Pik3c2a (NM_011083) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Cpk-m; PI3KC2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC224824 representing NM_011083 Red=Cloning site Blue=ORF Orange=Stop codon CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCCGGCGC GCCC ATGGCTCAGATTTCCAACAACAGTGAATTTAAACAATGTTCATCTTCACATCCAGAACCAATAAGAACCA AAGATGTGAACAAAGCAGAAGCGTTACAGATGGAAGCAGAAGCCTTAGCAAAACTGCAGAAGGATAGACA AATGACTGACAGCCCAAGAGGCTTTGAGCTGTCTAGCAGCACTAGACAAAGAACACAAGGTTTTAACAAA CAGGATTATGATCTCATGGTGTTTCCTGAGTTGGATTCCCAAAAAAGAGCAGTAGATATTGATGTAGAAA AGCTCACCCAGGCTGAACTTGAGAAGATATTGCTGGACGACAATTTTGAAACTAGAAAACCTCCTGCATT GCCAGTTACTCCTGTTCTGAGCCCTTCGTTCTCAACACAGCTGTATCTTAGACCTAGTGGTCAAAGAGGC CAGTGGCCCCCTGGATTATGCGGGCCTTCCACGTACACTTTACCTTCTACTTATCCTTCAGCATACAGTA AACAGGCCACATTCCAGAATGGCTTCAGTCCAAGGATGCCCACTTTTCCATCAACAGAGTCTGTATATTT AAGACTTCCTGGACAGTCTCCATATTTTTCATATCCTTTGACACCTGCCACACCATTTCATCCACAAGGA AGTTTACCAGTCTATCGGCCACTAGTCAGTCCTGACATGGCAAAACTATTTGAAAAAATAGCAAGTACCT CAGAATTTTTAAAAAATGGGAAAGCAAGGACTGATTTGGAGATAGCAAACTCGAAAGCTTCAGTCTGCAA TCTACAGATATCTCCAAAGTCTGAAGACATCAATAAGTTTGATTGGTTAGACTTGGATCCTCTGAGTAAG CCTAAGGTAGACTATGTGGAGGTGTTAGAACATGAAGAAGAGAAGAAGGATCCAGTTTTGCTAGCAGAGG ATCCTTGGGATGCTGTTCTTCTTGAAGAGAGATCGCCAAGTTGTCACCTAGAAAGAAAGGTGAATGGAAA ATCCCTTTCTGGGGCAACTGTAACAAGAAGCCAGTCTTTAATCATTCGGACAGCTCAATTTACAAAAGCC CAGGGCCAAGTATCTCAGAAAGACCCAAATGGGACCAGTAGTTTGCCAACTGGAAGTTCTCTTCTACAAG AATTTGAAGTACAGAATGACGAGGTGGCAGCTTTTTGTCAATCCATTATGAAATTGAAGACCAAATTTCC ATATACTGATCACTGCACAAATCCAGGCTATTTGTTAAGTCCAGTGACAGTGCAAAGAAACATGTGTGGG GAGAATGCCAGTGTGAAGGTCTCCATTGAAATTGAAGGGCTTCAACTACCAGTTACTTTTACATGTGATG TGAGTTCTACTGTAGAAATAATTATAATGCAAGCCCTTTGCTGGGTACATGATGACTTGAATCAAGTGGA TGTTGGCAGCTACATTCTGAAAGTTTGTGGTCAAGAGGAGGTTCTACAGAATAATCATTGCCTTGGAAGT CACGAACATATTCAAAATTGTCGAAAATGGGACACAGAGATTAAATTACAGCTCTTGACCTTGAGTGCAA TGTGCCAGAATCTGGCTCGAACAGCAGAAGATGATGAAGCACCTGTGGATTTAAACAAATACTTGTATCA AATAGAAAAACCTTATAAAGAAGTCATGACAAGACACCCTGTTGAAGAGCTCTTAGATTCCTATCACTAC CAAGTAGAACTGGCTCTTCAAACTGAAAACCAGCACCGAGCTGTTGATCAAGTGATTAAAGCAGTAAGAA AAATTTGTAGTGCTTTAGATGGGGTGGAGACCCCCTCCGTTACAGAAGCAGTGAAGAAGTTAAAGCGAGC AGTTAACCTTCCAAGGAATAAAAGTGCTGATGTGACTTCATTATCTGGAAGTGACACAAGGAAGAACTCA ACTAAGGGGTCACTGAATCCTGAAAATCCTGTTCAAGTAAGCATGGATCACCTAACAACAGCGATTTATG ATCTTCTCAGGCTCCATGCAAATTCTAGTAGGTGTTCTACAGGCTGTCCCCGAGGGAGCAGGAACATCAA GGAAGCATGGACTGCAACGGAGCAGCTCCAGTTCACTGTCTATGCCGCACACGGAATTTCCAGTAACTGG GTATCAAATTATGAAAAATACTACTTGATATGTTCCCTGTCTCACAATGGGAAGGATCTTTTTAAGCCTA TTCAGTCAAAGAAGGTTGGCACGTACAAGAATTTCTTCTATCTTATTAAATGGGATGAACTAATCATTTT TCCTATCCAGATATCGCAGTTGCCATTAGAATCAGTTCTTCATCTTACTCTGTTTGGAGTTTTAAATCAG AGCAGTGGAAGTTCCCCTGATTCTAATAAACAGAGAAAGGGGCCAGAAGCTCTGGGCAAAGTTTCTTTAA CTCTATTTGATTTTAAACGGTTTTTAACATGTGGAACTAAACTTCTCTACCTTTGGACTTCATCACATAC AAATTCTATTCCTGGAGCAATCCCCAAAAAAAGCTATGTCATGGAAAGAATTGTGCTACAGGTTGATTTT CCTTCTCCTGCGTTTGACATTATTTATACATCTCCTCAAATTGATAGAAACATTATACAGCAAGACAAGT TGGAAACACTGGAGAGTGATATAAAGGGGAAACTTCTGGATATTATTCACAGAGATTCATCATTTGGACT TTCTAAAGAAGATAAGGTCTTTTTGTGGGAAAACCGCTATTATTGCCTAAAACATCCAAATTGTCTTCCG AAGATATTAGCAAGTGCTCCAAACTGGAAGTGGGCTAATCTTGCCAAAACTTACTCATTGCTGCATCAGT GGCCGCCATTGTGCCCACTAGCTGCATTGGAGCTCCTTGATGCAAAATTTGCTGATCAGGAGGTGCGATC GCTTGCTGTGAGCTGGATGGAGGCCATTAGTGATGATGAGCTAGCAGATCTGCTCCCACAGTTCGTACAG GCTTTGAAATATGAAATTTATTTGAATAGTTCACTAGTGCGCTTCCTTCTGTCCAGGGCATTGGGAAACA TCCAGATAGCACACAGTTTGTATTGGCTTCTCAAGGATGCTTTGCATGATACACACTTTGGAAGCAGATA TGAACATGTGTTGGGTGCTCTCCTCTCTGTAGGAGGAAAAGGACTCAGAGAAGAGCTTTCTAAGCAGATG AAACTTGTACAGCTTTTAGGAGGAGTGGCAGAAAAAGTAAGGCAGGCTAGTGGATCAACAAGACAGGTTG TCCTCCAAAAGAGTATGGAACGGGTACAGTCCTTTTTTCTGAGAAATAAATGCCGTCTTCCTCTCAAACC AAGTCTAGTGGCAAAAGAACTAAATATTAAGTCATGTTCGTTCTTCAGTTCTAATGCTATGCCTCTGAAA GTCACAATGGTGAATGCTGACCCTCTGGGGGAAGAAATTAATGTCATGTTTAAGGTTGGTGAAGATCTTC GGCAAGATATGTTAGCTTTACAGATGATAAAGATTATGGATAAGATCTGGCTTAAAGAGGGACTGGATCT GAGGATGGTGATATTCAGATGCCTGTCAACTGGCCGAGATCGAGGCATGGTGGAGCTAGTTCCTGCTTCA GATACCCTCAGGAAAATCCAAGTGGAATATGGTGTAACAGGATCCTTTAAAGATAAACCACTTGCTGAGT GGCTGAGGAAATACAATCCTTCTGAAGAAGAATATGAAAAGGCTTCTGAGAACTTTATCTACTCTTGTGC TGGGTGCTGTGTAGCCACCTATGTTTTAGGCATTTGTGATCGGCACAATGACAATATAATGCTTCGAAGC ACAGGACACATGTTCCACATTGACTTTGGAAAGTTTTTGGGCCATGCACAGATGTTTGGTAGCTTCAAAA GGGACCGAGCTCCTTTTGTGCTTACCTCTGACATGGCGTATGTCATTAATGGAGGTGAAAAGCCCACCAT TCGTTTCCAGTTGTTTGTGGACCTCTGCTGTCAAGCCTACAACTTGATAAGAAAGCAAACAAACCTCTTT CTTAACCTTCTCTCACTGATGATTCCTTCAGGATTGCCAGAACTCACAAGTATTCAGGATCTGAAATATG TTAGAGATGCACTTCAGCCCCAAACTACAGATGCTGAAGCTACTATTTTCTTTACTAGGCTGATTGAGTC AAGTTTGGGAAGCATTGCCACAAAGTTTAATTTCTTCATTCATAACCTTGCTCAGCTACGTTTTTCTGGC CTTCCTTCTAATGATGAGCCCATCCTTTCATTCTCACCGAAAACATACTCCTTTAGACAAGATGGCCGGA TCAAGGAAGTCTCTGTTTTCACATATCATAAGAAATACAACCCAGATAAACACTATATTTATGTGGTTCG AATTCTAAGAGAAGGACACCTTGAACCATCATTTGTATTCCGGACATTTGATGAATTTCAGGAACTTCAC AATAAGCTCAGTATTATTTTTCCTCTTTGGAAATTACCTGGCTTTCCTAATAGGATGGTTCTTGGAAGAA CACACATAAAAGATGTTGCAGCCAAGAGGAAAATTGAATTAAACAGTTATTTACAGAGTTTGATGAATGC ATCAACAGATGTAGCAGAGTGTGATCTTGTTTGTACTTTTTTCCACCCTTTACTTCGTGATGAGAAAGCT GAAGGAATAGCTAGGTCTGCAGGTGCAGTTCCCTTCAGCCCAACTCTGGGCCAAATAGGAGGAGCAGTGA AGTTATCTGTTTCTTACCGAAATGGCACCCTCTTCATCATGGTGATGCACATCAAAGATCTTGTGACTGA AGATGGGGCTGACCCAAATCCCTATGTCAAAACATACCTGCTTCCAGATACCCACAAAACGTCAAAACGT AAAACCAAAATTTCACGTAAAACTAGGAACCCAACATTCAATGAAATGCTTGTATATAGTGGATACAGCA AAGAAACTCTGAGGCAGAGAGAACTTCAACTGAGTGTACTCAGTGCAGAATCACTGCGGGAGAATTTCTT CTTGGGTGGAATAACCCTGCCACTGAAAGATTTCAACTTGAGCAAAGAGACAGTTAAGTGGTATCAGCTG ACTGCGGCAACGTATCTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	AscI-MluI
ACCN	NM_011083
Insert Size	5061 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_011083.2, NP_035213.2
RefSeq Size	8042 bp
RefSeq ORF	5061 bp
Locus ID	18704
UniProt ID	Q61194
Gene Summary	Generates phosphatidylinositol 3-phosphate (PtdIns3P) and phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2) that act as second messengers. Has a role in several intracellular trafficking events. Functions in insulin signaling and secretion. Required for translocation of the glucose transporter SLC2A4/GLUT4 to the plasma membrane and glucose uptake in response to insulin-mediated RHOQ activation. Regulates insulin secretion through two different mechanisms: involved in glucose-induced insulin secretion downstream of insulin receptor in a pathway that involves AKT1 activation and TBC1D4/AS160 phosphorylation, and participates in the late step of insulin granule exocytosis probably in insulin granule fusion. Synthesizes PtdIns3P in response to insulin signaling. Functions in clathrin-coated endocytic vesicle formation and distribution. Regulates dynamin-independent endocytosis, probably by recruiting EEA1 to internalizing vesicles. In neurosecretory cells synthesizes PtdIns3P on large dense core vesicles. Participates in calcium induced contraction of vascular smooth muscle by regulating myosin light chain (MLC) phosphorylation through a mechanism involving Rho kinase-dependent phosphorylation of the MLCP-regulatory subunit MYPT1. May play a role in the EGF signaling cascade. May be involved in mitosis and UV-induced damage response. Required for maintenance of normal renal structure and function by supporting normal podocyte function.[UniProtKB/Swiss-Prot Function]

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