Sema5b (BC052397) Mouse Tagged ORF Clone

CAT＃: MG211660

  • TrueORF®

Sema5b (tGFP-tagged) - Mouse sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic

ORF Plasmid: DDK tGFP

Datasheet
SDS
Plasmid Map

  "BC052397" in other vectors (3)

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Mouse Protein / Lysate Request

CNY 12,350.00

货期*
5周
规格
    • 10 ug

Product images

经常一起买 (4)
PS100010
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00

添加到购物车
TA150041
Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00

添加到购物车

【特别福利】购一抗正装产品（≥50ul或≥50ug），免费领10ul抗体试用装

CC100003
DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00

暂停销售
PRIMER
Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

添加到购物车

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms SemG, mKIAA1445
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211660 representing BC052397
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTAGTCCCAGGACCCCTGGCTCTCTCGCTGTTGCTGTCCAGCCTCACCCTGCTGGTGTCCCACCTCT
CCAGCTCCCAGGACATTGCCAGTGAGTCCAGCAGTGAGCAACAGATGTGCACGCGGAGGGAGCACCCCAT
CGTGGCCTTTGAAGATCTGAAGCCGTGGGTCTTTAACTTCACCTACCCTGGAGTCCGGGATTTCTCCCAG
CTTGCTCTTGATCCCTCGAGGAATCAGCTCATCGTGGGAGCCAGGAACTACCTCTTCAGACTCAGCCTCG
CCAATGTCTCCCTCCTTCAGGCTACAGAGTGGGCCTCCAGTGAGGACACGCGCCGCTCCTGCCAGAGCAA
AGGGAAAACGGAGGAGGAGTGTCAGAACTATGTACGAGTCCTGATTGTTTCCGGCCGGAAGGTGTTCATG
TGCGGTACCAATGCCTTTTCCCCAGTGTGCTCCAGCAGACAGGTAGGGAACCTCAGCCGGACTATTGAGA
AGATCAACGGTGTGGCCCGGTGCCCCTATGACCCACGCCACAACTCTACAGCCGTCATCTCCTCTCAGGG
GGAGCTCTATGCAGCCACAGTCATTGACTTCTCCGGTCGGGACCCAGCCATCTACCGCAGCCTGGGCAGT
GGGCCACCGCTTCGCACTGCCCAGTATAACTCCAAATGGCTCAATGAGCCAAATTTTGTGGCAGCCTTTG
ACATCGGGCTGTTTGCGTATTTCTTCCTTCGGGAGAATGCCGTGGAGCATGATTGTGGGCGCACTGTGTA
CTCTCGGGTGGCCCGGGTGTGCAAGAATGATGTAGGTGGCCGTTTCCTGCTGGAGGACACGTGGACCACA
TTCATGAAGGCCCGGCTCAACTGCTCCCGCCCGGGAGAGGTCCCCTTCTACTATAATGAGCTGCAGAGTG
CCTTCCATCTGCCCGAGCAGGACCTCATCTATGGCGTCTTCACCACTAACGTAAACAGCATTGCGGCTTC
TGCTGTCTGCGCCTTCAACCTCAGTGCCATCTCCAAGGCTTTCAATGGCCCATTTCGTTACCAGGAAAAC
CCCAGGGCTGCCTGGCTCCCAATCGCCAATCCCATCCCCAATTTCCAGTGTGGCACTCTGCCGGAGACTG
GCCCCAACGAGAACCTCACGGAGCGCAGCCTGCAGGACGCACAGCGGCTCTTCCTGATGAGCGAAGCTGT
GCAGCCAGTGACACCAGAGCCCTGTGTCACCCAGGACAGCGTCCGCTTCTCACATCTCGTGGTAGACCTT
GTGCAAGCTAAGGACACGCTCTATCACGTACTCTACATAGGCACGGAGTCGGGTACCATCCTGAAAGCGC
TGTCCACGGCCAGCCGCAGCCTCCGTGGCTGCTACCTGGAGGAGCTACATGTGCTGCCTCCTGGGCGCCT
TGAACCTCTGCGGAGCCTGCGCATCCTGCACAGCGCGCGTGCGCTCTTCGTGGGATTGAGCGACAGGGTG
CTGCGGGTCCCACTGGAGAGGTGTTCGGCCTATCATAGCCAGGGGGCATGCCTGGGAGCACGGGACCCAT
ACTGCGGCTGGGATGGGAAGCGGCAACTTTGCAGCACGCTTGAAGACAGTTCCAACATGAGCCTGTGGAT
CCAGAACATCACAACCTGTCCTGTACGAAATGTGACACGGGATGGGGGCTTCGGCCCATGGTCACCATGG
AAACCGTGTGAGCACTTAGATGGAGACAACTCGGGTTCTTGCCTGTGCCGGGCCAGATCCTGTGACTCCC
CAAGGCCTCGCTGTGGGGGCCTCGAGTGCCTGGGGCCATCCATCCATATTGCCAATTGCTCCAGGAATGG
GGCGTGGACCGCATGGTCATCGTGGGCTCAGTGCAGCACGTCCTGTGGGATCGGCTTCCAGGTCCGTCAG
CGAAGTTGCAGCAACCCGGCGCCCCGCCATGGGGGCCGCATTTGCGTGGGCAAGAGCCGGGAGGAGCGGT
TCTGTAATGAAAATACACCTTGCCCAGTGCCCATCTTCTGGGCTTCCTGGGGTTCCTGGAGCAAGTGCAG
CAACAACTGTGGAGGCGGCGTGCAGTCGCGACGCCGTTCTTGCGAGAATGGCAACTCGTGCCCGGGTTGC
GGCGTGGTGAGGTCGCGGGGCTGGGGCGCGGGGAGCGGGGAGACCTACAGTCCAGGCGGGGTTGGGGGGT
CTGAAGCCACCCTCACCCCGAAGGAGTTCAAGACCTGCAACCCCGAGGCTTGCCCGGAAGTGCGACGCAA
CACACCCTGGACGCCCTGGCTGCCCGTGAACGTGACCCAGGGTGGAGCGCGCCAGGAGCAGCGATTCCGC
TTCACCTGCCGCGCGCCGCTGCCGGACCCGCACGGTCTGCAGTTCGGCAAGAGGAGGACAGAGACCAGGA
CTTGCCCGGCAGACGGCACCGGAGCCTGCGACACCGACGCCCTGGTGGAGGATCTCCTGCGCAGCGGGAG
CACGTCACCACACACTCTGAACGGAGGCTGGGCCACCTGGGGCCCGTGGTCATCCTGCTCCCGGGACTGC
GAGCTGGGCTTCCGCGTCCGCAAGAGAACTTGTACCAACCCGGAGCCTCGCAACGGGGGCTTGCCCTGCG
TGGGAGACGCTGCGGAGTACCAAGACTGCAACCCACAGGCTTGCCCAGTGCGGGGTGCTTGGTCCTGCTG
GACCGCATGGTCCCAGTGCTCAGCATCCTGCGGTGGTGGCCACTATCAACGCACCCGTTCCTGCACCAGC
CCCGCCCCATCCCCAGGTGAGGACATCTGCCTCGGCCTGCACACGGAGGAGGCCCTATGTTCAACACAGG
CCTGCCCAGAAGGCTGGTCACTGTGGTCTGAGTGGGGTGTCTGCACTGAGGATGGGGCCCAGAGCCGGAG
CCGGAGCTGTGAGGAGCTTCTCCCAGGACCAGGTGCCTGTGTTGGCAACAGCAGCCAGAGCCGGCCCTGC
CCCTACAGTGAGATTCCTGTCATCCTACCTGCTTCCAGTGTGGAGGAGACCACCAGCTGTGGAGGGTTCA
ATCTCATCCACCTGATAGTCACTGGTGTGTCCTGCTTCCTGGTTTCTGGGCTCTTGACCTTGGCAGTGTA
CCTGTCTTGCCAGCACTGCCAGCGCCAGTCTCAGGAGTCCACGCTTGTCCATCCTGCCACACCTAACCAC
TTGCACTACAAGGGTGGGGGCACCCCCAAGAATGAGAAGTACACCCCTATGGAATTCAAGACACTGAACA
AGAACAACTTAATCCCTGATGACAGAGCCAACTTCTACCCACTGCAGCAGACCAATGTGTATACAACCAC
GTACTACCCCAGCCCACTGAACAAGCCCAGCTTCCGGCCTGAGGCCTCACCTGGACAGCGCTGTTTCCCC
AACAGC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211660 representing BC052397
Red=Cloning site Green=Tags(s)

MVVPGPLALSLLLSSLTLLVSHLSSSQDIASESSSEQQMCTRREHPIVAFEDLKPWVFNFTYPGVRDFSQ
LALDPSRNQLIVGARNYLFRLSLANVSLLQATEWASSEDTRRSCQSKGKTEEECQNYVRVLIVSGRKVFM
CGTNAFSPVCSSRQVGNLSRTIEKINGVARCPYDPRHNSTAVISSQGELYAATVIDFSGRDPAIYRSLGS
GPPLRTAQYNSKWLNEPNFVAAFDIGLFAYFFLRENAVEHDCGRTVYSRVARVCKNDVGGRFLLEDTWTT
FMKARLNCSRPGEVPFYYNELQSAFHLPEQDLIYGVFTTNVNSIAASAVCAFNLSAISKAFNGPFRYQEN
PRAAWLPIANPIPNFQCGTLPETGPNENLTERSLQDAQRLFLMSEAVQPVTPEPCVTQDSVRFSHLVVDL
VQAKDTLYHVLYIGTESGTILKALSTASRSLRGCYLEELHVLPPGRLEPLRSLRILHSARALFVGLSDRV
LRVPLERCSAYHSQGACLGARDPYCGWDGKRQLCSTLEDSSNMSLWIQNITTCPVRNVTRDGGFGPWSPW
KPCEHLDGDNSGSCLCRARSCDSPRPRCGGLECLGPSIHIANCSRNGAWTAWSSWAQCSTSCGIGFQVRQ
RSCSNPAPRHGGRICVGKSREERFCNENTPCPVPIFWASWGSWSKCSNNCGGGVQSRRRSCENGNSCPGC
GVVRSRGWGAGSGETYSPGGVGGSEATLTPKEFKTCNPEACPEVRRNTPWTPWLPVNVTQGGARQEQRFR
FTCRAPLPDPHGLQFGKRRTETRTCPADGTGACDTDALVEDLLRSGSTSPHTLNGGWATWGPWSSCSRDC
ELGFRVRKRTCTNPEPRNGGLPCVGDAAEYQDCNPQACPVRGAWSCWTAWSQCSASCGGGHYQRTRSCTS
PAPSPGEDICLGLHTEEALCSTQACPEGWSLWSEWGVCTEDGAQSRSRSCEELLPGPGACVGNSSQSRPC
PYSEIPVILPASSVEETTSCGGFNLIHLIVTGVSCFLVSGLLTLAVYLSCQHCQRQSQESTLVHPATPNH
LHYKGGGTPKNEKYTPMEFKTLNKNNLIPDDRANFYPLQQTNVYTTTYYPSPLNKPSFRPEASPGQRCFP
NS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC052397
ORF Size 3366 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC052397.1
RefSeq Size 4658 bp
RefSeq ORF 3368 bp
Locus ID 20357
Gene Summary May act as positive axonal guidance cues.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

SKU Description Size Price
MR211660 Sema5b (Myc-DDK-tagged) - Mouse sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic
    • 10 ug

CNY 11,210.00

MR211660L3 Lenti ORF clone of Sema5b (Myc-DDK-tagged) - Mouse sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic
    • 10 ug

CNY 13,110.00

MR211660L4 Lenti ORF clone of Sema5b (mGFP-tagged) - Mouse sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic
    • 10 ug

CNY 13,110.00

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MR211660L4

Lenti ORF clone of Sema5b (mGFP-tagged) - Mouse sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic

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CNY 13,110.00

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CNY 13,110.00

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