Atn1 (BC050920) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	atrophin-1, Atr1
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211755 representing BC050920 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGACACGACAGAATAAAGACTCGATGTCAATGAGGAGTGGACGGAAGAAAGAGGCCCCCGGGCCCC GGGAAGAGCTGAGATCAAGGGGCCGGGCCTCCCCTGGAGGGGTCAGCACATCCAGCAGTGATGGCAAAGC TGAGAAGTCCAGGCAGACAGCCAAGAAGGCCCGGATAGAGGAGCCCTCTGCCCCAAAGGCCAGCAAGCAG GGCCGGAGCGAGGAGATCTCAGAGAGTGAGAGCGAGGAGACCAGTGCGCCCAAAAAGACCAAAACCGAGC AGGAGCTCCCTCGCCCGCAGTCTCCCTCGGATCTGGACAGCTTGGATGGGCGCAGCATTAACGATGACGG CAGCAGCGACCCTAGAGATATAGACCAGGACAACCGAAGCACATCCCCCAGCATCTACAGCCCGGGCAGC GTGGAAAATGACTCGGACTCATCCTCTGGCCTGTCCCAGGGCCCCGCCCGCCCCTACCACCCACCTCCAC TCTTCCCTCCTTCCCCTCCACCACCAGACAGCACTCCCCGACAGCCAGAGTCTGGCTTTGAACCTCATCC TTCTGTGCCGCCTACTGGATATCATGCTCCGATGGAGCCCCCCACATCGAGATTATTCCAGGGCCCACCA CCTGGAGCTCCTCCCACACACCCACAGCTCTACCCTGGGAATGCTAGTGGAGGTGTTTTATCTGGACCCC CCATGGGTCCCAAAGGGGGAGCCGCTGCCTCCTCAGTGGGTGCCCCTAGCGGAGGCAAGCAACACCCCCC ACCCACTACCCCAATTCCAATATCAAGTTCTGGGGCCAGTGGTGCTCCTCCAGCAAAGCCACCCAGTGCT CCAGTGGGTGGTGGGAGCTTACCTTCTGCACCACCACCAGCTTCTTTCCCCCATGTGACACCAAACCTGC CTCCTCCACCTGCCCTGAGACCCCTCAACAATGCCTCAGCCTCTCCTCCTGGCATGGGGGCTCAGCCAAT CCCTGGGCATCTGCCCTCTCCCCATGCCATGGGGCAGGGCATGAGTGGACTTCCTCCTGGCCCAGAGAAG GGTCCAACCCTGGCCCCTTCTCCCCACCCTTTGCCCCCAGCTTCTTCCTCTGCCCCTGGGCCTCCAATGC GATATCCATATTCATCCTCCAGTAGCTCTGCCGCAGCCTCTTCTAGTTCCTCCTCCTCCTCTGCCTCCCA GTACCCTGCTTCCCAGGCCCTGCCCAGTTATCCTCATTCCTTCCCCCCACCAACTAGTATGTCTGTCTCT AATCAGCCACCCAAGTACACCCAGCCTTCTCTCCCATCCCAAGCTGTGTGGAGCCAGGGTCCACCTCCTC CTCCTCCCTATGGCCGCCTCTTGGCCAACAACAACACCCATCCAGGCCCTTTCCCTCCTACTGGGGGTCA ATCTACAGCCCACCCAGCAGCCCCTACACATCACCATCACCAGCAGCAGCCACAGCAACAACATCATCAT GGAAACTCTGGGCCCCCTCCACCCGGAGCGTATCCTCACCCTCTAGAGAGCAGTAACTCCCATCATGCAC ACCCTTACAACATGTCACCCTCCCTGGGGTCTTTAAGGCCCTACCCCCCAGGGCCAGCACACCTGCCTCC ACCTCATGGCCAGGTGTCCTATAACCAAGCAGGTCCCAATGGTCCCCCAGTTTCTTCTTCCAACTCTTCC GGGTCTTCCTCTCAAGCCTCCTATTCATGTTCACACCCCTCTTCATCCCAGGGCCCCCAAGGAGCATCCT ACCCCTTCCCACCAGTCCCTCCAGTCACCACCTCCTCAGCTACCCTTTCCACTGTCATCGCCACCGTGGC TTCCTCGCCAGCAGGCTACAAAACAGCTTCGCCACCTGGGCCCCCTCAGTACAGCAAGAGAGCCCCATCC CCAGGGTCCTACAAGACAGCCACCCCGCCTGGATACAAACCGGGGTCACCACCCTCCTTCAGAACAGGGA CCCCACCCGGCTATCGAGGCACCTCTCCGCCAGCAGGCCCAGGGACCTTCAAACCAGGTTCACCGACCGT GGGGCCGGGGCCCCTGCCACCCGCGGGGCCTTCAAGTTTGTCATCTCTGCCTCCGCCACCTGCGGCCCCG ACTACAGGGCCGCCCCTGACCGCCACGCAGATCAAACAGGAGCCGGCGGAAGAGTATGAACCTCCCGAGA GTCCGGTGCCTCCGGCCCGCAGCCCCTCGCCCCCTCCCAAGGTGGTGGACGTGCCCAGCCATGCCAGCCA GTCAGCCAGGTTCAATAAGCACTTGGACCGCGGCTTCAACTCGTGCGCGCGCAGCGACCTGTACTTCGTG CCGCTGGAGGGCTCCAAGCTGGCCAAGAAGCGCGCGGACCTGGTGGAGAAAGTGCGGCGCGAGGCCGAGC AGCGCGCGCGCGAGGAGAAAGAGCGCGAGCGCGAGCGGGAACGCGAAAAGGAGCGCGAGCGCGAGAAAGA GCGCGAGCTGGAGCGCAGTGTGAAACTGGCCCAGGAGGGCCGTGCTCCAGTGGAGTGCCCATCTCTGGGT CCAGTGCCCCATCGGCCTCCCTTTGAGCCTGGCAGCGCTGTGGCTACAGTGCCCCCTTACCTGGGTCCTG ATACTCCGGCCTTGCGCACTCTCAGTGAATACGCCCGACCTCATGTCATGTCTCCTGGCAATCGCAACCA CCCATTCTATGTGCCCTTGGGGGCAGTGGACCCGGGGCTTCTGGGTTACAATGTCCCAGCCCTGTACAGC AGCGACCCAGCTGCCCGAGAACGGGAGCGGGAAGCCCGTGAACGTGACCTCCGTGACCGGCTCAAGCCTG GCTTTGAGGTGAAACCTAGTGAGCTGGAACCCCTACATGGGGTTCCCGGGCCAGGCCTGGATCCCTTCCC CCGACACGGGGGCCTGGCTCTACAGCCCGGGCCACCTGGCCTGCATCCTTTCCCTTTTCATCCGAGCCTG GGGCCCCTGGAACGAGAACGGCTAGCGCTGGCAGCTGGGCCAGCCTTGCGTCCTGACATGTCTTATGCTG AGCGGTTGGCAGCTGAAAGGCAGCATGCAGAAAGGGTGGCAGCCCTGGGCAATGATCCACTAGCCCGGCT GCAGATGCTCAACGTGACTCCCCATCACCACCAGCACTCCCACATCCACTCTCACCTTCACCTGCACCAG CAGGATGCTATCCACGCAGCCTCTGCCTCGGTGCACCCTCTCATTGACCCCCTGGCCTCAGGGTCTCACC TTACCCGGATCCCCTACCCAGCTGGGACCCTCCCCAACCCCCTTCTTCCTCACCCTCTGCACGAGAACGA AGTTCTTCGTCACCAGCTTTTTGCTGCCCCTTACCGGGACCTGCCGGCCTCCCTTTCTGCTCCAATGTCA GCGGCTCATCAGCTGCAGGCCATGCACGCGCAGTCAGCTGAGCTGCAGCGCTTGGCGCTGGAACAGCAGC AGTGGCTACATGCTCATCACCCATTGCACAGCGTGCCACTACCTGCCCAGGAAGACTACTACAGTCACCT GAAGAAGGAGAGTGACAAGCCGCTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211755 representing BC050920 Red=Cloning site Green=Tags(s) MKTRQNKDSMSMRSGRKKEAPGPREELRSRGRASPGGVSTSSSDGKAEKSRQTAKKARIEEPSAPKASKQ GRSEEISESESEETSAPKKTKTEQELPRPQSPSDLDSLDGRSINDDGSSDPRDIDQDNRSTSPSIYSPGS VENDSDSSSGLSQGPARPYHPPPLFPPSPPPPDSTPRQPESGFEPHPSVPPTGYHAPMEPPTSRLFQGPP PGAPPTHPQLYPGNASGGVLSGPPMGPKGGAAASSVGAPSGGKQHPPPTTPIPISSSGASGAPPAKPPSA PVGGGSLPSAPPPASFPHVTPNLPPPPALRPLNNASASPPGMGAQPIPGHLPSPHAMGQGMSGLPPGPEK GPTLAPSPHPLPPASSSAPGPPMRYPYSSSSSSAAASSSSSSSSASQYPASQALPSYPHSFPPPTSMSVS NQPPKYTQPSLPSQAVWSQGPPPPPPYGRLLANNNTHPGPFPPTGGQSTAHPAAPTHHHHQQQPQQQHHH GNSGPPPPGAYPHPLESSNSHHAHPYNMSPSLGSLRPYPPGPAHLPPPHGQVSYNQAGPNGPPVSSSNSS GSSSQASYSCSHPSSSQGPQGASYPFPPVPPVTTSSATLSTVIATVASSPAGYKTASPPGPPQYSKRAPS PGSYKTATPPGYKPGSPPSFRTGTPPGYRGTSPPAGPGTFKPGSPTVGPGPLPPAGPSSLSSLPPPPAAP TTGPPLTATQIKQEPAEEYEPPESPVPPARSPSPPPKVVDVPSHASQSARFNKHLDRGFNSCARSDLYFV PLEGSKLAKKRADLVEKVRREAEQRAREEKEREREREREKEREREKERELERSVKLAQEGRAPVECPSLG PVPHRPPFEPGSAVATVPPYLGPDTPALRTLSEYARPHVMSPGNRNHPFYVPLGAVDPGLLGYNVPALYS SDPAAREREREARERDLRDRLKPGFEVKPSELEPLHGVPGPGLDPFPRHGGLALQPGPPGLHPFPFHPSL GPLERERLALAAGPALRPDMSYAERLAAERQHAERVAALGNDPLARLQMLNVTPHHHQHSHIHSHLHLHQ QDAIHAASASVHPLIDPLASGSHLTRIPYPAGTLPNPLLPHPLHENEVLRHQLFAAPYRDLPASLSAPMS AAHQLQAMHAQSAELQRLALEQQQWLHAHHPLHSVPLPAQEDYYSHLKKESDKPL TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC050920
ORF Size	3527 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC050920, AAH50920
RefSeq Size	4421 bp
RefSeq ORF	3527 bp
Locus ID	13498
Gene Summary	Transcriptional corepressor. Corepressor of MTG8 transcriptional repression. Has some intrinsic repression activity which is independent of the number of the poly-Q repeats (By similarity). Recruits NR2E1 to repress transcription. Promotes vascular smooth cell (VSMC) migration and orientation.[UniProtKB/Swiss-Prot Function]

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