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Nup214 (BC039282) Mouse Tagged ORF Clone
CAT＃: MG212020
- TrueORF®
Nup214 (tGFP-tagged) - Mouse nucleoporin 214 (cDNA clone MGC:37347 IMAGE:4976266)
ORF Plasmid: DDK
"BC039282" in other vectors (4)
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CN¥ 16,820.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | CAN, RP23-55I14.4 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG212020 representing BC039282
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCAGTACCCCAAACACAGGGATGAAGCCTTCGTTCCCTCCCTCGGCCTCTTCGGTGAAAGTCAACC TGAATGAGAAGTTCACCGCTGTGGCCTCCTCTGCACCTGTTCATAGCTCCACGAGCACACCCTCCGTGTT GCCGTTCTCCTCCTCCCCGAAGCCAACGGCTTCTGGACCACTCAGCCATCCTACGCCTCTCCCAGCATCA TCCAGCTCCATGCCTTTGAAGTCTTCGGTCTCTCCCTCGCCAGCAGCTGGCCGCTCCACTCAGACTGCGC CAAGTTCAGCACCCTCCACAGGGCAGAAATCACCCAGGGTAAACCCTCCAGTGCCAAAGTCAGGTTCTTC TCAGGCCAAGGCACTTCAGCCTCCTGTCACAGAGAAACAGAGGCCTCAGTGGAAAGACTCAGACCCCGTG CTGGCTGGGATTGGAGAGGAGATTGCACACTTCCAGAAAGAACTAGAGGAGTTAAAGGCCCGGACCGCCA AAGCCTGTCTCCAGGTTGGCACCTCAGAAGAGATGAAGATGCTGCGGACAGAGTCAGATGACTTGCACAC CTTTCTTTTTGAGATTAGAGAGACCACAGAGTCTCTTCATGGAGACATAAGTACTCTGAAAACAACTTTG CTGGAGGGCTTTGCTGGGGTCGAAGAAGCCAGAGAACAGCACGGAAGAAATCACGACTCTGGGTATCTCC ACCTGCTTTATAAAAGACCGCTGGATCCCAAGAGCGAGGCCCAGCTCCAGGAAATTCGCCGCCTTCACCA GTACGTGAAATTTGCTGTCCAGGATGTGAATGACGTCCTAGACTTAGAGTGGGATCGGCATCTGGAGCAG AAGAAGAGACAGAGGCGCCTAATTGTGCCAGAACGAGAGACACTTTTCAACACGCTAGCCAACAACCGGG AGATCATCAACCAGCAGAGGAAGAGGCTGAATCAGCTGGTGGACAGTCTGCAGCAGCTCCGTCTCTATAA CCACACGGCCCCGTGGAGCCTGCCCTCAGCGCTCTCAACTCAGAGCAACAGTCACAGTTTTGACAGTGAC CTTGAATGCCTGCTAAAAACTACCATTGAATCTCACACCAAGCCTTCTCCCAGAGTGCCAGGTAAATTGT CCCCGGCGAAACAGGCCCAACTGAGGAACTTCTTGGCCAAAAGGAAGACCCCACCAGTGAGGTCTACTGC TCCAGCCAGCCTGTCCCGGTCAGCCTTCCTATCTCAGAGGTACTATGAAGACTTAGATGAAGGCAGCTCC GCGTCATCCGTAGCTCAGCCTCTGGAGGGAGAAGATGCCCGGCCAACCTGCACATCTGTAGCTCAGCCTC TGGAGGGAGAAGATGCCCAGCCAATCTGCAAAGAGGAAGAGGCTGTGGTCCCAGTACCTCGGCATGCCCC TGTGGTCCGCACACCTTCTATCCAACCCAGTCTCCTGCCTCAGTCGATGCCCTTTGCAAAGCCTCACCTG ATCCATAGTTCTTCACCTGCCGTGATGAGCTCTGCAGTCTCTACGTCAGCCACCAAAGTTATTCCTCAGG GAGCAGACAGCACAATGCTGGCAACTAAAACAGTAAAGCACGGTGCACCAGGTCCTTCCCACACTGTTGC AGCTCCCCAGGCCGCAGCTGCTGCAGCCCTAAGACGGCAGATGGCCAGTCAGGCACCAGCTATGAGCACT CTGACCGAATCAACTCTGAAGACTGTCCCTCAGGTGGTGAATGTACAGGAACTGAGGAGTAATCCCTCGC CCCCATCTGCGGCCATGGGTTCTGCCGTGCAGCACTCAGCAGCCAAAACACCCCATGCTGTGTTAACCCC CGTGGCTAACAGCCAAGCCAAGCAGGGGTCTCTGATAAATTCTTTCAAGCCGTCAGGACCCACAGCAGCA TCCTGTCAGCTGTCCTCTGGGGATAAAGCTGTAGGTCAAGGGACAGCCAAGACAGAGTCCGCTGCGACCT CGACTCCATCTGCTGCAGGACAGCTCAACAAGCCTTTCTCATTCGCCTCCCCAGGGACCTTTACTTTTGG GACAATCACACCAACCCCATCTTCTAGTTTCACTGCCACACCAGGGGCAGGACCCCCTACTAAAGAGCCA ACCCAGCTTGAGGCATTCTCATTTGGCGGGGGAGGCAAACCTTTCTCTGAGGCCATTCCTGGGAACTCCC CTGCCACAGGAGCAACATCGGCACCCAGCACTTCTGTAACAGCTGCCTCTCTGGAAGACTCTGCTCCATC CAGCAGCAAACCTGCAGCTCCTCCTGAAACTACTGTGTCCTCTGCTTCTAGCAAATTGGAAACTCCACCG TCCAAGTTAGGAGAGCTTCTGTTCCCAAGCTCTCTAGCTGGAGAGACGCTGGGGAGCTTCTCGGGCCTGC GTGTTGGCCAAGCTGAGGATTCTACTAAGCCAGTTAGTAAGGCCTCCTCCACAAACCTAGCTGGAGCCCA GCCAGCTAAGCCCTCAGGGGTTAGCTTTCCTAATACTTCAGTGTTAGGGAAGCCAGTGGAGCCCGCTGTG ACCTCCTCTGTGAGCCCCGCCCCAGCAGCACCAGCCTCAGCTCTGAATGTGAGCACTAGCAGTTCCTCAG CAACTGTCTTTGGCAGTGTGCCCCTTACCAGTGCTGGACCTCCTGGGCTCATCAGTTTTGGTGGGGCTGC TTTGGCTAGTAGCAAAGCTAGTTTCTCATTTGGAAACCAGCAGACTAGTAGTAGTACAGCATCATCAGCT ACCCCAACATCGACCTCAGTTGCCCCTTCCCTTCCAGCATCTTTCCCTACTTTGTCATTTGGTGGCCTCC TGAGTTCACCATCAGCTTCTTCCCTGCCCGTGTCCTCTGGTAAGAGTACAGAGGAGGCAGCACCACCAGC TGTTCCTGACAAGTCAGACAGCAGTGAAGTTTCAGCAACAACACCCTCACTTCCAGTACAGCCACAGTCA ACCCAGGCATCTCTGCAAACTTCTGACCCTGTCAAAAAAGAACCTGTTCTTGTGCAGACCACAGACAGCA GCCCCAGCAGACCAGCATCTTCTGCCAGCTTTGTGGCATCCACAGAGTCCATGCCAGTAACCCTAGGGGC CCCTGACAGCAAGATAGAGGCAGTGTCTCCTGCGTCCACCTTTGCAGGGCCTGGACAGGCTGCTGTGGCA ACAGCTGTTCTCCCAGGAGCAGGCTCGGCAGCCACTGAAGCATCGGGTACCCCTACCACCTCCACTGTCT CCAGCAGTAGCCCAACTTCTGCCACAGAGACAGCTGTATTTGGGACTGCCACATCTGGTTCCTCAGTCTT TACACAGCCACCTGCTGCCAGTTCTAGCTCAGCGTTCAGCCAGCTCTCCAGTAACACAGCCACTGCGCCC TCTGCCACCCCTGTGTTTGGGCAAGTGGCAGCCAGCATAACCAGCACAGCCGCTGCCACACCACAGGCCA GCAGTTCAGGGTTTGGCAGCCCTGCATTTGGTGCTTCAGCTCCTGGAGTGTTCGGACAGACAGCATTTGG GCAGACCCCAGCCTTTGGGCAAGCAACAAGCAGCCCTGCCAGTGGCTTCTCCTTCAGCCAGCCTGGGTTC AGTTCCGTGCCTGCTTTCGGCCAGTCTGTCTCCTCTACCCCCGCATCCACCAGTGCAAATGTCTTTGGAG CCACCTCGAGTACCAGCAGTCCTGGTTCCTTCTCATTTGGACAGGCTTCTACCAATACAGGAGGGACGCT GTTTGGTCAAAACAACCCTCCTGCTTTTGGTCAGAGCCCTGGCTTTGGGCAGGGAAGCTCTGTGTTTGGT GGCACCTCGGCCACTACCTCTACAGCAGCACCTTCAGGGTTTAGCTTTTGTCAAGCCTCCGGTTTTGGGT CTAGTAATACTGGTTCTGTGTTTGGCCAGGCAGCCAACACTGGTGGGTCAGTCTTTGGCCAGTCATCCAC TTCCAGTGGTGGCGTGTTTGGGTCTGGAAATGCTACAAGAGGGGGCGGTTTCTTCAGTGGCCTTGGAGGA AAACCCAGCCAGGATGCTGCCAACAAAAACCCCTTCAGCTCAGCCGGTGGGGGCTTTGGATCTACAGCTG CCCCAAATACCTCTAATCTGTTTGGAAACAGTGGAGCTAAGACATTTGGAGGGTTTGGCAGCTCATCCTT CGGGGAACAGAAGCCTGCCGGCACATTCAGCTCTGGCGGTGGGAGCGTAGCATCCCAAGGTTTTGGATTT TCCACTCCAAATAAAACAGGTGGCTTCGGTGCTGCTCCAGTGTTTGGCAGCCCTCCTACTTTTGGGGGAT CCCCTGGGTTTGGAGGGGTGCCAGCATTCGGTTCAGCCCCAGCCTTTACAAGCCCTCTGGGCTCGACGGG AGGCAAAGTGTTCGGAGAGGGTACTGCGGCTGCCAGCGCAGGCGGATTCGGGTTCGGGAGCAGCGGCAAT ACTGCATCCTTCGGTACCCTCGCCAGTCAGAACGCACCCACCTTCGGGTCCTTGTCCCAGCAGACCTCAG GCTTTGGGACCCCGAGCAGTGGATTCGCTGGGTTTGGATCGGGCACAGGAGCCTTCACCTTTGGATCATC TAACTCGTCTGTCCAGGGCTTCGGTGGCTGGAGAAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG212020 representing BC039282
Red=Cloning site Green=Tags(s) MSSTPNTGMKPSFPPSASSVKVNLNEKFTAVASSAPVHSSTSTPSVLPFSSSPKPTASGPLSHPTPLPAS SSSMPLKSSVSPSPAAGRSTQTAPSSAPSTGQKSPRVNPPVPKSGSSQAKALQPPVTEKQRPQWKDSDPV LAGIGEEIAHFQKELEELKARTAKACLQVGTSEEMKMLRTESDDLHTFLFEIRETTESLHGDISTLKTTL LEGFAGVEEAREQHGRNHDSGYLHLLYKRPLDPKSEAQLQEIRRLHQYVKFAVQDVNDVLDLEWDRHLEQ KKRQRRLIVPERETLFNTLANNREIINQQRKRLNQLVDSLQQLRLYNHTAPWSLPSALSTQSNSHSFDSD LECLLKTTIESHTKPSPRVPGKLSPAKQAQLRNFLAKRKTPPVRSTAPASLSRSAFLSQRYYEDLDEGSS ASSVAQPLEGEDARPTCTSVAQPLEGEDAQPICKEEEAVVPVPRHAPVVRTPSIQPSLLPQSMPFAKPHL IHSSSPAVMSSAVSTSATKVIPQGADSTMLATKTVKHGAPGPSHTVAAPQAAAAAALRRQMASQAPAMST LTESTLKTVPQVVNVQELRSNPSPPSAAMGSAVQHSAAKTPHAVLTPVANSQAKQGSLINSFKPSGPTAA SCQLSSGDKAVGQGTAKTESAATSTPSAAGQLNKPFSFASPGTFTFGTITPTPSSSFTATPGAGPPTKEP TQLEAFSFGGGGKPFSEAIPGNSPATGATSAPSTSVTAASLEDSAPSSSKPAAPPETTVSSASSKLETPP SKLGELLFPSSLAGETLGSFSGLRVGQAEDSTKPVSKASSTNLAGAQPAKPSGVSFPNTSVLGKPVEPAV TSSVSPAPAAPASALNVSTSSSSATVFGSVPLTSAGPPGLISFGGAALASSKASFSFGNQQTSSSTASSA TPTSTSVAPSLPASFPTLSFGGLLSSPSASSLPVSSGKSTEEAAPPAVPDKSDSSEVSATTPSLPVQPQS TQASLQTSDPVKKEPVLVQTTDSSPSRPASSASFVASTESMPVTLGAPDSKIEAVSPASTFAGPGQAAVA TAVLPGAGSAATEASGTPTTSTVSSSSPTSATETAVFGTATSGSSVFTQPPAASSSSAFSQLSSNTATAP SATPVFGQVAASITSTAAATPQASSSGFGSPAFGASAPGVFGQTAFGQTPAFGQATSSPASGFSFSQPGF SSVPAFGQSVSSTPASTSANVFGATSSTSSPGSFSFGQASTNTGGTLFGQNNPPAFGQSPGFGQGSSVFG GTSATTSTAAPSGFSFCQASGFGSSNTGSVFGQAANTGGSVFGQSSTSSGGVFGSGNATRGGGFFSGLGG KPSQDAANKNPFSSAGGGFGSTAAPNTSNLFGNSGAKTFGGFGSSSFGEQKPAGTFSSGGGSVASQGFGF STPNKTGGFGAAPVFGSPPTFGGSPGFGGVPAFGSAPAFTSPLGSTGGKVFGEGTAAASAGGFGFGSSGN TASFGTLASQNAPTFGSLSQQTSGFGTPSSGFAGFGSGTGAFTFGSSNSSVQGFGGWRS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC039282 |
| ORF Size | 4587 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC039282.1 |
| RefSeq Size | 4962 bp |
| RefSeq ORF | 4589 bp |
| Locus ID | 227720 |
| Gene Summary | May serve as a docking site in the receptor-mediated import of substrates across the nuclear pore complex.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224588 | Nup214 (untagged) - Mouse nucleoporin 214 (cDNA clone MGC:37347 IMAGE:4976266), (10ug) |
CN¥ 19,190.00 |
|
| MR212020 | Nup214 (Myc-DDK-tagged) - Mouse nucleoporin 214 (cDNA clone MGC:37347 IMAGE:4976266) |
CN¥ 15,300.00 |
|
| MR212020L3 | Lenti ORF clone of Nup214 (Myc-DDK-tagged) - Mouse nucleoporin 214 (cDNA clone MGC:37347 IMAGE:4976266) |
CN¥ 17,200.00 |
|
| MR212020L4 | Lenti ORF clone of Nup214 (mGFP-tagged) - Mouse nucleoporin 214 (cDNA clone MGC:37347 IMAGE:4976266) |
CN¥ 17,200.00 |
