Spag9 (BC060100) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	JLP, Jip4, Mapk8ip4, syd1, JSAP2, JSAP2a
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211730 representing BC060100 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCCTGGATGCATGCTGCTGTTTGTGTTTGGCTTTGTTGGCGGGGCGGTGGTCATTAATTCTGCGA TCTTAGTATCTCTCTCTGTTTTGCTGCTTGTGCACTTTTCTATTTCTACCGGGGTGCCAGCGCTGACGCA GAACCTACCAAGGATACTCAGAAAAGAACGTCCTATATCATTAGGGATTTTCCCTCTACCTGCTGGAGAT GGATTGCTTACACCTGACACTCAGAAAGGAGGCGAGACCCCAGGATCAGAGCAATGGAAATTTCAAGAAT TAAGTCAACCACGTTCTCATACCAGCCTGAAGGATGAACTTTCTGATATTAGTCAAGGTGGATCTAAAGC TACCACTCCAGCTTCAACAGCAAATTCAGACGTATCAGCAATTCCTCCTGATACTCCGTCAAAGGAAGAT AATGAAGGATTTGTAAAAGGCACAGATACATCAAATAAGTCAGAGATAAGCAAACACATAGAAGTCCAGG TTGCCCAAGAGACTAGGAATGTGTCTACAGAATCTGGTGAAAATGAAGAGAAATCAGAAGTTCAAGCAAT CATTGAATCCACTCCTGAATTGGATATGGACAAAGACCTCAGTGGATATAAAGGTTCAAGCACTCCCACC AAAGGCATAGAGAACAAAGCTTTTGATCGCAATACAGAATCTCTCTTTGAAGAGCTGTCCTCAGCTGGCT CGGGCCTTATTGGAGATGTGGATGAAGGAGCGGATTTACTAGGAATGGGTCGTGAGGTGGAGAATCTCAT ACTGGAGAACACACAGCTGCTGGAAACCAAAAACGCTCTGAATGTAGTGAAGAATGACTTAATAGCCAAA GTGGATGAGCTGACCTGTGAGAAGGACGTGCTGCAGGGGGAGCTGGAGGCTGTGAAGCAAGCCAAGCTGA AGCTGGAGGACAAGAACAGAGAGCTGGAGGAGGAGCTCAGGAAAGCTCGTGCAGAAGCTGAAGATGCAAG GCAAAAAGCAAAAGACGATGATGATAGTGACATTCCCACAGCCCAGAGGAAGAGGTTTACCAGAGTAGAA ATGGCCCGAGTTCTCATGGAGAGAAACCAGTACAAAGAGAGGCTGATGGAGCTTCAAGAAGCTGTTCGAT GGACGGAGATGATCCGGGCCTCACGAGAGAACCCAGCTATGCAAGAAAAGAAAAGGTCAAGCATCTGGCA ATTTTTCAGCCGACTTTTTAGCTCTTCAAGTAATGCAACAAAGAAGCCTGAACCCCCTGTTAACCTGAAG TACAACGCCCCCACCTCTCACGTGACTCCCTCCGTGAAGAAAAGGAGCAGCACTTTATCTCAGCTCCCTG GGGACAAGTCCAAGGCATTTGATTTCCTCAGTGAGGAAACTGAGGCTAGTTTAGCCTCCCGCAGAGAGCA AAAGAGAGAGCAGTATCGGCAGGTGAAGGCTCACGTTCAGAAGGAAGATGGCCGAGTGCAAGCTTTCGGC TGGAGTCTGCCTCAGAAGTACAAACAGGTAGCCAATGGTCAAGGGGAAACTAAGATGAAAAATTTACCTG TGCCTGTCTATCTCAGACCACTAGACGAAAAAGATGCGTCTATGAAGCTGTGGTGTGCTGTAGGAGTCAA TTTATCTGGTGGCAAGACAAGAGATGGCGGGTCTGTTGTTGGAGCGAGTGTATTTTACAAGGATATTGCT GGTTTGGACACTGAAGGCAGTAAACAGCGGAGTGCGTCTCAGAGTAGTTTAGACAAGTTGGATCAAGAAC TCAAGGAACAGCAGAAAGAATTTAAAAATCAAGAAGAGTTGTCCAGTCAGGTCTGGATCTGTACCAGCAC CCACTCAACTACAAAGGTTATCATCATTGATGCTGTTCAGCCTGGCAACATCCTAGATAGTTTCACTGTT TGCAATTCTCATGTTCTGTGCATTGCCAGTGTCCCAGGAGCTCGGGAAACAGACTACCCTGCAGGAGAAG AACTTTCTGAATCTGGTCAGGTAGACAAAGCGTCATTGTGTGGAAGCATGACCAGTAACAGCTCCGCAGA GATGGACAGCTTGCTGGGAGGCATCACAGTGGTTGGGTGCTCCACAGAAGGACTGACAGGAGCTGCCACT TCCCCAAGTACCAACGGTGCTTCTCCTGTGATTGAAAAGCCACCAGAAATGGAAACTGAAAATAGCGAAG TTGATGAAAATATTCCAACAGCAGAAGAAGCAACTGAAGCCACAGAGGGCAATGCAGGGTCCACTGAAGA CACTGTGGACATCTCCCAGCCTGGCGTGTACACAGAGCATGTGTTTACAGATCCACTGGGAGTTCAGATC CCAGAAGACCTCTCCCCAGTGTTTCAGTCAAGTAATGACTCAGATGTGTACAAAGATCAAATATCAGTAT TGCCAAATGAACAAGACCTTGCGAGAGAAGAAGCTCAGAAAATGAGTAGTCTTTTACCAACCATGTGGCT TGGAGCTCAGAATGGCTGTTTGTATGTCCACTCATCTGTAGCCCAGTGGAGGAAATGTCTCCATTCCATT AAGCTCAAAGACTCAATTCTCAGTATTGTACATGTGAAAGGAATTGTACTAGTGGCCCTGGCGGATGGCA CCCTTGCAATCTTCCACAGAGGAGTTGATGGACAATGGGATCTGTCAAACTATCACCTTTTAGACCTTGG ACGTCCTCATCATTCCATACGATGCATGACTGTGGTACATGACAAAGTCTGGTGTGGCTATAGGAACAAA ATCTATGTGGTTCAACCAAAGGCTATGAAGATAGAGAAATCATTTGATGCACACCCCAGGAAGGAGAGCC AAGTACGGCAGCTTGCATGGGTGGGTGATGGTGTATGGGTCTCCATTCGTTTGGATTCCACGCTCCGCCT CTATCATGCACACACATACCAACATCTCCAGGATGTGGATATTGAGCCTTATGTAAGCAAAATGTTAGGT ACTGGAAAACTGGGTTTCTCTTTCGTGAGGATAACAGCTCTTATGGTGTCTTGCAATCGTTTGTGGGTGG GGACTGGAAATGGTGTCATTATCTCCATCCCACTGACAGAAACAAATAAAACCTCAGGGACTCCAGGTAA CCGTCCTGGAAGTGTAATCCGTGTATATGGTGATGAGAACAGTGATAAAGTGACTCCAGGGACATTTATA CCCTACTGTTCAATGGCACATGCACAGCTTTGTTTCCACGGGCACCGGGATGCTGTGAAATTCTTTGTGG CAGTCCCAGGTCAGGTCATTAGCCCACAAAGTAGCAGTGGTGGGGCAGATCTTACAGCTGACAAGGCAGG GTCATCTGCACAGGAGCCCAGTAGCCAGACGCCCTTGAAGTCCATGCTTGTCATCAGTGGAGGAGAGGGC TACATTGACTTCCGGATGGGTGATGAAGGTGGAGAATCTGAACTTCTGGGAGAGGATCTTCCACTGGAAC CTTCAGTCACCAAAGCCGAGAGGAGTCACCTGATAGTGTGGCAAGTGATGTGTGGCAATGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211730 representing BC060100 Red=Cloning site Green=Tags(s) MNPGCMLLFVFGFVGGAVVINSAILVSLSVLLLVHFSISTGVPALTQNLPRILRKERPISLGIFPLPAGD GLLTPDTQKGGETPGSEQWKFQELSQPRSHTSLKDELSDISQGGSKATTPASTANSDVSAIPPDTPSKED NEGFVKGTDTSNKSEISKHIEVQVAQETRNVSTESGENEEKSEVQAIIESTPELDMDKDLSGYKGSSTPT KGIENKAFDRNTESLFEELSSAGSGLIGDVDEGADLLGMGREVENLILENTQLLETKNALNVVKNDLIAK VDELTCEKDVLQGELEAVKQAKLKLEDKNRELEEELRKARAEAEDARQKAKDDDDSDIPTAQRKRFTRVE MARVLMERNQYKERLMELQEAVRWTEMIRASRENPAMQEKKRSSIWQFFSRLFSSSSNATKKPEPPVNLK YNAPTSHVTPSVKKRSSTLSQLPGDKSKAFDFLSEETEASLASRREQKREQYRQVKAHVQKEDGRVQAFG WSLPQKYKQVANGQGETKMKNLPVPVYLRPLDEKDASMKLWCAVGVNLSGGKTRDGGSVVGASVFYKDIA GLDTEGSKQRSASQSSLDKLDQELKEQQKEFKNQEELSSQVWICTSTHSTTKVIIIDAVQPGNILDSFTV CNSHVLCIASVPGARETDYPAGEELSESGQVDKASLCGSMTSNSSAEMDSLLGGITVVGCSTEGLTGAAT SPSTNGASPVIEKPPEMETENSEVDENIPTAEEATEATEGNAGSTEDTVDISQPGVYTEHVFTDPLGVQI PEDLSPVFQSSNDSDVYKDQISVLPNEQDLAREEAQKMSSLLPTMWLGAQNGCLYVHSSVAQWRKCLHSI KLKDSILSIVHVKGIVLVALADGTLAIFHRGVDGQWDLSNYHLLDLGRPHHSIRCMTVVHDKVWCGYRNK IYVVQPKAMKIEKSFDAHPRKESQVRQLAWVGDGVWVSIRLDSTLRLYHAHTYQHLQDVDIEPYVSKMLG TGKLGFSFVRITALMVSCNRLWVGTGNGVIISIPLTETNKTSGTPGNRPGSVIRVYGDENSDKVTPGTFI PYCSMAHAQLCFHGHRDAVKFFVAVPGQVISPQSSSGGADLTADKAGSSAQEPSSQTPLKSMLVISGGEG YIDFRMGDEGGESELLGEDLPLEPSVTKAERSHLIVWQVMCGNE TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC060100
ORF Size	3492 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC060100, AAH60100
RefSeq Size	4221 bp
RefSeq ORF	3494 bp
Locus ID	70834
Gene Summary	The JNK-interacting protein (JIP) group of scaffold proteins selectively mediates JNK signaling by aggregating specific components of the MAPK cascade to form a functional JNK signaling module (PubMed:12391307, PubMed:15767678). Regulates lysosomal positioning by acting as an adapter protein which links PIP4P1-positive lysosomes to the dynein-dynactin complex (By similarity).[UniProtKB/Swiss-Prot Function]

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