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Safb (NM_001163300) Mouse Tagged ORF Clone
CAT＃: MG215176
- TrueORF®
Safb (tGFP-tagged) - Mouse scaffold attachment factor B (Safb), (10ug)
ORF Plasmid: DDK
"NM_001163300" in other vectors (4)
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CNY 9,120.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 3110021E02Rik; 5330423C17Rik; AU018122; D18386; E130307D12; HAP; HET; SAF-B1; SAFB1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG215176 representing NM_001163300
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGACTCTGTCGGGTTTAGGAGACGCGAGTGCGGCGGGCGCGGCGGCCGTGAGCTCCGCGGCGT CAGAGACCGGGACGCGGCGGCTCAGCGACCTGCGGGTGATCGACCTGCGGGCGGAGCTGAAGAAGCGGAA CCTCGACTCGAGCGGCAACAAGAGCGTGCTGATGGAGCGGCTGAAGAAGGCCATTGAAGATGAAGGTGGG AATCCTGATGAAATTGAAGTCACCTCAGAATGCAACAAGAAGATGCCTAAGAGGCCCAGCAAAGGGAGGA AACCAGAAGACGAGGGTGTGGAGGACAACGGGCTCGAGGAGAATTCTGGGGATGGGCAGGAGGATGTGGA GACCAGCCTGGAGAACCTGCAAGACATGGATATGATGGACATCAGTGTGCTGGATGAGGCTGACATTGAC AATGGCAGCGTGGCAGACTGTGTGGAGGAAGAGGAGGAGGCCACACTCCCTGAGGGCCTAGCAGACAGCA CTGAACTGGTAGAAGGAGATTTGAAAGGGCTCCCAGAACAGCTGCAGGAGCATGCTATAGATGACAAGGA CACCGTTAACAATGTAGATACGTCATCATCTGACTTCACCATGCTGCAGGAGATGGAAGAGGCATCTTTG GAGCCAGAAAATGAGAAAATACTCGACATTTTGGGGGAAACTTGTAAATCTGAGCCAGTAAAAGAAGAAG GTTCGGAGCTGGAGCAGCCCTTTGCCCAGGCCACGAGTAGCGTGGGGCCAGACAGGAAGCTGGCGGAGGA AGAGGACCTATTTGAGAGCTGCGGCCACCCGGAAGAGGAAGAGGAAGAGGAGGAAGAAGATCAGGAAGAG GAGCAGGAGGAGGAGGGAGATTTAGCTTTGGCCAGCAGCAGCAAGTCTGAGTCTCCAAGCACTCGGTGTC AGTGGAGCGAGGCAGATGCCCCGTTAGCAGTAGTGAAAAGGGAGCCGGCAGATGCGCCTGGCGGAGGCAC TGGGATGGACCGCGAGCCTGTAGGGCTAGAGGAGCCAGTTGAACAGAGTAGCACGGCTGCCCAGCTCCCG GAGGCCACCAGCCAGGAGCTGGTGCGAGCGCCCACGGCAGCCCTGAGCCCTGAGCCCCAAGATAGCAAAG AAGACGTGAAGAAGTTTGCTTTTGACGCTTGTAATGACGTCCCTGCGCCTCCTAAAGAGTCCTCAGCCAG TGAGGGCGCTGATCAGAAAATGAGTTCTGTGGAGGAGGACTCGGATACAAAAAGGCTTTCCCGAGAAGAG AAGGGTCGCAGCAGCTGTGGTAGGAATTTCTGGGTTAGTGGCCTTTCGTCTACAACCAGAGCCACGGATC TGAAGAACCTGTTTAGCAGATATGGGAAGGTGGTGGGTGCTAAGGTTGTGACGAACGCCCGGAGTCCTGG AGCTCGCTGTTATGGCTTTGTCACTATGTCCACAGCTGAAGAGGCAACAAAATGCATTAGCCATTTGCAC AAGACTGAGCTGCATGGCAAGATGATCTCAGTGGAAAAGGCCAAAAGTGAGCCCACTGGCAAAAGAGTGC CTGACAGGCGAGATGGGGACAGCAAGAAGGAGAAGGCCAGCACCAGTGACAGATCTGCAAACCTTAAGAG GGAGGAGAAAGGTGAGAGAAAAGACGATGCCAAGAAGACAGATGATGGGAGCACAGAGAAGAGCAAAGAC GCAGATGACCAGAAACCTGGGCCTTCAGAGCGCTCGCGAACGACCAAGTCAGGAAGCCGTGGCACTGAGC GCACTGTGGTGATGGACAAGTCCAAAGGTGTGCCTGTCATCAGTGTGAAAACATCTGGGTCTAAAGAGAG AGCCTCCAAAAGTCAGGACCGGAAGTCAGCCAGCCGGGAGAAGCGGTCTGTTGTGTCCTTTGACAAAGTT AAAGAGTCTCGAAAGTCCAGAGACTCAGAGTCTCGGAGGGAACGGGAGCGCAGTGAGCGGGAGCAGAGGC TGCAGGCCCAGTGGGAGCGGGAGGAGCGAGAGCGGCTGGAGATTGCTCGTGAGCGCTTGGCATTCCACCG CCATCGGCTAGAGCGTGAACGCATGGAGCGGGAGCGGCTGGAGCGGGAGCGGATGCATGTAGAACAGGAG CGGAGGCGTGAGCAAGAGCGCATTCACCGAGAGCGAGAGGAGCTTCGGCGTCAGCAGGAGCTGCGCTATG AACAGGAGCGCCGGCCTGCTGTGCGCAGGCCCTATGAAGTGGATGGCCGGCGAGATGATGCCTATTGGCC AGAAGCTAAACGGGCTGCTCTGGATGACCGTTACCACTCAGACTTCAGCCGCCAGGACCGCTTTCACGAC TTCGACCACAGAGACAGAGGCCGTTATCCCAACCATTCTGTGGATAGGAGAGAAGGTTCACGGTCGATGA TGGGAGACAGAGAAGGACAGCATTACCCTGAACGCCATGGAGGACCTGAACGCCATGGCCGGGACTCTCG AGATGGCTGGGGCTATGGTTCGAACAAGAGATTGAGTGAAGGCCGGGGGCTGCCCCCTCCTCCCAGGCGT GACTGGGGGGAGCATGGCCGGCGACTTGAAGATGACCGGGCATGGCAGGGCACGGCTGATGGAGGCATGA TGGAGCGTGATCATAAGAGGTGGCAAGGTGGTGAGAGGAGCATGTCTGGCCACTCGGGGCCTGGTCACAT GATGAACCGTGGTGGCATGTCAGGGCGAGGCAGTTTTGCCCCAGGAGGAGCTTCTCGTGGCCATGTGATC CCACGTGGAGGGATGCAGGCAGGCTTTGGAGGACAGAGCCGGGGAAGCAGACCCAGTGATGCCCGCTTCA CCCGCCGCTAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG215176 representing NM_001163300
Red=Cloning site Green=Tags(s) MAETLSGLGDASAAGAAAVSSAASETGTRRLSDLRVIDLRAELKKRNLDSSGNKSVLMERLKKAIEDEGG NPDEIEVTSECNKKMPKRPSKGRKPEDEGVEDNGLEENSGDGQEDVETSLENLQDMDMMDISVLDEADID NGSVADCVEEEEEATLPEGLADSTELVEGDLKGLPEQLQEHAIDDKDTVNNVDTSSSDFTMLQEMEEASL EPENEKILDILGETCKSEPVKEEGSELEQPFAQATSSVGPDRKLAEEEDLFESCGHPEEEEEEEEEDQEE EQEEEGDLALASSSKSESPSTRCQWSEADAPLAVVKREPADAPGGGTGMDREPVGLEEPVEQSSTAAQLP EATSQELVRAPTAALSPEPQDSKEDVKKFAFDACNDVPAPPKESSASEGADQKMSSVEEDSDTKRLSREE KGRSSCGRNFWVSGLSSTTRATDLKNLFSRYGKVVGAKVVTNARSPGARCYGFVTMSTAEEATKCISHLH KTELHGKMISVEKAKSEPTGKRVPDRRDGDSKKEKASTSDRSANLKREEKGERKDDAKKTDDGSTEKSKD ADDQKPGPSERSRTTKSGSRGTERTVVMDKSKGVPVISVKTSGSKERASKSQDRKSASREKRSVVSFDKV KESRKSRDSESRRERERSEREQRLQAQWEREERERLEIARERLAFHRHRLERERMERERLERERMHVEQE RRREQERIHREREELRRQQELRYEQERRPAVRRPYEVDGRRDDAYWPEAKRAALDDRYHSDFSRQDRFHD FDHRDRGRYPNHSVDRREGSRSMMGDREGQHYPERHGGPERHGRDSRDGWGYGSNKRLSEGRGLPPPPRR DWGEHGRRLEDDRAWQGTADGGMMERDHKRWQGGERSMSGHSGPGHMMNRGGMSGRGSFAPGGASRGHVI PRGGMQAGFGGQSRGSRPSDARFTRRY TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001163300 |
| ORF Size | 2811 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001163300.1, NP_001156772.1 |
| RefSeq Size | 3183 bp |
| RefSeq ORF | 2814 bp |
| Locus ID | 224903 |
| UniProt ID | D3YXK2 |
| Gene Summary | Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing. Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. Can inhibit cell proliferation. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222754 | Safb (untagged) - Mouse scaffold attachment factor B (Safb), (10ug) |
CNY 12,070.00 |
|
| MR215176 | Safb (Myc-DDK-tagged) - Mouse scaffold attachment factor B (Safb) |
CNY 6,864.00 |
|
| MR215176L3 | Lenti ORF clone of Safb (Myc-DDK-tagged) - Mouse scaffold attachment factor B (Safb) |
CNY 10,170.00 |
|
| MR215176L4 | Lenti ORF clone of Safb (mGFP-tagged) - Mouse scaffold attachment factor B (Safb) |
CNY 10,170.00 |
