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Megf10 (NM_001001979) Mouse Tagged ORF Clone
CAT＃: MG211707
- TrueORF®
Megf10 (tGFP-tagged) - Mouse multiple EGF-like-domains 10 (Megf10)
ORF Plasmid: DDK
"NM_001001979" in other vectors (4)
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CNY 9,220.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 3000002B06Rik; Gm331 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211707 representing NM_001001979
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGATTTCTTCAAGTTCGTGCCTGGGCCTCATCTGCTCACTGCTCTGTCACTGGGTGGGGACAGCAT CCTCCCTGAACCTGGAAGACCCCAACGTATGCAGCCACTGGGAAAGCTACTCGGTGACTGTGCAGGAGTC GTATCCACATCCCTTCGATCAGATCTACTACACAAGCTGCACCGACATCCTGAACTGGTTTAAATGCACA CGGCACAGAATCAGCTACCGGACAGCCTACCGCCACGGGGAGAAAACCATGTATAGACGCAAATCCCAGT GTTGCCCAGGATTTTATGAAAGCCGAGACATGTGTGTCCCTCACTGTGCTGATAAATGTGTCCATGGTCG CTGCATTGCTCCAAACACCTGTCAGTGTGAGCCTGGCTGGGGTGGGACCAACTGTAGCAGTGCTTGTGAT GGTGATCACTGGGGGCCTCACTGCAGCAGCCGATGCCAGTGCAAAAACAGAGCTTTGTGTAACCCCATCA CCGGTGCTTGCCACTGCGCTGCGGGCTACCGGGGATGGCGCTGCGAGGACCGTTGTGAACAGGGCACGTA CGGTAACGACTGTCACCAAAGATGCCAGTGTCAGAATGGAGCGACCTGTGACCACATCACTGGGGAATGC CGTTGTTCACCTGGGTACACTGGAGCCTTCTGTGAGGATCTTTGTCCTCCTGGCAAACATGGTCCACATT GTGAGCAGAGGTGTCCCTGCCAAAATGGGGGTGTGTGCCACCATGTCACTGGAGAGTGCTCTTGCCCTTC TGGTTGGATGGGCACAGTGTGTGGTCAGCCCTGCCCTGAGGGTCGCTTTGGAAAGAACTGTTCCCAAGAA TGCCAGTGTCACAATGGAGGAACGTGTGATGCTGCCACAGGCCAGTGTCACTGCAGCCCAGGATACACAG GGGAACGGTGTCAGGACGAATGTCCTGTTGGGAGCTATGGAGTTCGCTGTGCTGAGGCCTGCAGGTGTGT CAACGGAGGCAAGTGTTACCACGTGAGTGGTACATGCCTGTGCGAAGCAGGCTTTTCGGGTGAACTTTGC GAGGCGCGCCTGTGTCCCGAGGGGCTTTACGGCATCAAATGTGACAAGCGGTGCCCCTGCCACCTGGACA ACACTCACAGCTGTCATCCCATGTCTGGAGAGTGTGGCTGCAAGCCGGGTTGGTCGGGACTGTACTGTAA TGAAACATGCTCCCCTGGATTCTACGGGGAGGCTTGCCAACAGATCTGCAGCTGCCAGAACGGGGCGGAC TGCGACAGTGTGACTGGAAGGTGTGCCTGCGCTCCAGGATTCAAAGGGACTGACTGCTCTACTCCGTGTC CTCTGGGACGCTACGGGATAAATTGTTCTTCTCGCTGTGGCTGTAAAAATGATGCTGTCTGTTCTCCTGT GGATGGATCATGTATCTGTAAGGCAGGCTGGCACGGGGTGGACTGTTCCATCCGCTGCCCCAGTGGCACA TGGGGCTTTGGCTGTAACCTAACGTGTCAGTGCCTCAATGGCGGTGCCTGCAACACGCTGGATGGGACCT GCACCTGTGCGCCCGGATGGCGAGGCGCGAAGTGTGAATTTCCCTGCCAGGATGGCACTTATGGGCTGAA CTGTGCCGAGCGCTGTGACTGCAGCCATGCAGATGGCTGTCACCCCACTACAGGCCATTGCCGCTGCCTC CCTGGATGGTCAGGTGTGCACTGTGACAGTGTGTGCGCTGAGGGACGCTGGGGTCCTAACTGCTCGCTGC CCTGCTACTGTAAAAATGGGGCTTCGTGTTCTCCGGATGATGGCATCTGTGAGTGTGCACCCGGATTCCG AGGCACCACTTGCCAGAGAATCTGCTCCCCCGGTTTTTATGGACATCGCTGTAGCCAGACCTGCCCGCAG TGTGTGCACAGCAGTGGGCCCTGCCACCACATCACGGGCCTGTGTGACTGCTTACCTGGCTTCACCGGTG CCCTGTGCAATGAAGTGTGTCCCAGTGGCAGATTTGGGAAAAACTGTGCAGGCGTTTGTACTTGCACCAA CAATGGCACCTGTAACCCCATCGACAGATCCTGCCAGTGTTACCCAGGCTGGATTGGCAGTGACTGCTCC CAGCCCTGTCCACCTGCGCACTGGGGTCCGAACTGCATCCACACCTGCAACTGCCACAACGGAGCCTTTT GCAGCGCCTATGATGGGGAATGCAAATGCACTCCTGGCTGGACGGGGCTCTACTGCACTCAGAGATGCCC TCTGGGCTTCTATGGTAAGGACTGTGCACTGATATGCCAATGTCAAAACGGAGCTGACTGCGACCATATC TCGGGGCAGTGTACCTGCCGCACGGGATTCATGGGACGGCACTGTGAACAGAAGTGCCCTGCGGGAACAT ACGGCTATGGCTGTCGCCAGATCTGTGACTGTCTGAACAACTCCACCTGTGACCACATCACTGGCACGTG TTACTGTAGCCCAGGATGGAAAGGGGCACGATGTGACCAAGCTGGGGTTATCATCGTGGGCAATCTGAAC AGCTTAAGCCGGACCAGCACCGCCCTTCCTGCCGATTCCTATCAGATCGGGGCCATCGCGGGCATCGTGG TCCTCGTTCTTGTTGTGCTCTTCCTGCTGGCGCTGTTCATCATCTACAGACACAAGCAGAAGAGGAAGGA ATCAAGCATGCCGGCCGTGACCTACACCCCCGCCATGAGGGTCATCAATGCAGACTATACCATCGCAGAA ACCCTGCCTCACAGCAATGGTGGAAATGCCAACAGCCACTACTTTACCAATCCCAGTTATCACACACTTA GCCAGTGTGCCACATCCCCTCATGTGAACAATAGGGACAGGATGACCATTGCAAAGTCAAAAAACAATCA GCTGTTTGTGAATCTTAAAAATGTGAATCCAGGGAAGAGAGGGACATTGGTGGACTGCACTGGGACATTG CCAGCTGACTGGAAGCAAGGAGGCTACCTCAATGAGCTTGGTGCTTTCGGGCTGGACAGAAGCTACATGG GAAAGTCCTTAAAAGATCTGGGGAAGAACTCTGAATATAATTCAAGCACTTGCTCCTTAAGCAGCTCTGA AAACCCATATGCCACCATTAAAGACCCGCCTGCACTCCTGCCTAAAAGCTCCGAGTGCGGCTACGTGGAG ATGAAGTCGCCGGCGCGGAGAGACTCCCCATATGCAGAGATCAACAACTCAACTCCAGCCAACAGGAATG TCTATGAAGTCGAACCTACAGTGAGCGTTGTGCAAGGAGTATTCAGCAACAGCGGTCACGTCACCCAAGA CCCATATGACCTTCCAAAGAACAGTCACATCCCTTGCCATTATGACCTGCTGCCAGTAAGGGACAGTTCA TCCTCCCCAAAGAGAGAGGATGGTGGTGGCAGCAACAGCACCAGCAGCAACAGCACCAGCAGCAGCAGCA GCAGCAGTGAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211707 representing NM_001001979
Red=Cloning site Green=Tags(s) MAISSSSCLGLICSLLCHWVGTASSLNLEDPNVCSHWESYSVTVQESYPHPFDQIYYTSCTDILNWFKCT RHRISYRTAYRHGEKTMYRRKSQCCPGFYESRDMCVPHCADKCVHGRCIAPNTCQCEPGWGGTNCSSACD GDHWGPHCSSRCQCKNRALCNPITGACHCAAGYRGWRCEDRCEQGTYGNDCHQRCQCQNGATCDHITGEC RCSPGYTGAFCEDLCPPGKHGPHCEQRCPCQNGGVCHHVTGECSCPSGWMGTVCGQPCPEGRFGKNCSQE CQCHNGGTCDAATGQCHCSPGYTGERCQDECPVGSYGVRCAEACRCVNGGKCYHVSGTCLCEAGFSGELC EARLCPEGLYGIKCDKRCPCHLDNTHSCHPMSGECGCKPGWSGLYCNETCSPGFYGEACQQICSCQNGAD CDSVTGRCACAPGFKGTDCSTPCPLGRYGINCSSRCGCKNDAVCSPVDGSCICKAGWHGVDCSIRCPSGT WGFGCNLTCQCLNGGACNTLDGTCTCAPGWRGAKCEFPCQDGTYGLNCAERCDCSHADGCHPTTGHCRCL PGWSGVHCDSVCAEGRWGPNCSLPCYCKNGASCSPDDGICECAPGFRGTTCQRICSPGFYGHRCSQTCPQ CVHSSGPCHHITGLCDCLPGFTGALCNEVCPSGRFGKNCAGVCTCTNNGTCNPIDRSCQCYPGWIGSDCS QPCPPAHWGPNCIHTCNCHNGAFCSAYDGECKCTPGWTGLYCTQRCPLGFYGKDCALICQCQNGADCDHI SGQCTCRTGFMGRHCEQKCPAGTYGYGCRQICDCLNNSTCDHITGTCYCSPGWKGARCDQAGVIIVGNLN SLSRTSTALPADSYQIGAIAGIVVLVLVVLFLLALFIIYRHKQKRKESSMPAVTYTPAMRVINADYTIAE TLPHSNGGNANSHYFTNPSYHTLSQCATSPHVNNRDRMTIAKSKNNQLFVNLKNVNPGKRGTLVDCTGTL PADWKQGGYLNELGAFGLDRSYMGKSLKDLGKNSEYNSSTCSLSSSENPYATIKDPPALLPKSSECGYVE MKSPARRDSPYAEINNSTPANRNVYEVEPTVSVVQGVFSNSGHVTQDPYDLPKNSHIPCHYDLLPVRDSS SSPKREDGGGSNSTSSNSTSSSSSSSE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001001979 |
| ORF Size | 3441 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001001979.2, NP_001001979.1 |
| RefSeq Size | 7514 bp |
| RefSeq ORF | 3444 bp |
| Locus ID | 70417 |
| UniProt ID | Q6DIB5 |
| Gene Summary | Membrane receptor involved in phagocytosis by macrophages and astrocytes of apoptotic cells. Receptor for C1q, an eat-me signal, that binds phosphatidylserine expressed on the surface of apoptotic cells (PubMed:27170117). Cooperates with ABCA1 within the process of engulfment (By similarity). Promotes the formation of large intracellular vacuoles and may be responsible for the uptake of amyloid-beta peptides (PubMed:20828568). Necessary for astrocyte-dependent apoptotic neuron clearance in the developing cerebellum (PubMed:27170117). Plays role in muscle cell proliferation, adhesion and motility. Is also an essential factor in the regulation of myogenesis. Controls the balance between skeletal muscle satellite cells proliferation and differentiation through regulation of the notch signaling pathway (PubMed:28498977). May also function in the mosaic spacing of specific neuron subtypes in the retina through homotypic retinal neuron repulsion. Mosaics provide a mechanism to distribute each cell type evenly across the retina, ensuring that all parts of the visual field have access to a full set of processing elements (PubMed:22407321).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223726 | Megf10 (untagged) - Mouse multiple EGF-like-domains 10 (Megf10), (10ug) |
CNY 14,820.00 |
|
| MR211707 | Megf10 (Myc-DDK-tagged) - Mouse multiple EGF-like-domains 10 (Megf10) |
CNY 7,992.00 |
|
| MR211707L3 | Lenti ORF clone of Megf10 (Myc-DDK-tagged) - Mouse multiple EGF-like-domains 10 (Megf10) |
CNY 10,260.00 |
|
| MR211707L4 | Lenti ORF clone of Megf10 (mGFP-tagged) - Mouse multiple EGF-like-domains 10 (Megf10) |
CNY 10,260.00 |
