Pole (NM_011132) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC225257 representing NM_011132 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCTGAGGAACAGTGGACGGAGGCACCCCGAGCCGGGCGCGGATGGCGAAGGCAGCCGGGATGATG GTCCCTCTTCCTCAGTCTCAGCACTCAAGCGTCTGGAACGGAGCCAGTGGACAGACAAGATGGACTTACG GTTTGGTTTCGAAAGGCTGAAAGAGCCTGGAGAAAGGACTGGCTGGCTGATCAACATGCACCCTACTGAG ATCTTAGATGAAGACAAACGCTTAGTCAGCGCGGTGGATTACTACTTCATTCAAGATGATGGAAGCAGAT TTAAGGTGGCCTTGCCCTATATGCCGTATTTCTACATTGCAGCGAGAAAGGGTTGTGATCGAGAAGTTTC ATCTTTTCTATCCAAGAAGTTTCAGGGAAAAATTGCAAAGTTAGAGAATGTGCCCAAAGAAGATCTGGAC TTGCCAAATCACTTGGTGGGCTTGAAGCGGAGTTACATCAAGCTGTCCTTCCACACTGTGGAGGACCTTG TCAAAGTGAGAAAGGAGATCTCTCCTGCTGTGAAGAAGAACCGAGAGCAGGACCATGCTAGTGATGAGTA TACAACAATGCTCTCCAGTATTCTGCAAGGTGGCAGTGTAATTACTGATGAGGACGAAACCTCTAAGAAG ATAGCTGACCAATTGGACAACATAGTGGACATGCGAGAGTATGATGTTCCCTACCACATTCGCCTCTCCA TTGACCTCAGAATCCATGTGGCCCACTGGTACAATGTTAGATTTCGAGGAAATGCTTTTCCTGTGGAAAT CACCCGACGAGATGATCTTGTTGAACGACCTGACCCTGTGGTTTTGGCATTTGACATCGAGACGACCAAA CTGCCTCTCAAATTCCCTGATGCTGAGACCGATCAGATCATGATGATCTCCTATATGATTGATGGCCAGG GCTACCTCATCACTAACAGGGAGATTGTTTCAGAAGATATTGAAGATTTTGAGTTCACCCCTAAGCCAGA ATATGAAGGGCCCTTTTGTGTCTTCAATGAACCCGACGAGGTCCATCTGATCCAGAGATGGTTTGAGCAT ATCCAGGAAACCAAACCTACCATTATGGTCACCTACAATGGGGATTTTTTTGACTGGCCATTTGTGGAGG CTAGGGCAGCAATTCATGGCCTCAGCATGTACCAGGAGATAGGCTTCCAGAAGGATAGCCAGGGGGAATA TAAGGCACCACAGTGCATCCACATGGACTGCCTCAGGTGGGTGAAGAGGGACAGTTACCTTCCTGTGGGC AGTCATAATCTCAAGGCAGCTGCCAAGGCCAAACTTGGCTATGACCCTGTAGAGCTGGACCCTGAGGACA TGTGTCGTATGGCCACTGAACAGCCCCAGACTCTGGCCACTTACTCAGTGTCAGATGCTGTGGCTACTTA CTACCTGTACATGAAATACGTCCACCCCTTCATATTCGCCCTGTGCACCATTATTCCCATGGAACCTGAT GAGGTGCTGCGGAAGGGCTCCGGGACACTGTGTGAAGCCTTGCTGATGGTGCAAGCTTTCCATGCCAACA TTATCTTCCCCAATAAGCAAGAGCAGGAGTTCAACAAGCTGACAGATGATGGCCACATGCTAGATGCTGA GACCTACGTCGGGGGCCACGTGGAGGCACTAGAGTCTGGTGTCTTCAGAAGTGATATCCCCTGCCGGTTT AGGATGAATCCTGCAGCCTTTGATTTCCTGCTGCAACGAGTCGAGAAGACTATGCGCCACGCCATTGAAG AAGAAGAGAAGGTGCCTGTGGAACAAGCCACCAACTTTCAAGAGGTGTGTGAGCAGATTAAGACCAAGCT CACCTCCCTAAAAGATGTTCCTAACAGAATTGAATGTCCTCTAATCTACCATCTAGATGTGGGGGCCATG TATCCTAACATAATTCTTACCAACCGCCTACAGCCTTCTGCCATAGTGGATGAGGCCACCTGTGCTGCCT GTGACTTCAATAAGCCTGGAGCAAGTTGTCAGAGGAAGATGGCCTGGCAGTGGAGGGGAGAATTCATGCC AGCCAGTCGCAGTGAATACCATCGGATTCAGCATCAGCTGGAGTCGGAGAAGTTTCCCCCTTTGTTTCCA GAGGGGCCAGCACGGGCCTTTCACGAGCTGTCCCGTGAAGAACAGGCTAAATACGAGAAGAGGAGGCTGG CAGATTATTGCCGGAAAGCCTATAAGAAGATCCATGTGACCAAGGTAGAAGAACGTCTAACTACCATCTG CCAGCGGGAAAACTCATTTTATGTGGACACAGTGCGGGCCTTCAGAGACAGGCGCTATGAGTTCAAAGGA CTGCACAAGGTGTGGAAGAAGAAGCTCTCGGCAGCTGTAGAGGTGGGCGATGCATCAGAGGTGAAGCGCT GCAAGAACATGGAGATCCTTTACGATTCACTGCAGCTGGCTCACAAGTGCATCCTGAACTCCTTCTACGG CTATGTCATGCGCAAAGGAGCTCGCTGGTATTCCATGGAGATGGCTGGTATCGTCTGCTTTACAGGAGCC AACATCATCACCCAAGCAAGAGAACTGATTGAGCAGATCGGGAGGCCTTTAGAATTGGACACGGACGGAA TATGGTGCGTCCTACCCAATAGCTTTCCTGAAAATTTTGTCATCAAGACAACCAATGCGAAGAAACCCAA ACTGACCATCTCCTATCCTGGTGCCATGTTGAACATCATGGTCAAGGAAGGCTTTACCAACCACCAGTAC CAGGAACTAACAGAGCCTTCGTCTCTCACCTATGTCACCCACTCTGAGAATAGTATCTTTTTTGAAGTCG ATGGACCATACCTTGCTATGATCCTTCCAGCCTCCAAGGAAGAAGGCAAGAAGCTGAAGAAAAGATATGC TGTGTTCAATGAAGATGGTTCCTTGGCTGAACTGAAAGGTTTTGAGGTGAAACGCCGAGGGGAGTTGCAG CTGATTAAAATATTCCAGTCCTCAGTTTTTGAGGCCTTCCTCAAGGGCAGCACACTGGAGGAAGTGTATG GCTCGGTGGCCAAAGTGGCTGACTACTGGCTAGATGTGCTCTATAGCAAGGCTGCTAATATGCCCGATTC TGAATTGTTTGAGCTGATTTCTGAGAACCGCTCCATGTCTCGGAAGCTGGAAGATTACGGGGAGCAGAAG TCTACATCCATCAGCACAGCAAAGCGCCTGGCTGAGTTCCTGGGAGACCAGATGGTCAAAGATGCTGGAC TGAGCTGCCGCTATATCATCTCCCGAAAGCCAGAGGGGTCTCCTGTCACTGAGAGGGCCATTCCACTTGC CATTTTCCAAGCAGAGCCTACAGTGAGGAAACATTTTCTCCGGAAATGGCTAAAGAGTTCATCACTTCAA GACTTTGATATTCGGACAATTCTGGACTGGGACTACTACATAGAGAGGCTGGGGAGTGCCATCCAGAAAA TCATCACCATCCCCGCAGCTCTGCAGCAGGTGAAGAACCCAGTTCCACGTGTCAAACATCCAGACTGGCT ACACAAAAAACTACTAGAGAAGAATGATATCTACAAACAGAAGAAGATCAGTGAGCTCTTTGTGCTTGAA GGAAAGAGACAGATTGTGATGGCCCAGGCTTCAGAAAACAGTCTGAGTCTCTGCACTCCAGACATGGAGG ACATTGGACTCACAAAGCCACACCACTCTACAGTCCCAGTTGCTACTAAGAGGAAGCGAGTCTGGGAGAC CCAAAAGGAGTCTCAGGATATTGCACTAACTGTGCCCTGGCAAGAGGTCTTAGGGCAGCCTCCCTCCCTT GGAACCACACAGGAAGAGTGGTTGGTCTGGCTCCAGTTCCACAAGAAAAAGTGGCAGCTGCAGGCCCAAC AGCGCCTAGCCCTCAGGAAGAAGCAACGCTTAGAGTCAGCAGAAGATATGCCAAGGCTTGGGCCTATCCG AGAGGGGCCTTCCACAGGACTGGGGAGCTTTTTGCGAAGGACTGCCCGCAGCATCATGGACCTTCCATGG CAGATAATACAGATCAGTGAGACCAGACAGGCTGGTCTGTTCCGGCTGTGGGCTATCATTGGCAATGACT TGCACTGCATCAAGCTGAGTATCCCTCGAGTATTCTATGTAAACCAGCGGGTTGCCAAAGCAGAGGATGG ACCTGCATATCGGAAGGTGAATCGGGCTCTTCCTCGTTCCAACATTGTCTACAATCTCTATGAGTATTCA GTACCAGAGGACATGTACCAAGAACACATCAACGAGATCAACACTGAGTTGTCAGTACCAGACATTGAGG GCGTGTATGAGACACAGGTCCCATTGTTATTCCGGGCCCTCGTGCAGCTGGGCTGTGTGTGTGTGGTCAA CAAGCAGCTGACAAGGCACCTTTCGGGCTGGGAAGCTGAAACTTTTGCCCTCGAGCACCTTGAAATGCGT TCTCTGGCCCAGTTCAGCTACTTGGAACCAGGGAGTATCCGCCATATCTACCTGTACCATCACACTCAGG GCCACAAGGCACTCTTTGGGGTCTTTATCCCCTCACAGCGAAGAGCATCTGTGTTTGTGTTGGATACTGT ACGAAGCAACCAAATGCCAGGGCTCAGTGCCCTGTACTCATCAGAACACAGCCTGCTGCTGGACAAGGTG GACCCCAAGCTCCTGCCTCCCCCAAAACACACCTTTGAAGTTCGTGCTGAAACCAACCTGAAGACTATCT GCAGAGCCATCCAGCGCTTCCTGCTTGCCTACAAGGAAGAGCGCCGAGGGCCCACACTCATCGCTGTCCA GTCTAGCTGGGAGCTGTGTAGGCTGACCAGTGAGATTCCAGTCTTAGAAGAGTTCCCACTAGTGCCTATC CGAGTGGCTGACAAGATCAGCTATGCAGTCCTGGACTGGCAGCGCCATGGAGCTCGCCGAATGATCCGGC ACTACCTCAATTTAGACTTGTGCCTGTCGCAGGCCTTTGAGATGAGCAGGTACTTCCACATCCCTGTTGG AAACCTGCCGGAAGACATCTCCACCTTTGGCTCAGACCTCTTTTTTGCACGCCACCTCCAGCACCATAAC CACCTGCTTTGGCTATCCCCTACCTCTCGGCCTGACCTGGGTGGGAAGGAAGCTGATGACAACCGCCTTG TCATGGAGTTTGATGACCGAGCCACTGTGGAGATCAATAGTTCTGGCTGTTACTCTACTGTGTGCGTGGA ACTGGACATTCAAAATCTGGCAGTCAACACCATCCTCCAGTCCCATCATGTCAATGACATGGAGGGGGCT GGCAGCATGGGCATCAGCTTCGATGTGATCCAGCAGGCCTCCCTAGAGGACATGGTAACAGGCAATCAAG CTGCCAGTGCCCTGGCCAACTACGATGAGACAGCCCTCTGCTCTAGTACCTTCAGGATCCTGAAGAGCAT GGTGGTTGGCTGGGTAAAGGAAATCACACAGTACCACAACATCTATGCTGACAACCAGGTAATGCACTTC TACCGCTGGCTCCAGTCACCGTGCTCTCTGCTCCACGACCCAGCCCTTCACCGGACGCTGCACAACATGA TGAAGAAGCTCTTCCTGCAGCTCATTGCTGAGTTCAAGCGCCTGGGGTCATCAGTCGTCTATGCCAACTT CAATCGCATCATTCTCTGTACAAAGAAGCGCCGAATAGAGGATGCCCTTGCCTATGTGGAATATATTACC AACAGCATCCATTCTAAAGAGATCTTCCATTCCCTGACCATCTCTTTCTCTCGATGCTGGGAATTCCTTC TCTGGATGGATCCATCCAACTATGGTGGAATCAAAGGAAAAGTTCCATCTAGTATTCACTGTGGACAGGT AAAAGAGCAAGACTCCCAGGCAAGAGAGGAAACTGATGAAGAGGAGGAGGACAAGGAAAAGGACGAGGAG GAAGAGGGCATGGGAGAGTCCGAGGTTGAGGACTTACTGGAGAACAACTGGAACATTCTACAGTTCTTGC CCCAGGCAGCCTCTTGCCAGAGCTACTTCCTCATGATTGTTTCAGCATACATCGTAGCTGTGTACCAAAG CATGAAGGAGGAGTTGAGACACAGTGCCCCGGGCAGTACCCCTGTGAAGAGGAAGGGGGCCAGCCAGTTC TCCCAGGAGTCTGAAGGGGCAACTGGATCTCTTCCTGGAATGATCACTTTCTCTCAAGATTATGTGGCAA ATGAGCTCACTCAGAGCTTCTTCACCATTACTCAGAAAATTCAGAAGAAAGTCACAGGTTCTCGGAACAC CACTGAGCCCTCAGAGATGTTCCCCGTCCTCCCTGGTTCACACTTGCTGCTCAATAATCCTGCTCTGGAG TTCATCAAATATGTGTGCAAGGTACTATCTCTGGATACAAACATCACAAATCAGGTGAATAAGCTGAACA GAGACCTTCTTCGCCTGGTAGACGTTGGTGAATTCTCTGAGGAGGCCCAGTTCAGAGACCCCTGCCACTC CTACGTGCTCCCTGAGGTAATCTGCCACAGCTGTAATTTCTGCCGAGACCTGGACCTGTGCAAAGATTCC TCTTTCTCTCAGGATGGAGCCATCCTGCCTCAGTGGCTCTGCTCCAATTGTCAAGCCCCCTATGACTCCT CTGCCATTGAGTCAGCCTTGGTGGAAGCCCTGCAGAGGAAACTGATGGCCTTCACACTTCAGGACCTGGT ATGCCTCAAGTGCCGTGGTATGAAAGAGACCCATATGCCTGTGTACTGCAGCTGCGCAGGGGACTTTACT CTCACCATCCGCACTGAGGTCTTCATGGAACAGATTAGAATCTTCCAGAACATTGCCAAGTACTACAGCA TGTCATATCTCCAGGAGACCATAGAATGGCTGTTACAGACAAGCCCTGTATCAAACTGTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_011132
Insert Size	6852 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_011132.2, NP_035262.2
RefSeq Size	7167 bp
RefSeq ORF	6852 bp
Locus ID	18973
UniProt ID	Q9WVF7
Gene Summary	Participates in DNA repair and in chromosomal DNA replication.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.