Atp13a2 (BC042661) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	1110012E06Rik; AA589443
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211743 representing BC042661 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGCAGACAGCAGCCTGCTCATGGGCAGCACGCCCCCCAGCTATGGGACCCTGACGACAGGGACGT CAATCGATCCCCTCAGCTCCTCAGCTTCATCCGTGAGGCTCAGCGGCTACTGTGGCAGTCCATGGAGGGC CATCGGCTATCACGCCGCGGTCTGGATGCTGGCCGGGATCCCTTGGCTGCTATTCCGCTGGAAGCCCTTG TGGGGCGTGCGCCTGCGCCTGAAGCCATGCAGCCTGGCCCACGCCGAAACACTCGTTATAGAAATAAAAG ACAAAGAGGGTAGCTCCAGACAGCTCTTCACGGTTCAGGTGCAGACTGAGGCTCTTGTCCAGGGCAGCCT GGAGCTGCCCCCACAGGCCCAGGCAGAGGATGGCCGGAGCCAGGCAGCCGTGGGAGTGACACCAGAGGGC ACATGGCAGGACACCTCCGAACTCCACAGGCAGGAAGAGGCAAAGCAGGTACTGCGCTATTATGTCCTCC AAGGTCAACGCTACGTCTGGATGGAAACCCAGCAAGCCTTCTGCCAAGTCAGCCTGCTGGACCACAGTCG TACCTGTGATGACGTCCACTGCTCCAGCTCCGGCCTCAGCCTCCAAGACCAAGCTACGAGGAAGACCATT TACGGCCCCAATGTGATCAGTATTCCTGTCAAGTCCTACCTGCAGCTGCTGGCGGATGAGGCCCTGAATC CCTACTATGGGTTCCAAGCCTTCAGCATCGCACTGTGGCTGGCCGACCACTACTACTGGTATGCTCTCTG CATCTTCCTCATCTCAGCCATCTCCATCTGCCTGGCTCTGTACAAGACCAGAAAGCAAAGCTTGACCCTG AGGGACATGGTCAAGCTGTCCGTGCGGGTGCAGGTGTGCCGACCCGGAGGAGAGGAAGAGTGGGTGGACT CCAGTGAGCTGGTGCCCGGAGACTGCCTGGTGCTGCCCCAGGAGGGCGGGGTGATGCCATGCGACGCGGC TCTGGTGGCCGGGGAGTGTGTGGTCAACGAGAGCTCCCTGACAGGGGAGAGCACCCCTGTGCTGAAGACG GCCCTGCCAGAGGGGCCCAAGCCCTACTGCCCAGAGACCCACCGGCGGCACACCCTCTTCTGTGGCACCC TCATTCTGCAGGCCCGAGCCTACGTGGGACCCCGTGTGCTAGCAGTGGTGACTCGGACAGGGTTCTGCAC AGCCAAAGGGGGCCTGGTGAGCTCCATCCTACACCCAAGGCCCATCAGCTTCAAGTTCTACAAACACAGC ATGAAGTTTGTGGCTGCGCTCTCCGTCCTGGCTCTGCTCGGCACCGTCTACAGCATCGTCATCCTCTACC GAAACCGGGTACCTGTGAGGGAGATTGTGATCCGAGCTCTGGACCTGGTGACGGTGGTGGTGCCGCCCGC CCTGCCTGCCGCCATGACGGTGTGCACACTCTATGCCCAGAGTCGGCTGCGCGCACAGGGCATCTTCTGC ATCCACCCACTGCGCATCAACTTGGGGGGCAAGCTGCGGCTCGTGTGCTTTGACAAGACAGGCACCCTCA CAGAGGACGGCTTGGACGTAATGGGCGTAGTGCCCCTAAAGGGGCAGGTGTTGCTGCCACTGGTCCCAGA GCCCCGCCACCTGCCCCTGGGGCCCCTCCTGCGAGCACTGGCCACCTGTCATGCCCTCAGCCAGCTACAT GACACCCCGGTGGGCGACCCCATGGATCTGAAGATGGTGGAGTCTACAGGCTGGGTCTTAGAGGAGGGTC CAGCTGCAGGCTCAGCACCTGGGAGCCAGGTCTTGGTGGTAATGAGACCCCCACCCGGGGGCCCTCGGCA GCAGGAAGAGCCACCAGTGCCAGTCAGCGTCCTCTGCCGCTTCCCCTTCTCCTCTGCCCTGCAGCGGATG GATGTGGTAGTGACATGGCCAGGGGCCACCCAGCCTGAGGCCTACGTCAAAGGCTCCCCAGAGCTTGTGG CCAGCCTCTGCAGCCCCGAGACAGTACCCAGCGACTTTTCTCAGGTGCTGCAGAGCTACACAGCTGCTGG CTACCGAGTTGTGGCCCTGGCTGGCAAGCCACTGCCCATTGCGCCCAGCCTTGAGGCCGCTCAGCAGCTG ACAAGGGACACTGTGGAGAGGGAACTGAGCCTCCTGGGGCTGCTGGTCATGCGGAACCTACTGAAGCCAC AGACGGCCCCAGTTATCCAGACTCTGAGGAAGACGGGCATCCGCACCGTTATGGTGACAGGGGACAACCT GCAGACAGCAGTCACCGTGGCCAGAGCTTGTGGCATGGTGGGCGCCCAGGAGCACCTGGCTGTCATCCAT GCCACCCACCCCGAGCAGGGCCAGCCTGCTGCCCTTGAGTTCTTGCCAACGGAGTCCTCTGCAGTCATGA ATGGGGCGAAGGCCACAGGCTATCCCACGGTGCCGGAACCCCAGTTCTGTCACCTGGCCCTCAGCGGGTC CACGTTTGCAGTCCTTCGGAAGCACTTCCCCAAGCTGCTGCCCAAGGTCCTGGTGCAGGCTACCGTCTTT GCCCGCATGGCCCCGGAGCAGAAGACAGAGCTAGTGTGCGAACTGCAGAGGCTTCAGTACTGTGTGGGCA TGTGCGGGGATGGAGCCAACGACTGTGGGGCCCTGAAGGCGGCTGATGTGGGCATATCGCTGTCCCAGGC GGAGGCGTCAGTGGTCTCTCCGTTCACCTCCAGCATGGCCAGTATTGAATGTGTGCCCACTGTCATCAGG GAAGGCCGCTGCTCCCTGGATACTTCATTCAGCGTCTTCAAGTACATGGCCCTCTACAGCCTGACTCAGT TCATCTCTGTGCTCATTCTCTACACAATCAACACCAACCTGGGAGACCTACAGTTCTTGGCCATTGACCT GGTCATCACCACCACGGTTGCGGTGCTCATGAGCCGCACAGGCCCTGCACTGACCTTGGTGCGAGCGCGG CCACCAGGGGCGCTGCTGAGTGTGCCTGTGCTTGGCAGTCTGCTGCTGCAGGTGGCCTTGGTGGCCGGCA TCCAGCTGGGGGGCTACTTTTTGGTCATAGCTCAGCCCTGGTTTGTGCCTCTGAACAGAACTGTGCCCGC TCCGGACAACCTGCCCAACTACGAGAATACGGTGGTCTTCTCTCTGTCTGGCTTCCAGTACCTCATCCTG GCCGCAGCGGTGTCTAAGGGGGCACCCTTCCGCCAGCCACTCTACACCAACGTGCCCTTCCTGGTGGCCC TGGCGCTCTTGGGCTCTGTCCTGGTGGGCCTCATCCTGGTCCCCGGCCTCCTGCAGGGGCCCCTAGGACT GAGGAACATTGTGGACAGCTCCTTCAAGCTACTGCTGCTGGGTCTGGTCGCCTTCAACTTCGTCGGCGCC TTTATGCTGGAGAGCGTGCTGGACCAGTGTCTCCCAGCCTGCCTGCGGTGGCTGCGGCCTAAACGGGCCT CCAAGAAGCAATTCAAGCGGCTGCAACAGGAGCTTGCAGAACATCCATGGCCCACTCTGCCCGTTGGCTC CGTGAGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211743 representing BC042661 Red=Cloning site Green=Tags(s) MSADSSLLMGSTPPSYGTLTTGTSIDPLSSSASSVRLSGYCGSPWRAIGYHAAVWMLAGIPWLLFRWKPL WGVRLRLKPCSLAHAETLVIEIKDKEGSSRQLFTVQVQTEALVQGSLELPPQAQAEDGRSQAAVGVTPEG TWQDTSELHRQEEAKQVLRYYVLQGQRYVWMETQQAFCQVSLLDHSRTCDDVHCSSSGLSLQDQATRKTI YGPNVISIPVKSYLQLLADEALNPYYGFQAFSIALWLADHYYWYALCIFLISAISICLALYKTRKQSLTL RDMVKLSVRVQVCRPGGEEEWVDSSELVPGDCLVLPQEGGVMPCDAALVAGECVVNESSLTGESTPVLKT ALPEGPKPYCPETHRRHTLFCGTLILQARAYVGPRVLAVVTRTGFCTAKGGLVSSILHPRPISFKFYKHS MKFVAALSVLALLGTVYSIVILYRNRVPVREIVIRALDLVTVVVPPALPAAMTVCTLYAQSRLRAQGIFC IHPLRINLGGKLRLVCFDKTGTLTEDGLDVMGVVPLKGQVLLPLVPEPRHLPLGPLLRALATCHALSQLH DTPVGDPMDLKMVESTGWVLEEGPAAGSAPGSQVLVVMRPPPGGPRQQEEPPVPVSVLCRFPFSSALQRM DVVVTWPGATQPEAYVKGSPELVASLCSPETVPSDFSQVLQSYTAAGYRVVALAGKPLPIAPSLEAAQQL TRDTVERELSLLGLLVMRNLLKPQTAPVIQTLRKTGIRTVMVTGDNLQTAVTVARACGMVGAQEHLAVIH ATHPEQGQPAALEFLPTESSAVMNGAKATGYPTVPEPQFCHLALSGSTFAVLRKHFPKLLPKVLVQATVF ARMAPEQKTELVCELQRLQYCVGMCGDGANDCGALKAADVGISLSQAEASVVSPFTSSMASIECVPTVIR EGRCSLDTSFSVFKYMALYSLTQFISVLILYTINTNLGDLQFLAIDLVITTTVAVLMSRTGPALTLVRAR PPGALLSVPVLGSLLLQVALVAGIQLGGYFLVIAQPWFVPLNRTVPAPDNLPNYENTVVFSLSGFQYLIL AAAVSKGAPFRQPLYTNVPFLVALALLGSVLVGLILVPGLLQGPLGLRNIVDSSFKLLLLGLVAFNFVGA FMLESVLDQCLPACLRWLRPKRASKKQFKRLQQELAEHPWPTLPVGSVR TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC042661
ORF Size	3509 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC042661, AAH42661
RefSeq Size	3953 bp
RefSeq ORF	3509 bp
Locus ID	74772
Gene Summary	ATPase that plays a role in intracellular cation homeostasis and the maintenance of neuronal integrity. Required for a proper lysosomal and mitochondrial maintenance.[UniProtKB/Swiss-Prot Function]

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