Kif21a (BC062896) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG212035 representing BC062896 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTGGGCGCTGCGGACGAGAGCTCGGTGCGCGTGGCTGTCAGAATAAGACCACAGCTTGCCAAAGAGA AGATCGAAGGTTGCCATATCTGCACGTCAGTCACACCGGGAGAGCCTCAGGTCTTCCTCGGGAAGGATAA GGCCTTTACTTTTGATTATGTGTTTGACATTGACTCCCAGCAGGAGCAGATCTACACCCAGTGCATCGAA AAGCTCATTGAAGGCTGTTTTGAAGGCTACAATGCCACCGTGTTTGCCTATGGACAAACCGGAGCTGGGA AAACCTACACGATGGGAACCGGATTTGACGTGAACATCATGGAGGAAGAGCAGGGCATCATCTCTCGTGC TGTTAGACACCTGTTCAAGAGTATTGATGAGAAAAAGACCTCAGCGATTAAAAACGGGCTGCCCCCTCCT GAATTCAAAGTGAATGCCCAGTTCCTAGAGCTCTATAATGAAGAGGTCCTTGACTTGTTTGATACCACTC GGGATATTGATGCAAAAAATAAAAAATCAAATATAAGAATTCATGAAGATTCAACTGGAGGAATTTATAC TGTGGGCGTCACAACACGCACTGTGAATACAGAACCGGAGATGATGCAGTGTCTGAAGCTGGGCGCTCTC TCGCGCACCACGGCCAGCACCCAGATGAACGTACAGAGCTCTCGTTCACACGCCATCTTTACCATTCACG TGTGTCAAACCAGAGTGTGTCCCCAAACAGATGCTGAGAACGCAACTGATAATAAGCTGATCTCCGAATC GTCGCCAATGAATGAGTTTGAGACGCTGACGGCGAAGTTTCACTTTGTTGATCTGGCGGGATCGGAAAGA CTGAAGAGAACCGGAGCTACAGGCGAGAGAGCAAAAGAGGGCATTTCCATCAACTGCGGGCTTTTGGCTC TTGGGAATGTAATCAGCGCCTTGGGGGACAAGAGCAAGAGAGCCACCCATGTCCCTTACAGAGACTCTAA GCTGACAAGACTCTTGCAGGATTCCCTTGGGGGTAACAGCCAAACCATCATGATAGCATGCGTCAGCCCT TCAGACAGGGATTTCATGGAGACGTTAAACACTCTGAAGTACGCCAATCGAGCGAGAAATATCAAGAACA AGGTGATGGTCAATCAAGACAGAGCCAGTCAGCAAATCAACGCGCTGCGGAGCGAGATCACGCGCCTTCA GATGGAGCTCATGGAATACAAAACCGGTAAAAGAATAATTGACGAGGAAGGCGTGGAAAGCATCAATGAC ATGTTTCATGAGAATGCTATGCTGCAGACGGAAAATAATAATCTGCGTGTAAGAATAAAAGCCATGCAGG AGACCGTTGATGCACTGAGGGCCAGAATCACGCAGCTTGTCAGTGAGCAGGCCAACCAAGTTCTTGCCCG AGCAGGTGAAGGGAACGAAGAGATCAGTAATATGATTCATAGTTATATCAAAGAAATTGAAGACCTCAGG GCAAAATTATTAGAAAGTGAAGCAGTGAACGAGAACCTTCGGAAGAACTTGACCAGAGCCACGGCGAGAT CTCCTTACTTCAGTGCCTCCTCAGCTTTCTCGCCTACTATACTGTCTTCAGACAAGGAGACTATCGAAAT TATAGATCTAGCAAAGAAAGACTTGGAGAAGCTAAAACGGAAAGAGAAGAAGAAGAAGAAAAGTGTTGCC GGGAAAGACGATAATGCAGACACTGACCAGGAGAAGAAAGAAGAAAAGGGTGTTTCAGAGAAAGAAAACA ATGAGCTAGACGTGGAAGAGAATCAAGAAGTGAGTGACCACGAGGATGAGGAAGAGGAGGAAGAGGACGA GGAGGAAGAGGATGACATTGAAGGAGAAGAAAGCTCTGATGAATCAGATTCCGAATCTGATGAAAAAGCT AACTATCAAGCCGACTTAGCAAATATCACCTGCGAGATTGCGATTAAGCAGAAGCTGATCGACGAACTGG AGAACAGCCAGAAACGGCTGCAGACCCTGAAAAAGCAGTACGAGGAGAAGCTGATGATGCTCCAACATAA GATCCGGGACACGCAGCTGGAGAGGGACCAGGTGCTCCAGAACCTAGGCTCGGTGGAGTCATACTCGGAA GAAAAGGCCAAGAAAGTGAAGTGCGAATATGAGAAGAAGCTCCACGCCATGAACAAAGAGCTGCAGCGAC TGCAGACGGCCCAAAAGGAGCACGCCAGGCTCCTCAAAAACCAGTCTCAGTATGAAAAGCAGCTCAAGAA ACTGCAGCAAGACGTCATGGAAATGAAGAAAACCAAGGTTCGTCTAATGAAGCAGATGAAAGAAGAGCAG GAGAAAGCCCGGCTGACAGAATCTCGAAGGAACCGGGAAATCGCTCAGCTGAAGAAGGATCAGCGCAAGC GAGATCATCAACTTAGACTTCTAGAGGCCCAGAAAAGAAATCAAGAAGTAGTTCTGCGACGCAAAACGGA AGAGGTTACAGCTCTCCGCAGGCAAGTGAGGCCCATGTCTGATAAAGTAGCGGGAAAAGTTACTCGGAAG CTGAGCTCATCCGAAAGCCCCGCTCCGGACACAGGTTCCAGTGCGGCTTCCGGGGAAGCAGACACATCAC GGCCAGGCACCCAGCAGAAAATGAGGATCCCCGTGGCAAGAGTCCAGGCATTACCAACACCTACAACAAA TGGCACCAGGAAAAAATATCAGAGGAAAGGATTCACTGGCCGGGTGTTCACTTCCAAGACAGCCCGCATG AAGTGGCAGCTACTGGAGCGCCGGGTGACCGACATCATCATGCAGAAAATGACCATCTCCAACATGGAGG CGGACATGAACAGACTCCTCAGGCAACGGGAAGAACTCACAAAAAGGCGAGAGAAACTTTCTAAAAGGAG AGAGAAGATAGTCAAGGAGAGCGGAGAGGGAGATAAAAGTGTGGCTAACATCATCGAGGAGATGGAGTCC CTGACAGCCAACATAGATTACATCAATGATAGCATTGCCGACTGTCAAGCCAACATCATGCAGATGGAGG AGGCAAAGGAAGAAGGGGAGACATTGGATGTCACCGCTGTCATTAATGCCTGTACACTGACAGAAGCTCG GTACCTGCTAGATCACTTCCTGTCAATGGGCATCAATAAGGGTCTGCAGGCTGCCCAGAAAGAGGCTCAA ATTAAAGTCCTCGAGGGTCGACTCAAACAGACCGAAATCACCAGTGCAACCCAGAACCAACTCTTATTCC ATATGCTGAAGGAGAAGGCAGAGCTAAACCCAGAGCTGGATGCCTTGCTAGGCCACGCACTGCAAGATCT AGATGGCGCCCCACCAGAAAACGAGGAGGACAGCAGCGAGGAAGATGGCCCTTTACACAGCCCGGGGTCG GAGGGAAGCACGTTATCTTCAGACCTTATGAAGCTTTGTGGTGAAGTGAAACCAAAGAACAAGGCTCGAA GGAGAACCACCACTCAGATGGAGTTGCTGTATGCAGATAGCAGTGAAGTAGCCTCAGACACTAGTGCAGG AGATGCCTCCTTGTCTGGCCCTCTGGCACCTGTTGCAGAAGGGCAGGAGATTGGAATGAACACAGAGACA AGTGGTACTTCTGCTAGGGACAAAGAGCTTCTTGCCCCATCTGGCTTACCTTCTAAGATAGGCAGCATAG GCATAATCAACCCATTTCCTGCCTGCAAAGGAGTCAGAGCCTCTCCTCTTCAGTGCGTTCACATAGCTGA AGGGCACACCAAGGCTGTGCTCTGTGTGGATTCTACGGATGATCTTCTCTTCACCGGATCAAAAGATCGC ACTTGTAAAGTATGGAATCTCGTGACTGGACAGGAAATTATGTCCCTGGGGGTTCACCCTAACAACGTGG TGTCTGTAAAATACTGTAATTATACCAGTCTGGTCTTCACCGTGTCGACATCTTATATTAAGGTGTGGGA TATCAGAGAGTCAGCAAAATGCATTCGAACATTAACATCTTCAGGTCAAGTTACCCTTGGAGAAGCGTGT TCTGCCAGCACCAGCCGGACAGTAGCTATTCCTTCCGGGGAGAGCCAGATCAATCAAATTGCACTAAACC CAACTGGCACTTTCCTCTACGCGGCCTCTGGAAATGCCGTCAGAATGTGGGACCTTAAAAGGTTTCAGTC TACAGGAAAGCTAACCGGACACCTGGGTCCTGTTATGTGCCTTACGGTAGACCAGATCTCCAATGGACAG GACCTCATCATCACTGGCTCAAAAGACCACTACATCAAAATGTTCGATGTGACTGAAGGGGCTCTTGGAA CTGTAAGTCCCACCCACAACTTCGAGCCTCCTCATTATGATGGGATAGAAGCACTGGCCATTCAAGGCGA TAACCTATTCAGTGGGTCCAGAGATAATGGAATCAAGAAATGGGACTTAGCTCAGAAAGGTCTTCTTCAG CAAGTCCCAAATGCACACAAAGACTGGGTGTGTGCCCTGGGCCTGGTGCCAGGCCATCCGGTTTTGCTGA GTGGCTGCAGAGGCGGCATTCTGAAACTCTGGAATGTGGACACTTTTGTGCCCGTTGGAGAGATGAGAGG TCATGACAGTCCCATCAATGCCATTTGTGTTAACTCCACCCATGTCTTTACTGCTGCTGATGATCGAACC GTGAGAATCTGGAAGGCCCACAACTTGCAAGATGGTCAACTCTCTGACACCGGCGATCTGGGGGAGGATA TTGCCAGTAAT ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG212035 representing BC062896 Red=Cloning site Green=Tags(s) MLGAADESSVRVAVRIRPQLAKEKIEGCHICTSVTPGEPQVFLGKDKAFTFDYVFDIDSQQEQIYTQCIE KLIEGCFEGYNATVFAYGQTGAGKTYTMGTGFDVNIMEEEQGIISRAVRHLFKSIDEKKTSAIKNGLPPP EFKVNAQFLELYNEEVLDLFDTTRDIDAKNKKSNIRIHEDSTGGIYTVGVTTRTVNTEPEMMQCLKLGAL SRTTASTQMNVQSSRSHAIFTIHVCQTRVCPQTDAENATDNKLISESSPMNEFETLTAKFHFVDLAGSER LKRTGATGERAKEGISINCGLLALGNVISALGDKSKRATHVPYRDSKLTRLLQDSLGGNSQTIMIACVSP SDRDFMETLNTLKYANRARNIKNKVMVNQDRASQQINALRSEITRLQMELMEYKTGKRIIDEEGVESIND MFHENAMLQTENNNLRVRIKAMQETVDALRARITQLVSEQANQVLARAGEGNEEISNMIHSYIKEIEDLR AKLLESEAVNENLRKNLTRATARSPYFSASSAFSPTILSSDKETIEIIDLAKKDLEKLKRKEKKKKKSVA GKDDNADTDQEKKEEKGVSEKENNELDVEENQEVSDHEDEEEEEEDEEEEDDIEGEESSDESDSESDEKA NYQADLANITCEIAIKQKLIDELENSQKRLQTLKKQYEEKLMMLQHKIRDTQLERDQVLQNLGSVESYSE EKAKKVKCEYEKKLHAMNKELQRLQTAQKEHARLLKNQSQYEKQLKKLQQDVMEMKKTKVRLMKQMKEEQ EKARLTESRRNREIAQLKKDQRKRDHQLRLLEAQKRNQEVVLRRKTEEVTALRRQVRPMSDKVAGKVTRK LSSSESPAPDTGSSAASGEADTSRPGTQQKMRIPVARVQALPTPTTNGTRKKYQRKGFTGRVFTSKTARM KWQLLERRVTDIIMQKMTISNMEADMNRLLRQREELTKRREKLSKRREKIVKESGEGDKSVANIIEEMES LTANIDYINDSIADCQANIMQMEEAKEEGETLDVTAVINACTLTEARYLLDHFLSMGINKGLQAAQKEAQ IKVLEGRLKQTEITSATQNQLLFHMLKEKAELNPELDALLGHALQDLDGAPPENEEDSSEEDGPLHSPGS EGSTLSSDLMKLCGEVKPKNKARRRTTTQMELLYADSSEVASDTSAGDASLSGPLAPVAEGQEIGMNTET SGTSARDKELLAPSGLPSKIGSIGIINPFPACKGVRASPLQCVHIAEGHTKAVLCVDSTDDLLFTGSKDR TCKVWNLVTGQEIMSLGVHPNNVVSVKYCNYTSLVFTVSTSYIKVWDIRESAKCIRTLTSSGQVTLGEAC SASTSRTVAIPSGESQINQIALNPTGTFLYAASGNAVRMWDLKRFQSTGKLTGHLGPVMCLTVDQISNGQ DLIITGSKDHYIKMFDVTEGALGTVSPTHNFEPPHYDGIEALAIQGDNLFSGSRDNGIKKWDLAQKGLLQ QVPNAHKDWVCALGLVPGHPVLLSGCRGGILKLWNVDTFVPVGEMRGHDSPINAICVNSTHVFTAADDRT VRIWKAHNLQDGQLSDTGDLGEDIASN TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC062896
ORF Size	4703 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC062896, AAH62896
RefSeq Size	6006 bp
RefSeq ORF	4703 bp
Locus ID	16564
Gene Summary	Microtubule-binding motor protein probably involved in neuronal axonal transport. In vitro, has a plus-end directed motor activity.[UniProtKB/Swiss-Prot Function]

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