Kif23 (NM_024245) Mouse Tagged ORF Clone
CAT#: MG214019
- TrueORF®
Kif23 (tGFP-tagged) - Mouse kinesin family member 23 (Kif23), (10ug)
ORF Plasmid: DDK
"NM_024245" in other vectors (4)
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CNY 9,220.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 3110001D19Rik; C87313; CHO1; Knsl5; MKLP-1; MKLP1 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG214019 representing NM_024245
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGTCAGCGAAGGCTAAGACGGTCAGAAAACCTGTAATAAAAAAAGGATCTCAGACAAACCTTAAAG ATCCAGTGGGGGTGTACTGTAGGGTTCGCCCCCTGAGCTTTCCTGACCAAGAATGCTGTGTGGAAGTGAT CAATAGTACAACCCTGCAGCTCCACACTCCAGAGGGCTATCGACTCAACAGAAATGGGGACTATAAGGAG ACTCAGTACTCATTCAAGCGGGTATTTGGCACTCACACCACCCAGAAGGAACTTTTTGATGTTGTAGCTA ATCCCTTGGTAGATGACCTCATTCATGGCAAGAATGGTCTTTTATTTACATATGGCGTGACGGGAAGTGG AAAAACCTACACAATGACAGGGTCTCCAGGGTCCGGAGGCCTGCTTCCTCGTTGTTTGAATATGATCTTT AACAGCATAGGGTCATTTCAAGCAAAACGTTATGTGTTTAAGTCTAATGATAGGAACAGTATGGAAATAC AGTGTGAAGTCGATGCCTTGTTAGAACGGCAGAAAAGAGAAGCCTTGCCCATTCCAAAGACCCCCTCTAG CAAGCGACAAGCAGATCCAGAGTTTGCAGATATGATAAATGTACAAGAATTCTGCAAAGCAGAAGAAGTT GATGAAGACAGTGTCTATGGAGTATTTGTCTCTTACATTGAAATCTATAATAATTACATATATGATCTAT TGGAAGAAGTGCAGTTTGATCCCATAAAGCCCAAACTCCCACAATCTAAAACGCTCCGAGAAGATAAGAA CCACAATATGTATGTGGCAGGATGTACAGAAGTAGAAGTGAAATCTACGGAGGAGGCTTTTGAGGTTTTC TGGAGAGGGCAGAAAAAGAGACGCATTGCTAACACCCATTTGAATAGAGAGTCCAGTCGTTCACATAGCG TGTTCAGCATTAAACTTGTCCAGGCTCCCCTGGATGCTGATGGAGACAATGTCTTACAGGAAAAAGAGCA AATTACTATAAGCCAGCTGTCTCTGGTAGATCTTGCTGGGAGCGAAAGAACCAACCGTACTAAAGCAGAA GGGAACAGACTACGTGAGGCCGGAAACATTAATCAGTCATTGATGACACTAAGAACATGCATGGAGGTCC TGAGAGAGAACCAGACGTATGGCACTAACAAGATGGTTCCATATCGAGATTCAAAGCTAACCCATCTATT CAAGAACTACTTCGATGGGGAGGGGAAAGTTCGGATGATCGTGTGTGTGAATCCAAAGGCTGAAGACTAT GAAGAAAGCTTGCAAGTCATGAGATTTGCTGAAGTAACCCAAGAAGTGGAAGTGGCAAGACCAGTGGACA AGGTGATATGTGGCCTGACACCCGGGAGGCGGTACAGGAACCTGCCTCGGGGAGGCCCCATTGGAGATGA ACCTTTGGTGCCTGAAGTGATTCTACAGAGCTTCCCACCGCTGCCCCCATGCAAGCTTTTGGATATCAAT GATGAGGAGACCCTTCCAAAGCTGGCTGACACTTTGGAGAAACGACATCACCTGCGACAACTAATGACCG AGGACCTTAACAAAAAATGTCTAGCTTTCAAGGCCTTATTAAAAGAATTTGACAATTCTCTATCAAATAA AGAAAACTACGTTCAGGAAAAACTAAATGAAAGAGAAAAAGTGATCTCGGGACAGAAATTGGAAATAGAG CGACTGGAGAAGAAAAACAAAACTTTGGAGTACAAGATTGAGATTCTGGAGAAAACAACTACGATCTATG AGGAAGATAAGCGCAATCTGCAGCAGGAGCTTGAGAGCCAGAATCAGAAGCTTCAGCGGCAGTTTTCTGA CAAGCGCAGATTAGAAGCCAGGTTGCAAGGCATGGTGACAGAAACGTCGATGAAGTGGCAGAAGGAATGT GAGCGTCGGGTGGCAGCCACCCAGCTAGAGATGCAGAATAAACTCTGGGTCAAAGATGAAAAGCTCAAAC AGCTGAAGGCCATTGTGACTGAACCCAAACCTGAGAAGCCAGAGAGACCCTCCCGGGAGCGGGACCGGGA GAAAATCATTCCGAGATCTGTCTCTCCTTCGCCTCTACCTCTTTCTAGTAACAATATTGCTCAGATTTCC AACGGCCAGCAACTCATGAGCCAGCCGCAGCTACACAGACGCTCTAACTCTTGCAGCAGCATTTCTGTAG CTTCCTGTATCTCGGAATGGGAGCAGAAACTATCTCCATTCAGCACACCTGTCAATGTCACCTCTCTTGC AAGGCATAGGCAGCAGGAGCCAGGACAAAGTAAAACGTGTATCGTGTCAGACAGAAGGCGAGGCATGTGC TGGACTGAAGGCAGGGAGATGGTCCCCACATTCAGCAGTGAGATAGGCGTAGAAGAGGACCATTGCCGCA GGAACACCCCAATTCCTGTACGACACAGAAGGTCCCGCTCTGCAGGGAGCAGATGGGTAGATCATAAGCC TGCCTCTAATGTGCAAACTGAGACAGTGATGCAGCCGCATGTCCCTCACGCCATCACAGTGTCTGTTGCA AATGAAAAGGCGCTAGCTAAGTGTGAGAAGTACATGCTGACCCACCAGGAACTAGCCTCCGATGGGGAGA TTCAGACTAAAGTCATTAAGGGTGATGTTTATAAGACGAGAGGTGGCGGACAATCGGTTCAGTTTACTGA TATTGAGACTTTAAAACAAGAATTGCCAACTGGTAGTCGGAAACGAAGATCGTCCACCCTAGCACCTGCC CAACCAGATGGTACAGAGTCTGAATGGACCGATGTAGAAACAAGGTGCTCTGTTGCCGTTGAAATGAGAG CAGGATCTCAGCTGGGACCGGGATATCAGCATCACGCACAACCGAAGCGCAAGAAACCT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG214019 representing NM_024245
Red=Cloning site Green=Tags(s) MKSAKAKTVRKPVIKKGSQTNLKDPVGVYCRVRPLSFPDQECCVEVINSTTLQLHTPEGYRLNRNGDYKE TQYSFKRVFGTHTTQKELFDVVANPLVDDLIHGKNGLLFTYGVTGSGKTYTMTGSPGSGGLLPRCLNMIF NSIGSFQAKRYVFKSNDRNSMEIQCEVDALLERQKREALPIPKTPSSKRQADPEFADMINVQEFCKAEEV DEDSVYGVFVSYIEIYNNYIYDLLEEVQFDPIKPKLPQSKTLREDKNHNMYVAGCTEVEVKSTEEAFEVF WRGQKKRRIANTHLNRESSRSHSVFSIKLVQAPLDADGDNVLQEKEQITISQLSLVDLAGSERTNRTKAE GNRLREAGNINQSLMTLRTCMEVLRENQTYGTNKMVPYRDSKLTHLFKNYFDGEGKVRMIVCVNPKAEDY EESLQVMRFAEVTQEVEVARPVDKVICGLTPGRRYRNLPRGGPIGDEPLVPEVILQSFPPLPPCKLLDIN DEETLPKLADTLEKRHHLRQLMTEDLNKKCLAFKALLKEFDNSLSNKENYVQEKLNEREKVISGQKLEIE RLEKKNKTLEYKIEILEKTTTIYEEDKRNLQQELESQNQKLQRQFSDKRRLEARLQGMVTETSMKWQKEC ERRVAATQLEMQNKLWVKDEKLKQLKAIVTEPKPEKPERPSRERDREKIIPRSVSPSPLPLSSNNIAQIS NGQQLMSQPQLHRRSNSCSSISVASCISEWEQKLSPFSTPVNVTSLARHRQQEPGQSKTCIVSDRRRGMC WTEGREMVPTFSSEIGVEEDHCRRNTPIPVRHRRSRSAGSRWVDHKPASNVQTETVMQPHVPHAITVSVA NEKALAKCEKYMLTHQELASDGEIQTKVIKGDVYKTRGGGQSVQFTDIETLKQELPTGSRKRRSSTLAPA QPDGTESEWTDVETRCSVAVEMRAGSQLGPGYQHHAQPKRKKP TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_024245 |
ORF Size | 2859 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_024245.3 |
RefSeq Size | 3439 bp |
RefSeq ORF | 2862 bp |
Locus ID | 71819 |
UniProt ID | E9Q5G3 |
Gene Summary | Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Essential for cytokinesis in Rho-mediated signaling. Required for the localization of ECT2 to the central spindle. Plus-end-directed motor enzyme that moves antiparallel microtubules in vitro (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC222789 | Kif23 (untagged) - Mouse kinesin family member 23 (Kif23), (10ug) |
CNY 12,160.00 |
|
MR214019 | Kif23 (Myc-DDK-tagged) - Mouse kinesin family member 23 (Kif23) |
CNY 6,976.00 |
|
MR214019L3 | Lenti ORF clone of Kif23 (Myc-DDK-tagged) - Mouse kinesin family member 23 (Kif23) |
CNY 10,260.00 |
|
MR214019L4 | Lenti ORF clone of Kif23 (mGFP-tagged) - Mouse kinesin family member 23 (Kif23) |
CNY 10,260.00 |