Rbm16 (BC075621) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	KIAA1116, mKIAA1116, A630086M08Rik
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211875 representing BC075621 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCCGTGAAGACCTTCAACAGCGAGTTGTATTCACTGAATGACTATAAGCCACCCATTTCTAAAG CAAAAATGACCCAAATTACAAAGGCAGCCATCAAAGCTATTAAGTTTTATAAACATGTGGTACAAAGTGT TGAGAAATTCATTCAGAAATGTAAACCAGAATACAAGGTACCTGGACTATATGTTATTGACTCCATTGTG CGACAATCCCGGCATCAGTTTGGTCAAGAAAAGGATGTGTTTGCACCCAGATTTAGTAATAACATCATTA GCACTTTTCAGAATTTATATCGTTGCCCTGGGGATGACAAGAGTAAAATAGTGAGAGTATTAAACTTATG GCAGAAGAATAATGTTTTTAAGAGTGAGATTATCCAGCCTCTTCTGGATATGGCGGCAGGGATTCCTCCC CCAGTTGTCACGCCTGTTTTGGCCAGCACCACTGCAGCTATGAGCAACACTCCAGGAACACCCGTGACAC CTGTTACTCCAGCCAATGTGGTCCAAGGCTTACCTGATCCATGGGTATCTCAGATAGCAAACACAGACAC ACTGGCAGCTGTTGCTCAGATCCTACAAAGTCCTCAAGGTCAACAGCTTCAGCAGCTAATACAAACCTTA CAGATACAGCAGCAGAAGCCTCAGCCTTCTATCCTGCAGGCCTTGGATGCTGGTCTTGTGGTTCAGTTAC AGGCACTCACTGCACAGCTCACAGCTGCAGCTGCTGCAGCCAACACACTTACGCCCTTGGATCAGGGAGT CTCTTTCAACAAGAAGTTGATGGATAGATTTGATTTTGGGGAAGATTCTGAGCATAGTGAAGAATCCAAA AAGGAAATGCCTACTCCTCAACTCTCTCACGTTTCAGAGTCTGTGAACAACTCCATCTTCCATCAGATAG CAGAACAGCTGCAGCAGCAGAACTTGGAGCAGTTGCGACAGCAGCTCCTGGAGCAGCAGCAGCCTCAGAA GGTAACTCCTCAAGATAGCCAGGAGGGGACATTTGGGTCTGAGCACTCAGCTTCTCCCTCACAAGGGAGT AGCCAACAGCATTTTCTGGAACCTGAAGCAAATTTAGATGATTCCATAGACATTCAGCAACAGGATATGG ATATTGATGAAGGGCAAGACGTTGTTGAAGAGGAGATCTTTGAACCAGAAGCTAAGAAAGTAGCTGTTCG CTCAAGATCAAGAACTCATTCACGATCTCGATCAAGGTCACCAAGAAAACGAAGGTCCAGGTCACGCTCT GGGTCCCGAAAGCGAAAGCACAGGAAGCGGTCTCGGTCCCACTCGAGAGAGAAAAAGAGAAAAGCCTCAC GCTCCTACTCCAGTGAGCGCAGAGCCAGGGAAAGGGAGAAAGAGCGCCAGAAAAAGGGCTTGCCTCCTGT CAGGTCAAAAACACTAAGTGTATGCAGCACTACTCTCTGGGTTGGCCAAGTGGACAAGAAGGCTACACAG CAAGATTTAACCAACCTTTTTGAGGAATTTGGACAAATTGAATCCATTAATATGATTCCACCCAGGGGCT GTGCATATGTCTGCATGGTTCATCGACAAGATTCATTTCGGGCTCTTCAGAAACTGAGTTCAGGATCCTA TAAAATTGGTTCCAAGGTCATTAAGATTGCCTGGGCTTTGAACAAAGGTGTAAAGACAGAATACAAACAG TTCTGGGATGTGGATCTTGGCGTCACATATATACCCTGGGAAAAAGTGAAAGTAGATGACTTGGATGGCT TTGCAGAGGGCGGCATGATTGATCAGGAGACTGTAAATGCTGAATGGGAAACTGTGAAAGCCTCAGAACC TGTTAAAGAGCCAGTGCAGACTGCACAGAGTCCAGCTCCAGTTGAGAAGGAGTCAGTGGTCACCACACAG GCAGAGGTTTTCCCTCCTCCTGTCGCCATGCTGCAGATTCCTGTAGCACCAGCTGTTCCTGCAGTTAGCT TAGTTCCTCCAGCGTTTCCTGTGTCGATGCCTGTCCCCCCTCCTGGATTCAACCCGATCCCTCCACCTCC TTTTCTACGAGCAAGTTTTAACCCTTCACAACCACCTCCTGGTTTTATGCCACCTCCAGTTCCCCCACCT GTGGTACCTCCCCCTGCAATCCCACCGGTAGTACCAACATCTTTAGTGCAACCACCATTGTCCATGACCC CTGAAGCTGTGAAAGATGTTGGATTTGGCAGCCTGGTTTTACCAAGTGGTTCTGTTGCTGGCAGTCTTGC TCCTTCAACTCTACCAGCTGGAAATGTTTTTAATCCTCCCAGTAAAGCAGAGCCTGAAGAAAAAGTACCT CACCTTATAGAGCACCAGATTCCTTCTGGTGAGAACACAAGACCAGTGATTCCAAGTGATATTCCAAGTA GTGCTGCAATGTTAGCACAGCCGCCTGGGGCCTCAAGCACCTCTGGGATCCTGTGCGTGCAGAGACCAAA TGTATCAAGTAATTCTGAAATTCTGGGGGTTCGTCCAGCTAATGTTTCCAACAGTGCTGCAATTATGGGA GCCCAGCCACCAAATATTCTAAATAACTCTGGAATTTTGGCCATACAGCCACCAAATGTGTCCAGTGGCT CTGGACTTCTTGGGGTACTGCCTCCAAACTTGCCTAACAACTCTGGACTTGTAGGACTACAGCCACCAAA TGTTACAAGTCCTGCTGGACTTTTGGGAACACAGCCACCAATTGGACCTCAAAACTTACCACCTTTAGCC ATCCCTGCTCAAAGGATGCCCGCGTTGCCAATGTTAGACATTCGCCCAGGACTGATAGCACAGGCTCCCG GGCCACGATTCCCTTTACTACAGCCTGGAATTCCACCACAACGCGGTATCCCTCCCCCATCGGTACTTGA TGCAGCTCTTCATCCTCCACCCCGCGGACCTTTTCCTCCAGGAGATCTTTTCAGTCAGCCAGAAAGACCT TTTCTGGCCCCTGGAAGACCAAGTATAGACAATGTTCCCAACCCAGATAAAAGAATACCACTTGGGAATG ACAATATTCAGCAGGAAGGGGATAGAGATTACCGCTTTCCTCCTATAGAAACCAGGGAGGGCATTACCAG ACCTCCTCAGGTGGATGTTAGGGATGTGGTTGGGCGACGTCTAGATCCCAGAGAAGGCCCTGGAAGGCCT CCATTAGATGCTAGGGATCATTTTGGAAGACCTCCTGTGGACATGAGGGAGAATCTTGTGAGGCCAAGTC TAGACCACCTTGGTCGAAGAGACCACTTTGGCTTTCCCCCAGAGAAGCCTTGGGGGCCTAGAGATTTTGA TGAGAGAGAGCATCGAGTTCTGCCTGTCTTTGGTGGTCCAAAAGGCTTACATGAAGAAAGAGGTAGATTT CGGGCTGGAAATTACCGATTTGATCCTAGAAGTGGTCCTTGGAACAGAGGATTTGGTCAAGAAGTTCACA GAGATTTTGATGACCGCAGAAGACCCTGGGAGAGGCAGAGGGATAGGGATGACAGAGATTTTGATTTCTG CAGAGAAATTAATGGAAATCGTCTTGGACGAGATAGAATTCAAAACACTTGGGTTCCCCCTCCTCATGCT CGGGTTTTTGATTATTTCGAAGGGGCCACTTCTCAACGGAAAGGTGATAATGTGCCTCAAGTTAATGGTG AAAATACAGAGAGACATGCTCAGCCACCGCCTTTACCAGTACAGAAGGACCCTGAACTCTATGAGAAACT GGCGTCTTCAGGTGACGTAGACAAGGAGGAGAGCGGCACAGTTGCTGGTGTAGAGAGTGAAGCGGTGGTA GAAAGCACAGAGACTGAGGGGACA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211875 representing BC075621 Red=Cloning site Green=Tags(s) MEAVKTFNSELYSLNDYKPPISKAKMTQITKAAIKAIKFYKHVVQSVEKFIQKCKPEYKVPGLYVIDSIV RQSRHQFGQEKDVFAPRFSNNIISTFQNLYRCPGDDKSKIVRVLNLWQKNNVFKSEIIQPLLDMAAGIPP PVVTPVLASTTAAMSNTPGTPVTPVTPANVVQGLPDPWVSQIANTDTLAAVAQILQSPQGQQLQQLIQTL QIQQQKPQPSILQALDAGLVVQLQALTAQLTAAAAAANTLTPLDQGVSFNKKLMDRFDFGEDSEHSEESK KEMPTPQLSHVSESVNNSIFHQIAEQLQQQNLEQLRQQLLEQQQPQKVTPQDSQEGTFGSEHSASPSQGS SQQHFLEPEANLDDSIDIQQQDMDIDEGQDVVEEEIFEPEAKKVAVRSRSRTHSRSRSRSPRKRRSRSRS GSRKRKHRKRSRSHSREKKRKASRSYSSERRAREREKERQKKGLPPVRSKTLSVCSTTLWVGQVDKKATQ QDLTNLFEEFGQIESINMIPPRGCAYVCMVHRQDSFRALQKLSSGSYKIGSKVIKIAWALNKGVKTEYKQ FWDVDLGVTYIPWEKVKVDDLDGFAEGGMIDQETVNAEWETVKASEPVKEPVQTAQSPAPVEKESVVTTQ AEVFPPPVAMLQIPVAPAVPAVSLVPPAFPVSMPVPPPGFNPIPPPPFLRASFNPSQPPPGFMPPPVPPP VVPPPAIPPVVPTSLVQPPLSMTPEAVKDVGFGSLVLPSGSVAGSLAPSTLPAGNVFNPPSKAEPEEKVP HLIEHQIPSGENTRPVIPSDIPSSAAMLAQPPGASSTSGILCVQRPNVSSNSEILGVRPANVSNSAAIMG AQPPNILNNSGILAIQPPNVSSGSGLLGVLPPNLPNNSGLVGLQPPNVTSPAGLLGTQPPIGPQNLPPLA IPAQRMPALPMLDIRPGLIAQAPGPRFPLLQPGIPPQRGIPPPSVLDAALHPPPRGPFPPGDLFSQPERP FLAPGRPSIDNVPNPDKRIPLGNDNIQQEGDRDYRFPPIETREGITRPPQVDVRDVVGRRLDPREGPGRP PLDARDHFGRPPVDMRENLVRPSLDHLGRRDHFGFPPEKPWGPRDFDEREHRVLPVFGGPKGLHEERGRF RAGNYRFDPRSGPWNRGFGQEVHRDFDDRRRPWERQRDRDDRDFDFCREINGNRLGRDRIQNTWVPPPHA RVFDYFEGATSQRKGDNVPQVNGENTERHAQPPPLPVQKDPELYEKLASSGDVDKEESGTVAGVESEAVV ESTETEGT TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC075621
ORF Size	3804 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC075621, AAH75621
RefSeq Size	4885 bp
RefSeq ORF	3806 bp
Locus ID	106583
Gene Summary	Anti-terminator protein required to prevent early mRNA termination during transcription. Together with SCAF4, acts by suppressing the use of early, alternative poly(A) sites, thereby preventing the accumulation of non-functional truncated proteins. Mechanistically, associates with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit (POLR2A), and subsequently binds nascent RNA upstream of early polyadenylation sites to prevent premature mRNA transcript cleavage and polyadenylation. Independently of SCAF4, also acts as a positive regulator of transcript elongation.[UniProtKB/Swiss-Prot Function]

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