Arhgap21 (BC076629) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	5530401C11Rik; AA416458; ARHGAP10
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG212100 representing BC076629 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACACATTGGACTGGTCTGCCTGAGGAAGATGGTGACAAACTCAAGGCCTGTGGGGCGGCCTCTG CCTGTGAGGTGTCAAAAAACAAAGATGGAAAAGACCAAGGTGAACCTGTGTCACCGTCTGAAGATGAGCC ATTCTCCTGGCCAGGTCCCAAAACAGTCATGCTGAAGAGAACATCTCAAGGGTTTGGTTTCACATTACGA CACTTCATCGTTTATCCCCCAGAGTCTGCAATCCAGTTTTCATATAAGGATGAAGAGAATGGAAACAGAG GAGGAAAGCAAAGGAACCGATTGGAGCCGATGGATACCATATTTGTTAAACAAGTGAAGGAAGGAGGGCC GGCCTTCGAGGCTGGCTTATGCACAGGTGACAGAATTATAAAAGTCAATGGAGAAAGTGTCATAGGAAAG ACCTATTCCCAAGTAATTGCTCTCATTCAGAACAGTGACACCACCCTGGAGCTCAGTGTAATGCCCAAGG ATGAAGACATTCTCCAAGTGGCATATTCTCAAGATGCATACCTGAAAGGCAATGAAGCTTACAGCGGCAA TGCCCGTAATATACCTGAACCTCCACCAGTTTGCTATCCCTGGTTGCCATCGACTCCTTCAGCTACTGCA CAGCCGGTGGAAACATGCCCTCCTGATTCATTACCGAACAAACAGCAAACCAGTGCACCAGTCCTCACAC AACCTGGCAGGGCCTATAGAATGGAAATACAAGTGCCTCCGTCACCAACAGATGTTGCAAAGTCAAACAC AGCAGTGTGTGTTTGCAATGAGAGTGTGAGGACCGTCATTGTGCCTTCTGAGAAGGTGGTAGACTTGCTA GCCAATAGAAACAACCCTTCAGGTCCTTCACATAGAACTGAAGAGGTAAGGTATGGTGTAAATGAGCAGG CCTCTACAAAAGCAGCATCGAGAACAACGTCACCGGCATCAGTTCCCACTGCCCATCTGATTCATCAGAC CACAGGCTCCAGATCCTTAGAACCTTCTGGAATTTTACTTAAATCTGGAAATTACAGTGGACATTCAGAA GGAATCTCAAGCAGTAGATCACAAGCCGTGGATTCCCCTCCTGTGTCTGTCAATCACTACTCTGCAAACT CCCATCAGCACATAGACTGGAAAAATTATAAGACTTACAAAGAATACATTGATAATAGGAGATTGCACAT AGGTTGTCGGACAATCCAGGAAAGACTGGACAGCTTACGAGCTGCATCTCAGAGCGCAGCAGACTATAAC CAGGTGGTACCCACCCGCACTACTCTGCAGGTACGACGTCGAAGCACCTCTCATGACCGAGTGCCCCAGT CTGTTCAGATCCGGCAGCGCAGTGTATCTCAGGAAAGACTGGAAGATTCTGTGCTGATGAAGTATTGCCC AAGGAGCGCGTCTCAAGGAGCGCTGACCTCACCGCCTGTTAGCTTCAATAATCATAGAACTCGTTCGTGG GATTACATAGAGGGACAGACGGAAGCCACAGCCACTGTCAATTCTGAAAGTCAGATACCTGACTCAAATG GGGAACGAAAACAGACTTACAAGTGGAGTGGCTTCACTGAGCAGGATGATAGACGCGGTATTCACGAAAG ACCCAGGCAGCAAGAAATGCACAAGCCTTTCCGAGGCTCAAATTTGACTGTGGCTCCCGTTGTTAATTCT GATAACAGGCGCCTGGTTGGGAGAGGGGTAGGACCTGTCTCACAGTTTAAAAAAATCCCGCCGGACCTAA GACCACCACATTCCAACAGAAATTTTCCAACCACGACTGGGGTGTCACTTCAACGGGGTATTGCACAAGA CAGGTCCCCACTCGTGAAAGTCCGAAGTAATTCTCTGAAAGTGCCACCTCCACCTGTGTCAAAACCGTCC TTCAGCCAGCACTCACTGGCTTCCATGAAAGACCAAAGGCCTGTCAATCATCTGCATCAACACAGTGTGC TGAGTCAGCAGACGCAGTTCAGATCCGAAAGCACCTTCGAGCACCAGCTGGAGACTGAGGTATCCTCGTG TCTACCAGGAACTTCTGCCAAGACCAGTCCACAGCTCAGTGAGAATTTGGGAACATCAGACTTAGAACTA CCTGCCATCCCAAGGAATGGAGATATAAACTTACAGGAGGCTGAAATTCAGCAGCCAGATGTGCTAGATA ATAAAGAATCTGTCATTCTAAGAGAGAAACCTCAGTCTGGCCGCCAGACGCCACAGCCTTTAAGACATCA GTCTTACATCCTGGCAGTGAATGACCAGGAGACCGGGTCAGACACCACCTGCTGGCTGCCAAACGATGCA CGCAGAGAGGTCCATATAAAAAGAATGGAGGAGAGGAAAGCTTCAAGCACCAGCCCTCCTGGTGATTCCC TGGCATCCATCCCTTTTATAGATGAGCCAACCAGTCCTAGCATCGACCACGAGATTGCACATATTCCTGC ATCTGCTGTCATCTCCGCCTCTACTGCCCACGTCCCCTCCATTGCAACTGTTCCCCCCAGCCTCACGACC TCTGCTCCTTTGATTCGACGTCAGCTCTCCCACGACCAAGAATCTGTTGGACCTCCCAGCCTGGATGGCC AGCACAGCTCAAAGACAGAAAGATCCAAATCCTACGATGAAGGACTGGATGATTATAGAGAAGATGCAAA ACTATCCTTTAAGCACGTGTCTAGCCTGAAGGGAATCAAGATTACAGACAGCCAGAAGTCATCAGAAGAC TCTGGATCGAGAAAGGGTTCTTCCTCCGAGGTCTTCAGTGATGCAGCCCGAGAAGGCTGGCTCCAGTTCC GACCACTGGTCACCGATAAGGGCAAGAGAGTTGGTGGCAGTATTCGGCCATGGAAACAGATGTATGTTGT CCTTCGGGGCCACTCACTTTACTTGTACAAAGACAGAAGAGAGCAGACAACGCCATCAGAAGAAGAGCAG CCCATCAGTGTCAACGCTTGCCTGATAGACATCTCGTACAGTGAGACCAAGAGGCGGAATGTATTCCGAC TTACCACGTCTGACTGTGAATGCTTGTTCCAGGCTGAAGACAGAGATGATATGTTATCATGGATCAAGAC TATTCAGGAAAGTAGCAACCTAAATGAAGAGGACACTGGAGTTACCAACAGAGATCTAATTAGTCGAAGA ATCAAAGAGTATAACAGTCTGCTGAGCAAAACAGAACAGTTGCCAAAAACACCTCGCCAGAGTCTTAGCA TCAGGCAAACTTTGCTTGGTGCTAAATCAGAGCCAAAGACTCAAAGCCCACATTCTCCAAAGGAGGAGTC CGAGAGGAAGCTTCTCAGTAAAGATGATACCAGTCCCCCTAAAGACAAAGGTACTTGGAGAAGAGGCATT CCAAGCATCGTGAGGAAGACGTTTGAGAAAAAGCCGGCCGCCACAGGGACCTTTGGAGTTAGGCTGGATG ACTGCCCACCCGCCCACACCAACAGGTACATTCCATTAATCGTTGACATATGTTGCAAATTAGTTGAAGA AAGGGGTCTTGAATATACAGGTATTTACCGAGTTCCCGGCAACAACGCAGCCATTTCAAGCATGCAGGAA GAACTCAACAAGGGGATGGCAGACATTGACATACAAGATGATAAATGGCGAGATTTGAATGTGATAAGCA GTTTACTAAAATCCTTCTTCAGAAAGCTCCCTGAGCCTCTCTTTACGAATGACAAATATGCTGACTTCAT TGAGGCCAATCGCAAAGAGGACCCACTGGATCGGCTACGAACATTAAAGAGACTAATTCATGACCTGCCT GAGCATCATTTTGAGACGCTCAAGTTTCTCTCGGCTCATCTGAAGACAGTGGCAGAGAACTCAGAAAAGA ATAAGATGGAACCCAGAAACCTAGCGATCGTTTTTGGTCCAACTCTAGTGAGAACATCAGAAGACAACAT GACCCACATGGTCACGCACATGCCTGACCAGTATAAGATCGTAGAGACTCTCATCCAGCATCATGACTGG TTTTTCACAGAAGAAGGTGCCGAAGAGCCTCTTACAGCAGTGCAGGAGGAGAACACAGTAGACTCCCAGC CAGTACCAAACATAGATCATTTGCTCACCAACATTGGAAGGACAGGCGTGCTCCCTGGAGATGTATCAGA TTCAGCTACTAGTGACTCCGCAAAATCTAAGGGCTCCTGGGGATCCGGGAAGGATCAGTATAGCAGGGAA CTGCTTGTATCCTCCATCTTCGCAGCTGCCAGTCGCAAGAGGAAAAAGCCGAAAGAGAAAGCCCAGCCCA GCAGCTCTGAGGATGAGCTGGACAGTGTGTTCTTTAAGAAGGAGAATACGGAACAGAGTCACAGTGAGAT TAAAGAAGAGTCCAAAAGAGAAAGCGAGACATCAGGTAGCAAGCAGAGGGTCGTCGTTGCCAAAGAAAGT AACACTAAGAAAGACTCGGGTACAACGAAGGAGGAGAAGAAGATACCGTGGGAAGAACCACCCCCACCAC ACAGCTCCAAACGCAACAGGTCACCAACCCTCAGCTGTCGCCTGGCCATGCTGAAAGAGGGCCCCAGATC ACTTCTGACACAGAAGCCCCACTGTGAAGAGACAGGGTCTGACTCAGGCACTCTGCTTAGCACGTCTTCC CAGGCTTCGCTCTTGAGGTCCTCCACTAAAAAATCCACCAGTCCGGAAACCAAGCACAGTGAGTTTTTGT CCATTGCTGGTACCACCACCTCGGATTATTCTACCACATCATCCACTACCTACTTGACCAGCCTGGACTC CAGCCGCCTGAGCCCTGAGGTGCAGTCAGTGGCCGAGAGCAAGGGGGATGAGGCTGATGACGAGAGGAGC GAGCTGGTGAGTGAAGGACGGCCAGTGGAGACCGACAGTGAGAGTGAGTTCCCCGTGTTTCCCACCACCC TGACTTCAGACCGGCTGTTCCGAGGAAAATTCCAGGAGGTTGCCAGGGTGAGCCGCAGGAACTCCGAAGG CAGTGAGGCCAGCTGCACCGAGGGAAGCCTCACACCGAGTTTAGACAGCCGCAGGCAGCAGTTCAGCTCC CATAGACTGATCGAATGCGACACACTGTCCAGGAAGAAGTCTGCTCGCTTCAAGTCAGACAGTGGGAGCC CCGGAGACACCAGGACTGAGAAAGAAACTCCTGCCCTGGCCAAGATGTTTGACGTCATGAAGAAAGGCAA GTCGACAGGGAGCCTCCTCACACCCAGCAGAAGTGAATCCGAAAAGCAGGAGGCCACTTGGAAAACAAAA ATAGCAGATCGTTTAAAACTGAGACCCAGGGCACCCGCAGACGACATGTTTGGAGTTGGGAATCAAAAAC CTACTGCTGAGACAGCGAAAAGGAAAAACATCAAACGCAGACACACGCTTGGAGGCCACAGAGATGCTAC TGAGATCAGCGTCCTGAGTTTCTGGAAAGCTCATGAGCAGAGTGCGGACAAAGAGTCTGAACTTTCGGCT GTGAATCGGTTAAAACCCAAATGCTCAGCCCAGGACCTTTCCATCTCAGACTGGCTCGCCAGGGAGCGCG TCCGCACCAGTGCCTCCGACCTTAGCAGAGGCGAAGGCCTCGAGCCCCAGGCTGAGAGCCCCAGCGTGCT GGGAACACCCATCAGCACCCACTCACCACCGTCTCAGCAGCCCGAAGCAAGGGTTGCTGCTACCAGCACC TTGGCTTCAACCAGCCAGTCCCCTCTTTTCACACCACCACAGTCACCTGACCAAATAAACAGAGAAAGCT TCCAGAACATGAGCCAAAATGCTAGTTCTACAGCTAACATCCACCCCCACAAACAGTCTGAAAGTCCAGA CACTAAAGCAGAGACTCCTCCC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG212100 representing BC076629 Red=Cloning site Green=Tags(s) MATHWTGLPEEDGDKLKACGAASACEVSKNKDGKDQGEPVSPSEDEPFSWPGPKTVMLKRTSQGFGFTLR HFIVYPPESAIQFSYKDEENGNRGGKQRNRLEPMDTIFVKQVKEGGPAFEAGLCTGDRIIKVNGESVIGK TYSQVIALIQNSDTTLELSVMPKDEDILQVAYSQDAYLKGNEAYSGNARNIPEPPPVCYPWLPSTPSATA QPVETCPPDSLPNKQQTSAPVLTQPGRAYRMEIQVPPSPTDVAKSNTAVCVCNESVRTVIVPSEKVVDLL ANRNNPSGPSHRTEEVRYGVNEQASTKAASRTTSPASVPTAHLIHQTTGSRSLEPSGILLKSGNYSGHSE GISSSRSQAVDSPPVSVNHYSANSHQHIDWKNYKTYKEYIDNRRLHIGCRTIQERLDSLRAASQSAADYN QVVPTRTTLQVRRRSTSHDRVPQSVQIRQRSVSQERLEDSVLMKYCPRSASQGALTSPPVSFNNHRTRSW DYIEGQTEATATVNSESQIPDSNGERKQTYKWSGFTEQDDRRGIHERPRQQEMHKPFRGSNLTVAPVVNS DNRRLVGRGVGPVSQFKKIPPDLRPPHSNRNFPTTTGVSLQRGIAQDRSPLVKVRSNSLKVPPPPVSKPS FSQHSLASMKDQRPVNHLHQHSVLSQQTQFRSESTFEHQLETEVSSCLPGTSAKTSPQLSENLGTSDLEL PAIPRNGDINLQEAEIQQPDVLDNKESVILREKPQSGRQTPQPLRHQSYILAVNDQETGSDTTCWLPNDA RREVHIKRMEERKASSTSPPGDSLASIPFIDEPTSPSIDHEIAHIPASAVISASTAHVPSIATVPPSLTT SAPLIRRQLSHDQESVGPPSLDGQHSSKTERSKSYDEGLDDYREDAKLSFKHVSSLKGIKITDSQKSSED SGSRKGSSSEVFSDAAREGWLQFRPLVTDKGKRVGGSIRPWKQMYVVLRGHSLYLYKDRREQTTPSEEEQ PISVNACLIDISYSETKRRNVFRLTTSDCECLFQAEDRDDMLSWIKTIQESSNLNEEDTGVTNRDLISRR IKEYNSLLSKTEQLPKTPRQSLSIRQTLLGAKSEPKTQSPHSPKEESERKLLSKDDTSPPKDKGTWRRGI PSIVRKTFEKKPAATGTFGVRLDDCPPAHTNRYIPLIVDICCKLVEERGLEYTGIYRVPGNNAAISSMQE ELNKGMADIDIQDDKWRDLNVISSLLKSFFRKLPEPLFTNDKYADFIEANRKEDPLDRLRTLKRLIHDLP EHHFETLKFLSAHLKTVAENSEKNKMEPRNLAIVFGPTLVRTSEDNMTHMVTHMPDQYKIVETLIQHHDW FFTEEGAEEPLTAVQEENTVDSQPVPNIDHLLTNIGRTGVLPGDVSDSATSDSAKSKGSWGSGKDQYSRE LLVSSIFAAASRKRKKPKEKAQPSSSEDELDSVFFKKENTEQSHSEIKEESKRESETSGSKQRVVVAKES NTKKDSGTTKEEKKIPWEEPPPPHSSKRNRSPTLSCRLAMLKEGPRSLLTQKPHCEETGSDSGTLLSTSS QASLLRSSTKKSTSPETKHSEFLSIAGTTTSDYSTTSSTTYLTSLDSSRLSPEVQSVAESKGDEADDERS ELVSEGRPVETDSESEFPVFPTTLTSDRLFRGKFQEVARVSRRNSEGSEASCTEGSLTPSLDSRRQQFSS HRLIECDTLSRKKSARFKSDSGSPGDTRTEKETPALAKMFDVMKKGKSTGSLLTPSRSESEKQEATWKTK IADRLKLRPRAPADDMFGVGNQKPTAETAKRKNIKRRHTLGGHRDATEISVLSFWKAHEQSADKESELSA VNRLKPKCSAQDLSISDWLARERVRTSASDLSRGEGLEPQAESPSVLGTPISTHSPPSQQPEARVAATST LASTSQSPLFTPPQSPDQINRESFQNMSQNASSTANIHPHKQSESPDTKAETPP TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC076629
ORF Size	5834 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC076629, AAH76629
RefSeq Size	6944 bp
RefSeq ORF	5834 bp
Locus ID	71435
Gene Summary	Functions as a GTPase-activating protein (GAP) for RHOA and CDC42. Downstream partner of ARF1 which may control Golgi apparatus structure and function. Also required for CTNNA1 recruitment to adherens junctions (By similarity).[UniProtKB/Swiss-Prot Function]

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