAAACTCGAAAGCTTCAGTCTGCAA TCTACAGATATCTCCAAAGTCTGAAGACATCAATAAGTTTGATTGGTTAGACTTGGATCCTCTGAGTAAG CCTAAGGTAGACTATGTGGAGGTGTTAGAACATGAAGAAGAGAAGAAGGATCCAGTTTTGCTAGCAGAGG ATCCTTGGGATGCTGTTCTTCTTGAAGAGAGATCGCCAAGTTGTCACCTAGAAAGAAAGGTGAATGGAAA ATCCCTTTCTGGGGCAACTGTAACAAGAAGCCAGTCTTTAATCATTCGGACAGCTCAATTTACAAAAGCC CAGGGCCAAGTATCTCAGAAAGACCCAAATGGGACCAGTAGTTTGCCAACTGGAAGTTCTCTTCTACAAG AATTTGAAGTACAGAATGACGAGGTGGCAGCTTTTTGTCAATCCATTATGAAATTGAAGACCAAATTTCC ATATACTGATCACTGCACAAATCCAGGCTATTTGTTAAGTCCAGTGACAGTGCAAAGAAACATGTGTGGG GAGAATGCCAGTGTGAAGGTCTCCATTGAAATTGAAGGGCTTCAACTACCAGTTACTTTTACATGTGATG TGAGTTCTACTGTAGAAATAATTATAATGCAAGCCCTTTGCTGGGTACATGATGACTTGAATCAAGTGGA TGTTGGCAGCTACATTCTGAAAGTTTGTGGTCAAGAGGAGGTTCTACAGAATAATCATTGCCTTGGAAGT CACGAACATATTCAAAATTGTCGAAAATGGGACACAGAGATTAAATTACAGCTCTTGACCTTGAGTGCAA TGTGCCAGAATCTGGCTCGAACAGCAGAAGATGATGAAGCACCTGTGGATTTAAACAAATACTTGTATCA AATAGAAAAACCTTATAAAGAAGTCATGACAAGACACCCTGTTGAAGAGCTCTTAGATTCCTATCACTAC CAAGTAGAACTGGCTCTTCAAACTGAAAACCAGCACCGAGCTGTTGATCAAGTGATTAAAGCAGTAAGAA AAATTTGTAGTGCTTTAGATGGGGTGGAGACCCCCTCCGTTACAGAAGCAGTGAAGAAGTTAAAGCGAGC AGTTAACCTTCCAAGGAATAAAAGTGCTGATGTGACTTCATTATCTGGAAGTGACACAAGGAAGAACTCA ACTAAGGGGTCACTGAATCCTGAAAATCCTGTTCAAGTAAGCATGGATCACCTAACAACAGCGATTTATG ATCTTCTCAGGCTCCATGCAAATTCTAGTAGGTGTTCTACAGGCTGTCCCCGAGGGAGCAGGAACATCAA GGAAGCATGGACTGCAACGGAGCAGCTCCAGTTCACTGTCTATGCCGCACACGGAATTTCCAGTAACTGG GTATCAAATTATGAAAAATACTACTTGATATGTTCCCTGTCTCACAATGGGAAGGATCTTTTTAAGCCTA TTCAGTCAAAGAAGGTTGGCACGTACAAGAATTTCTTCTATCTTATTAAATGGGATGAACTAATCATTTT TCCTATCCAGATATCGCAGTTGCCATTAGAATCAGTTCTTCATCTTACTCTGTTTGGAGTTTTAAATCAG AGCAGTGGAAGTTCCCCTGATTCTAATAAACAGAGAAAGGGGCCAGAAGCTCTGGGCAAAGTTTCTTTAA CTCTATTTGATTTTAAACGGTTTTTAACATGTGGAACTAAACTTCTCTACCTTTGGACTTCATCACATAC AAATTCTATTCCTGGAGCAATCCCCAAAAAAAGCTATGTCATGGAAAGAATTGTGCTACAGGTTGATTTT CCTTCTCCTGCGTTTGACATTATTTATACATCTCCTCAAATTGATAGAAACATTATACAGCAAGACAAGT TGGAAACACTGGAGAGTGATATAAAGGGGAAACTTCTGGATATTATTCACAGAGATTCATCATTTGGACT TTCTAAAGAAGATAAGGTCTTTTTGTGGGAAAACCGCTATTATTGCCTAAAACATCCAAATTGTCTTCCG AAGATATTAGCAAGTGCTCCAAACTGGAAGTGGGCTAATCTTGCCAAAACTTACTCATTGCTGCATCAGT GGCCGCCATTGTGCCCACTAGCTGCATTGGAGCTCCTTGATGCAAAATTTGCTGATCAGGAGGTGCGATC GCTTGCTGTGAGCTGGATGGAGGCCATTAGTGATGATGAGCTAGCAGATCTGCTCCCACAGTTCGTACAG GCTTTGAAATATGAAATTTATTTGAATAGTTCACTAGTGCGCTTCCTTCTGTCCAGGGCATTGGGAAACA TCCAGATAGCACACAGTTTGTATTGGCTTCTCAAGGATGCTTTGCATGATACACACTTTGGAAGCAGATA TGAACATGTGTTGGGTGCTCTCCTCTCTGTAGGAGGAAAAGGACTCAGAGAAGAGCTTTCTAAGCAGATG AAACTTGTACAGCTTTTAGGAGGAGTGGCAGAAAAAGTAAGGCAGGCTAGTGGATCAACAAGACAGGTTG TCCTCCAAAAGAGTATGGAACGGGTACAGTCCTTTTTTCTGAGAAATAAATGCCGTCTTCCTCTCAAACC AAGTCTAGTGGCAAAAGAACTAAATATTAAGTCATGTTCGTTCTTCAGTTCTAATGCTATGCCTCTGAAA GTCACAATGGTGAATGCTGACCCTCTGGGGGAAGAAATTAATGTCATGTTTAAGGTTGGTGAAGATCTTC GGCAAGATATGTTAGCTTTACAGATGATAAAGATTATGGATAAGATCTGGCTTAAAGAGGGACTGGATCT GAGGATGGTGATATTCAGATGCCTGTCAACTGGCCGAGATCGAGGCATGGTGGAGCTAGTTCCTGCTTCA GATACCCTCAGGAAAATCCAAGTGGAATATGGTGTAACAGGATCCTTTAAAGATAAACCACTTGCTGAGT GGCTGAGGAAATACAATCCTTCTGAAGAAGAATATGAAAAGGCTTCTGAGAACTTTATCTACTCTTGTGC TGGGTGCTGTGTAGCCACCTATGTTTTAGGCATTTGTGATCGGCACAATGACAATATAATGCTTCGAAGC ACAGGACACATGTTCCACATTGACTTTGGAAAGTTTTTGGGCCATGCACAGATGTTTGGTAGCTTCAAAA GGGACCGAGCTCCTTTTGTGCTTACCTCTGACATGGCGTATGTCATTAATGGAGGTGAAAAGCCCACCAT TCGTTTCCAGTTGTTTGTGGACCTCTGCTGTCAAGCCTACAACTTGATAAGAAAGCAAACAAACCTCTTT CTTAACCTTCTCTCACTGATGATTCCTTCAGGATTGCCAGAACTCACAAGTATTCAGGATCTGAAATATG TTAGAGATGCACTTCAGCCCCAAACTACAGATGCTGAAGCTACTATTTTCTTTACTAGGCTGATTGAGTC AAGTTTGGGAAGCATTGCCACAAAGTTTAATTTCTTCATTCATAACCTTGCTCAGCTACGTTTTTCTGGC CTTCCTTCTAATGATGAGCCCATCCTTTCATTCTCACCGAAAACATACTCCTTTAGACAAGATGGCCGGA TCAAGGAAGTCTCTGTTTTCACATATCATAAGAAATACAACCCAGATAAACACTATATTTATGTGGTTCG AATTCTAAGAGAAGGACACCTTGAACCATCATTTGTATTCCGGACATTTGATGAATTTCAGGAACTTCAC AATAAGCTCAGTATTATTTTTCCTCTTTGGAAATTACCTGGCTTTCCTAATAGGATGGTTCTTGGAAGAA CACACATAAAAGATGTTGCAGCCAAGAGGAAAATTGAATTAAACAGTTATTTACAGAGTTTGATGAATGC ATCAACAGATGTAGCAGAGTGTGATCTTGTTTGTACTTTTTTCCACCCTTTACTTCGTGATGAGAAAGCT GAAGGAATAGCTAGGTCTGCAGGTGCAGTTCCCTTCAGCCCAACTCTGGGCCAAATAGGAGGAGCAGTGA AGTTATCTGTTTCTTACCGAAATGGCACCCTCTTCATCATGGTGATGCACATCAAAGATCTTGTGACTGA AGATGGGGCTGACCCAAATCCCTATGTCAAAACATACCTGCTTCCAGATACCCACAAAACGTCAAAACGT AAAACCAAAATTTCACGTAAAACTAGGAACCCAACATTCAATGAAATGCTTGTATATAGTGGATACAGCA AAGAAACTCTGAGGCAGAGAGAACTTCAACTGAGTGTACTCAGTGCAGAATCACTGCGGGAGAATTTCTT CTTGGGTGGAATAACCCTGCCACTGAAAGATTTCAACTTGAGCAAAGAGACAGTTAAGTGGTATCAGCTG ACTGCGGCAACGTATCTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG215684 representing NM_011083
Red=Cloning site Green=Tags(s) MAQISNNSEFKQCSSSHPEPIRTKDVNKAEALQMEAEALAKLQKDRQMTDSPRGFELSSSTRQRTQGFNK QDYDLMVFPELDSQKRAVDIDVEKLTQAELEKILLDDNFETRKPPALPVTPVLSPSFSTQLYLRPSGQRG QWPPGLCGPSTYTLPSTYPSAYSKQATFQNGFSPRMPTFPSTESVYLRLPGQSPYFSYPLTPATPFHPQG SLPVYRPLVSPDMAKLFEKIASTSEFLKNGKARTDLEIANSKASVCNLQISPKSEDINKFDWLDLDPLSK PKVDYVEVLEHEEEKKDPVLLAEDPWDAVLLEERSPSCHLERKVNGKSLSGATVTRSQSLIIRTAQFTKA QGQVSQKDPNGTSSLPTGSSLLQEFEVQNDEVAAFCQSIMKLKTKFPYTDHCTNPGYLLSPVTVQRNMCG ENASVKVSIEIEGLQLPVTFTCDVSSTVEIIIMQALCWVHDDLNQVDVGSYILKVCGQEEVLQNNHCLGS HEHIQNCRKWDTEIKLQLLTLSAMCQNLARTAEDDEAPVDLNKYLYQIEKPYKEVMTRHPVEELLDSYHY QVELALQTENQHRAVDQVIKAVRKICSALDGVETPSVTEAVKKLKRAVNLPRNKSADVTSLSGSDTRKNS TKGSLNPENPVQVSMDHLTTAIYDLLRLHANSSRCSTGCPRGSRNIKEAWTATEQLQFTVYAAHGISSNW VSNYEKYYLICSLSHNGKDLFKPIQSKKVGTYKNFFYLIKWDELIIFPIQISQLPLESVLHLTLFGVLNQ SSGSSPDSNKQRKGPEALGKVSLTLFDFKRFLTCGTKLLYLWTSSHTNSIPGAIPKKSYVMERIVLQVDF PSPAFDIIYTSPQIDRNIIQQDKLETLESDIKGKLLDIIHRDSSFGLSKEDKVFLWENRYYCLKHPNCLP KILASAPNWKWANLAKTYSLLHQWPPLCPLAALELLDAKFADQEVRSLAVSWMEAISDDELADLLPQFVQ ALKYEIYLNSSLVRFLLSRALGNIQIAHSLYWLLKDALHDTHFGSRYEHVLGALLSVGGKGLREELSKQM KLVQLLGGVAEKVRQASGSTRQVVLQKSMERVQSFFLRNKCRLPLKPSLVAKELNIKSCSFFSSNAMPLK VTMVNADPLGEEINVMFKVGEDLRQDMLALQMIKIMDKIWLKEGLDLRMVIFRCLSTGRDRGMVELVPAS DTLRKIQVEYGVTGSFKDKPLAEWLRKYNPSEEEYEKASENFIYSCAGCCVATYVLGICDRHNDNIMLRS TGHMFHIDFGKFLGHAQMFGSFKRDRAPFVLTSDMAYVINGGEKPTIRFQLFVDLCCQAYNLIRKQTNLF LNLLSLMIPSGLPELTSIQDLKYVRDALQPQTTDAEATIFFTRLIESSLGSIATKFNFFIHNLAQLRFSG LPSNDEPILSFSPKTYSFRQDGRIKEVSVFTYHKKYNPDKHYIYVVRILREGHLEPSFVFRTFDEFQELH NKLSIIFPLWKLPGFPNRMVLGRTHIKDVAAKRKIELNSYLQSLMNASTDVAECDLVCTFFHPLLRDEKA EGIARSAGAVPFSPTLGQIGGAVKLSVSYRNGTLFIMVMHIKDLVTEDGADPNPYVKTYLLPDTHKTSKR KTKISRKTRNPTFNEMLVYSGYSKETLRQRELQLSVLSAESLRENFFLGGITLPLKDFNLSKETVKWYQL TAATYL TRTRPLE - GFP Tag - V |
| Restriction Sites |
AscI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011083 |
| ORF Size | 5058 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011083.2, NP_035213.2 |
| RefSeq Size | 8042 bp |
| RefSeq ORF | 5061 bp |
| Locus ID | 18704 |
| UniProt ID | Q61194 |
| Gene Summary | Generates phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) that act as second messengers. Has a role in several intracellular trafficking events. Functions in insulin signaling and secretion. Required for translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane and glucose uptake in response to insulin-mediated RHOQ activation. Regulates insulin secretion through two different mechanisms: involved in glucose-induced insulin secretion downstream of insulin receptor in a pathway that involves AKT1 activation and TBC1D4/AS160 phosphorylation, and participates in the late step of insulin granule exocytosis probably in insulin granule fusion. Synthesizes PtdIns3P in response to insulin signaling. Functions in clathrin-coated endocytic vesicle formation and distribution. Regulates dynamin-independent endocytosis, probably by recruiting EEA1 to internalizing vesicles. In neurosecretory cells synthesizes PtdIns3P on large dense core vesicles. Participates in calcium induced contraction of vascular smooth muscle by regulating myosin light chain (MLC) phosphorylation through a mechanism involving Rho kinase-dependent phosphorylation of the MLCP-regulatory subunit MYPT1. May play a role in the EGF signaling cascade. May be involved in mitosis and UV-induced damage response. Required for maintenance of normal renal structure and function by supporting normal podocyte function.[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224824 | Pik3c2a (untagged) - Mouse phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide (Pik3c2a), (10ug) |
CNY 27,080.00 |
|
| MR215684 | Pik3c2a (Myc-DDK-tagged) - Mouse phosphatidylinositol 3-kinase, C2 domain containing, alpha polypeptide (Pik3c2a) |
CNY 11,336.00 |
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