Inpp5d (NM_010566) Mouse Tagged ORF Clone
CAT#: MG211781
- TrueORF®
Inpp5d (tGFP-tagged) - Mouse inositol polyphosphate-5-phosphatase D (Inpp5d)
"NM_010566" in other vectors (5)
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CNY 13,110.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | p150Ship; s-SHIP; SHIP; SHIP-1; SHIP1; SIP-145 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG211781 representing NM_010566
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCTGCCATGGTCCCTGGGTGGAACCATGGCAACATCACCCGCTCCAAGGCAGAGGAGCTACTTTCCA GAGCCGGCAAGGACGGGAGCTTCCTTGTGCGTGCCAGCGAGTCCATCCCCCGGGCCTACGCACTCTGCGT GCTGTTCCGGAATTGTGTTTACACTTACAGGATTCTGCCCAATGAGGACGATAAATTCACTGTTCAGGCA TCCGAAGGTGTCCCCATGAGGTTCTTCACGAAGCTGGACCAGCTCATCGACTTTTACAAGAAGGAAAACA TGGGGCTGGTGACCCACCTGCAGTACCCCGTGCCCCTGGAGGAGGAGGATGCTATTGATGAGGCTGAGGA GGACACTGAAAGTGTCATGTCACCACCTGAGCTGCCTCCCAGAAACATTCCTATGTCTGCCGGGCCCAGC GAGGCCAAGGACCTTCCTCTTGCAACAGAGAACCCCCGAGCCCCTGAGGTCACCCGGCTGAGTCTCTCCG AGACACTGTTTCAGCGTCTACAGAGCATGGATACCAGTGGGCTTCCCGAGGAGCACCTGAAAGCCATCCA GGATTATCTGAGCACTCAGCTCCTCCTGGATTCCGACTTTTTGAAGACGGGCTCCAGCAACCTCCCTCAC CTGAAGAAGCTGATGTCACTGCTCTGCAAGGAGCTCCATGGGGAAGTCATCAGGACTCTGCCATCCCTGG AGTCTCTGCAGAGGTTGTTTGACCAACAGCTCTCCCCAGGCCTTCGCCCACGACCTCAGGTGCCCGGAGA GGCCAGTCCCATCACCATGGTTGCCAAACTCAGCCAATTGACAAGTCTGCTGTCTTCCATTGAAGATAAG GTCAAGTCCTTGCTGCACGAGGGCTCAGAATCTACCAACAGGCGTTCCCTTATCCCTCCGGTCACCTTTG AGGTGAAGTCAGAGTCCCTGGGCATTCCTCAGAAAATGCATCTCAAAGTGGACGTTGAGTCTGGGAAACT GATCGTTAAGAAGTCCAAGGATGGTTCTGAGGACAAGTTCTACAGCCACAAAAAAATCCTGCAGCTCATT AAGTCCCAGAAGTTTCTAAACAAGTTGGTGATTTTGGTGGAGACGGAGAAGGAGAAAATCCTGAGGAAGG AATATGTTTTTGCTGACTCTAAGAAAAGAGAAGGCTTCTGTCAACTCCTGCAGCAGATGAAGAACAAGCA TTCGGAGCAGCCAGAGCCTGACATGATCACCATCTTCATTGGCACTTGGAACATGGGTAATGCACCCCCT CCCAAGAAGATCACGTCCTGGTTTCTCTCCAAGGGGCAGGGAAAGACACGGGACGACTCTGCTGACTACA TCCCCCATGACATCTATGTGATTGGCACCCAGGAGGATCCCCTTGGAGAGAAGGAGTGGCTGGAGCTACT CAGGCACTCCCTGCAAGAAGTCACCAGCATGACATTTAAAACAGTTGCCATCCACACCCTCTGGAACATT CGCATAGTGGTGCTTGCCAAGCCAGAGCATGAGAATCGGATCAGCCATATCTGCACTGACAACGTGAAGA CAGGCATCGCCAACACCCTGGGAAACAAGGGAGCAGTGGGAGTGTCCTTCATGTTCAATGGAACCTCCTT GGGGTTCGTCAACAGCCACTTGACTTCTGGAAGCGAAAAAAAGCTCAGGAGAAATCAAAACTATATGAAC ATCCTGCGGTTCCTGGCCCTGGGAGACAAGAAGCTAAGCCCATTTAACATCACCCACCGCTTCACCCACC TCTTCTGGCTTGGGGATCTCAACTACCGCGTGGAGCTGCCCACTTGGGAGGCAGAGGCCATCATCCAGAA GATCAAGCAACAGCAGTATTCAGACCTTCTGGCCCACGACCAACTGCTCCTGGAGAGGAAGGACCAGAAG GTCTTCCTGCACTTTGAGGAGGAAGAGATCACCTTCGCCCCCACCTATCGATTTGAAAGACTGACCCGGG ACAAGTATGCATACACGAAGCAGAAAGCAACAGGGATGAAGTACAACTTGCCGTCCTGGTGCGACCGAGT CCTCTGGAAGTCTTACCCGCTGGTGCATGTGGTCTGTCAGTCCTATGGCAGTACCAGTGACATCATGACG AGTGACCACAGCCCTGTCTTTGCCACGTTTGAAGCGGGAGTCACATCTCAATTCGTCTCCAAGAATGGTC CTGGCACTGTAGATAGCCAAGGGCAGATCGAGTTTCTTGCATGCTACGCCACACTGAAGACCAAGTCCCA GACTAAGTTCTACTTGGAGTTCCACTCAAGCTGCTTAGAGAGTTTTGTCAAGAGTCAGGAAGGAGAGAAT GAAGAGGGAAGTGAAGGAGAGCTGGTGGTACGGTTTGGAGAGACTCTTCCCAAGCTAAAGCCCATTATCT CTGACCCCGAGTACTTACTGGACCAGCATATCCTGATCAGCATTAAATCCTCTGACAGTGACGAGTCCTA TGGTGAAGGCTGCATTGCCCTTCGCTTGGAGACCACAGAGGCTCAGCATCCTATCTACACGCCTCTCACC CACCATGGGGAGATGACTGGCCACTTCAGGGGAGAGATTAAGCTGCAGACCTCCCAGGGCAAGATGAGGG AGAAGCTCTATGACTTTGTGAAGACAGAGCGGGATGAATCCAGTGGAATGAAATGCTTGAAGAACCTCAC CAGCCATGACCCTATGAGGCAATGGGAGCCTTCTGGCAGGGTCCCTGCATGTGGTGTCTCCAGCCTCAAT GAGATGATCAATCCAAACTACATTGGTATGGGGCCTTTTGGACAGCCCCTGCATGGGAAATCAACCCTGT CCCCAGATCAGCAACTCACAGCTTGGAGTTATGACCAGCTACCCAAAGACTCCTCCCTGGGGCCTGGGAG GGGGGAGGGTCCTCCAACCCCTCCCTCCCAACCACCTCTGTCGCCAAAGAAGTTTTCATCTTCCACAGCC AACCGAGGTCCCTGCCCCAGGGTGCAAGAGGCAAGACCTGGGGATCTGGGAAAGGTGGAAGCTCTGCTCC AGGAGGACCTGCTGCTGACGAAGCCCGAGATGTTTGAGAACCCACTGTATGGATCCGTGAGTTCCTTCCC TAAGCTGGTGCCCAGGAAAGAGCAGGAGTCTCCCAAGATGCTGCGGAAGGAGCCCCCGCCCTGTCCAGAC CCAGGAATCTCATCACCCAGCATCGTGCTCCCCAAAGCCCAAGAGGTGGAGAGTGTCAAGGGGACAAGCA AACAGGCCCCTGTGCCTGTCCTTGGCCCCACACCCCGGATCCGCTCCTTTACCTGTTCTTCTTCTGCTGA GGGCAGAATGACCAGTGGGGACAAGAGCCAAGGGAAGCCCAAGGCCTCAGCCAGTTCCCAAGCCCCAGTG CCAGTCAAGAGGCCTGTCAAGCCTTCCAGGTCAGAAATGAGCCAGCAGACAACACCCATCCCAGCTCCAC GGCCACCCCTGCCAGTCAAGAGTCCTGCTGTCCTGCAGCTGCAACATTCCAAAGGCAGAGACTACCGTGA CAACACAGAACTCCCCCACCATGGCAAGCACCGCCAAGAGGAGGGGCTGCTTGGCAGGACTGCCATGCAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211781 representing NM_010566
Red=Cloning site Green=Tags(s) MPAMVPGWNHGNITRSKAEELLSRAGKDGSFLVRASESIPRAYALCVLFRNCVYTYRILPNEDDKFTVQA SEGVPMRFFTKLDQLIDFYKKENMGLVTHLQYPVPLEEEDAIDEAEEDTESVMSPPELPPRNIPMSAGPS EAKDLPLATENPRAPEVTRLSLSETLFQRLQSMDTSGLPEEHLKAIQDYLSTQLLLDSDFLKTGSSNLPH LKKLMSLLCKELHGEVIRTLPSLESLQRLFDQQLSPGLRPRPQVPGEASPITMVAKLSQLTSLLSSIEDK VKSLLHEGSESTNRRSLIPPVTFEVKSESLGIPQKMHLKVDVESGKLIVKKSKDGSEDKFYSHKKILQLI KSQKFLNKLVILVETEKEKILRKEYVFADSKKREGFCQLLQQMKNKHSEQPEPDMITIFIGTWNMGNAPP PKKITSWFLSKGQGKTRDDSADYIPHDIYVIGTQEDPLGEKEWLELLRHSLQEVTSMTFKTVAIHTLWNI RIVVLAKPEHENRISHICTDNVKTGIANTLGNKGAVGVSFMFNGTSLGFVNSHLTSGSEKKLRRNQNYMN ILRFLALGDKKLSPFNITHRFTHLFWLGDLNYRVELPTWEAEAIIQKIKQQQYSDLLAHDQLLLERKDQK VFLHFEEEEITFAPTYRFERLTRDKYAYTKQKATGMKYNLPSWCDRVLWKSYPLVHVVCQSYGSTSDIMT SDHSPVFATFEAGVTSQFVSKNGPGTVDSQGQIEFLACYATLKTKSQTKFYLEFHSSCLESFVKSQEGEN EEGSEGELVVRFGETLPKLKPIISDPEYLLDQHILISIKSSDSDESYGEGCIALRLETTEAQHPIYTPLT HHGEMTGHFRGEIKLQTSQGKMREKLYDFVKTERDESSGMKCLKNLTSHDPMRQWEPSGRVPACGVSSLN EMINPNYIGMGPFGQPLHGKSTLSPDQQLTAWSYDQLPKDSSLGPGRGEGPPTPPSQPPLSPKKFSSSTA NRGPCPRVQEARPGDLGKVEALLQEDLLLTKPEMFENPLYGSVSSFPKLVPRKEQESPKMLRKEPPPCPD PGISSPSIVLPKAQEVESVKGTSKQAPVPVLGPTPRIRSFTCSSSAEGRMTSGDKSQGKPKASASSQAPV PVKRPVKPSRSEMSQQTTPIPAPRPPLPVKSPAVLQLQHSKGRDYRDNTELPHHGKHRQEEGLLGRTAMQ TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_010566 |
ORF Size | 4937 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010566.1 |
RefSeq Size | 4865 bp |
RefSeq ORF | 3576 bp |
Locus ID | 16331 |
UniProt ID | Q9ES52 |
Gene Summary | Phosphatidylinositol (PtdIns) phosphatase that specifically hydrolyzes the 5-phosphate of phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) to produce PtdIns(3,4)P2, thereby negatively regulating the PI3K (phosphoinositide 3-kinase) pathways (By similarity). Able also to hydrolyzes the 5-phosphate of phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P3) and inositol 1,3,4,5-tetrakisphosphate (PubMed:9367159). Acts as a negative regulator of B-cell antigen receptor signaling. Mediates signaling from the FC-gamma-RIIB receptor (FCGR2B), playing a central role in terminating signal transduction from activating immune/hematopoietic cell receptor systems. Acts as a negative regulator of myeloid cell proliferation/survival and chemotaxis, mast cell degranulation, immune cells homeostasis, integrin alpha-IIb/beta-3 signaling in platelets and JNK signaling in B-cells. Regulates proliferation of osteoclast precursors, macrophage programming, phagocytosis and activation and is required for endotoxin tolerance. Involved in the control of cell-cell junctions, CD32a signaling in neutrophils and modulation of EGF-induced phospholipase C activity. Key regulator of neutrophil migration, by governing the formation of the leading edge and polarization required for chemotaxis. Modulates FCGR3/CD16-mediated cytotoxicity in NK cells. Mediates the activin/TGF-beta-induced apoptosis through its Smad-dependent expression.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
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SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC223881 | Inpp5d (untagged) - Mouse inositol polyphosphate-5-phosphatase D (Inpp5d), transcript variant 1, (10ug) |
CNY 15,300.00 |
|
MG226128 | Inpp5d (tGFP-tagged) - Mouse inositol polyphosphate-5-phosphatase D (Inpp5d) transcript variant 1, (10ug) |
CNY 11,500.00 |
|
MR226128 | Inpp5d (Myc-DDK-tagged) - Mouse inositol polyphosphate-5-phosphatase D (Inpp5d), transcript variant 1 |
CNY 8,296.00 |
|
MR226128L3 | Lenti ORF clone of Inpp5d (Myc-DDK-tagged) - Mouse inositol polyphosphate-5-phosphatase D (Inpp5d), transcript variant 1 |
CNY 12,350.00 |
|
MR226128L4 | Lenti ORF clone of Inpp5d (mGFP-tagged) - Mouse inositol polyphosphate-5-phosphatase D (Inpp5d), transcript variant 1 |
CNY 12,350.00 |