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Spag5 (NM_017407) Mouse Tagged ORF Clone
CAT＃: MG211734
- TrueORF®
Spag5 (tGFP-tagged) - Mouse sperm associated antigen 5 (Spag5)
ORF Plasmid: DDK
"NM_017407" in other vectors (4)
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CNY 9,220.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | AI874642; D11Bhm180e; Deepest; MAP126; Mastrin; S17 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211734 representing NM_017407
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGGAGGGTGAAAACACTGAATCTCGGTTTGTCGCCTTCGCCCCAAAAGGGGAAGCCAGCTATGAGTA CTCCTCTCCGAGAGCTTAAACTACAGCCCGAGGCCCTCGCCGATTCAGGGAAAGGTCCCTCTATGATCTC CGCGCTGACCCCATACCTGTGCAGGCTGGAGCTGAAGGAAAGATGCAACAACTCATCTCCAGTGGATTTT ATCAATACTGAGAACAACTTCCTTTCAGAACAGTTCAGCCATCCTTCAACGCACATAGAAGCTTGCCAGC GTGAATCCGATCCAACTCCTGAAAGCAACTCTCTTTTCCATACTTTGGAGGAAGCAATAGAAACAGTGGA TGACTTTGTCGTGGACCCAAGAGATGATAGTATAGTGGAGTCCATGGTTCTTCTGCCCTTTTCATTAGGG CAGCAGCAAGACCTGATGCTCCAGGCTCACTTAGATACCACAGCAGAGAGAACTAAAAGCTCTCTAAATG AATCTTTGGGGCTAGAAGATCTGGTGGGGAAGGAGGTGGCACCTTGTGTGGAAGACAGCCTAACAGAAAT TGTTGCTATTAGGCCCGAGCAACCTACATTTCAGGACCCTCCGTTAGGACCCAGTGACACTGAAGATGCA CCTGTGGACTTAGTTCCTTCTGAAAATGTCCTGAATTTCTCTCTGGCTCGTCTTTCTCCTTCAGCTGTCC TGGCCCAGGATTTCTCTGTCGATCATGTCGATCCAGGGGAGGAAACCGTAGAGAACAGAGTCCTACAGGA AATGGAAACGAGCTTCCCCACATTCCCTGAGGAGGCCGAATTGGGAGACCAAGCACCTGCTGCAAATGCA GAAGCCGTCTCACCCTTGTACCTGACATCAAGTCTAGTGGAGATGGGGCCCCGGGAAGCCCCAGGCCCAA CAGTAGAAGACGCCAGTAGGATTCCTGGCCTTGAGTCAGAGACTTGGATGTCCCCATTGGCCTGGCTAGA AAAGGGTGTGAATACATCGGTCATGCTACAAAACCTCCGCCAGAGCTTATCCTTTTCTTCTGTGCTTCAG GATGCTGCCGTTGGCAACACACCCCTCGCCACGTGTTCTGTGGGCACTTCCTTTACTCCTCCAGCACCAC TGGAGGTAGGCACCAAGGACAGTACTTCAGAGACAGAGCGCCTCCTCTTGGGCTGCCGTCCTCCAGATCT GGCTACCTTGTCCCGACACGACTTGGAAGAGAACCTGTTGAACTCTCTTGTCTTGCTGGAAGTTCTTTCC CACCAGCTACAAGCCTGGAAGAGCCAGTTGACTGTCCCTCACCGGGAAGCTCGAGACAGTAGTACACAGA CTGACAGCTCTCCTTGTGGGGTCACTAAGACACCTAAACATCTTCAGGACAGCAAGGAGATTAGACAGGC TCTACTGCAAGCCAGGAATGTCATGCAATCATGGGGTCTAGTCTCTGGAGACCTGTTGTCCTTGCTTCAC CTGTCCCTAACACACGTGCAAGAAGGCAGAGTGACAGTGAGTCAGGAGTCTCAGCGGTCAAAAACCCTGG TTTCCTCCTGTTCTCGTGTGCTGAAGAAATTGAAGGCAAAGCTGCAGAGCCTCAAAACAGAATGTGAGGA GGCAAGGCACAGCAAGGAAATGGCCCTTAAAGGCAAGGCTGCGGCAGAGGCAGTACTGGAGGCTTTCCGT GCACATGCCAGCCAGCGCATCAGCCAACTGGAACAGGGTCTCACATCTATGCAGGAATTCAGAGGCCTTC TACAGGAGGCACAGACCCAACTGATAGGGCTTCACACAGAACAAAAGGAGCTGGCTCAGCAGACAGTGAG TCTGAGTTCTGCCTTGCAGCAGGACTGGACATCAGTCCAATTGAATTATGGAATATGGGCAGCTTTGCTG AGTTGGTCTCGAGAACTCACCAAGAAACTCACAGCCAAGAGCCGGCAGGCCCTTCAGGAACGTGATGCTG CAATTGAAGAAAAGAAGCAGGTTGTGAAGGAAGTGGAACAAGTCTCTGCCCATTTAGAGGACTGTAAAGG TCAAATCGAACAACTGAAGTTGGAAAATAGTCGCCTTACTGCAGATCTCTCGGCTCAGCTGCAGATTCTG ACTAGCACAGAGAGTCAGCTAAAGGAAGTACGGAGTCAGCATTCCCGCTGTGTCCAGGACCTGGCCGTGA AGGATGAATTGCTGTGTCAACTTACCCAGAGCAACAAGGAGCAGGCTACTCAATGGCAAAAGGAAGAAAT GGAACTGAAACACATACAAGCAGAACTGCTACAGCAGCAGGCTGTGTTGGCTAAGGAGGTCCAGGACCTG AGGGAGACTGTGGAGTTTATAGATGAAGAAAGTCAAGTTGCTCACCGGGAGCTGGGCCAGATTGAGAGTC AGTTGAAAGTCACGCTAGAGTTACTGCGGGAGCGCAGCCTGCAGTGTGAGACCCTCAGGGACACTGTGGA CAGCCTGAGGGCTGAGCTGGCCAGCACTGAAGCAAAGCATGAGAAACAGGCCCTAGAGAAGACACACCAG CATTCTCAAGAGCTGCGGCTACTGGCTGAGCAGCTGCAGAGTCTCACCCTCTTCTTACAGGCAAAACTCA AGGAGAACAAGGCTGAATCAGAGATCATTCTGCCCAGCACAGGCTCTGCTCCAGCCCAGGAACACCCTCT GTCCAATGACAGCAGCATCTCAGAACAGACCCCGACAGCAGCAGTAGATGAAGTGCCAGAACCAGCTCCT GTGCCATTGCTTGGAAGTGTTAAGAGTGCTTTCACCCGAGTAGCCTCAATGGCTTCCTTTCAGCCTACAG AGACCCCAGACTTGGAGAAGAGCCTGGCAGAAATGAGTACTGTGTTACAAGAGCTTAAGAGCCTGTGTTC CCTGCTGCAAGAGTCTAAAGAGGAGGCCACTGGGGTCCTGCAGAGGGAAATCTGTGAACTACACTCGAGA CTACAGGCCCAAGAAGAAGAGCATCAGGAAGCCCTGAAGGCAAAGGAAGCAGACATGGAGAAGCTGAACC AGGCCTTGTGCTTGCTCCGCAAGAATGAGAAGGAGCTCCTGGAAGTGATACAGAAGCAGAACGAGAAGAT CCTGGGGCAAATAGACAAGAGCGGCCAGCTCATAAACCTCAGAGAGGAGGTGACCCAGCTCACACAGTCA CTTCGGCGTGCAGAGACAGAGACTAAAGTGCTCCAGGAAGCCCTGGAAGGCCAGCTAGATCCCAGCTGCC AGCTGATGGCTACTAACTGGATCCAGGAAAAAGTGTTTCTCTCACAGGAGGTGAGCAAGCTGAGGGTTAT GTTCCTGGAGATGAAAACTGAGAAGGAACAGCTGATGGACAAGTATCTGAGCCATAGGCACATCCTGGAG GAGAATCTTCGGCGCTCTGACACAGAGTTAAAGAAACTCGATGACACAATTCAGCATGTCTATGAGACTC TGTTGTCTATCCCAGAGACTATGAAGAGTTGCAAGGAGTTACAAGGATTGCTAGAATTTCTGAGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211734 representing NM_017407
Red=Cloning site Green=Tags(s) MWRVKTLNLGLSPSPQKGKPAMSTPLRELKLQPEALADSGKGPSMISALTPYLCRLELKERCNNSSPVDF INTENNFLSEQFSHPSTHIEACQRESDPTPESNSLFHTLEEAIETVDDFVVDPRDDSIVESMVLLPFSLG QQQDLMLQAHLDTTAERTKSSLNESLGLEDLVGKEVAPCVEDSLTEIVAIRPEQPTFQDPPLGPSDTEDA PVDLVPSENVLNFSLARLSPSAVLAQDFSVDHVDPGEETVENRVLQEMETSFPTFPEEAELGDQAPAANA EAVSPLYLTSSLVEMGPREAPGPTVEDASRIPGLESETWMSPLAWLEKGVNTSVMLQNLRQSLSFSSVLQ DAAVGNTPLATCSVGTSFTPPAPLEVGTKDSTSETERLLLGCRPPDLATLSRHDLEENLLNSLVLLEVLS HQLQAWKSQLTVPHREARDSSTQTDSSPCGVTKTPKHLQDSKEIRQALLQARNVMQSWGLVSGDLLSLLH LSLTHVQEGRVTVSQESQRSKTLVSSCSRVLKKLKAKLQSLKTECEEARHSKEMALKGKAAAEAVLEAFR AHASQRISQLEQGLTSMQEFRGLLQEAQTQLIGLHTEQKELAQQTVSLSSALQQDWTSVQLNYGIWAALL SWSRELTKKLTAKSRQALQERDAAIEEKKQVVKEVEQVSAHLEDCKGQIEQLKLENSRLTADLSAQLQIL TSTESQLKEVRSQHSRCVQDLAVKDELLCQLTQSNKEQATQWQKEEMELKHIQAELLQQQAVLAKEVQDL RETVEFIDEESQVAHRELGQIESQLKVTLELLRERSLQCETLRDTVDSLRAELASTEAKHEKQALEKTHQ HSQELRLLAEQLQSLTLFLQAKLKENKAESEIILPSTGSAPAQEHPLSNDSSISEQTPTAAVDEVPEPAP VPLLGSVKSAFTRVASMASFQPTETPDLEKSLAEMSTVLQELKSLCSLLQESKEEATGVLQREICELHSR LQAQEEEHQEALKAKEADMEKLNQALCLLRKNEKELLEVIQKQNEKILGQIDKSGQLINLREEVTQLTQS LRRAETETKVLQEALEGQLDPSCQLMATNWIQEKVFLSQEVSKLRVMFLEMKTEKEQLMDKYLSHRHILE ENLRRSDTELKKLDDTIQHVYETLLSIPETMKSCKELQGLLEFLS TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017407 |
| ORF Size | 3495 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017407.3 |
| RefSeq Size | 3822 bp |
| RefSeq ORF | 3498 bp |
| Locus ID | 54141 |
| UniProt ID | Q7TME2 |
| Gene Summary | Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase. Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. In complex with SKAP, promotes stable microtubule-kinetochore attachments. May contribute to the regulation of separase activity. May regulate AURKA localization to mitotic spindle, but not to centrosomes and CCNB1 localization to both mitotic spindle and centrosomes. Involved in centriole duplication. Required for CDK5RAP22, CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2. In non-mitotic cells, upon stress induction, inhibits mammalian target of rapamycin complex 1 (mTORC1) association and recruits the mTORC1 component RPTOR to stress granules (SGs), thereby preventing mTORC1 hyperactivation-induced apoptosis. May enhance GSK3B-mediated phosphorylation of other substrates, such as MAPT/TAU (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC202063 | Spag5 (untagged) - Mouse sperm associated antigen 5 (Spag5), (10ug) |
CNY 8,120.00 |
|
| MR211734 | Spag5 (Myc-DDK-tagged) - Mouse sperm associated antigen 5 (Spag5) |
CNY 8,112.00 |
|
| MR211734L3 | Lenti ORF clone of Spag5 (Myc-DDK-tagged) - Mouse sperm associated antigen 5 (Spag5) |
CNY 10,260.00 |
|
| MR211734L4 | Lenti ORF clone of Spag5 (mGFP-tagged) - Mouse sperm associated antigen 5 (Spag5) |
CNY 10,260.00 |
