Mtmr3 (BC081544) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	FYVE-DSP1, ZFYVE10, mKIAA0371
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211725 representing BC081544 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATGAAGAGATGCGGCATAGCCTTGAGTGCATCCAGGCCAATCAGATCTTTCCCAGGAAGCAGCTGA TTCGGGAGGATGAGAATCTTCAGGTTCCTTTCCTTGAACTTCATGGAGAGAGCACAGAGTATGTGGGCCG TGCTGAGGAGGCCATCATTGCTCTTTCTAATTACAGGCTTCACATCAAGTTCAAGGAGTCTCTTGTCAAT GTTCCATTACAGCTTATTGAAAGCGTTGAATGCCGTGATATATTTCAGCTTCATTTGACATGCAAAGACT GCAAAGTTATCAGGTGTCAGTTCCCAACCTTTGAGCAATGTCAAGATTGGCTTAAGAGATTGAACAATGC AATCCGACCACCTGGTAAAATAGAAGATCTCTTCTCATTTGCATACCATGCTTGGTGCATGGAGGTCTAC GCCAGTGAAAAGGAGCAGCATGGAGACCTGTGCAGACCAGGGGAACATGTGACGTCAAGGTTTAAGAATG AAGTGGAGCGGATGGGTTTTGATATGAACAATGCCTGGAGGATTTCCAACATCAATGAGAAATACAAATT ATGTGGTAGTTATCCACAAGAGCTCATAGTGCCTGCTTGGATCACTGACAAAGAGCTGGAAAGCGTGGCA GGCTTCAGATCCTGGAAGCGCATCCCTGCTGTCATCTACAGGCACCAGAGCAATGGAGCTGTCATTGCCC GCTGCGGACAACCGGAGGTTAGCTGGTGGGGCTGGCGAAATGCTGATGATGAACATCTGGTGCAGTCAGT GGCCAGAGCCTGTGCTTCTGACTCTCAGTCAAGTATCAGCAAGGTTTCCACTCGGAACAGTTGTCGGGAC TTTCCCAACGCAGGGGATCTTTCAGATGTGGAGTTCGATTCCTCTCTGTCAAATACTTCAGGAGCAGAAA GTTTAGCTCTCCAGCCCCAGAAGCTTTTGATCTTGGATGCACGCTCCTATGCAGCAGCTGTGGCAAATCG AGCCAAAGGAGGAGGCTGTGAGTGCCCAGAGTATTATCCTAACTGTGAAGTTGTGTTTATGGGAATGGCA AACATACATTCTATCCGGAGGAGTTTCCAGTCTCTGCGGCTGCTGTGCACACAGATGCCAGATCCTGGAA ATTGGCTTTCTGCCCTTGAAAGTACAAAGTGGCTCCATCACCTATCTGTGCTTCTGAAGTCGGCACTTCT CGTGGTGCATGCTGTGGACCGTGATCAGCGACCAGTGCTAGTGCACTGCTCCGATGGCTGGGATCGGACT CCCCAGATTGTGGCCCTAGCTAAGCTCCTACTTGACCCTTATTACCGAACCATAGAGGGTTTCCAAGTCC TGGTGGAGATGGAATGGCTCGATTTTGGCCATAAGTTTGCTGACCGGTGTGGTCATGGGGAGGACTCAGA TGATCTGAATGAGCGCTGCCCAGTGTTTCTACAATGGCTTGACTGTGTTCATCAACTTCAGAGGCAGTTT CCTTGCTCTTTTGAGTTCAACGAAGCATTCCTTGTGAAACTGGTACAGCATACCTATTCCTGTCTCTTTG GAACATTCCTGTGCAACAACGCCAAAGAGAGAGGGGAAAAGCAGACTCAGGAACGGACATGCTCTGTGTG GTCACTTCTTCGAGCAGGCAACAAGGCTTTCAAAAACCTACTGTATTCATCTCAGTCAGAAGCCGTGCTC TACCCCGTGTGCCATGTGCGGAATCTGATGCTGTGGAGCGCAGTGTACCTTCCCTGCCCATCCCCATCTA CCCCCACAGATGACAGCTGTGCACCATACCCAGTCCCAGGCACTAGCCCTGATGAGCCCCCACTGAGCCG GCTACCCAAGACTAGATCATTTGACAATCTGACCACAACCTGCGAAAACATGGTGCCGCTGGCCAGCCGG CGCAGCAGTGACCCCAGCCTGAATGAGAAGTGGCAGGAGCACGGGCGCTCGCTGGAGCTGAGCAGCTTTG CCAGCGCTGGGGAAGAGGTGCCTGCTATGGACAGCCTGCGCAAGCCCAGCAGGCTGCTCGGAGGTGCTGA GCTTTCTGTGGCAGCCGGAGTGGCTGAAGGGCAGATGGAGAACATTTTGCAAGAGGCCACCAAAGAGGAG AGTGGGGTAGAAGAGCCTACCCACAGGGGGCACACTGAGGTGCCAGAGGTCAAAGAGGAGGCACCCCTAG CAAAAGAAAGTAGCATGGCGGCCGAGGGCCCCGTTGTACTTTACCAAGAACCACAGCTGGATGACGCTAC TTTAAGAAGCCATCAAGGCCCCAGCCTCTCTTTGTTCTCCCAGGGTATTCCTGAACATCAGGATGGGCAC AATGTCCTCTCTAGTTCACTCCAAGCTCCTCTCAGTGGAGAGGACTCCCAGGAGGTCCCTGTAGAACAGC CTCAAGTAGAGAATATTGCAGAGGACAGGGAGAATGTAGCTCCTGCTGTCCCAGTAGATGCAAAAGTTGG CCTTGGTATCTCACAGTCTAGTTCTCTGCTGCCTTCCCAAGTCCCATTCGAGACAAGAGGACCACACATC AACAACTCCGTGCACATGTTACTCGAAGATAAGGTAAAGTCAGAAAGTGGGCCCCAGCTCCATCACAGAC CTTGCCCAGCAAGCAGTGGTAGATTCAGTGGCAAGGACATGCTTCCTGTAGCACCAGAGCCCAGGTCTGC TGAGAGGCCCCAGTGGGACTCTGTGCTACACAGGACTTCATCCCCTGGCAACACCCTTAGCCTGCTGCAG GCTCCTTGTGCCTTGCCGTTAGATAAATGTAGACAGGGAATTGTGTGCAACGGTGCCCTAGAGACTGAAA ACAAGGCCTCAGAACAGCCTGCAGGGTTTGACACCCTTCAGAAGTACCCCACACCCAATGGGCATTGTGC CAATTGGGAGGCTGGGAGGAGCAAGGACTCACTGAGCCACCAGCTGTCTGCCACAAGCTGTAGCTCTGCT CACTTGTACTCGAGGAACTTGCACCACAAGTGGCTGAATAGCCACTCAGGGAGGCCATCCACTACCAGCA GCCCTGACCAGCCTTCCCGCAGCCACCTGGATGACGATGGCATGCCTGTGTACACGGACACAATCCAACA GCGCCTGCGACAGATAGAGTCTGGTCACCAGCAGGAAGTGGAGACCTTGAAGAAACAAGTCCAGGAGTTG AAGAGTCGCCTGGAGAGCCAGTACCTGACCAGCTCTCTGCGCTTCAATGGAGACTTTGGAGATGAAGTGA TGACTCGTTGGCTACCTGACCACCTGGCTGCCCACTGCTATGCCTGTGACAGTGCCTTCTGGCTCGCCAG TAGGAAGCACCACTGCAGGAATTGTGGGAATGTATTCTGCTCCAGTTGTTGTAACCAGAAGGTTCCGGTT CCTAGCCAGCAGCTCTTTGAACCCAGTCGAGTGTGCAAGTCTTGCTATAGCAGCCTCCATCCTACAAGCT CCAGCATTGACCTTGAACTGGATAAGCCTATTGCTGCCACTTCAAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211725 representing BC081544 Red=Cloning site Green=Tags(s) MDEEMRHSLECIQANQIFPRKQLIREDENLQVPFLELHGESTEYVGRAEEAIIALSNYRLHIKFKESLVN VPLQLIESVECRDIFQLHLTCKDCKVIRCQFPTFEQCQDWLKRLNNAIRPPGKIEDLFSFAYHAWCMEVY ASEKEQHGDLCRPGEHVTSRFKNEVERMGFDMNNAWRISNINEKYKLCGSYPQELIVPAWITDKELESVA GFRSWKRIPAVIYRHQSNGAVIARCGQPEVSWWGWRNADDEHLVQSVARACASDSQSSISKVSTRNSCRD FPNAGDLSDVEFDSSLSNTSGAESLALQPQKLLILDARSYAAAVANRAKGGGCECPEYYPNCEVVFMGMA NIHSIRRSFQSLRLLCTQMPDPGNWLSALESTKWLHHLSVLLKSALLVVHAVDRDQRPVLVHCSDGWDRT PQIVALAKLLLDPYYRTIEGFQVLVEMEWLDFGHKFADRCGHGEDSDDLNERCPVFLQWLDCVHQLQRQF PCSFEFNEAFLVKLVQHTYSCLFGTFLCNNAKERGEKQTQERTCSVWSLLRAGNKAFKNLLYSSQSEAVL YPVCHVRNLMLWSAVYLPCPSPSTPTDDSCAPYPVPGTSPDEPPLSRLPKTRSFDNLTTTCENMVPLASR RSSDPSLNEKWQEHGRSLELSSFASAGEEVPAMDSLRKPSRLLGGAELSVAAGVAEGQMENILQEATKEE SGVEEPTHRGHTEVPEVKEEAPLAKESSMAAEGPVVLYQEPQLDDATLRSHQGPSLSLFSQGIPEHQDGH NVLSSSLQAPLSGEDSQEVPVEQPQVENIAEDRENVAPAVPVDAKVGLGISQSSSLLPSQVPFETRGPHI NNSVHMLLEDKVKSESGPQLHHRPCPASSGRFSGKDMLPVAPEPRSAERPQWDSVLHRTSSPGNTLSLLQ APCALPLDKCRQGIVCNGALETENKASEQPAGFDTLQKYPTPNGHCANWEAGRSKDSLSHQLSATSCSSA HLYSRNLHHKWLNSHSGRPSTTSSPDQPSRSHLDDDGMPVYTDTIQQRLRQIESGHQQEVETLKKQVQEL KSRLESQYLTSSLRFNGDFGDEVMTRWLPDHLAAHCYACDSAFWLASRKHHCRNCGNVFCSSCCNQKVPV PSQQLFEPSRVCKSCYSSLHPTSSSIDLELDKPIAATSN TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC081544
ORF Size	3477 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC081544, AAH81544
RefSeq Size	3994 bp
RefSeq ORF	3479 bp
Locus ID	74302
Gene Summary	Phosphatase that acts on lipids with a phosphoinositol headgroup. Has phosphatase activity towards phosphatidylinositol 3-phosphate and phosphatidylinositol 3,5-bisphosphate. May also dephosphorylate proteins phosphorylated on Ser, Thr, and Tyr residues (By similarity).[UniProtKB/Swiss-Prot Function]

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