Mapk8ip3 (BC060603) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Jip3, JSAP1, JSAP1a, JSAP1b, JSAP1c, JSAP1d, Syd2, mKIAA1066
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211916 representing BC060603 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGGAGATCCAGATGGACGAGGGAGGAGGAGTGGTGGTGTACCAAGACGACTACTGCTCGGGCTCGG TCATGTCGGAGCGTGTGTCGGGCCTGGCGGGCTCCATCTACCGCGAGTTCGAGCGCCTCATTCACTGCTA TGACGAGGAGGTGGTCAAGGAGCTCATGCCGCTGGTGGTGAACGTGCTGGAGAACCTTGACTCGGTGCTG AGCGAGAACCAGGAGCACGAGGTGGAGCTGGAGCTCCTACGCGAGGACAACGAGCAGCTGCTCACGCAAT ACGAGCGCGAGAAGGCGCTGCGCAAACAGGCCGAGGAGAAATTCATCGAATTTGAAGATGCCTTGGAACA AGAGAAGAAAGAACTCCAGATCCAGGTAGAACATTATGAGTTTCAGACACGCCAGCTGGAGCTAAAGGCC AAAAACTATGCAGATCAGATTTCCCGACTGGAGGAACGAGAATCGGAGATGAAGAAGGAATACAATGCCC TGCACCAGCGGCACACAGAGATGATCCAGACCTATGTGGAACACATTGAAAGATCCAAGATGCAGCAAGT TGGGGGTAGCGGCCAAACAGAAAGCAGCCTGCCCGGGCGGAGTCCTCGCCAGTCGTGGAGGAAAAGCAGG AAGGAGCGTCCCACCTCTCTGAATGTCTTCCCCCTGGCTGATGGCATGTGTCCAAATGATGAGATGTCTG AGTCAGGCCAGTCCTCAGCAGCTGCAACACCCAGTACCACAGGTACCAAGTCCAACACACCCACGTCCTC CGTGCCCTCAGCAGCAGTCACGCCACTCAACGAGAGCCTACAGCCCCTGGGGGACTATGTCAGTGTCACA AAGAACAACAAGCAGGCCCGAGAGAAGCGCAATAGCCGTAACATGGAGGTCCAGGTCACCCAAGAGATGC GGAACGTCAGTATCGGCATGGGCAGCAGTGACGAGTGGTCCGATGTTCAGGACATTATCGACTCCACCCC AGAGCTGGATGTGTGTCCTGAAACCCGTCTGGAGCGCACAGGAAGCAGCCCAACCCAGGGAATTGTAAAC AAAGCTTTTGGAATCAACACTGACTCCTTGTATCACGAACTCTCCACGGCGGGATCTGAGGTCATCGGGG ATGTGGACGAGGGAGCTGATCTCCTAGGGGAGTTTTCAGTGCGCGATGATTTTTTTGGAATGGGCAAAGA AGTGGGGAACCTGCTGCTGGAGAACTCACAGCTTCTAGAGACAAAAAATGCTTTAAATGTAGTGAAGAAT GACCTCATTGCTAAGGTTGACCAACTGTCAGGAGAACAGGAGGTCCTGAAGGGTGAGCTGGAAGCAGCCA AGCAAGCCAAAGTCAAGCTGGAGAACCGAATCAAAGAGCTTGAAGAAGAACTGAAGAGAGTCAAGTCAGA GGCAGTAACTGCCCGCCGTGAGCCCAGAGAAGAGGTGGAGGATGTAAGCAGCTATCTCTGTACAGAATTG GACAAAATCCCCATGGCCCAGCGCCGACGCTTCACACGGGTGGAGATGGCCCGAGTGCTCATGGAACGCA ACCAGTACAAGGAACGCCTCATGGAGCTGCAGGAGGCTGTGAGGTGGACTGAAATGATCAGAGCATCAAG GGAACACCCATCTGTCCAGGAGAAGAAGAAGTCCACCATCTGGCAGTTCTTTAGTCGCCTCTTCAGCTCC TCATCTAGCCCCCCTCCGGCCAAACGATCCTACCCATCTGTGAACATTCACTACAAGTCACCCACTGCAG CTGGCTTTAGCCAGCGTCGCAGCCATGCTTTGTGCCAGATCTCAGCCGGCAGCAGGCCCCTGGAGTTCTT CCCTGATGATGACTGCACCTCTTCTGCCCGGCGGGAGCAGAAGCGGGAGCAGTACCGCCAGGTTCGTGAA CACGTGCGCAATGATGACGGGAGGCTGCAGGCCTGTGGGTGGAGCCTGCCTGCCAAGTACAAGCAGCTGA GCCCCAATGGAGGCCAGGAAGACACCCGGATGAAAAATGTGCCTGTCCCTGTGTACTGCCGCCCTCTGGT GGAGAAGGACCCTTCGACAAAGCTGTGGTGTGCTGCTGGTGTCAACCTGAGTGGGTGGAAGCCACATGAA GAGGACTCTAGCAATGGACCCAAGCCTGTACCAGGTCGAGACCCTCTGACCTGTGACCGGGAAGGAGAAG GCGAACCCAAGAGCACACACCCATCACCTGAGAAGAAGAAGGCAAAGGAAACCCCTGAGGCAGATGCTAC CTCCAGTCGGGTATGGATCCTCACCAGCACCCTGACAACCAGCAAGGTGGTGATCATTGATGCCAACCAG CCAGGCACAATTGTGGATCAGTTCACAGTCTGCAATGCCCACGTCCTGTGTATCTCCAGCATTCCTGCGG CCAGTGACAGTGACTATCCCCCTGGGGAGATGTTCCTAGACAGTGATGTGAACCCTGAAGATTCAGGTGC TGATGGTGTGCTGGCTGGCATCACCCTGGTGGGGTGTGCTACCCGCTGCAATGTTCCACGTAGCAACTGT TCCTCACGAGGAGACACCCCAGTACTGGACAAGGGGCAGGGGGATGTGGCGACCACTGCCAATGGGAAGG TCAACCCGTCCCAATCCACAGAAGAAGCCACAGAAGCCACGGAGGTGCCAGACCCTGGTCCCAGCGAGTC AGAAGCAACGACAGTCCGGCCCGGGCCTCTCACAGAGCATGTCTTTACTGACCCAGCACCCACCCCATCC TCCAGCACCCAGCCTGCCAGTGAGAATGGGTCAGAGTCCAATGGCACCATTGTACAGCCTCAGGTGGAGC CCAGTGGGGAACTCTCAACAACAACCAGTAGCGCTGCACCCACTATGTGGCTAGGAGCCCAGAATGGCTG GCTCTATGTGCATTCAGCGGTAGCCAACTGGAAGAAGTGTCTGCACTCCATCAAGCTAAAAGACTCTGTG CTGAGCCTGGTGCATGTCAAAGGCCGAGTGCTGGTAGCTCTTGCAGATGGGACCCTGGCTATCTTCCATC GTGGAGAGGATGGCCAGTGGGACCTGAGCAACTACCACCTAATGGACCTGGGCCACCCACACCACTCCAT CCGCTGCATGGCTGTTGTGAATGACCGAGTTTGGTGTGGCTACAAGAACAAGGTGCATGTTATCCAGCCC AAGACAATGCAGATTGAGAAATCATTTGATGCCCACCCAAGGCGGGAAAGCCAGGTACGTCAGCTGGCCT GGATCGGTGATGGAGTGTGGGTCTCTATTCGCTTGGATTCTACCCTTCGGCTCTACCATGCTCACACCCA CCAGCACCTGCAGGATGTGGACATTGAGCCCTATGTTAGCAAGATGCTAGGAACCGGCAAGCTGGGCTTC TCCTTCGTGCGCATCACAGCCTTACTCATTGCAGGCAACCGTCTGTGGGTGGGCACTGGCAATGGGGTTG TCATCTCCATCCCCTTGACTGAGACTGTGGTCCTGCATCGAGGCCAGCTCCTAGGGCTCCGAGCCAACAA GACATCCCCAACATCTGGGGAGGGGACCCGCCCAGGGGGCATCATCCATGTGTATGGGGACGACAGCAGT GACAAGGCCGCCAGTAGTTTCATCCCCTACTGCTCCATGGCACAGGCTCAGCTTTGCTTCCATGGGCACC GTGATGCTGTCAAATTCTTTGTCTCTGTGCCAGGAAATGTGCTGGCCACTCTCAATGGCAGTGTGCTAGA CAGCCCATCAGAGGGCCCTGGGCCTGCTGCACCCGCTGCAGATGCTGAGGGCCAGAAGTTGAAGAATGCA CTGGTGCTGAGTGGTGGTGAAGGTTACATTGACTTCCGTATCGGAGACGGAGAGGATGATGAAACTGAGG AATGTGCCGGGGACGTGAACCAGACAAAGCCCTCGTTGTCCAAGGCTGAGCGCAGCCACATCATCGTGTG GCAGGTGTCCTACACCCCTGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211916 representing BC060603 Red=Cloning site Green=Tags(s) MMEIQMDEGGGVVVYQDDYCSGSVMSERVSGLAGSIYREFERLIHCYDEEVVKELMPLVVNVLENLDSVL SENQEHEVELELLREDNEQLLTQYEREKALRKQAEEKFIEFEDALEQEKKELQIQVEHYEFQTRQLELKA KNYADQISRLEERESEMKKEYNALHQRHTEMIQTYVEHIERSKMQQVGGSGQTESSLPGRSPRQSWRKSR KERPTSLNVFPLADGMCPNDEMSESGQSSAAATPSTTGTKSNTPTSSVPSAAVTPLNESLQPLGDYVSVT KNNKQAREKRNSRNMEVQVTQEMRNVSIGMGSSDEWSDVQDIIDSTPELDVCPETRLERTGSSPTQGIVN KAFGINTDSLYHELSTAGSEVIGDVDEGADLLGEFSVRDDFFGMGKEVGNLLLENSQLLETKNALNVVKN DLIAKVDQLSGEQEVLKGELEAAKQAKVKLENRIKELEEELKRVKSEAVTARREPREEVEDVSSYLCTEL DKIPMAQRRRFTRVEMARVLMERNQYKERLMELQEAVRWTEMIRASREHPSVQEKKKSTIWQFFSRLFSS SSSPPPAKRSYPSVNIHYKSPTAAGFSQRRSHALCQISAGSRPLEFFPDDDCTSSARREQKREQYRQVRE HVRNDDGRLQACGWSLPAKYKQLSPNGGQEDTRMKNVPVPVYCRPLVEKDPSTKLWCAAGVNLSGWKPHE EDSSNGPKPVPGRDPLTCDREGEGEPKSTHPSPEKKKAKETPEADATSSRVWILTSTLTTSKVVIIDANQ PGTIVDQFTVCNAHVLCISSIPAASDSDYPPGEMFLDSDVNPEDSGADGVLAGITLVGCATRCNVPRSNC SSRGDTPVLDKGQGDVATTANGKVNPSQSTEEATEATEVPDPGPSESEATTVRPGPLTEHVFTDPAPTPS SSTQPASENGSESNGTIVQPQVEPSGELSTTTSSAAPTMWLGAQNGWLYVHSAVANWKKCLHSIKLKDSV LSLVHVKGRVLVALADGTLAIFHRGEDGQWDLSNYHLMDLGHPHHSIRCMAVVNDRVWCGYKNKVHVIQP KTMQIEKSFDAHPRRESQVRQLAWIGDGVWVSIRLDSTLRLYHAHTHQHLQDVDIEPYVSKMLGTGKLGF SFVRITALLIAGNRLWVGTGNGVVISIPLTETVVLHRGQLLGLRANKTSPTSGEGTRPGGIIHVYGDDSS DKAASSFIPYCSMAQAQLCFHGHRDAVKFFVSVPGNVLATLNGSVLDSPSEGPGPAAPAADAEGQKLKNA LVLSGGEGYIDFRIGDGEDDETEECAGDVNQTKPSLSKAERSHIIVWQVSYTPE TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC060603
ORF Size	3944 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC060603, AAH60603
RefSeq Size	5440 bp
RefSeq ORF	3944 bp
Locus ID	30957
Gene Summary	The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module. May function as a regulator of vesicle transport, through interactions with the JNK-signaling components and motor proteins (PubMed:10523642, PubMed:10629060). Promotes neuronal axon elongation in a kinesin- and JNK-dependent manner (PubMed:23576431, PubMed:25944905, PubMed:28259553). Activates cofilin at axon tips via local activation of JNK, thereby regulating filopodial dynamics and enhancing axon elongation (PubMed:23576431, PubMed:25944905, PubMed:28259553). Its binding to kinesin heavy chains (KHC), promotes kinesin-1 motility along microtubules and is essential for axon elongation and regeneration (PubMed:23576431, PubMed:25944905, PubMed:28259553). Regulates cortical neuronal migration by mediating NTRK2/TRKB anterograde axonal transport during brain development (PubMed:23576431, PubMed:25944905, PubMed:28259553). Acts as an adapter that bridges the interaction between NTRK2/TRKB and KLC1 and drives NTRK2/TRKB axonal but not dendritic anterograde transport, which is essential for subsequent BDNF-triggered signaling and filopodia formation (PubMed:23576431, PubMed:25944905, PubMed:28259553).[UniProtKB/Swiss-Prot Function]

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