United States
Germany
Japan
United Kingdom
China
Usp28 (NM_175482) Mouse Tagged ORF Clone
CAT＃: MG211535
- TrueORF®
Usp28 (tGFP-tagged) - Mouse ubiquitin specific peptidase 28 (Usp28)
ORF Plasmid: DDK
"NM_175482" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 10,170.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 9830148O20Rik; AU022237; mKIAA1515 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211535 representing NM_175482
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGCGGAGCTGCAGCAGGACGACTCGGCGGGGGCGGCGGACGGCCATGGCTCGAGCTGCCAGATGC TGTTAAATCAGCTGCGAGAAATCACAGGCATCCAGGATCCTTCCTTTCTTCATGAAGCATTGAAGGCCAG TAATGGTGACATCACCCAGGCTGTCAGCCTTCTCACAGACCAAAGAGTTAAGGAGCCCAGTCACGACACA ACTGCTGCAGAACCATCTGAAGTAGAAGAGAGTGCTACCAGCAAAGATCTCTTAGCAAAAGTGATAGACC TCACTCACGACAACAAAGATGACCTGCAGGCAGCCATTGCCTTGAGTCTCCTGGAGTCTCCTAACATTCA GGCTGACAACAGAGATCTTAATAGAGCGCATGAGGCTAACTCTGCAGAAACTAAACGCTCAAAGAGAAAA CGCTGTGAGGTTTGGGGAGAAAACCACAATCCCAATAACTGGAGGAGAGTGGATGGTTGGCCAGTTGGGC TGAAAAATGTTGGCAACACATGTTGGTTCAGTGCTGTTATTCAGTCTCTCTTTCAGTTGCCTGAATTTCG AAGACTTGTTCTCAGTTATAACCTGCCACAGAACATCCTTGAAAATTGTCGAAGTCACACTGAAAAGAGA AATATCATGTTTATGCAAGAGCTTCAGTATTTATTTGCTCTGTTGTTGGGATCAAATCGAAAATTTGTAG ACCCTTCTGCAGCCCTGGATCTACTGAAGGGAGCCTTCCGATCATCCGAGGAGCAGCAGCAAGATGTGAG TGAATTCACACACAAGCTCCTGGATTGGCTGGAGGATGCGTTCCAGCTAGCTGTTAATGTTAACAGCCAT CTCAGGAATAAATCTGAAAATCCCATGGTGCAGCTATTCTACGGTACTTTTCTGACAGAAGGGGTCCGAG AAGGAAAGCCCTTTTGTAACAATGAGACCTTCGGCCAGTATCCTCTTCAGGTAAACGGTTACCACAACTT AGACGAATGTTTGGAAGGGGCCATGGTGGAAGGTGACATCGCGCTGCTTCCTTCGGATCGTTCCGTGAAG TATGGGCAGGAGCGTTGGTTTACAAAGCTGCCTCCAGTGTTGACCTTTGAACTCTCCAGATTTGAGTTTA ATCAGTCTCTTGGGCAGCCTGAAAAAATTCATAATAAGCTGGAATTTCCTCAGATTATCTATATGGATAG GTACATGTATAAGAGCAAAGAGCTTATCCGAAGTAAGAGAGAGAGCGTTCGGAAGTTGAAGGAGGAAATA CAAGTTCTGCAGCAGAAGTTAGAAAGGTATGTGAAGTACGGCTCGGGCCCATCTCGGTTCCCACTTCCCG ACATGTTGAAATACGTTATTGAATTTGCCAGTACAAAACCTGCCTCTGAAAGCTGTCTGTCTGGAAGTGC TGAGCATGTGACATTACCACTTCCCTCGGTGCACTGCCCGATTTCTGACCTCACACCCAAGGAAAGTTCA AGTCCAGAAAGCTGCTCTCAGAATGCTGGAAGTACCTTTTCCTCTCCAGAAGATGCTCTTCCCAGCTCTG AGGGGATGAATGGGCCATTTACCTCTCCTCACTCGTCTCTGGAAACGCCTGCACCCCCAGCTCCTCGGAC AGTCACAGATGAAGAAATGAACTTCGTTAAGACCTGTCTTCAGAGGTGGCGGAGTGAAATTGAACAGGAT ATACAAGACCTAAAGAACTGTATCTCGAGCAGCACGAAGGCGATTGAGCAGATGTACTGTGACCCTCTTC TTCGTCAGGTGCCTTATCGCTTACATGCAGTTCTTGTTCACGAAGGACAAGCCAGTGCAGGACACTACTG GGCATACATCTACAACCAGCCCCGGCAGACCTGGCTCAAATACAACGACATCTCTGTTACTGAGTCTTCC TGGGAGGAACTTGAAAGAGATTCTTACGGGGGCCTGAGAAATGTCAGCGCCTACTGCTTGATGTATATCA ATGACAACTTGCCCCACTTCAGTGCAGAGGCATCTTCTAATGAATCTGATGAGACTGCAGGAGAAGTGGA AGCCCTGTCTGTGGAACTCAGGCAGTACATTCAGGAAGATAACTGGAGGTTTCAGCAAGAGGTGGAGGAA TGGGAGGAAGAACAGTCCTGTAAGATCCCACAGATGGAGTCCTCCCCGAACTCTTCCTCACAGGATTTCT CCACATCACAAGAGTCTCCAGCAGTGTCCTCTCATGAGGTCCGATGCTTGTCTTCTGAGCATGCTGTGAT TGCAAAAGAACAGACTGCCCAGGCAATTGCCAACACAGCTCATGCCTACGAGAAGAGTGGTGTAGAAGCA GCCTTGAGTGAGGCATTTCATGAGGAGTACTCCAGGCTCTATCAGCTTGCCAAAGAGACACCCACCTCTC ACAGTGACCCTCGCCTTCAGCATGTTCTTGTCTACTTTTTCCAAAATGAAGCACCCAAGAGGGTAGTAGA ACGGACCCTTCTGGAACAGTTTGCGGATAGAAACCTTAGCTATGATGAGAGATCTATCAGTATCATGAAG GTGGCTCAGGCCAAATTGATGGAAATTGGTCCAGATGACATGAACATGGAAGAGTATAAGAGGTGGCATG AAGATTATAGTCTGTTCCGAAAGGTGTCTGTGTACCTTCTAACGGGCCTGGAACTCTTCCAAAAAGGAAA GTACCAGGAAGCATTGTCCTACCTGGTGTACGCATACCAGAGCAACGCTGGCCTGCTGGTGAAGGGGCCC CGAAGGGGCGTGAAGGAGTCTGTGATTGCCTTATACCGCAGAAAATGCCTTCTGGAGCTGAATGCCAAGG CTGCTTCTCTTTTTGAAACAAATGACGACCATTCTGTAACAGAGGGCATTAATGTAATGAATGAGTTGAT CATTCCCTGCATCCACCTGATCATTAATAACGACATCTCCAAGGACGACCTCGATGCCATCGAGGTCATG AGGAACCACTGGTGCTCTTACCTGGGGAAGGATATTGCAGAGAATCTGCAGCTGTGCTTAGGAGAGTTCC TACCCAGGCTTCTAGATCCTTCTGCAGAAATCATCGTCCTAAAGGAGCCTCCAACTATTCGACCGAATTC TCCCTATGATCTTTGCAACCGGTTTGCAGCTGTCATGGAGTCCATTCAAGGAGTTTCAACAGTAACCGTG AAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211535 representing NM_175482
Red=Cloning site Green=Tags(s) MTAELQQDDSAGAADGHGSSCQMLLNQLREITGIQDPSFLHEALKASNGDITQAVSLLTDQRVKEPSHDT TAAEPSEVEESATSKDLLAKVIDLTHDNKDDLQAAIALSLLESPNIQADNRDLNRAHEANSAETKRSKRK RCEVWGENHNPNNWRRVDGWPVGLKNVGNTCWFSAVIQSLFQLPEFRRLVLSYNLPQNILENCRSHTEKR NIMFMQELQYLFALLLGSNRKFVDPSAALDLLKGAFRSSEEQQQDVSEFTHKLLDWLEDAFQLAVNVNSH LRNKSENPMVQLFYGTFLTEGVREGKPFCNNETFGQYPLQVNGYHNLDECLEGAMVEGDIALLPSDRSVK YGQERWFTKLPPVLTFELSRFEFNQSLGQPEKIHNKLEFPQIIYMDRYMYKSKELIRSKRESVRKLKEEI QVLQQKLERYVKYGSGPSRFPLPDMLKYVIEFASTKPASESCLSGSAEHVTLPLPSVHCPISDLTPKESS SPESCSQNAGSTFSSPEDALPSSEGMNGPFTSPHSSLETPAPPAPRTVTDEEMNFVKTCLQRWRSEIEQD IQDLKNCISSSTKAIEQMYCDPLLRQVPYRLHAVLVHEGQASAGHYWAYIYNQPRQTWLKYNDISVTESS WEELERDSYGGLRNVSAYCLMYINDNLPHFSAEASSNESDETAGEVEALSVELRQYIQEDNWRFQQEVEE WEEEQSCKIPQMESSPNSSSQDFSTSQESPAVSSHEVRCLSSEHAVIAKEQTAQAIANTAHAYEKSGVEA ALSEAFHEEYSRLYQLAKETPTSHSDPRLQHVLVYFFQNEAPKRVVERTLLEQFADRNLSYDERSISIMK VAQAKLMEIGPDDMNMEEYKRWHEDYSLFRKVSVYLLTGLELFQKGKYQEALSYLVYAYQSNAGLLVKGP RRGVKESVIALYRRKCLLELNAKAASLFETNDDHSVTEGINVMNELIIPCIHLIINNDISKDDLDAIEVM RNHWCSYLGKDIAENLQLCLGEFLPRLLDPSAEIIVLKEPPTIRPNSPYDLCNRFAAVMESIQGVSTVTV K TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175482 |
| ORF Size | 3153 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175482.3, NP_780691.2 |
| RefSeq Size | 4220 bp |
| RefSeq ORF | 3156 bp |
| Locus ID | 235323 |
| UniProt ID | Q5I043 |
| Gene Summary | Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBXW7) complex. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223359 | Usp28 (untagged) - Mouse ubiquitin specific peptidase 28 (Usp28), (10ug) |
CNY 13,490.00 |
|
| MR211535 | Usp28 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 28 (Usp28) |
CNY 7,320.00 |
|
| MR211535L3 | Lenti ORF clone of Usp28 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 28 (Usp28) |
CNY 11,120.00 |
|
| MR211535L4 | Lenti ORF clone of Usp28 (mGFP-tagged) - Mouse ubiquitin specific peptidase 28 (Usp28) |
CNY 11,120.00 |
