Herc4 (BC043082) Mouse Tagged ORF Clone

CAT#: MG211530

  • TrueORF®

Herc4 (tGFP-tagged) - Mouse hect domain and RLD 4 (cDNA clone MGC:57994 IMAGE:6417111)



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Mouse Protein / Lysate Request

CNY 9,220.00


货期*
7周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 1700056O17Rik; 4921531D01Rik; 9530080M15Rik; mKIAA1593
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG211530 representing BC043082
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTGTGCTGGGGCAATGCATCCTATGGACAACTAGGTTTGGGAGGAATTGATGAAGAAATTGTACTAG
AGCCCAGGAGAAGTGACTTTTTCGTGAACAAAAAGGTCCGAGATGTAGGCTGTGGACTCAGGCACACTGT
GTTTGTCCTGGATGATGGGACTGTGTACACATGTGGATGTAATGATCTAGGACAGCTAGGTCATGAAAAG
TCCAGAAAGAAACCAGAGCAGGTTGTTGCCCTGGATGCCCAGAATATCGTAGCTGTTGCGTGTGGAGAAG
CTCACACGTTAGCGCTGAATGACAAGGGCCAGGTGTATGCTTGGGGTCTCGACTCTGACGGACAGCTTGG
CCTACAGGGATCAGAGGAATGTATCAGAGTACCCAGAAATATTAAAAGTCTTTCGGATATCCAGATAGTA
CAGGTTGCGTGTGGTTACTATCATTCGCTTGCACTTTCTAAAGCAAGTGAAGTTTTCTGTTGGGGACAGA
ATAAATATGGCCAGCTGGGTCTAGGCATTGATTGTCAAAAGCAAACTTCACCACAGCTGATTAAGTCTTT
GCTTGGAATACCATTCATGCAAGTCGCAGCAGGAGGAGCCCATAGTTTTGTACTCACCCTTTCCGGAGCT
ATCTTTGGATGGGGACGTAACAAATTTGGTCAGCTAGGTCTTAATGATGAAAATGATAGGTATGTTCCTA
ATTTACTAAAGTCACTAAGATCTCAGAAAATAGTTTATATTTGTTGCGGAGAAGATCATACTGCTGCATT
AACCAAGGAAGGTGGAGTGTTTACCTTTGGAGCTGGGGGCTATGGCCAGCTGGGTCATAATTCTACCAGT
CATGAGATAAATCCCAGGAAAGTTTTTGAACTCATGGGAAGCATTGTTACTCAGGTCGCTTGTGGAAGGC
AGCATACCTCTGCATTTGTTCCTTCTTCAGGACGAATTTACTCCTTTGGTCTTGGTGGTAATGGGCAGTT
GGGAACTGGTTCAACCAGCAACAGAAAAAGCCCTTTCACTGTAAAAGGAAACTGGTTTTCTTATAATGGA
CAGTGTCCACAAGATATTGGTTCTGAAGACTATTTCTGTGTCAAAAGAATTTTCTCAGGTGGAGATCAAA
GCTTTTCACATTACTCTAGTCCTCAGAACTGTGGACCTCCAGATGACTTTAGATGCTCCGACCCTTCCAA
GCAGATCTGGACAGTGAACGAAGCTCTGATTCAGAAATGGCTCAGCTACCCCTCTGGAAGGTTCCCTGTG
GAGATAGCCAATGAGATAGATGGAACATTTTCTTCATCTGGCTGCCTAAATGGAAGTTTTCTGGCTATTA
GCAATGACGATCACTATAGAACAGGTACCAGATTTTCAGGGGTTGATATGAACGCTGCTAGACTCTTATT
CCACAAACTTATACAACCTGATCATCCTCAGATATCTCAGCAGGTGGCAGCTAGTTTGGAAAAGAATCTA
ATCCCTAAACTGACTAGTTCCCTACCTGATGTTGAAGCTCTGAGGTTTTATCTTACCCTGCCAGAGTGTC
CCCTGATGAGTGACTGCAACAATTTCACAACAATAGCAATCCCCTTTGGTACAGCTCTTGTGAACCTAGA
AAAGGCACCACTGAAAGTACTTGAAAACTGGTGGTCAGTACTTGAACCTCCACTATTCCTCAAGATAGTA
GAACTTTTTAAGGAAGTTGTGGTACATCTTTTGAAACTCTACAAGATCGGCATTCCCCCTTCTGAAAGAA
GAATTTTCAACAGTTTTCTTCATACCGCATTAAAGGTTTTAGAAATATTGCATAGGGTAAATGAGAAAAC
AGGACAACTTATTCAATATGACAAGTTTTACATCCATGAAGTCCAAGAGCTGATAGACATAAGGAATGAC
TATATCAACTGGGTCCAGCAGCAAGCCTATGGAGTGTTGGCAGATATCCCGGTGACAATTTGTACATATC
CATTTGTATTTGATGCCCAGGCAAAAACTACTCTGCTCCAGACAGATGCAGTCTTGCAGATGCAGATGGC
CATTGACCAGGCCCACAGGCAGAATGTCTCCTCTCTTTTTCTCCCAGTGATCGAGTCTGTGAATCCTTGC
TTAATTCTAGTTGTTCGCAGAGAGAATATTGTAGGAGATGCGATGGAAGTCCTCAGGAAAACCAAGAATA
TAGATTATAAAAAGCCACTCAAGGTTATATTTGTTGGAGAAGATGCTGTTGATGCAGGAGGTGTTCGCAA
AGAATTTTTCTTGCTTATCATGAGGGAATTGTTGGATCCTAAATACGGCATGTTTCGATATTATGAAGAT
TCCAGGCTCATTTGGTTTTCAGATAAGACATTTGAAGACAGTGATTTGTTCCACTTGATCGGTGTTATCT
GTGGATTAGCAATTTATAATTTTACCATTGTGGACCTCCATTTCCCTTTGGCTTTATATAAGAAACTGCT
GAAAAGGAAGCCGTCCCTGGATGATCTGAAAGAGCTGATGCCAGCCGTCGGGAGAAGCATGCAGCAATTG
CTGGACTATCCAGAAGATGACATAGAGGAAACATTTTGTCTAAACTTTACGATCACAGTTGAGAACTTCG
GTGCAACAGAAGTAAAAGAGCTGGTTCTGAACGGGGCAGACACTGCTGTTAATAGACAGAATCGGCAGGA
GTTTGTTGATGCCTATGTGGATTACATATTCAATAAATCAGTGGCATCTTTATTTGATGCTTTCCATGCG
GGCTTTCATAAGGTCTGTGGAGGAAAAGTTCTTCTGCTCTTCCAGCCTAATGAATTACAAGCTATGGTTA
TTGGGAATACAAATTATGATTGGAAAGAACTGGAGAAGAATACAGAATACAAAGGAGAGTATTGGGCAGA
CCATCCCACGATAAAAATTTTTTGGGAAGTGTTTCATGAATTACCACTAGAGAAGAAGAAACAATTTCTA
TTATTTTTGACAGGTAGTGATCGGATTCCCATTCTTGGTATGAAGAGTCTGAAACTAGTCATCCAGTCAA
CAGGAGGTGGTGAGAGCTATCTCCCGGTTTCCCATACCTGTTTTAATCTTCTAGATCTCCCAAAATATAC
AGAGAAAGAAACCCTCCGATGTAAACTGATCCAAGCTATTGATCACAATGAAGGCTTCAGTTTAATA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG211530 representing BC043082
Red=Cloning site Green=Tags(s)

MLCWGNASYGQLGLGGIDEEIVLEPRRSDFFVNKKVRDVGCGLRHTVFVLDDGTVYTCGCNDLGQLGHEK
SRKKPEQVVALDAQNIVAVACGEAHTLALNDKGQVYAWGLDSDGQLGLQGSEECIRVPRNIKSLSDIQIV
QVACGYYHSLALSKASEVFCWGQNKYGQLGLGIDCQKQTSPQLIKSLLGIPFMQVAAGGAHSFVLTLSGA
IFGWGRNKFGQLGLNDENDRYVPNLLKSLRSQKIVYICCGEDHTAALTKEGGVFTFGAGGYGQLGHNSTS
HEINPRKVFELMGSIVTQVACGRQHTSAFVPSSGRIYSFGLGGNGQLGTGSTSNRKSPFTVKGNWFSYNG
QCPQDIGSEDYFCVKRIFSGGDQSFSHYSSPQNCGPPDDFRCSDPSKQIWTVNEALIQKWLSYPSGRFPV
EIANEIDGTFSSSGCLNGSFLAISNDDHYRTGTRFSGVDMNAARLLFHKLIQPDHPQISQQVAASLEKNL
IPKLTSSLPDVEALRFYLTLPECPLMSDCNNFTTIAIPFGTALVNLEKAPLKVLENWWSVLEPPLFLKIV
ELFKEVVVHLLKLYKIGIPPSERRIFNSFLHTALKVLEILHRVNEKTGQLIQYDKFYIHEVQELIDIRND
YINWVQQQAYGVLADIPVTICTYPFVFDAQAKTTLLQTDAVLQMQMAIDQAHRQNVSSLFLPVIESVNPC
LILVVRRENIVGDAMEVLRKTKNIDYKKPLKVIFVGEDAVDAGGVRKEFFLLIMRELLDPKYGMFRYYED
SRLIWFSDKTFEDSDLFHLIGVICGLAIYNFTIVDLHFPLALYKKLLKRKPSLDDLKELMPAVGRSMQQL
LDYPEDDIEETFCLNFTITVENFGATEVKELVLNGADTAVNRQNRQEFVDAYVDYIFNKSVASLFDAFHA
GFHKVCGGKVLLLFQPNELQAMVIGNTNYDWKELEKNTEYKGEYWADHPTIKIFWEVFHELPLEKKKQFL
LFLTGSDRIPILGMKSLKLVIQSTGGGESYLPVSHTCFNLLDLPKYTEKETLRCKLIQAIDHNEGFSLI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC043082
ORF Size 3149 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC043082, AAH43082
RefSeq Size 3857 bp
RefSeq ORF 3149 bp
Locus ID 67345
Gene Summary Probable E3 ubiquitin-protein ligase involved in either protein trafficking or in the distribution of cellular structures. Required for spermatozoon maturation and fertility, and for the removal of the cytoplasmic droplet of the spermatozoon. E3 ubiquitin-protein ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfer it to targeted substrates.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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