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Smpdl3a (NM_020561) Mouse Tagged ORF Clone
CAT＃: MG214746
- TrueORF®
Smpdl3a (tGFP-tagged) - Mouse sphingomyelin phosphodiesterase acid-like 3A (Smpdl3a), (10ug)
ORF Plasmid: DDK
"NM_020561" in other vectors (4)
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CNY 3,710.00
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Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 0610010C24Rik; AI529588; ASM3A; ASML3; ASML3A |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG214746 representing NM_020561
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTTGCTGGGCAACTTCCTTTGCTGCCTACTGGTTGCCTGGCTCTGCGGCCCGGGGCTCGGGGTGC CCCTGGCGCCGGCGGATCGCGCCCCAGCTGTGGGGCAGTTTTGGCACGTGACTGACTTACATCTAGACCC TACTTACCACATTACAGATGACCGTACCAAGGTGTGTGCTTCATCTAAAGGCGCAAATGCCTCCAACCCT GGGCCTTTCGGAGATGTCCTGTGTGACTCTCCATATCAACTTATTTTGTCAGCCTTTGATTTTATTAAGA ATTCAGGACAAGAAGCATCTTTCATGATATGGACAGGGGATAGCCCACCTCATGTGCCAGTACCTGAACT CTCCACAGGCACCGTGATAAAGGTGATCACTAACATGACAATGACTGTCCAGAACCTGTTTCCAAACCTC CAGGTTTTCCCTGCACTGGGCAATCATGACTACTGGCCACAGGACCAGCTGCCAATAGTCACCAGTAAGG TCTACAGTGCTGTGGCTGACCTCTGGAAACCCTGGCTGGGTGAAGAAGCTATTAGCACTTTAAAGAAAGG TGGTTTTTACTCACAGAAAGTTGCAAGTAATCCAGGCTTGAGGATCATTAGCCTAAACACAAACTTGTAC TATGGCCCAAACATCATGACCCTGAACAAGACAGACCCAGCAAATCAGTTTGAATGGCTGGAAAATACAC TCAACAGCTCTCTATGGAATAAGGAGAAGGTATACATCATAGCGCATGTTCCAGTGGGCTATCTCCCTTA TGCAACTGACACCCCGGCGATAAGGCAGTACTATAATGAGAAACTGCTTGATATTTTCAGAAGATACAGC TCCGTCATTGCGGGACAGTTCTATGGCCACACCCATAGAGACAGCCTTATGGTCCTTTCAGATAAAAACG GGAATCCACTCAATTCTGTGTTTGTGGCACCTGCTGTTACACCAGTGAAAGGAGTTTTACAAAAGGAGAC CAACAATCCCGGTGTCCGCCTATTTCAGTACAAGCCTGGTGATTACACATTGCTGGACATGGTGCAGTAT TACTTGAACTTGACAGAAGCCAATCTAAAAGGAGAATCCAACTGGACATTGGAGTATGTCTTGACTCAGG CCTACAGTGTTGCAGATCTGCAGCCAAAGAGTTTATATGCCTTAGTTCAGCAATTTGCCACCAAAGACAG CAAGCAGTTCCTGAAATACTACCATTATTATTTTGTGAGTTATGACAGCAGTGCAACTTGTGACCAGCAT TGTAAGACCTTACAGGTCTGTGCAATTATGAATCTTGATAGCATGTCCTATGATGATTGCCTTAAACAGC ATTTA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG214746 representing NM_020561
Red=Cloning site Green=Tags(s) MALLGNFLCCLLVAWLCGPGLGVPLAPADRAPAVGQFWHVTDLHLDPTYHITDDRTKVCASSKGANASNP GPFGDVLCDSPYQLILSAFDFIKNSGQEASFMIWTGDSPPHVPVPELSTGTVIKVITNMTMTVQNLFPNL QVFPALGNHDYWPQDQLPIVTSKVYSAVADLWKPWLGEEAISTLKKGGFYSQKVASNPGLRIISLNTNLY YGPNIMTLNKTDPANQFEWLENTLNSSLWNKEKVYIIAHVPVGYLPYATDTPAIRQYYNEKLLDIFRRYS SVIAGQFYGHTHRDSLMVLSDKNGNPLNSVFVAPAVTPVKGVLQKETNNPGVRLFQYKPGDYTLLDMVQY YLNLTEANLKGESNWTLEYVLTQAYSVADLQPKSLYALVQQFATKDSKQFLKYYHYYFVSYDSSATCDQH CKTLQVCAIMNLDSMSYDDCLKQHL TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_020561 |
| ORF Size | 1335 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_020561.2, NP_065586.3 |
| RefSeq Size | 1791 bp |
| RefSeq ORF | 1338 bp |
| Locus ID | 57319 |
| UniProt ID | P70158 |
| Gene Summary | Has in vitro nucleotide phosphodiesterase activity with nucleoside triphosphates, such as ATP (PubMed:26792860). Has in vitro activity with p-nitrophenyl-TMP. Has lower activity with nucleoside diphosphates, and no activity with nucleoside monophosphates. Has in vitro activity with CDP-choline, giving rise to CMP and phosphocholine. Has in vitro activity with CDP-ethanolamine. Does not have sphingomyelin phosphodiesterase activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC215990 | Smpdl3a (untagged) - Mouse sphingomyelin phosphodiesterase, acid-like 3A (Smpdl3a), (10ug) |
CNY 5,800.00 |
|
| MR214746 | Smpdl3a (Myc-DDK-tagged) - Mouse sphingomyelin phosphodiesterase, acid-like 3A (Smpdl3a) |
CNY 3,656.00 |
|
| MR214746L3 | Lenti ORF clone of Smpdl3a (Myc-DDK-tagged) - Mouse sphingomyelin phosphodiesterase, acid-like 3A (Smpdl3a) |
CNY 5,230.00 |
|
| MR214746L4 | Lenti ORF clone of Smpdl3a (mGFP-tagged) - Mouse sphingomyelin phosphodiesterase, acid-like 3A (Smpdl3a) |
CNY 5,230.00 |
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