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Pole (NM_011132) Mouse Tagged ORF Clone
CAT＃: MG212119
- TrueORF®
Pole (tGFP-tagged) - Mouse polymerase (DNA directed), epsilon (Pole)
ORF Plasmid: DDK
"NM_011132" in other vectors (2)
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CNY 27,550.00
货期*
3周
规格
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经常一起买 (4)
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG212119 representing NM_011132
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCTGAGGAACAGTGGACGGAGGCACCCCGAGCCGGGCGCGGATGGCGAAGGCAGCCGGGATGATG GTCCCTCTTCCTCAGTCTCAGCACTCAAGCGTCTGGAACGGAGCCAGTGGACAGACAAGATGGACTTACG GTTTGGTTTCGAAAGGCTGAAAGAGCCTGGAGAAAGGACTGGCTGGCTGATCAACATGCACCCTACTGAG ATCTTAGATGAAGACAAACGCTTAGTCAGCGCGGTGGATTACTACTTCATTCAAGATGATGGAAGCAGAT TTAAGGTGGCCTTGCCCTATATGCCGTATTTCTACATTGCAGCGAGAAAGGGTTGTGATCGAGAAGTTTC ATCTTTTCTATCCAAGAAGTTTCAGGGAAAAATTGCAAAGTTAGAGAATGTGCCCAAAGAAGATCTGGAC TTGCCAAATCACTTGGTGGGCTTGAAGCGGAGTTACATCAAGCTGTCCTTCCACACTGTGGAGGACCTTG TCAAAGTGAGAAAGGAGATCTCTCCTGCTGTGAAGAAGAACCGAGAGCAGGACCATGCTAGTGATGAGTA TACAACAATGCTCTCCAGTATTCTGCAAGGTGGCAGTGTAATTACTGATGAGGACGAAACCTCTAAGAAG ATAGCTGACCAATTGGACAACATAGTGGACATGCGAGAGTATGATGTTCCCTACCACATTCGCCTCTCCA TTGACCTCAGAATCCATGTGGCCCACTGGTACAATGTTAGATTTCGAGGAAATGCTTTTCCTGTGGAAAT CACCCGACGAGATGATCTTGTTGAACGACCTGACCCTGTGGTTTTGGCATTTGACATCGAGACGACCAAA CTGCCTCTCAAATTCCCTGATGCTGAGACCGATCAGATCATGATGATCTCCTATATGATTGATGGCCAGG GCTACCTCATCACTAACAGGGAGATTGTTTCAGAAGATATTGAAGATTTTGAGTTCACCCCTAAGCCAGA ATATGAAGGGCCCTTTTGTGTCTTCAATGAACCCGACGAGGTCCATCTGATCCAGAGATGGTTTGAGCAT ATCCAGGAAACCAAACCTACCATTATGGTCACCTACAATGGGGATTTTTTTGACTGGCCATTTGTGGAGG CTAGGGCAGCAATTCATGGCCTCAGCATGTACCAGGAGATAGGCTTCCAGAAGGATAGCCAGGGGGAATA TAAGGCACCACAGTGCATCCACATGGACTGCCTCAGGTGGGTGAAGAGGGACAGTTACCTTCCTGTGGGC AGTCATAATCTCAAGGCAGCTGCCAAGGCCAAACTTGGCTATGACCCTGTAGAGCTGGACCCTGAGGACA TGTGTCGTATGGCCACTGAACAGCCCCAGACTCTGGCCACTTACTCAGTGTCAGATGCTGTGGCTACTTA CTACCTGTACATGAAATACGTCCACCCCTTCATATTCGCCCTGTGCACCATTATTCCCATGGAACCTGAT GAGGTGCTGCGGAAGGGCTCCGGGACACTGTGTGAAGCCTTGCTGATGGTGCAAGCTTTCCATGCCAACA TTATCTTCCCCAATAAGCAAGAGCAGGAGTTCAACAAGCTGACAGATGATGGCCACATGCTAGATGCTGA GACCTACGTCGGGGGCCACGTGGAGGCACTAGAGTCTGGTGTCTTCAGAAGTGATATCCCCTGCCGGTTT AGGATGAATCCTGCAGCCTTTGATTTCCTGCTGCAACGAGTCGAGAAGACTATGCGCCACGCCATTGAAG AAGAAGAGAAGGTGCCTGTGGAACAAGCCACCAACTTTCAAGAGGTGTGTGAGCAGATTAAGACCAAGCT CACCTCCCTAAAAGATGTTCCTAACAGAATTGAATGTCCTCTAATCTACCATCTAGATGTGGGGGCCATG TATCCTAACATAATTCTTACCAACCGCCTACAGCCTTCTGCCATAGTGGATGAGGCCACCTGTGCTGCCT GTGACTTCAATAAGCCTGGAGCAAGTTGTCAGAGGAAGATGGCCTGGCAGTGGAGGGGAGAATTCATGCC AGCCAGTCGCAGTGAATACCATCGGATTCAGCATCAGCTGGAGTCGGAGAAGTTTCCCCCTTTGTTTCCA GAGGGGCCAGCACGGGCCTTTCACGAGCTGTCCCGTGAAGAACAGGCTAAATACGAGAAGAGGAGGCTGG CAGATTATTGCCGGAAAGCCTATAAGAAGATCCATGTGACCAAGGTAGAAGAACGTCTAACTACCATCTG CCAGCGGGAAAACTCATTTTATGTGGACACAGTGCGGGCCTTCAGAGACAGGCGCTATGAGTTCAAAGGA CTGCACAAGGTGTGGAAGAAGAAGCTCTCGGCAGCTGTAGAGGTGGGCGATGCATCAGAGGTGAAGCGCT GCAAGAACATGGAGATCCTTTACGATTCACTGCAGCTGGCTCACAAGTGCATCCTGAACTCCTTCTACGG CTATGTCATGCGCAAAGGAGCTCGCTGGTATTCCATGGAGATGGCTGGTATCGTCTGCTTTACAGGAGCC AACATCATCACCCAAGCAAGAGAACTGATTGAGCAGATCGGGAGGCCTTTAGAATTGGACACGGACGGAA TATGGTGCGTCCTACCCAATAGCTTTCCTGAAAATTTTGTCATCAAGACAACCAATGCGAAGAAACCCAA ACTGACCATCTCCTATCCTGGTGCCATGTTGAACATCATGGTCAAGGAAGGCTTTACCAACCACCAGTAC CAGGAACTAACAGAGCCTTCGTCTCTCACCTATGTCACCCACTCTGAGAATAGTATCTTTTTTGAAGTCG ATGGACCATACCTTGCTATGATCCTTCCAGCCTCCAAGGAAGAAGGCAAGAAGCTGAAGAAAAGATATGC TGTGTTCAATGAAGATGGTTCCTTGGCTGAACTGAAAGGTTTTGAGGTGAAACGCCGAGGGGAGTTGCAG CTGATTAAAATATTCCAGTCCTCAGTTTTTGAGGCCTTCCTCAAGGGCAGCACACTGGAGGAAGTGTATG GCTCGGTGGCCAAAGTGGCTGACTACTGGCTAGATGTGCTCTATAGCAAGGCTGCTAATATGCCCGATTC TGAATTGTTTGAGCTGATTTCTGAGAACCGCTCCATGTCTCGGAAGCTGGAAGATTACGGGGAGCAGAAG TCTACATCCATCAGCACAGCAAAGCGCCTGGCTGAGTTCCTGGGAGACCAGATGGTCAAAGATGCTGGAC TGAGCTGCCGCTATATCATCTCCCGAAAGCCAGAGGGGTCTCCTGTCACTGAGAGGGCCATTCCACTTGC CATTTTCCAAGCAGAGCCTACAGTGAGGAAACATTTTCTCCGGAAATGGCTAAAGAGTTCATCACTTCAA GACTTTGATATTCGGACAATTCTGGACTGGGACTACTACATAGAGAGGCTGGGGAGTGCCATCCAGAAAA TCATCACCATCCCCGCAGCTCTGCAGCAGGTGAAGAACCCAGTTCCACGTGTCAAACATCCAGACTGGCT ACACAAAAAACTACTAGAGAAGAATGATATCTACAAACAGAAGAAGATCAGTGAGCTCTTTGTGCTTGAA GGAAAGAGACAGATTGTGATGGCCCAGGCTTCAGAAAACAGTCTGAGTCTCTGCACTCCAGACATGGAGG ACATTGGACTCACAAAGCCACACCACTCTACAGTCCCAGTTGCTACTAAGAGGAAGCGAGTCTGGGAGAC CCAAAAGGAGTCTCAGGATATTGCACTAACTGTGCCCTGGCAAGAGGTCTTAGGGCAGCCTCCCTCCCTT GGAACCACACAGGAAGAGTGGTTGGTCTGGCTCCAGTTCCACAAGAAAAAGTGGCAGCTGCAGGCCCAAC AGCGCCTAGCCCTCAGGAAGAAGCAACGCTTAGAGTCAGCAGAAGATATGCCAAGGCTTGGGCCTATCCG AGAGGGGCCTTCCACAGGACTGGGGAGCTTTTTGCGAAGGACTGCCCGCAGCATCATGGACCTTCCATGG CAGATAATACAGATCAGTGAGACCAGACAGGCTGGTCTGTTCCGGCTGTGGGCTATCATTGGCAATGACT TGCACTGCATCAAGCTGAGTATCCCTCGAGTATTCTATGTAAACCAGCGGGTTGCCAAAGCAGAGGATGG ACCTGCATATCGGAAGGTGAATCGGGCTCTTCCTCGTTCCAACATTGTCTACAATCTCTATGAGTATTCA GTACCAGAGGACATGTACCAAGAACACATCAACGAGATCAACACTGAGTTGTCAGTACCAGACATTGAGG GCGTGTATGAGACACAGGTCCCATTGTTATTCCGGGCCCTCGTGCAGCTGGGCTGTGTGTGTGTGGTCAA CAAGCAGCTGACAAGGCACCTTTCGGGCTGGGAAGCTGAAACTTTTGCCCTCGAGCACCTTGAAATGCGT TCTCTGGCCCAGTTCAGCTACTTGGAACCAGGGAGTATCCGCCATATCTACCTGTACCATCACACTCAGG GCCACAAGGCACTCTTTGGGGTCTTTATCCCCTCACAGCGAAGAGCATCTGTGTTTGTGTTGGATACTGT ACGAAGCAACCAAATGCCAGGGCTCAGTGCCCTGTACTCATCAGAACACAGCCTGCTGCTGGACAAGGTG GACCCCAAGCTCCTGCCTCCCCCAAAACACACCTTTGAAGTTCGTGCTGAAACCAACCTGAAGACTATCT GCAGAGCCATCCAGCGCTTCCTGCTTGCCTACAAGGAAGAGCGCCGAGGGCCCACACTCATCGCTGTCCA GTCTAGCTGGGAGCTGTGTAGGCTGACCAGTGAGATTCCAGTCTTAGAAGAGTTCCCACTAGTGCCTATC CGAGTGGCTGACAAGATCAGCTATGCAGTCCTGGACTGGCAGCGCCATGGAGCTCGCCGAATGATCCGGC ACTACCTCAATTTAGACTTGTGCCTGTCGCAGGCCTTTGAGATGAGCAGGTACTTCCACATCCCTGTTGG AAACCTGCCGGAAGACATCTCCACCTTTGGCTCAGACCTCTTTTTTGCACGCCACCTCCAGCACCATAAC CACCTGCTTTGGCTATCCCCTACCTCTCGGCCTGACCTGGGTGGGAAGGAAGCTGATGACAACCGCCTTG TCATGGAGTTTGATGACCGAGCCACTGTGGAGATCAATAGTTCTGGCTGTTACTCTACTGTGTGCGTGGA ACTGGACATTCAAAATCTGGCAGTCAACACCATCCTCCAGTCCCATCATGTCAATGACATGGAGGGGGCT GGCAGCATGGGCATCAGCTTCGATGTGATCCAGCAGGCCTCCCTAGAGGACATGGTAACAGGCAATCAAG CTGCCAGTGCCCTGGCCAACTACGATGAGACAGCCCTCTGCTCTAGTACCTTCAGGATCCTGAAGAGCAT GGTGGTTGGCTGGGTAAAGGAAATCACACAGTACCACAACATCTATGCTGACAACCAGGTAATGCACTTC TACCGCTGGCTCCAGTCACCGTGCTCTCTGCTCCACGACCCAGCCCTTCACCGGACGCTGCACAACATGA TGAAGAAGCTCTTCCTGCAGCTCATTGCTGAGTTCAAGCGCCTGGGGTCATCAGTCGTCTATGCCAACTT CAATCGCATCATTCTCTGTACAAAGAAGCGCCGAATAGAGGATGCCCTTGCCTATGTGGAATATATTACC AACAGCATCCATTCTAAAGAGATCTTCCATTCCCTGACCATCTCTTTCTCTCGATGCTGGGAATTCCTTC TCTGGATGGATCCATCCAACTATGGTGGAATCAAAGGAAAAGTTCCATCTAGTATTCACTGTGGACAGGT AAAAGAGCAAGACTCCCAGGCAAGAGAGGAAACTGATGAAGAGGAGGAGGACAAGGAAAAGGACGAGGAG GAAGAGGGCATGGGAGAGTCCGAGGTTGAGGACTTACTGGAGAACAACTGGAACATTCTACAGTTCTTGC CCCAGGCAGCCTCTTGCCAGAGCTACTTCCTCATGATTGTTTCAGCATACATCGTAGCTGTGTACCAAAG CATGAAGGAGGAGTTGAGACACAGTGCCCCGGGCAGTACCCCTGTGAAGAGGAAGGGGGCCAGCCAGTTC TCCCAGGAGTCTGAAGGGGCAACTGGATCTCTTCCTGGAATGATCACTTTCTCTCAAGATTATGTGGCAA ATGAGCTCACTCAGAGCTTCTTCACCATTACTCAGAAAATTCAGAAGAAAGTCACAGGTTCTCGGAACAC CACTGAGCCCTCAGAGATGTTCCCCGTCCTCCCTGGTTCACACTTGCTGCTCAATAATCCTGCTCTGGAG TTCATCAAATATGTGTGCAAGGTACTATCTCTGGATACAAACATCACAAATCAGGTGAATAAGCTGAACA GAGACCTTCTTCGCCTGGTAGACGTTGGTGAATTCTCTGAGGAGGCCCAGTTCAGAGACCCCTGCCACTC CTACGTGCTCCCTGAGGTAATCTGCCACAGCTGTAATTTCTGCCGAGACCTGGACCTGTGCAAAGATTCC TCTTTCTCTCAGGATGGAGCCATCCTGCCTCAGTGGCTCTGCTCCAATTGTCAAGCCCCCTATGACTCCT CTGCCATTGAGTCAGCCTTGGTGGAAGCCCTGCAGAGGAAACTGATGGCCTTCACACTTCAGGACCTGGT ATGCCTCAAGTGCCGTGGTATGAAAGAGACCCATATGCCTGTGTACTGCAGCTGCGCAGGGGACTTTACT CTCACCATCCGCACTGAGGTCTTCATGGAACAGATTAGAATCTTCCAGAACATTGCCAAGTACTACAGCA TGTCATATCTCCAGGAGACCATAGAATGGCTGTTACAGACAAGCCCTGTATCAAACTGT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG212119 representing NM_011132
Red=Cloning site Green=Tags(s) MVLRNSGRRHPEPGADGEGSRDDGPSSSVSALKRLERSQWTDKMDLRFGFERLKEPGERTGWLINMHPTE ILDEDKRLVSAVDYYFIQDDGSRFKVALPYMPYFYIAARKGCDREVSSFLSKKFQGKIAKLENVPKEDLD LPNHLVGLKRSYIKLSFHTVEDLVKVRKEISPAVKKNREQDHASDEYTTMLSSILQGGSVITDEDETSKK IADQLDNIVDMREYDVPYHIRLSIDLRIHVAHWYNVRFRGNAFPVEITRRDDLVERPDPVVLAFDIETTK LPLKFPDAETDQIMMISYMIDGQGYLITNREIVSEDIEDFEFTPKPEYEGPFCVFNEPDEVHLIQRWFEH IQETKPTIMVTYNGDFFDWPFVEARAAIHGLSMYQEIGFQKDSQGEYKAPQCIHMDCLRWVKRDSYLPVG SHNLKAAAKAKLGYDPVELDPEDMCRMATEQPQTLATYSVSDAVATYYLYMKYVHPFIFALCTIIPMEPD EVLRKGSGTLCEALLMVQAFHANIIFPNKQEQEFNKLTDDGHMLDAETYVGGHVEALESGVFRSDIPCRF RMNPAAFDFLLQRVEKTMRHAIEEEEKVPVEQATNFQEVCEQIKTKLTSLKDVPNRIECPLIYHLDVGAM YPNIILTNRLQPSAIVDEATCAACDFNKPGASCQRKMAWQWRGEFMPASRSEYHRIQHQLESEKFPPLFP EGPARAFHELSREEQAKYEKRRLADYCRKAYKKIHVTKVEERLTTICQRENSFYVDTVRAFRDRRYEFKG LHKVWKKKLSAAVEVGDASEVKRCKNMEILYDSLQLAHKCILNSFYGYVMRKGARWYSMEMAGIVCFTGA NIITQARELIEQIGRPLELDTDGIWCVLPNSFPENFVIKTTNAKKPKLTISYPGAMLNIMVKEGFTNHQY QELTEPSSLTYVTHSENSIFFEVDGPYLAMILPASKEEGKKLKKRYAVFNEDGSLAELKGFEVKRRGELQ LIKIFQSSVFEAFLKGSTLEEVYGSVAKVADYWLDVLYSKAANMPDSELFELISENRSMSRKLEDYGEQK STSISTAKRLAEFLGDQMVKDAGLSCRYIISRKPEGSPVTERAIPLAIFQAEPTVRKHFLRKWLKSSSLQ DFDIRTILDWDYYIERLGSAIQKIITIPAALQQVKNPVPRVKHPDWLHKKLLEKNDIYKQKKISELFVLE GKRQIVMAQASENSLSLCTPDMEDIGLTKPHHSTVPVATKRKRVWETQKESQDIALTVPWQEVLGQPPSL GTTQEEWLVWLQFHKKKWQLQAQQRLALRKKQRLESAEDMPRLGPIREGPSTGLGSFLRRTARSIMDLPW QIIQISETRQAGLFRLWAIIGNDLHCIKLSIPRVFYVNQRVAKAEDGPAYRKVNRALPRSNIVYNLYEYS VPEDMYQEHINEINTELSVPDIEGVYETQVPLLFRALVQLGCVCVVNKQLTRHLSGWEAETFALEHLEMR SLAQFSYLEPGSIRHIYLYHHTQGHKALFGVFIPSQRRASVFVLDTVRSNQMPGLSALYSSEHSLLLDKV DPKLLPPPKHTFEVRAETNLKTICRAIQRFLLAYKEERRGPTLIAVQSSWELCRLTSEIPVLEEFPLVPI RVADKISYAVLDWQRHGARRMIRHYLNLDLCLSQAFEMSRYFHIPVGNLPEDISTFGSDLFFARHLQHHN HLLWLSPTSRPDLGGKEADDNRLVMEFDDRATVEINSSGCYSTVCVELDIQNLAVNTILQSHHVNDMEGA GSMGISFDVIQQASLEDMVTGNQAASALANYDETALCSSTFRILKSMVVGWVKEITQYHNIYADNQVMHF YRWLQSPCSLLHDPALHRTLHNMMKKLFLQLIAEFKRLGSSVVYANFNRIILCTKKRRIEDALAYVEYIT NSIHSKEIFHSLTISFSRCWEFLLWMDPSNYGGIKGKVPSSIHCGQVKEQDSQAREETDEEEEDKEKDEE EEGMGESEVEDLLENNWNILQFLPQAASCQSYFLMIVSAYIVAVYQSMKEELRHSAPGSTPVKRKGASQF SQESEGATGSLPGMITFSQDYVANELTQSFFTITQKIQKKVTGSRNTTEPSEMFPVLPGSHLLLNNPALE FIKYVCKVLSLDTNITNQVNKLNRDLLRLVDVGEFSEEAQFRDPCHSYVLPEVICHSCNFCRDLDLCKDS SFSQDGAILPQWLCSNCQAPYDSSAIESALVEALQRKLMAFTLQDLVCLKCRGMKETHMPVYCSCAGDFT LTIRTEVFMEQIRIFQNIAKYYSMSYLQETIEWLLQTSPVSNC TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011132 |
| ORF Size | 6849 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011132.2, NP_035262.2 |
| RefSeq Size | 7167 bp |
| RefSeq ORF | 6852 bp |
| Locus ID | 18973 |
| UniProt ID | Q9WVF7 |
| Gene Summary | Participates in DNA repair and in chromosomal DNA replication.[UniProtKB/Swiss-Prot Function] |
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