Ctsh (NM_007801) Mouse Tagged ORF Clone
CAT#: MG215191
- TrueORF®
Ctsh (tGFP-tagged) - Mouse cathepsin H (Ctsh), (10ug)
ORF Plasmid: DDK
"NM_007801" in other vectors (3)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AL022844 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG215191 representing NM_007801
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGGCTGCGCTGCCGCTGCTGTGCGCTGGGGCCTGGCTGCTGAGTACTGGGGCCACCGCCGAGCTGA CCGTGAACGCCATAGAAAAGTTTCACTTTAAGTCATGGATGAAACAGCATCAAAAGACGTACAGCTCGGT GGAGTACAACCACAGACTGCAGATGTTTGCCAACAACTGGAGGAAGATTCAAGCCCACAACCAGAGGAAC CACACATTTAAAATGGCATTGAACCAGTTTTCAGATATGAGCTTTGCTGAAATAAAACACAAATTCCTTT GGTCAGAGCCTCAGAATTGCTCAGCCACCAAAAGTAACTACCTCCGAGGTACAGGCCCCTACCCTTCCTC CATGGACTGGAGGAAGAAAGGAAATGTTGTTTCGCCAGTGATAAATCAGGGGGCCTGTGGCAGCTGCTGG ACTTTCTCTACCACGGGGGCCCTAGAGTCAGCTGTGGCTATTGCCAGTGGGAAAATGCTGTCTTTGGCTG AGCAGCAGCTGGTGGATTGTGCCCAAGCCTTCAACAATCATGGCTGCAAAGGAGGTCTCCCCAGCCAGGC CTTCGAGTACATCCTATACAACAAGGGCATCATGGAAGAAGACAGCTACCCTTACATAGGCAAGGATAGT TCATGCAGATTCAACCCCCAAAAAGCTGTTGCATTCGTCAAGAATGTTGTCAACATCACACTCAATGACG AGGCTGCAATGGTTGAGGCTGTGGCTCTATACAACCCTGTGAGCTTCGCCTTTGAGGTGACTGAAGATTT TTTGATGTATAAAAGTGGCGTCTACTCCAGTAAATCCTGTCATAAAACTCCAGATAAAGTAAACCATGCA GTCCTGGCGGTTGGCTATGGAGAACAGAATGGATTACTCTACTGGATTGTGAAAAACTCTTGGGGCTCCC AGTGGGGGGAGAATGGGTACTTCCTCATTGAACGTGGGAAGAACATGTGTGGCCTGGCTGCTTGTGCCTC CTATCCCATTCCTCAGGTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG215191 representing NM_007801
Red=Cloning site Green=Tags(s) MWAALPLLCAGAWLLSTGATAELTVNAIEKFHFKSWMKQHQKTYSSVEYNHRLQMFANNWRKIQAHNQRN HTFKMALNQFSDMSFAEIKHKFLWSEPQNCSATKSNYLRGTGPYPSSMDWRKKGNVVSPVINQGACGSCW TFSTTGALESAVAIASGKMLSLAEQQLVDCAQAFNNHGCKGGLPSQAFEYILYNKGIMEEDSYPYIGKDS SCRFNPQKAVAFVKNVVNITLNDEAAMVEAVALYNPVSFAFEVTEDFLMYKSGVYSSKSCHKTPDKVNHA VLAVGYGEQNGLLYWIVKNSWGSQWGENGYFLIERGKNMCGLAACASYPIPQV TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007801 |
ORF Size | 999 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007801.2, NP_031827.2 |
RefSeq Size | 1627 bp |
RefSeq ORF | 1002 bp |
Locus ID | 13036 |
UniProt ID | P49935 |
Gene Summary | This gene encodes a member of the peptidase C1 (papain) family of cysteine proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate multiple protein products. These products include the cathepsin H mini, heavy, and light chains. In rat and human, these three chains can associate to form the mature enzyme, which has both aminopeptidase and endopeptidase activities. Homozygous knockout mice for this gene exhibit impaired lung surfactant processing and reduced tumorigenesis in a pancreatic cancer model. Multiple pseudogenes of this gene have been identified in the genome. [provided by RefSeq, Aug 2015] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR215191 | Ctsh (Myc-DDK-tagged) - Mouse cathepsin H (Ctsh) |
CNY 2,400.00 |
|
MR215191L3 | Lenti ORF clone of Ctsh (Myc-DDK-tagged) - Mouse cathepsin H (Ctsh) |
CNY 4,750.00 |
|
MR215191L4 | Lenti ORF clone of Ctsh (mGFP-tagged) - Mouse cathepsin H (Ctsh) |
CNY 4,750.00 |