Nlrp5 (BC053384) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	Mater; Nalp5; Op1; PAN11
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211648 representing BC053384 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGTCCTCCAGAAAAAGAAAGTAAAGCAATCTTGAAAGCACGTGGATTGGAAGAGGAACAGAAGTCAG AAAGAAAAATGACTTCTCCAGAAAACGACAGTAAATCAATCCAGAAAGACCAAGGACCAGAGCAGGAGCA GACATCAGAAAGCACAATGGGTCCTCCAGAAAAAGACAGTAAAGCAATCTTGAAAGCACGTGGATTGGAA GAGGAACAGAAGTCAGAAAGCACAATGTCTCCTTCAGAAAATGTCAGTAGAGCAATCCTGAAAGACAGTG GATCAGAAGAAGTGGAACAGGCGTCAGAAAGAAAAATGACTTCTCCAGAAAACGACAGTAAATCAATCCA GAAAGACCAAGGACCAGAGCAGGAGCAGACATCAGAAACCTTACAATCTAAGGAAGAAGATGAAGTGACA GAGGCAGATAAAGATAATGGAGGTGACTTACAAGACTACAAGGCCCATGTGATTGCTAAGTTCGACACAA GTGTGGATCTACACTATGACAGCCCAGAGATGAAATTATTGTCTGATGCTTTTAAACCATACCAGAAAAC CTTCCAGCCTCACACCATTATCCTACATGGAAGACCAGGAGTTGGGAAGTCAGCTTTGGCCAGAAGTATT GTTCTTGGCTGGGCACAGGGTAAACTCTTCCAAAAAATGTCCTTTGTCATCTTCTTCTCTGTTAGAGAAA TAAAGTGGACAGAGAAGAGCAGTTTGGCACAGCTGATTGCTAAGGAGTGTCCAGACTCCTGGGATCTAGT GACAAAGATCATGTCCCAACCAGAAAGACTCTTGTTTGTCATAGATGGCTTGGATGATATGGACTCTGTC CTCCAACATGATGATATGACACTATCCAGAGACTGGAAGGATGAACAGCCCATATACATCCTGATGTACA GCCTCCTGAGGAAGGCTCTCCTACCTCAGTCCTTTCTCATCATTACCACCAGAAACACAGGCTTAGAAAA ACTCAAGTCAATGGTTGTGTCCCCCCTCTATATACTGGTTGAAGGACTGTCTGCATCAAGGAGATCTCAG CTGGTCCTCGAGAACATCTCCAATGAGTCTGATAGAATACAAGTCTTCCATTCTCTGATAGAAAATCACC AGCTGTTTGACCAATGCCAGGCCCCCTCTGTGTGCTCCCTGGTCTGTGAGGCTCTACAGCTACAGAAGAA ACTGGGAAAGAGATGCACCCTACCCTGCCAGACTCTCACCGGTTTGTATGCCACGTTGGTGTTTCACCAG CTCACCTTGAAAAGGCCTTCCCAGAGCGCTCTCAGTCAGGAAGAACAGATTACTCTAGTGGGTTTGTGCA TGATGGCAGCTGAAGGAGTGTGGACCATGAGGTCGGTATTCTATGATGATGACCTGAAGAACTATAGCCT AAAGGAGTCTGAGATCTTGGCCCTCTTTCACATGAACATCCTTCTCCAGGTTGGCCACAACAGTGAGCAG TGTTATGTTTTCTCCCACCTCAGCCTGCAGGATTTCTTTGCTGCCTTATATTATGTTTTAGAAGGGCTGG AGGGATGGAATCAGCATTTTTGCTTCATTGAAAACCAAAGGAGCATCATGGAGGTGAAGAGAACTGACGA CACTCGCCTCCTCGGGATGAAGCGTTTCTTATTTGGCCTCATGAACAAGGATATCTTGAAGACTCTGGAG GTTCTGTTTGAATATCCCGTGATTCCAACTGTTGAGCAGAAGCTCCAACACTGGGTCTCTCTGATAGCTC AGCAGGTCAATGGCACCAGCCCAATGGACACCCTGGATGCCTTCTATTGTCTATTTGAGTCTCAGGATGA AGAGTTTGTTGGCGGGGCTCTCAAACGCTTCCAAGAAGTGTGGCTGCTGATTAACCAGAAGATGGACTTG AAGGTCTCTTCCTACTGTCTCAAGCACTGTCAGAACTTGAAGGCAATCCGGGTGGATATCAGAGACCTCC TCTCGGTAGATAATACTCTCGAGCTGTGCCCTGTTGTTACTGTCCAGGAGACACAATGTAAGCCCCTCCT CATGGAGTGGTGGGGAAACTTCTGCTCTGTGCTTGGCAGCCTCCGGAACTTGAAGGAGCTGGACTTGGGC GACAGCATCCTGAGTCAACGGGCCATGAAGATACTGTGCCTCGAGCTGCGGAATCAGTCCTGCAGAATAC AGAAGCTGACGTTTAAGAGTGCAGAGGTAGTGTCTGGCCTGAAACATCTCTGGAAGCTCCTTTTTAGCAA TCAAAACTTAAAGTACCTCAATCTAGGGAACACTCCCATGAAGGATGATGACATGAAGTTAGCCTGCGAA GCGCTGAAACATCCAAAGTGCTCCGTGGAGACTCTGAGGTTGGATTCCTGTGAGTTAACCATCATTGGTT ATGAGATGATCTCCACGCTTCTTATTTCAACCACCAGGCTAAAGTGTCTCAGCCTGGCCAAAAATAGAGT GGGAGTAAAAAGCATGATATCCCTTGGGAATGCCTTGAGTAGCTCAATGTGTCTACTGCAAAAGTTGATA CTGGACAACTGTGGCCTCACACCTGCCAGCTGCCACCTTCTGGTCTCAGCCCTTTTCAGCAACCAGAACT TGACACACCTGTGCCTGTCAAACAACAGCCTGGGGACTGAAGGAGTGCAACAGCTGTGTCAGTTCCTGAG GAATCCAGAATGTGCTCTCCAGCGGCTGATACTGAATCACTGCAACATTGTAGATGATGCTTATGGCTTC CTGGCAATGAGACTTGCAAACAACACAAAGCTGACCCACCTGAGCCTGACCATGAACCCCGTAGGGGATG GTGCAATGAAGCTACTGTGTGAAGCTTTAAAGGAACCTACTTGTTACCTTCAAGAACTGGAACTAGTGGA CTGCCAACTCACACAGAACTGCTGCGAGGACCTGGCCTGTATGATCACAACAACCAAGCACTTAAAAAGT TTGGATCTTGGTAACAACGCCCTGGGTGACAAAGGAGTCATAACCCTGTGTGAGGGACTGAAGCAAAGTA GCAGCTCCCTGAGGAGACTTGGGTTGGGGGCATGTAAGTTGACTTCCAATTGCTGTGAGGCATTGTCATT GGCCATCTCTTGCAACCCTCACCTGAACAGCCTAAACCTGGTGAAGAATGACTTCAGTACATCGGGGATG TTGAAGCTGTGCTCTGCGTTCCAATGCCCTGTCTCTAACCTGGGGATAATTGGCCTGTGGAAGCAGGAGT ACTATGCCCGAGTGAGAAGACAGCTGGAGGAAGTTGAGTTTGTCAAGCCCCACGTGGTGATTGATGGTGA TTGGTATGCTAGTGATGAAGATGACCGAAACTGGTGGAAAAAC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211648 representing BC053384 Red=Cloning site Green=Tags(s) MGPPEKESKAILKARGLEEEQKSERKMTSPENDSKSIQKDQGPEQEQTSESTMGPPEKDSKAILKARGLE EEQKSESTMSPSENVSRAILKDSGSEEVEQASERKMTSPENDSKSIQKDQGPEQEQTSETLQSKEEDEVT EADKDNGGDLQDYKAHVIAKFDTSVDLHYDSPEMKLLSDAFKPYQKTFQPHTIILHGRPGVGKSALARSI VLGWAQGKLFQKMSFVIFFSVREIKWTEKSSLAQLIAKECPDSWDLVTKIMSQPERLLFVIDGLDDMDSV LQHDDMTLSRDWKDEQPIYILMYSLLRKALLPQSFLIITTRNTGLEKLKSMVVSPLYILVEGLSASRRSQ LVLENISNESDRIQVFHSLIENHQLFDQCQAPSVCSLVCEALQLQKKLGKRCTLPCQTLTGLYATLVFHQ LTLKRPSQSALSQEEQITLVGLCMMAAEGVWTMRSVFYDDDLKNYSLKESEILALFHMNILLQVGHNSEQ CYVFSHLSLQDFFAALYYVLEGLEGWNQHFCFIENQRSIMEVKRTDDTRLLGMKRFLFGLMNKDILKTLE VLFEYPVIPTVEQKLQHWVSLIAQQVNGTSPMDTLDAFYCLFESQDEEFVGGALKRFQEVWLLINQKMDL KVSSYCLKHCQNLKAIRVDIRDLLSVDNTLELCPVVTVQETQCKPLLMEWWGNFCSVLGSLRNLKELDLG DSILSQRAMKILCLELRNQSCRIQKLTFKSAEVVSGLKHLWKLLFSNQNLKYLNLGNTPMKDDDMKLACE ALKHPKCSVETLRLDSCELTIIGYEMISTLLISTTRLKCLSLAKNRVGVKSMISLGNALSSSMCLLQKLI LDNCGLTPASCHLLVSALFSNQNLTHLCLSNNSLGTEGVQQLCQFLRNPECALQRLILNHCNIVDDAYGF LAMRLANNTKLTHLSLTMNPVGDGAMKLLCEALKEPTCYLQELELVDCQLTQNCCEDLACMITTTKHLKS LDLGNNALGDKGVITLCEGLKQSSSSLRRLGLGACKLTSNCCEALSLAISCNPHLNSLNLVKNDFSTSGM LKLCSAFQCPVSNLGIIGLWKQEYYARVRRQLEEVEFVKPHVVIDGDWYASDEDDRNWWKN TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC053384
ORF Size	3333 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC053384, AAH53384
RefSeq Size	3534 bp
RefSeq ORF	3335 bp
Locus ID	23968
Gene Summary	This gene encodes a member of the NACHT, leucine-rich repeat, and pyrin domain containing family. Members of this family have a pyrin domain at the N-terminus, a central NACHT domain, and a C-terminal leucine-rich repeat domain. This gene encodes a maternal-effect factor that is essential for early embryonic development in the mouse. Homozygous null mutant females are sterile, and embryos die following the first cleavage. This gene is required for endoplasmic reticulum redistribution and calcium homeostasis in oocytes. In addition, ovulated oocytes mutant for this gene have abnormal mitochondrial localization and increased mitochondrial activity, which results in mitochondrial damage and early embryonic lethality. Pseudogenes of this gene have been found on chromosomes 7 and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

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