Pcdh8 (BC053008) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	1700080P15Rik; Papc
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211582 representing BC053008 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTCCTGCGAAGCGCTGGGGCAGCCCTTGCCTTTTCCCCTTGCAGCTATTTAGTCTCTGCTGGGTGC TCTCAGTGGCCCAGAGCAAGACAGTCCGATACAGCACGTTCGAAGAGGATGCCCCTGGCACAGTCATCGG AACCCTTGCAGAAGACCTGCACATGAAAGTGTCTGGAGACACAAGCTTCCGCCTGATGAAGCAATTCAAC AGCTCTCTGCTCCGGGTACGCGAGGGCGACGGGCAGCTGACCGTCGGGGATGCGGGCCTGGACCGTGAGC GTCTGTGTGGCCCGTCCCCACAGTGCGTGCTGGCCTTCGATGTTGTCAGCTTCTCGCAGGAACAGTTCCG CCTGGTGCACGTGGAGGTGGAGGTGAGGGATGTAAACGACCACGCGCCCCGCTTTCCCCGAGCCCAGATC CCGGTGGAGGTCTCAGAGAGCGCTCCGGTAGGCACACGCATCCCCCTGGAGGTGCCCGTGGACGAGGACG TGGGTGCCAACGGGCTGCAGAGTGTACGCCTAGCCGAGCCTCACAGCCCCTTCCGCGTGGAGCTGCAGAC GCGCGCGGACGGTGCCCAGTGTGCGGACCTGGTGCTGCTACAGGAGTTGGACCGCGAGAGCCAGGCCTCC TACAGCCTGGAGCTGGTGGCTCAGGATGGCGGGCGACCGCCGCGTTCCGCCACAGCAGCCCTCAGCGTGC GTGTGCTAGATGCCAATGACCACAGCCCGGCCTTCCCACAGGGTGCCGTGGCAGAGGTGGAATTGGCCGA GGACGCACCTGTGGGATCGCTGCTGCTCGACCTGGACGCTGCCGATCCCGACGAAGGTCCCAACGGCGAC GTTGTGTTCACCTTCGGCGCCCGCACCCCTCCCGAAGCCCGCCACCTCTTCCGGCTCGACCCGCGCTCTG GCCGCCTCACCTTGGCTGGGCAGGTGGACTACGAGCGCCAGGACACCTACGAGCTGGACGTGCGGGCTCA AGACCGAGGTCCAGGGCCCCGGACAGCCACCTGCAAGGTTATCGTGCGCATCCGAGACGTCAACGACAAC GCTCCTGAAATTTCTATCACTCCTCTGGCCGCCCCGGGGGCTCCAGCCACCTCGCCCTTCGCCGCCGCCG CCGCTGCCGCCGCACTCGGAGGAGCGGACGCGGCCTCGTCGACTGGGTCCGGGACACAGGAAGCCGGTAT CACCTCGCTGGTGCCAGAGGGGGCGGCGCGCGAGAGTCTAGTGGCGCTGGTCAGCACCTCGGACAGGGAC TCGGGCGCCAACGGGCAGGTGCGCTGCGCCCTCTACGGGCACGAGCACTTTAGGCTGCAGCCGGCCTATG CTGGCAGCTACCTGGTGGTGACCGCTGCATCTCTGGACCGGGAGCGCATCGCGGAGTACAACCTGACGCT GGTGGCCGAAGACCGTGGCACACCCCCTCTTCGCACCGTCAGGCCCTACACCGTGCGAGTGGGGGACGAG AACGACAACGCACCGATCTTCACCAAGCCAGTCTATGAGGTGTCGGTCCGCGAAAACAACCCTCCAGGCG CCTACCTGGCCACAGTGGCAGCCCGCGACCCTGACGTGGGTCGCAACGGTCAAGTCACCTACCGCCTGGT AGAGGCCGAAGTGGGCCGCTCTGGGGAAGCCGTGTCCACTTACGTCTCAGTAGACCCGGCTACCGGAGCC ATCTACGCTCTCCGTAGTTTTGACTATGAGACTCTGCGCCAGCTTGACGTGCGTGTCCAGGCCAGCGACG GCGGTTCCCCTCAGCTTTCCAGCAATGCTCTGGTGCAAGTGCGGGTGCTGGACCAGAATGATCATTCTCC GATCCTTGTGCATCCGGCGCCGGCTAATGGCTCCCTAGAAGTAGCGGTCCCTGGACGTTCCACCAAGGAC ACAGCTGTGGCGCGCATCCAGGCCCGGGATGCTGACGAAGGAGCCAACGGGGAACTGGCCTTTGATCTGC TGCAGCAGGAGCCACGCGAGGCCTTCTCCATAGGCCGCCACACGGGGGAGATCATGCTTACTGGAGACCT CTCGCAGGAGCCTCCTGGCCGCGTGTTCAAGGCCCTGCTGGTCATATCCGACGGCGGCCGTCCCCCTCTC ACCACCACTGCAACCGTCAGTTTCGTGGTAACAGCCGGGGGCGGGTCAGCTGTGCCTGCCAGCTCCGGGA GCCCAGAGCACTCCCGGCCACCCGGCTCTCGCCTCGCGCCGTCGGGGCCTTCGCTGCAGTGGGACACGCC GCTGATCGTCATCATCGTGTTAGCCGGGAGCTGCACGCTGCTGCTTGCAGCCATCATCGCCATCGCCACC ACCTGCAACCGCCGCAAGAAGGAGGTGCGCAAAGGGGGGGCCCTCCGGGAAGAGCGGCCCGGGGCGGCGG GCGGCGGAGCCTCGGCCCCTGGCTCCCCGGATGAAACCGCCAGGGGAACTGGGCCCAGGCCCAACATGTT CGACGTGCTCACCTTCCCTGGCAGCGGCAAAGCGCCCTTTGGCAGCCCCGCGGCCGACGCGCCCCCGCCC GCGGTCGCCGCGGCCGAAGTGCCGGGCTCGGAGGGCGGCAGCGCCACTGGGGAAAGCGCCTGTCACTTCG AGGGGCAGCAGCGGCTCCGAGGCGCGCACGCCGAGCCTTACGGTGCCTCTCCAGGCTTCGGGAAGGAGCC TGCTGCGCCCCCTGTTGCAGTCTGGAAGGGTCATTCATTCAACACCATCTCGGGCCGAGAAGCTGAGAAG TTCAGTGGGAAAGACAGCGGCAAAGGAGACAGTGATTTCAATGACAGTGACTCGGACATCAGCGGGGACG CTTTGAAAAAAGACCTCATCAACCACATGCAGAGTGGACTGTGGGCGTGTACTGCTGAGTGCAAGATCCT AGGTCATTCTGATCGCTGCTGGAGCCCATCCTGTGCCGGACCCAACGTACACCCCCCTCCTCACCCACCA GCCCAGATGTCAACCTTCTGTAAGAGCACGTCCCTGCCTCGGGATCCTCTGCGCAGGGACAATTACTATC AGGCCCAGCTCCCCAAGACAGTAGGGTTGCAGAGCGTCTATGAGAAAGTACTGCATAGAGACTATGACAG GACAGTCACTTTACTCTCCCCTCCCCGTCCAGGAAGGCTCCCAGACCTGCAGGAGATTGGAGTACCCCTC TATGAATCCCCTCCTGGTAGCAGATACGTGTCCCCGAAGAAGGGAATCAATGAAAATGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211582 representing BC053008 Red=Cloning site Green=Tags(s) MSPAKRWGSPCLFPLQLFSLCWVLSVAQSKTVRYSTFEEDAPGTVIGTLAEDLHMKVSGDTSFRLMKQFN SSLLRVREGDGQLTVGDAGLDRERLCGPSPQCVLAFDVVSFSQEQFRLVHVEVEVRDVNDHAPRFPRAQI PVEVSESAPVGTRIPLEVPVDEDVGANGLQSVRLAEPHSPFRVELQTRADGAQCADLVLLQELDRESQAS YSLELVAQDGGRPPRSATAALSVRVLDANDHSPAFPQGAVAEVELAEDAPVGSLLLDLDAADPDEGPNGD VVFTFGARTPPEARHLFRLDPRSGRLTLAGQVDYERQDTYELDVRAQDRGPGPRTATCKVIVRIRDVNDN APEISITPLAAPGAPATSPFAAAAAAAALGGADAASSTGSGTQEAGITSLVPEGAARESLVALVSTSDRD SGANGQVRCALYGHEHFRLQPAYAGSYLVVTAASLDRERIAEYNLTLVAEDRGTPPLRTVRPYTVRVGDE NDNAPIFTKPVYEVSVRENNPPGAYLATVAARDPDVGRNGQVTYRLVEAEVGRSGEAVSTYVSVDPATGA IYALRSFDYETLRQLDVRVQASDGGSPQLSSNALVQVRVLDQNDHSPILVHPAPANGSLEVAVPGRSTKD TAVARIQARDADEGANGELAFDLLQQEPREAFSIGRHTGEIMLTGDLSQEPPGRVFKALLVISDGGRPPL TTTATVSFVVTAGGGSAVPASSGSPEHSRPPGSRLAPSGPSLQWDTPLIVIIVLAGSCTLLLAAIIAIAT TCNRRKKEVRKGGALREERPGAAGGGASAPGSPDETARGTGPRPNMFDVLTFPGSGKAPFGSPAADAPPP AVAAAEVPGSEGGSATGESACHFEGQQRLRGAHAEPYGASPGFGKEPAAPPVAVWKGHSFNTISGREAEK FSGKDSGKGDSDFNDSDSDISGDALKKDLINHMQSGLWACTAECKILGHSDRCWSPSCAGPNVHPPPHPP AQMSTFCKSTSLPRDPLRRDNYYQAQLPKTVGLQSVYEKVLHRDYDRTVTLLSPPRPGRLPDLQEIGVPL YESPPGSRYVSPKKGINENV TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC053008
ORF Size	3210 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC053008, AAH53008
RefSeq Size	4007 bp
RefSeq ORF	3212 bp
Locus ID	18530
Gene Summary	This gene belongs to the protocadherin gene family, a subfamily of the cadherin superfamily. The gene encodes a type I transmembrane protein composed of an extracellular domain including 6 cadherin ectodomains, a single-pass transmembrane domain and a cytoplasmic tail. Unlike classical cadherins, which are generally encoded by 15-17 exons, this gene includes only 3 exons with the first large exon encoding the extracellular and transmembrane region. Although this gene product is capable of homophilic interaction, it appears to affect cell-cell adhesion indirectly by initiating signaling events that regulate classical cadherin-mediated adhesion. Based on studies on this protein and its orthologs, this protocadherin mainly functions in developing embryos and the central nervous system, but can also function as a tumor suppressor. Alternative splicing yielding isoforms with unique cytoplasmic tails has been reported. [provided by RefSeq, Sep 2009]

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