Mast1 (BC054524) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	SAST, SAST170
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG212038 representing BC054524 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGACTCTCTCTGGACCGCGCTTTCCAATTTCTCGATGCCCTCCTTCCCCGGCGGCAGCATGTTTC GCCGCACCAAGAGTTGCCGTACCAGCAACCGGAAAAGCCTCATCCTGACAAGCACTTCTCCGACTCTACC GCGACCCCACTCCCCGCTGCCGGGCCACTTAGGTAGCAGTCCTTTGGACAGCCCCCGCAACTTCTCTCCC AACACTCCCGCCCACTTCTCGTTTGCTTCTTCCCGAAGGGCGGATGGACGCCGATGGTCGCTTGCTTCGC TCCCTTCCTCTGGCTATGGCACCAACACGCCCAGCTCAACTGTTTCATCCTCCTGCTCCTCCCAGGAGCG CCTGCATCAACTACCGTACCAGCCCACCGTGGACGAGCTGCACTTTCTGTCCAAACACTTCGGCAGCACC GAGAGCATCACCGACGAGGATGGTGGCCGCCGCTCTCCCGCCGTGCGTCCTCGCTCTCGCAGCCTCAGCC CCGGCCGCTCCCCCTCCTCCTACGACAACGAAATTGTGATGATGAACCATGTGTACAAGGAGCGGTTCCC AAAGGCCACTGCACAGATGGAAGAGAAGCTGCGGGACTTCGCCCGCGCCTATGAACCTGACAGCGTGCTG CCGCTGGCCGACGGCGTGCTCAGCTTCATCCACCATCAGATCATTGAACTGGCCCGAGACTGCCTTACCA AGTCCCGCGATGGGCTCATCACCACGGTCTATTTCTATGAACTGCAGGAAAACCTAGAGAAGCTGCTGCA GGACGCTTATGAACGCTCGGAGAGCTTGGAGGTAGCCTTTGTCACTCAGCTGGTGAAGAAACTTCTCATA ATCATCTCTCGCCCTGCAAGGCTTCTGGAGTGCCTGGAATTCAACCCAGAAGAGTTCTACCACCTTCTGG AGGCAGCCGAGGGACATGCCAAGGAGGGCCACCTGGTCAAAACTGACATCCCCCGATACATCATCCGACA GCTGGGCCTCACTCGGGACCCCTTTCCAGATGTGGTGCGCCTGGAGGAGCAGGACAGCGGGGGCTCCAAC ACTCCGGAGCAAGACGACACATCTGAGGGTCGCAGCAGCACCTCCAAGGCCAAGAAACCTCCCGGTGAAA GTGACTTTGACACTATCAAGCTCATTAGCAATGGTGCCTACGGAGCCGTCTACCTGGTGCGGCACCGGGA CACGAGGCAGCGCTTCGCCATGAAGAAGATCAACAAACAGAACCTGATCCTGCGCAACCAGATCCAGCAG GCGTTTGTGGAGCGCGACATCCTCACGTTTGCAGAGAACCCATTTGTGGTCGGCATGTTCTGCTCCTTTG AGACCCGGCGCCACCTTTGCATGGTCATGGAGTATGTGGAAGGTGGTGACTGTGCCACTCTCCTCAAGAA CATTGGGGCACTGCCTGTGGAGATGGCGCGAATGTATTTTGCAGAAACAGTTCTAGCCCTTGAGTACTTA CACAATTATGGCATCGTGCACCGTGACCTCAAGCCAGACAACCTCCTCATTACTTCCATGGGCCACATCA AGCTCACGGATTTTGGCCTCTCCAAGATGGGACTCATGAGCCTGACCACCAACTTATATGAGGGGCACAT TGAGAAGGATGCCCGGGAGTTCCTGGACAAACAGGTGTGTGGGACCCCAGAGTACATCGCACCCGAGGTC ATCCTGCGCCAGGGCTACGGCAAGCCAGTGGACTGGTGGGCTATGGGGATCATTCTCTACGAGTTCCTGG TGGGCTGTGTGCCTTTCTTCGGGGATACCCCAGAAGAGCTCTTTGGACAGGTCATCAGCGATGACATACT GTGGCCTGAAGGGGACGAGGCCCTGCCCACAGATGCACAGCTTCTAATATCTAGCCTCTTGCAGACCAAC CCCCTGGTCCGGCTTGGGGCAGGTGGTGCTTTTGAGGTGAAGCAGCATAGTTTCTTCCGAGACCTGGACT GGACAGGGCTACTGCGGCAGAAGGCGGAGTTCATCCCCCACCTAGAGTCAGAAGATGACACCAGCTACTT TGACACCCGCTCCGACAGGTATCACCATGTGAACTCCTATGATGAGGACGACACCACCGAAGAGGAGCCC GTGGAGATCCGCCAATTCTCCTCCTGCTCGCCGCGCTTCAGCAAGGTGTACAGCAGTATGGAGCAGCTGT CTCAGCACGAGCCTAAGACCCCCGTCTCAGCATCTGGGGCCAGCAAGCGGGACCCAAGCGCTAAGGGCCC GGAGGAGAAAGTGGCCGGCAAGAGGGAGGGACTGGGTGGCCTGACTCTGCGTGAGAAGACCTGGAGAGGG GGCTCTCCAGAGATTAAGCGCTTCTCAGCGTCTGAAGCCAGTTTTCTGGAAGGAGAGGCCAGTCCCCCGC TGGGAGCGCGCAGACGTTTCTCAGCACTGCTGGAGCCCAGCCGCTTCACTGCCCCTCAAGAGGATGAAGA TGAGGCTCGACTCCGCAGACCTCCTCGACCCAGCTCCGATCCCCCTAGCTCACTGGACACACGCGTCCCC AAAGAGGCAGTTCAAGGAGAGGGCACGTCCACCCCCGGGGAACCCGAGGCCACTGAGCGCTCACATCCAG GTGACCTCGGCCCACCCTCCAAGGATGGAGACCCATCAGGCCCTAGGGCCACCAACGACCTGGTTTTGCG AAGGGCAAGGCACCAGCAGCTGTCTGGGGATCTTGCTGTGGAGAAGCGACCGTCTCGAACTGGGGGCAAA GTTATCAAGTCGGCTTCAGCTACAGCCTTGTCAGTCATGATTCCTGCAGTGGACCCACATGGAGGCTCAC CCCTGGCTAGTCCCATGTCCCCTCGATCTCTCTCCTCCAATCCATCCTCACGGGACTCTTCGCCTAGCCG AGACTACTCACCAGCTGTCAGTGGACTCCGTTCCCCCATCACCATCCAACGCTCAGGCAAGAAATATGGG TTCACACTGCGAGCCATCCGTGTCTACATGGGCGACTCCGATGTCTACAGTGTCCACCACATTGTTTGGC ACGTGGAGGAAGGAGGCCCAGCCCAAGAGGCAGGGCTCTGTGCTGGGGACCTCATCACTCACGTGAATGG TGAGCCCGTGCATGGTATGGTGCATCCTGAGGTGGTAGAGCTGATCCTGAAGAGTGGCAACAAAGTAGCT GTGACCACCACACCCTTTGAAAACACTTCCATCCGGATTGGGCCTGCCAGGCGCAGCAGTTACAAAGCCA AAATGGCCCGAAGGAACAAAAGGCCTTCGGCCAAAGACGGCCAGGAGAGCAAGAAGCGGAGCTCTCTCTT TCGGAAGATCACAAAGCAGTCGAACCTGCTGCACACCAGTCGATCGCTGTCCTCTTTGAACCGGTCCTTG TCGTCCAGCGACAGTCTTCCGGGCTCGCCCACGCACGGACTACCCGCACGCTCGCCCACACATAGCTATC GCTCTACACCTGACTCCGCCTACTTAGGCGCCTCGTCACAGAGTAGCTCGCCAGCTTCCAGCACGCCTAA TTCCCCCGCCTCATCCGCCTCGCACCACATCAGGCCCAGCACCTTGCACGGGCTGTCACCGAAACTACAC CGCCAGTACCGCTCAGCGCGCTGTAAATCGGCTGGCAACATCCCGCTGTCACCTCTAGCGCACACACCAT CCCCGACGCAGGCGTCGCCGCCGCCGCTGCCAGGCCACACGGTGGGCAGCTCACATACCACGCAGAGCTT CCCTGCCAAACTACACTCATCGCCACCTGTCGTGCGCCCGCGCCCCAAAAGTGCAGAGCCACCACGCTCG CCGTTGCTCAAGCGCGTGCAGTCAGCGGAGAAACTAGGGGCTTCGCTGGGAGCAGACAAGAAGGGCGCAC TGCGCAAACACAGCCTGGAAGTGGGACACCCTGATTTCCGAAAGGACTTCCATGGTGAGCTGGCCCTGCA TAGCCTCGCTGAGTCTGATGGTGAGACGCCTCCTATTGAGGGTCCTGGGGCGACCCGGCAGGTCGCCGTG CGACGCCTGGGTCGCCAGGAATCACCCCTAAGCTTGGGCGCGGACCCGTTGCTACCCGATGGTGTCCAGA GACCTATGGCGTCAAGCAAGGAGGATTCCGCGGGAGGTACAGAGGCTTGCACCCCACCTCGCGCGACAAC CCCTGGGAGCCGGACCCTGGAGCGTGACTCGGGTTGCACAAGGCACCAGAGCGTGCAGACTGAGGATGGC CCAGGTGGGGTGGCCAGGGCCCTGGCCAAGGCAGCGCTAAGTCCAGTGCAGGAACATGAGACAGGCCGGC GCAGTAGCTCTGGTGAGGCAGGCACACCCCCGGTGCCCATTGTTGTAGAGCCTGCTCGGCCAGGGGTCAA GACCCAGGCACCCCAGCCTCTGGGCACAGACTCCAAGGGATTGAAGGAACCTGTAGCCCAGATGCCTCTG ATGCCTGATGCCCCAAGGGGCAGGGAGCGCTGGGTGCTAGAGGAGGTGGAAGAGCGGACCACGCTAAGCG GACTTCGCTCTAAGCCCGCCTCCCCCAAACTCTCTCCTGACCCCCAGACTCCAACCCTAGTCCCAACAAA GAATGTACCCCGCAGTGCAGCTCCTTCAGTCCCACCTGCTTCCCTCATGGTCCCAGGCACCAAGCCTGAA GCAGGGTTGAACTCCCGGTGTCCTGCTGAAGCTGTGACTCCTGCAGGCCTCACCAAAACTGGGGCACCTA GCCCTGCATCTCTAGGTCCA AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG212038 representing BC054524 Red=Cloning site Green=Tags(s) MSDSLWTALSNFSMPSFPGGSMFRRTKSCRTSNRKSLILTSTSPTLPRPHSPLPGHLGSSPLDSPRNFSP NTPAHFSFASSRRADGRRWSLASLPSSGYGTNTPSSTVSSSCSSQERLHQLPYQPTVDELHFLSKHFGST ESITDEDGGRRSPAVRPRSRSLSPGRSPSSYDNEIVMMNHVYKERFPKATAQMEEKLRDFARAYEPDSVL PLADGVLSFIHHQIIELARDCLTKSRDGLITTVYFYELQENLEKLLQDAYERSESLEVAFVTQLVKKLLI IISRPARLLECLEFNPEEFYHLLEAAEGHAKEGHLVKTDIPRYIIRQLGLTRDPFPDVVRLEEQDSGGSN TPEQDDTSEGRSSTSKAKKPPGESDFDTIKLISNGAYGAVYLVRHRDTRQRFAMKKINKQNLILRNQIQQ AFVERDILTFAENPFVVGMFCSFETRRHLCMVMEYVEGGDCATLLKNIGALPVEMARMYFAETVLALEYL HNYGIVHRDLKPDNLLITSMGHIKLTDFGLSKMGLMSLTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEV ILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDDILWPEGDEALPTDAQLLISSLLQTN PLVRLGAGGAFEVKQHSFFRDLDWTGLLRQKAEFIPHLESEDDTSYFDTRSDRYHHVNSYDEDDTTEEEP VEIRQFSSCSPRFSKVYSSMEQLSQHEPKTPVSASGASKRDPSAKGPEEKVAGKREGLGGLTLREKTWRG GSPEIKRFSASEASFLEGEASPPLGARRRFSALLEPSRFTAPQEDEDEARLRRPPRPSSDPPSSLDTRVP KEAVQGEGTSTPGEPEATERSHPGDLGPPSKDGDPSGPRATNDLVLRRARHQQLSGDLAVEKRPSRTGGK VIKSASATALSVMIPAVDPHGGSPLASPMSPRSLSSNPSSRDSSPSRDYSPAVSGLRSPITIQRSGKKYG FTLRAIRVYMGDSDVYSVHHIVWHVEEGGPAQEAGLCAGDLITHVNGEPVHGMVHPEVVELILKSGNKVA VTTTPFENTSIRIGPARRSSYKAKMARRNKRPSAKDGQESKKRSSLFRKITKQSNLLHTSRSLSSLNRSL SSSDSLPGSPTHGLPARSPTHSYRSTPDSAYLGASSQSSSPASSTPNSPASSASHHIRPSTLHGLSPKLH RQYRSARCKSAGNIPLSPLAHTPSPTQASPPPLPGHTVGSSHTTQSFPAKLHSSPPVVRPRPKSAEPPRS PLLKRVQSAEKLGASLGADKKGALRKHSLEVGHPDFRKDFHGELALHSLAESDGETPPIEGPGATRQVAV RRLGRQESPLSLGADPLLPDGVQRPMASSKEDSAGGTEACTPPRATTPGSRTLERDSGCTRHQSVQTEDG PGGVARALAKAALSPVQEHETGRRSSSGEAGTPPVPIVVEPARPGVKTQAPQPLGTDSKGLKEPVAQMPL MPDAPRGRERWVLEEVEERTTLSGLRSKPASPKLSPDPQTPTLVPTKNVPRSAAPSVPPASLMVPGTKPE AGLNSRCPAEAVTPAGLTKTGAPSPASLGP SGPTRTRRLE - GFP Tag - V
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	BC054524
ORF Size	4710 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC054524, AAH54524
RefSeq Size	4894 bp
RefSeq ORF	4712 bp
Locus ID	56527
Gene Summary	Appears to link the dystrophin/utrophin network with microtubule filaments via the syntrophins. Phosphorylation of DMD or UTRN may modulate their affinities for associated proteins.[UniProtKB/Swiss-Prot Function]

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