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Dlg5 (NM_027726) Mouse Tagged ORF Clone
CAT＃: MG214018
- TrueORF®
Dlg5 (tGFP-tagged) - Mouse discs large homolog 5 (Drosophila) (Dlg5) transcript variant 2, (10ug)
ORF Plasmid: DDK
"NM_027726" in other vectors (2)
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CNY 20,810.00
货期*
9周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 4933429D20Rik; mKIAA0583; T25557 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG214018 representing NM_027726
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGCCGCAGCGCCGGGAGCTGCTCGCCCAGTGTCAGCAGAGCCTGGCCCAGGCCATGACCGAGGTGG AGGCCGTGCTTGGGCTGCTGGAGGCAGCAGGAGCGCTGAGCCCCGGCGAGCGGCGGCAGCTGGACGAGGA GGCGGGAGGCGCCAAGGCGGAGCTGCTGCTCCAGCTCCTCCTGGCCAAGGAGCAGGACCACTTCCAGGAC CTGCGCGCCGCCCTGGAGAAGACTCAGCCTCACCTGCTGCCTATTCTCTACCTGAACGGCGTCGTCGGGC CGCCGCAGTCTACAGAGGGCGCGGGGACTACTGGGAAGGCACCGTCCCCACCACCCCTCCTCACCGAGCA GCAGGCCAATGACACGGTGGAGAACCTCTCCATTCAACTAAGGCTGATGACCCGTGAGAGGAATGAGCTA CGCAAGCGCCTGGCCTTCGCCACCCATGGAGCCACCTTTGACAAAAGGCCCTACCACAGGCTGAATCCTG ATTATGAGAGGCTAAAGATCCAGTGTGTGCGGGCCATGTCGGACCTGCAGAGCTTACAGAACCAGCACAC CAACGCCTTGAAGAGGTGCGAGGAGGTGGCCAAGGAGACAGACTTCTATCACACCCTCCACAGCCGGCTC CTGAGTGACCAGACGCAGCTGAAGGACGATGTGGACATGCTGAGACGGGAGAACGGGAAGCTGCGGAGGG AGCGCAATCTGCTGCAGCAGTCCTGGGAGGACATGAAGCGGCTCCGGGAAGAGGACCAGAAGGAGATAGG TGACCTCCGGGCCCAGCAGCAGCAGGTGCTGAAGCACAATGGCTCATCAGAGATCCTCAACAAGCTGTAC GACACGGCCATGGACAAGCTGGAGGTAGTCAAGAAGGACTACGATGCCCTGCGGAAGAGATACAGCGAGA AGGTTGCCATGCACAACTCGGACCTGAGCCGCCTGGAGCAGCTGGGGGAGGAGAACCAGCGTCTGCAGAA GCAGACGGAGATGCTGACCCAGCAGAGGGACACGGCCATCCAGTTACAGCACCAGTGCGCACTCTCCCTG AGGAGGTTTGAGACGATCCACCATGAGCTGAGCAAGGCCACAGCCCAGAACAAGGACCTGCAGTGGGAGA TGGAGCTGCTGCAATCCGAGCTCACGGAGCTGAGGAGCAAGCAGGTGAAAACCGCCAAGGAGTCAGAGAA ATACAAGGAGGAGCGCGATGCTGTGTACAGCGAGTACAAGCTCATCATGAGCGAACGCGACCAGGTCATC TCCGAGCTGGACAAGCTACAGACAGAGGTAGAGCTGGCCGAGTCCAAGCTCAAAAGCAGCACATCCGAGA AGAAGGCAGCCAGCGAGGAGATGGAGGCCCTGCGGCAGATCAAAGACACAGTGACCATGGATGCTGGGAG AGCCAACAAAGAGGTGGAAATCCTCCGAAAACAGTGTAAGGCCTTGTGCCAGGAGCTGAAGGAAGCCCTC CAGGAAGCAGACGTGGCCAAGTGCCGGCGGGACTGGGCCTTTCAGGAGCGGGACAAGATTGTGGCAGAGC GTGACAGCATCAGGACCCTGTGTGACAACCTGAGGCGGGAGCGAGATCGAGCTGTGAGTGAGCTGGCTGA AGCCCTTCGCAGCCTGGATGACACTCGGAAGCAGAAGAATGACGTCAGCCGGGAGCTCAAGGAGCTCAAG GAGCAGATGGAATGTCAGCTGGAGAAGGAGGCCCGATTCCGGCAGCTGATGGCCCACAGTTCGCACGACT CAGCCATCGACACGGATTCCATGGAGTGGGAGACAGAAGTTGTGGAATTTGAGAGAGAGACGGAGGATAT TGACTTGAAAGCACTTGGGTTTGACATGGCAGAAGGCGTGAATGAGCCTTGTTTCCCAGGGGATTGCGGC ATATTTGTTACTAAAGTGGATAAAGGAAGCATTGCTGATGGTCGCTTAAGGGTCAACGACTGGCTGCTGA GAATCAACGATGTAGACCTCATCAACAAGGACAAGAAACAGGCCATCAAGGCACTCCTCAACGGAGAGGG AGCCATCAACATGGTTGTGAGGCGGAGGAAGTCCCTGGGTGGGAAGGTCGTCACACCTCTGCACATCAAC CTCAGTGGACAGAAAGACAGTGGCATCAGTTTGGAGAATGGAGTGTATGCTGCCGCAGTGGTGCCTGGAA GCCCGGCTGCCAAAGAGGGGTCCCTCGCCGTGGGAGACAGGATTGTTGCGATCAACGGTATTGCACTGGA CAATAAGTCTCTAAATGAATGTGAATCTCTGCTGCGCAGCTGTCAAGACTCCTTGACCCTCTCCCTCCTG AAGGTGTTTCCCCAGAGCTCCTCATGGAGTGGCCAGAACATCTTTGAAAATATCAAGGACTCAGATAGGA TGCTGAGTTGCCGAGCCCACGGCCCAGAGGTCCAGGCGCATAACAAACGGAACTTGTTACAGCACAATAA TTCCACGCAGACAGACATTTTCTACACGGATCGGCTGGAGGACAGGAAGGAGCTGGGACATTCTGGAGGC AGCAGTTCTTTTCTCCACAAGCCCTTCTCTGGATCCTCGTCTCCAGTCTCCCCTCAGGCCTGTCCTAGCA CCTCTGAGCGTAGTCTGAACTCATTCCGCTCTGATACCTCTGCAGAACGTGGCTATGGGCTGGTGGATAT GCGGAGCCAGCGGCCACTGCTGTCTTTTGAGACTGAGGTGGGCCCCTGTGGGGCAGTAGAGGTTCCCCTG GACAAGATAGATCCAGAGGGCTCCAATAGTGGTGGGACCTGGCCCAAGGCTGTGCTTGGCTCCACATCAG GGCCTGAGAAACTCTCTGTGTATAAGAAACCCAAACAAAGGAAGTCCATCTTTGACCCAAATACTTTCAA GCGCCCACAGACACCCCCCAAAATAGACTACCTGCTTCCAGGCCCTGGGCTTACTCATTCCCCCCAGCCC TCCAAGAGGGTAGGATCTCTGACACCCCCAAAGCCCCCCAGGAGAAGTGACTCTATCAAGTTCCAACACA GGCTGGAGACCAGTTCTGAGTCAGAAGCCACTTTGGTGGGCAGCTCACCTTCCACCAGCCCTCCCAGTGC CCCACCCCCCAGCATGGATCCCAGTGAGCCCACGCATGCTTCACCTCCTCGGAAGGCCAGGGTCCGCATT GCCTCCAGCTACCACTCTGAAGGGGACGGAGACACCTCCTACCTGCCCGCCAAGAAGCCTTGTGATGAAG ACCTCACCTCCCAGAAAGTCGATGAGCTGGGGCAGAAACGGCGCCGGCCAAAGTCTGCCCCAAGTTTTCG GCCCAAGATCTCTCCAGTGGTGATCCCTGCTCAGTGCCTGGAGGAACAGGAGTGTGTTCCAGCCATCGGA GAGCTCTCCCCAGAGGGTCAGGAATGGTCCCCGTACTCACCAGGGCATGCTAGCCGGCATGGCAACCCCC TACTCTACCCCAACCGGCCATCTGTGGGCACCGTTCCCCGGAGTATGACCCCCGGCACCACTGTGGGCTC CATTCTGAGGAACCCCATTTATACTGTGCGCAGCCACAGGGTGCTTCCCTGCGGCTCACCACCCGTGCCC CGTGATGCTGGCTCCCAGAGCTTGTCTCCCAGTGTCCAGCATCAGGGACGCCTCAGCCTGGACCTGAGCC ACAGGGCCTGCAGTGACTACTCTGAGATGAGAGCCTCCCAGGGTTCCAACTCTCTGCCCTCCAGTGCCCG CCTTGGCTCTTCCAGTAACCTGCAGTTCAAGGCTGAGCGGATTAAGATCCCGCTAACACCAAGATATCCC CGGTCTGTCATGGGCTCTGACAGAGGCTCACTGTCACATTCTGAATGTAGCACTCCTCCCCGGTCCCCGC TGAATATCGACACCCTGTCCTCCTGTAGCCAGCCTCAGACCACAGCCTCCACTTTACCCAGGATTGCGGT CAACCCCTCTTCCCATGGGGAGCGGAGGAAGGACAGGCCTTTTGTGGAAGAGCCACGCCACGTGAAGGTG CAGAAGGGTTCAGAGCCTCTGGGTATCTCCATCGTGAGTGGAGAAAAGGGTGGTGTCTATGTCTCCAAGG TGACCCTGGGGAGCATCGCTCATCAAGCTGGACTTGAGTATGGGGACCAGTTACTTGAGTTCAATGGCAT AAACCTGAGAAGTGCCACCGAGCAGCAGGCCCGGCTCATCATCGGACAGCAGTGTGACACCATCACCATC CTGGCCCAGTACAACCCACACATCCACCAGCTCAACAGCCACTCCCGCTCCAGCTCTCATCTGGACCCAG CTGCTACCCCTCACTCTACTCTCCAGGGCAGCAGTGCTGGGACCCCAGAACATCCCTCTGTCATCGACCC ACTGATGGAACAGGATGAAGGTCCTGGCACCCCCCCAGCCAAGCAAAGCGCCTCCAGCACCAGGAGTGTG GGAGATACCACCAAGAAGACCCCTGATCCCCGCATTGTTTTCATCAAGAAATCCCAGCTGGATCTGGGGG TGCACTTGTGTGGTGGGAACCTGCACGGAGTGTTTGTGGCCGAAGTGGAAGATGACAGTCCTGCCAAGGG TCCTGATGGCCTCGTCCCAGGAGACCTCATCCTTGAGTATGGCAGCCTGGATATGCGCAGCAGGACAGTG GAGGACGTCTATGTGGAGATGCTGAAGCCTAAGGATAGCCTTCGCCTGAAGGTGCAGTACCGCCATGAGG AGTTCACCAGGGTCAAGGGCCTGCCCGGAGACAGCTTCTACATCAGGGCCCTATATGACCGTCTGGCAGA GGTGGAGCCTGAACTGAGTTTTAAGAAAGATGACATCCTGTACGTGGATGATACGTTGCCCCAGGGCGTG TTTGGGTCCTGGATGGCCTGGCAGCTGGATGAGAATGCCCAGAAGATCCAGCGTGGGCAGATTCCCAGCA AATACGTGATGGACCAAGAGTTCTCCCGGAGGCTCAGCATGTCTGAGGTCAAAGATGACAACACAGCCAA GACGCTGTCAGCAGCTGCGCGCCGTTCCTTCTTCCGAAGGAAACATAAACATAAACGCAGTGGGTCCAAA GACGGCAAGGACCTGCTCGCCCTGGACACCTTTTCCAACGACTCCATTCCTCTCTTTGAAGACTCAGTGA GCCTGGCCTATCAGCGGGTCCAGAAAGTGGACTGTACCTCTCTTCGGCCTGTTCTCCTCCTGGGACCTTT ACTGGATGTGGTGAAAGAGATGCTGGTGAATGAGGCACCTGGCAAGTTCTGCAGATGCCCACTTGAGGTG ATGAAGGCTTCCCAGCAAGCCATTGAGCGGGGTGTCAAAGACTGCCTGTTTGTGGACTACAAGCGGAGGA GTGGCCATTTTGATGTTACTACTGTGGCTTCAATAAAGGAGATCACAGAAAAGAACCGGCACTGTCTTCT GGACATCGCCCCGCATGCCATCGAGAGGCTGCACCACATGCACATCTACCCCATTGTCATCTTCATCCGC TACAAGAGCGCCAAGCACATCAAGGAACAGAGAGACCCTGTCTACCTGAGGGACAAGGTGACACAGAGGC ATTCCAAAGAGCAATTCGAAACAGCACAGAAGATCGATCAGGAGTACAGCAGGTACTTCACAGGGGTTGT CCAGGGTGGAGCCCTGTCCAGCATTTGCACTCAGATCCTGGCCATGGTCAGTCAAGAACAAAGTAAAGTC CTGTGGATCCCCGCTTGCCCACCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG214018 representing NM_027726
Red=Cloning site Green=Tags(s) MEPQRRELLAQCQQSLAQAMTEVEAVLGLLEAAGALSPGERRQLDEEAGGAKAELLLQLLLAKEQDHFQD LRAALEKTQPHLLPILYLNGVVGPPQSTEGAGTTGKAPSPPPLLTEQQANDTVENLSIQLRLMTRERNEL RKRLAFATHGATFDKRPYHRLNPDYERLKIQCVRAMSDLQSLQNQHTNALKRCEEVAKETDFYHTLHSRL LSDQTQLKDDVDMLRRENGKLRRERNLLQQSWEDMKRLREEDQKEIGDLRAQQQQVLKHNGSSEILNKLY DTAMDKLEVVKKDYDALRKRYSEKVAMHNSDLSRLEQLGEENQRLQKQTEMLTQQRDTAIQLQHQCALSL RRFETIHHELSKATAQNKDLQWEMELLQSELTELRSKQVKTAKESEKYKEERDAVYSEYKLIMSERDQVI SELDKLQTEVELAESKLKSSTSEKKAASEEMEALRQIKDTVTMDAGRANKEVEILRKQCKALCQELKEAL QEADVAKCRRDWAFQERDKIVAERDSIRTLCDNLRRERDRAVSELAEALRSLDDTRKQKNDVSRELKELK EQMECQLEKEARFRQLMAHSSHDSAIDTDSMEWETEVVEFERETEDIDLKALGFDMAEGVNEPCFPGDCG IFVTKVDKGSIADGRLRVNDWLLRINDVDLINKDKKQAIKALLNGEGAINMVVRRRKSLGGKVVTPLHIN LSGQKDSGISLENGVYAAAVVPGSPAAKEGSLAVGDRIVAINGIALDNKSLNECESLLRSCQDSLTLSLL KVFPQSSSWSGQNIFENIKDSDRMLSCRAHGPEVQAHNKRNLLQHNNSTQTDIFYTDRLEDRKELGHSGG SSSFLHKPFSGSSSPVSPQACPSTSERSLNSFRSDTSAERGYGLVDMRSQRPLLSFETEVGPCGAVEVPL DKIDPEGSNSGGTWPKAVLGSTSGPEKLSVYKKPKQRKSIFDPNTFKRPQTPPKIDYLLPGPGLTHSPQP SKRVGSLTPPKPPRRSDSIKFQHRLETSSESEATLVGSSPSTSPPSAPPPSMDPSEPTHASPPRKARVRI ASSYHSEGDGDTSYLPAKKPCDEDLTSQKVDELGQKRRRPKSAPSFRPKISPVVIPAQCLEEQECVPAIG ELSPEGQEWSPYSPGHASRHGNPLLYPNRPSVGTVPRSMTPGTTVGSILRNPIYTVRSHRVLPCGSPPVP RDAGSQSLSPSVQHQGRLSLDLSHRACSDYSEMRASQGSNSLPSSARLGSSSNLQFKAERIKIPLTPRYP RSVMGSDRGSLSHSECSTPPRSPLNIDTLSSCSQPQTTASTLPRIAVNPSSHGERRKDRPFVEEPRHVKV QKGSEPLGISIVSGEKGGVYVSKVTLGSIAHQAGLEYGDQLLEFNGINLRSATEQQARLIIGQQCDTITI LAQYNPHIHQLNSHSRSSSHLDPAATPHSTLQGSSAGTPEHPSVIDPLMEQDEGPGTPPAKQSASSTRSV GDTTKKTPDPRIVFIKKSQLDLGVHLCGGNLHGVFVAEVEDDSPAKGPDGLVPGDLILEYGSLDMRSRTV EDVYVEMLKPKDSLRLKVQYRHEEFTRVKGLPGDSFYIRALYDRLAEVEPELSFKKDDILYVDDTLPQGV FGSWMAWQLDENAQKIQRGQIPSKYVMDQEFSRRLSMSEVKDDNTAKTLSAAARRSFFRRKHKHKRSGSK DGKDLLALDTFSNDSIPLFEDSVSLAYQRVQKVDCTSLRPVLLLGPLLDVVKEMLVNEAPGKFCRCPLEV MKASQQAIERGVKDCLFVDYKRRSGHFDVTTVASIKEITEKNRHCLLDIAPHAIERLHHMHIYPIVIFIR YKSAKHIKEQRDPVYLRDKVTQRHSKEQFETAQKIDQEYSRYFTGVVQGGALSSICTQILAMVSQEQSKV LWIPACPP TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_027726 |
| ORF Size | 5694 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_027726.1, NP_082002.1 |
| RefSeq Size | 7809 bp |
| RefSeq ORF | 5697 bp |
| Locus ID | 71228 |
| UniProt ID | E9Q9R9 |
| Gene Summary | Acts as a regulator of the Hippo signaling pathway. Negatively regulates the Hippo signaling pathway by mediating the interaction of MARK3 with STK3/4, bringing them together to promote MARK3-dependent hyperphosphorylation and inactivation of STK3 kinase activity toward LATS1 (PubMed:28087714). Positively regulates the Hippo signaling by mediating the interaction of SCRIB with STK4/MST1 and LATS1 which is important for the activation of the Hippo signaling pathway. Involved in regulating cell proliferation, maintenance of epithelial polarity, epithelial-mesenchymal transition (EMT), cell migration and invasion (By similarity). Plays an important role in dendritic spine formation and synaptogenesis in cortical neurons; regulates synaptogenesis by enhancing the cell surface localization of N-cadherin (PubMed:25232112). Acts as a positive regulator of hedgehog (Hh) signaling pathway. Plays a critical role in the early point of the SMO activity cycle by interacting with SMO at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation (PubMed:25644602).[UniProtKB/Swiss-Prot Function] |
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