Fmn2 (BC094606) Mouse Tagged ORF Clone
CAT#: MG212040
- TrueORF®
Fmn2 (tGFP-tagged) - Mouse formin 2 (cDNA clone MGC:106665 IMAGE:6840339). Note: ORF is codon optimized
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CNY 17,390.00
货期*
5周
规格
经常一起买 (4)
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AU024104 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG212040 representing BC094606, codon optimized.
Due to the complexity of BC094606, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTAATCAGGATGGGAAACTGAAGCGCTCTGCTGGCGACGCATCACACGAAGGCGGTGGAGCCGAGG ATGCTGCAGGCCCGAGGGACGCCGAGATCACTAAGAAGGCTAGCGGCTCAAAAAAAGCCCTTGGCAAACA CGGTAAGGGTGGCGGGGGGAGTGGGGAGACATCTAAGAAGAAGAGCAAGTCTGACTCCCGAGCTTCTGTC TTTTCCAATTTGCGAATTCGGAAAAACCTGACAAAGGGTAAAGGCGCTTGTGATAGCAGGGAGGACGTGC TCGACAGCCAGGCCCTGCCCATTGGAGAGCTGGACTCAGCACACTCAATCGTAACTAAAACACCTGACCT TAGCCTGTCAGCCGAAGAAACAGGCCTGAGCGATACCGAGTGCGCTGACCCGTTTGAGGTCATCCACCCC GGCGCCTCTCGACCAGCGGAAGCCGGAGTCGGGATACAAGCGACCGCGGAGGACCTCGAAACCGCTGCAG GCGCCCAGGACGGCCAGCGCACCAGCTCTGGTAGCGATACCGATATCTATTCCTTTCATTCAGCCACCGA GCAGGAGGACCTGCTGTCTGACATTCAGCAGGCCATTCGGCTTCAGCAGCAGCAGCAGCAAAAGCTGCTT CTTCAAGATTCTGAAGAGCCCGCTGCCCCCCCCACTGCCATAAGCCCCCAACCTGGGGCATTTCTGGGTC TGGATCAGTTTTTGCTTGGCCCTCGCTCCGAAGCGGAGAAGGACACCGTGCAGGCACTCCCCGTCAGACC AGATCTGCCAGAGACTACTAAGTCTCTTGTCCCTGAGCATCCACCTTCCAGCGGATCACATCTGACATCA GAGACTCCAGGCTACGCAACAGCTCCCAGTGCCGTTACTGATAGTCTGTCCTCCCCAGCATTCACCTTTC CTGAGGCCGGCCCAGGGGAAGGTGCCGCAGGTGTCCCCGTTGCCGGCACCGGTGATACAGATGAAGAATG TGAAGAGGACGCATTCGAGGATGCACCAAGGGGTTCCCCCGGCGAGGAATGGGTCCCCGAGGTGGAGGAA GCTTCACAACGGTTGGAGAAGGAACCCGAGGAGGGCATGCGCGAGAGCATTACTAGTGCTGTGGTGTCCC TCCCCGGGAGCCCTGCCCCTTCACCACGCTGCTTTAAACCGTACCCACTCATTACGCCGTGCTATATTAA GACAACTACACGCCAGTTGAGCTCTCCAAACCACAGTCCTAGCCAGTCACCAAATCAGTCCCCACGAATC AAGAAGCGACCTGATCCATCCGTGTCACGGTCTAGTCGGACCGCACTGGCATCCGCTGCGGCACCAGCAA AGAAACACCGCCTCGAAGGCGGTCTGACTGGTGGCCTCTCTAGAAGTGCAGACTGGACTGAAGAGCTGGG GGTGAGGACCCCAGGGGCCGGCGGCTCCGTCCATCTCTTGGGTCGGGGGGCAACCGCTGACGATAGTGGC GGCGGATCTCCCGTTCTGGCCGCAAAGGCGCCCGGGGCTCCAGCCACGGCTGACGGCTTCCAGAACGTGT TCACCGGTAGGACTCTGCTGGAGAAGCTGTTCAGCCAACAGGAGAACGGCCCACCTGAGGAGGCAGAGAA ATTTTGCTCACGGATTATTGCTATGGGCCTGCTGCTTCCTTTTTCCGACTGTTTTAGGGAGCCCTGTAAT CAGAATGCAGGCTCCTCTAGCGCACCGTTCGACCAGGATCAACTTTATACTTGGGCCGCCGTCTCTCAGC CAACCCACTCTATGGATTATTCCGAGGGACAGTTCCCCCGCCGGGAACCGTCAATGTGGCCCTCTAGCAA ATTGCCCGAAGAGGAGCCATCTCCCAAGGACGTAGACACGGAACCAAAGAGCTCTATCCTCGAATCCCCC AAGAAATGTAGCAACGGCGTGCAGCAGGAAGTGTTCGACGTTAAGTCCGAGGGCCAGGCTACCGTGATCC AGCAACTGGAGCAGACAATCGAGGACCTTCGCACCAAGATCGCTGAGCTGGAAAAACAGTATCCTGCCCT GGATCTGGAGGGGCCCCGGGGTCTTAGCGGCCTGGAGAACGGGCTCACAGCCTCCGCTGACGTGTCTCTG GATGCATTGGTGCTGCACGGGAAGGTCGCACAGCCTCCTAGAACCCTTGAAGCGAAATCTATCCAGACTT CTCCTACAGAGGAGGGGCGAATTCTTACACTTCCACCACCTAAAGCTCCTCCAGAAGGACTGCTGGGGTC ACCCGCGGCAGCCTCAGGCGAATCCGCGCTTCTGACGTCTCCCTCAGGGCCTCAAACTAAGTTCTGTTCC GAGATATCCCTGATAGTGAGCCCCAGGAGGATCTCAGTGCAGCTGGATGCACAGCAAATTCAATCAGCTT CCCAGCTGCCACCCCCGCCTCCTCTTCTGGGATCAGACTCCCAGGGTCAGCCATCACAGCCCAGTTTGCA TACGGAAAGCGAAACATCCCACGAGCATAGCGTATCCTCCAGCTTTGGGAACAACTGTAATGTGCCACCG GCCCCCCCACTGCCTTGTACAGAATCATCTAGTTTTATGCCCGGGCTCGGAATGGCAATTCCACCTCCGC CCTGCCTCTCCGACATAACCGTGCCTGCACTGCCTTCACCTACAGCCCCCGCTCTCCAGTTTTCAAATCT GCAGGGCCCTGAAATGCTGCCAGCGCCACCGCAGCCCCCCCCATTGCCAGGCCTGGGAGTACCACCACCA CCCCCGGCCCCACCCCTTCCAGGCATGGGTATTCCGCCTCCTCCACCCCTTCCGGGAATGGGCATCCCAC CTCCACCTCCTCTGCCAGGTATGGGAATTTCACCCTTGCCACCTCTACCTGGAATGGGAATCCCTCCACC TCCCCCACTGCCTGGAGTCGGTATTCCACCTCCACCGCCTCTTCCAGGAGTGGGAATCCCACCACCTCCA CCACTGCCTGGAGTGGGAATACCTCCACCTCCACCTCTGCCAGGTGTTGGCATCCCTCCACCGCCACCTC TGCCTGGAGTTGGCATTCCACCACCTCCGCCACTTCCAGGCGTAGGCATCCCTCCACCACCTCCGTTGCC TGGTGTGGGTATCCCACCACCTCCTCCACTCCCAGGTGTGGGTATACCACCACCGCCTCCTCTGCCAGGC AGCGGAATCCCTCCTCCTCCAGCTCTGCCAGGTGTTGCTATACCTCCACCACCTCCACTCCCCGGCATGG GTGTGCCTCCGCCAGCTCCTCCACCTCCTGGCGCTGGAATTCCACCCCCGCCCCTCTTGCCAGGCTCTGG GCCTCCCCATAGCAGTCAGGTCGGATCAAGCACTCTCCCAGCTGCCCCACAAGGATGCGGGTTTTTGTTC CCCCCACTGCCTACTGGGTTGTTCGGACTCGGCATGAACCAAGACAGGGTGGCGAGAAAGCAACTGATCG AACCATGTAGACCCATGAAGCCGCTGTATTGGACTCGCATTCAGCTGCACTCAAAGCGGGATTCTAGCCC CAGCCTGATTTGGGAAAAAATCGAGGAGCCCTCCATCGACTGTCACGAATTCGAAGAGCTGTTTTCAAAA ACCGCCGTTAAAGAAAGAAAGAAGCCCATCAGCGATACCATCTCTAAGACAAAGGCTAAGCAGGTCGTGA AGTTGTTGTCAAATAAGCGCTCCCAAGCCGTGGGGATCCTTATGAGCAGCCTGCATCTCGACATGAAAGA TATTCAGCACGCAGTAGTAAACCTGGACAACAGCGTCGTCGACTTGGAAACTCTTCAGGCCCTCTACGAG AACCGGGCCCAATCCGACGAACTGGAGAAAATCGAGAAGCACAGTAGGAGCAGCAAAGACAAGGAGAACG CAAAGAGTCTGGACAAACCTGAGCAATTCCTGTATGAGTTGAGTCTTATCCCAAACTTCTCTGAGCGGGT ATTCTGCATTCTTTTCCAGAGCACCTTCTCAGAATCTATCTGCTCTATAAGACGCAAACTGGAGCTCCTG CAGAAGCTCTGCGAAACTCTGAAGAACGGACCGGGGGTTATGCAGGTGCTTGGGCTTGTTCTGGCATTCG GCAATTATATGAACGCCGGCAACAAGACCAGAGGACAAGCTGATGGATTTGGTTTGGACATTCTTCCAAA ACTTAAAGACGTGAAGAGCTCAGATAACTCCAGATCCCTTCTGTCCTACATTGTCTCATATTACCTGAGA AACTTTGATGAAGATGCCGGCAAGGAACAGTGCGTGTTTCCTCTCGCCGAGCCACAGGAACTGTTCCAGG CCTCACAGATGAAGTTCGAGGATTTCCAGAAGGACCTGCGAAAGCTGAAGAAGGACTTGAAGGCGTGTGA GGCTGAGGCCGGTAAGGTGTACCAGGTCAGTAGTGCCGAGCACATGCAACCCTTCAAAGAGAACATGGAG CAGTTCATTTCCCAGGCAAAAATTGATCAAGAAAGCCAGGAGGCTGCCCTCACTGAGACTCACAAATGTT TTCTTGAAACAACCGCATACTACTTTATGAAGCCAAAATTGGGAGAGAAGGAAGTTAGCCCGAATGTCTT TTTCTCCGTCTGGCATGAGTTCTCATCCGACTTCAAAGACGCTTGGAAGAAGGAGAATAAACTGATCCTG CAAGAACGGGTCAAAGAGGCTGAAGAAGTGTGTCGCCAAAAGAAGGGGAAAAGCCTCTATAAAGTGAAGC CACGACACGATAGCGGCATTAAGGCTAAAATCAGTATGAAGACT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG212040 representing BC094606
Red=Cloning site Green=Tags(s) MGNQDGKLKRSAGDASHEGGGAEDAAGPRDAEITKKASGSKKALGKHGKGGGGSGETSKKKSKSDSRASV FSNLRIRKNLTKGKGACDSREDVLDSQALPIGELDSAHSIVTKTPDLSLSAEETGLSDTECADPFEVIHP GASRPAEAGVGIQATAEDLETAAGAQDGQRTSSGSDTDIYSFHSATEQEDLLSDIQQAIRLQQQQQQKLL LQDSEEPAAPPTAISPQPGAFLGLDQFLLGPRSEAEKDTVQALPVRPDLPETTKSLVPEHPPSSGSHLTS ETPGYATAPSAVTDSLSSPAFTFPEAGPGEGAAGVPVAGTGDTDEECEEDAFEDAPRGSPGEEWVPEVEE ASQRLEKEPEEGMRESITSAVVSLPGSPAPSPRCFKPYPLITPCYIKTTTRQLSSPNHSPSQSPNQSPRI KKRPDPSVSRSSRTALASAAAPAKKHRLEGGLTGGLSRSADWTEELGVRTPGAGGSVHLLGRGATADDSG GGSPVLAAKAPGAPATADGFQNVFTGRTLLEKLFSQQENGPPEEAEKFCSRIIAMGLLLPFSDCFREPCN QNAGSSSAPFDQDQLYTWAAVSQPTHSMDYSEGQFPRREPSMWPSSKLPEEEPSPKDVDTEPKSSILESP KKCSNGVQQEVFDVKSEGQATVIQQLEQTIEDLRTKIAELEKQYPALDLEGPRGLSGLENGLTASADVSL DALVLHGKVAQPPRTLEAKSIQTSPTEEGRILTLPPPKAPPEGLLGSPAAASGESALLTSPSGPQTKFCS EISLIVSPRRISVQLDAQQIQSASQLPPPPPLLGSDSQGQPSQPSLHTESETSHEHSVSSSFGNNCNVPP APPLPCTESSSFMPGLGMAIPPPPCLSDITVPALPSPTAPALQFSNLQGPEMLPAPPQPPPLPGLGVPPP PPAPPLPGMGIPPPPPLPGMGIPPPPPLPGMGISPLPPLPGMGIPPPPPLPGVGIPPPPPLPGVGIPPPP PLPGVGIPPPPPLPGVGIPPPPPLPGVGIPPPPPLPGVGIPPPPPLPGVGIPPPPPLPGVGIPPPPPLPG SGIPPPPALPGVAIPPPPPLPGMGVPPPAPPPPGAGIPPPPLLPGSGPPHSSQVGSSTLPAAPQGCGFLF PPLPTGLFGLGMNQDRVARKQLIEPCRPMKPLYWTRIQLHSKRDSSPSLIWEKIEEPSIDCHEFEELFSK TAVKERKKPISDTISKTKAKQVVKLLSNKRSQAVGILMSSLHLDMKDIQHAVVNLDNSVVDLETLQALYE NRAQSDELEKIEKHSRSSKDKENAKSLDKPEQFLYELSLIPNFSERVFCILFQSTFSESICSIRRKLELL QKLCETLKNGPGVMQVLGLVLAFGNYMNAGNKTRGQADGFGLDILPKLKDVKSSDNSRSLLSYIVSYYLR NFDEDAGKEQCVFPLAEPQELFQASQMKFEDFQKDLRKLKKDLKACEAEAGKVYQVSSAEHMQPFKENME QFISQAKIDQESQEAALTETHKCFLETTAYYFMKPKLGEKEVSPNVFFSVWHEFSSDFKDAWKKENKLIL QERVKEAEEVCRQKKGKSLYKVKPRHDSGIKAKISMKT TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | BC094606 |
ORF Size | 4734 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | BC094606, AAH94606 |
RefSeq Size | 5797 bp |
RefSeq ORF | 4736 bp |
Locus ID | 54418 |
Gene Summary | Actin-binding protein that is involved in actin cytoskeleton assembly and reorganization (PubMed:18848445, PubMed:21620703). Acts as an actin nucleation factor and promotes assembly of actin filaments together with SPIRE1 and SPIRE2 (PubMed:18848445, PubMed:21620703). Involved in intracellular vesicle transport along actin fibers, providing a novel link between actin cytoskeleton dynamics and intracellular transport (PubMed:21983562). Required for asymmetric spindle positioning, asymmetric oocyte division and polar body extrusion during female germ cell meiosis (PubMed:12447394, PubMed:18848445, PubMed:19062278, PubMed:21620703). Plays a role in responses to DNA damage, cellular stress and hypoxia by protecting CDKN1A against degradation, and thereby plays a role in stress-induced cell cycle arrest (By similarity). Also acts in the nucleus: together with SPIRE1 and SPIRE2, promotes assembly of nuclear actin filaments in response to DNA damage in order to facilitate movement of chromatin and repair factors after DNA damage (By similarity). Protects cells against apoptosis by protecting CDKN1A against degradation (By similarity).[UniProtKB/Swiss-Prot Function] |
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