Nid2 (BC054746) Mouse Tagged ORF Clone

CAT#: MG211968

  • TrueORF®

Nid2 (tGFP-tagged) - Mouse nidogen 2 (cDNA clone MGC:65650 IMAGE:6405073)



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Mouse Protein / Lysate Request

CNY 11,880.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms Ly111, entactin-2, NID-2, nidogen-2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211968 representing BC054746
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTCGGGACCCGACAGCCGGGTGGCTAACACCGCCATCACCGCTATCGCTGCTGGTGATGCTGTTGC
TGCTGTCGCGAGTCGGGGCTCTGCGTCCCGACGAGCTCTTTCCTTACGGGGAGTCGTGGGGAGACCAGCT
GCTGCCGGAGGGCGACGACGAAAGCTCAGCAGCTGTGAAGCTGGCAATACCCCTGCGTTTCTACGATGCC
CAGTTCAGCAGCCTCTACGTGGGCACCAACGGCATCATCTCCACCCAGGACTTCCCCAGGGAGACTCAAT
ATGTAGATGATGATTTTCCTACTGACTTCCCAGCCATCGCCCCTTTCCTGGCTGACATCGACACTAGTCA
CAGCAGGGGCCGGATCCTGTACCGCGAGGACACCTCCGGAGCTGTGCTGAGCCTGGCTGCCCGCTACGTG
CGCACTGGCTTCCCGCTGTCGGGGTCCAGCTTCACCCCCACCCACGCCTTCCTGGCCACCTGGGAGCACG
TGGGCGCCTACGAGGAGGTCAGTCGCGGGGCTGCGCCGTCAGGAGAGCTGAACACTTTCCAGGCTGTTCT
GGCATCCGATGAATCTGATACCTACGCCCTCTTTCTCTACCCTGCCAATGGCCTTCAGTTCTTTGGAACC
CGCCCCAAAGAGTCCTACAATGTCCAGCTGCAGCTTCCCGCTAGGGTGGGCTTCTGCCGAGGGGAAGCGG
ACGACCTGAAGAGAGAAGCACTGTATTTCAGCCTGACTAACACCGAGCAGTCTGTGAAGAATCTCTACCA
GCTAAGCAACCTGGGGATTCCTGGAGTGTGGGCTTTTCACATCGGCAGCAGGTTTGCCCTGGACAATGTC
CGTCCAGCCACAGTTGGAGGTGACCCTTCCACAGCCCGTTCCTCAGCACTGGAGCACCCCTTCAGCCATG
CTGCAGCCCTGGAGAGCTACACCGAGGACAGTTTCCATTACTACGATGAGAATGAAGAGGATGTTGAGTA
CCCACCAGTTGAACCGGGGGAGGCCCCGGAAGGCCACAGCAGAATTGATGTGTCTTTCAATTCAAAGGCT
GATCCAGGCCTTGTGGACGTCGGCACCTCATCTCCAGGTTCCGACCGCGCTTCTCCTTGGCCATATCCAG
CACCTGGTAACTGGCCATCCTACCGGGAAACAGAATCGGCTTCTTTGGACCCTCAAACCAAACAGGGGCG
GCCGGTGGGAGAGGGAGAGGTTCTGGATTTCAGGGACCCAGCGGAGCTTTTGGACCAGATGGGTACCAGA
GCCCCAGCTCCTCCAGAGGCAGATGCAGCTCTCCTAACTCCAGTCAACGAAGACCTTGGGGGCAGAAACA
CCCAGTCCTATCCAGAGGCAGGGCCAGTGCCTTCAGAGCCAGATGTTCCTGTCCCTCCTCTGGAAGGAGA
AGTCCTTCCTCATTACCCCGAGTCTGGTCACGTTCCGCCCCTGAGAGGAGGGAAGTATGTGATAGGACTG
GAGGACCACGTGGGATCTAATGATCAAGTCTTCACGTATAATGGCGCCAACCTGGAAACCTGCGAACACA
GCCACGGCCGGTGCTCCCAGCATGCCTTCTGCACCGACTACACCACTGGCTTTTGCTGCCACTGCCAGTC
GAGGTTTTACGGAAACGGGAAGCACTGCCTGCCAGAAGGGGCACCTCACCGAGTGAATGGGAAAGTGAGT
GGTCGCCTCCGAGTGGGTCACATTCCTGTGCACTTCACTGACGTGGATCTACACGCTTACATCGTGGGCA
ATGATGGCAGAGCCTACACTGCCATCAGCCATGTCCCACAGCCGGCAGCCCAGGCCCTGCTCCCTGTCCT
GCCCATTGGAGGTCTGTTTGGCTGGCTCTTTGCTTTGGAGAAGCCAGGCTCTGAGAATGGCTTCAGCCTC
ACAGGTGCTACCTTTGTCCATGACGTGGAAGTCACTTTTCACCCTGGAGAGGAGAGGGTTCGTATCACTC
AAACTGCCGAGGGGCTTGACCCAGAAAACTATCTGAGCATTAAGACCAACATTGAAGGCCAGGTGCCCTT
CATCCCAGCGAATTTCACAGCCCACATTACTCCGTACAAAGAGTTTTATCATTACAGGGACTCAGTCGTG
ACGTCCTCCAGTTCCCGAAGTTTCTCTCTCACTTCTGGTTCTATTAACCAAACGTGGTCCTACCACATTG
ACCAGAACATCACTTACCAGGCGTGCAGGCACGCCCCCAGACACCTGGCCATCCCTGCCACCCAGCAGCT
GACTGTCGACCGGGCCTTTGCTTTGTACAGTGAGGATGAGGGCGTGCTGAGGTTTGCTGTGACCAATCAG
ATTGGCCCTGTTGAAGTGGACTCAGCCCCTGTTGGAGTGAATCCTTGCTACGACGGAAGCCACACCTGTG
ACACAACAGCAAGGTGCCACCCGGGAACAGGTGTAGACTACACCTGCGAGTGCACGCCTGGGTTCCAGGG
TGACGGACGGAGCTGTGTGGATGTCAATGAGTGTGCCACTGGCTTCCACCGCTGTGGCCCTAATTCTGTG
TGTGTCAACTTGGTGGGAAGCTACAGGTGTGAATGCCGCAGTGGCTATGAATTTGCAGATGACCAGCACA
CTTGTATCTTGATCGCCCCACCTCCCAACCCTTGCCTCGATGGCAGTCACACCTGTGCCCCTGAGGGGCA
AGCCAGGTGCATTCACCATGGAGGCAGCTCATTCAGTTGTGCCTGCCTGCCAGGCTTCATTGGCACTGGG
CATCAGTGTTCTGATGTTGATGAATGTGCGGAAAACCGATGTCATGAGGCAGCTATCTGCTACAATACCC
CCGGGTCCTTCTCCTGCCGTTGCCAGCCTGGGTATCGAGGGGACGGGTTTCACTGCACCTCTGACACGGT
TCCGGAAGATTCCATCTCAGGACTGAAGCCCTGTGAATACCAGCAGCGCTATGCTCAGACACAGCACGCC
TACCCTGGGTCACGGATCCACATCCCCCAGTGTGATGACCAGGGAAACTTCGTGCCACTGCAGTGCCACG
GCAGCACTGGCTTCTGCTGGTGTGTGGACCGAAATGGCCACGAAGTCCCTGGCACGCAGACTCCACCCGG
CTCCACCCCGCCCCACTGTGGACCACCTCCAGAGCCCACCCAGAGGCCTCGGACTGTCTGTGAGCGTTGG
CGGGAAAGCCTGCTGGAACACTACGGAGGCACACCCAGGGATGACCAGTATGTGCCCCAGTGTGATGACC
TGGGCCACTTCATCCCCCTGCAGTGCCATGGAAAGAGTGATTTCTGTTGGTGTGTGGACAAGGATGGCAG
AGAGCTACAGGGCACGCGCTCACAGCCAGGCACCAGGCCTGCATGCATACCCACCGTCGCTCCACCCGTG
GTCCGGCCCACACCCCGGCCCGATGTGACTCCTCCATCTGTGGGCACCTTCCTGCTCTATGCCCAGGGCC
AACAGATTGGCCACTTGCCCCTCAATGGCAGCAGGCTTCAGAAGGACGCAGCCCGGACCCTGCTGTCACT
GCATGGCTCCATAGTTGTGGGGATTGACTATGACTGCCGGGAGAGGATGGTCTACTGGACAGACGTTGCT
GGTCGGACCATCAGCCGTGCCAGCTTGGAGGCAGGAGCCGAACCCGAGACCATCATTACCTCAGGTTTGA
TAAGCCCGGAAGGACTTGCCATCGACCACTTCCGTAGAACAATGTACTGGACAGATAGTGGCCTGGATAA
GATAGAGCGGGCCGAACTGGATGGTTCCGAGCGGAAAGTCCTCTTCCACACAGATCTGGTGAATCCACGA
GCCATCACTGTGGATCCAATCCGAGGCAACTTGTACTGGACAGACTGGAATAGAGAAGCTCCTAAAATTG
AAACATCATCTTTAGATGGCGAAAACAGAAGGATTCTGATCAACAAAGATATTGGATTACCCAATGGATT
GACCTTTGACCCCTTCTCCAAACTGCTCTGCTGGGCAGATGCAGGAACCAAAAAACTGGAGTGTACACTA
CCCGATGGAACTGGACGACGTGTCATCCAAAACCACCTCAATTACCCCTTCAGCATCGTCAGCTATGCAG
ATCACTTCTACCACACGGACTGGCGGAGGGATGGTGTTATATCAGTGAATAAGGACAGTGGCCAGTTTAC
TGATGAGTTTCTCCCTGAGCAGCGGTCTCATCTCTATGGGATCACTGCAGTCTACCCGTACTGTCCAACA
GGAAGAAAA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211968 representing BC054746
Red=Cloning site Green=Tags(s)

MFRDPTAGWLTPPSPLSLLVMLLLLSRVGALRPDELFPYGESWGDQLLPEGDDESSAAVKLAIPLRFYDA
QFSSLYVGTNGIISTQDFPRETQYVDDDFPTDFPAIAPFLADIDTSHSRGRILYREDTSGAVLSLAARYV
RTGFPLSGSSFTPTHAFLATWEHVGAYEEVSRGAAPSGELNTFQAVLASDESDTYALFLYPANGLQFFGT
RPKESYNVQLQLPARVGFCRGEADDLKREALYFSLTNTEQSVKNLYQLSNLGIPGVWAFHIGSRFALDNV
RPATVGGDPSTARSSALEHPFSHAAALESYTEDSFHYYDENEEDVEYPPVEPGEAPEGHSRIDVSFNSKA
DPGLVDVGTSSPGSDRASPWPYPAPGNWPSYRETESASLDPQTKQGRPVGEGEVLDFRDPAELLDQMGTR
APAPPEADAALLTPVNEDLGGRNTQSYPEAGPVPSEPDVPVPPLEGEVLPHYPESGHVPPLRGGKYVIGL
EDHVGSNDQVFTYNGANLETCEHSHGRCSQHAFCTDYTTGFCCHCQSRFYGNGKHCLPEGAPHRVNGKVS
GRLRVGHIPVHFTDVDLHAYIVGNDGRAYTAISHVPQPAAQALLPVLPIGGLFGWLFALEKPGSENGFSL
TGATFVHDVEVTFHPGEERVRITQTAEGLDPENYLSIKTNIEGQVPFIPANFTAHITPYKEFYHYRDSVV
TSSSSRSFSLTSGSINQTWSYHIDQNITYQACRHAPRHLAIPATQQLTVDRAFALYSEDEGVLRFAVTNQ
IGPVEVDSAPVGVNPCYDGSHTCDTTARCHPGTGVDYTCECTPGFQGDGRSCVDVNECATGFHRCGPNSV
CVNLVGSYRCECRSGYEFADDQHTCILIAPPPNPCLDGSHTCAPEGQARCIHHGGSSFSCACLPGFIGTG
HQCSDVDECAENRCHEAAICYNTPGSFSCRCQPGYRGDGFHCTSDTVPEDSISGLKPCEYQQRYAQTQHA
YPGSRIHIPQCDDQGNFVPLQCHGSTGFCWCVDRNGHEVPGTQTPPGSTPPHCGPPPEPTQRPRTVCERW
RESLLEHYGGTPRDDQYVPQCDDLGHFIPLQCHGKSDFCWCVDKDGRELQGTRSQPGTRPACIPTVAPPV
VRPTPRPDVTPPSVGTFLLYAQGQQIGHLPLNGSRLQKDAARTLLSLHGSIVVGIDYDCRERMVYWTDVA
GRTISRASLEAGAEPETIITSGLISPEGLAIDHFRRTMYWTDSGLDKIERAELDGSERKVLFHTDLVNPR
AITVDPIRGNLYWTDWNREAPKIETSSLDGENRRILINKDIGLPNGLTFDPFSKLLCWADAGTKKLECTL
PDGTGRRVIQNHLNYPFSIVSYADHFYHTDWRRDGVISVNKDSGQFTDEFLPEQRSHLYGITAVYPYCPT
GRK

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC054746
ORF Size 4211 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC054746, AAH54746
RefSeq Size 4885 bp
RefSeq ORF 4211 bp
Locus ID 18074
Gene Summary Cell adhesion glycoprotein. Might be involved in osteoblast differentiation. It probably has a role in cell-extracellular matrix interactions.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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