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Ulk2 (BC053029) Mouse Tagged ORF Clone
CAT＃: MG211498
- TrueORF®
Ulk2 (tGFP-tagged) - Mouse Unc-51 like kinase 2 (C. elegans) (cDNA clone MGC:62255 IMAGE:5709559)
ORF Plasmid: DDK
"BC053029" in other vectors (4)
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CNY 11,400.00
Product images
CNY 1,000.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | Unc51.2, mKIAA0623 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211498 representing BC053029
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGTGGTGGGCGACTTCGAGTACTGCAAGCGGGACCTCGTGGGACACGGGGCCTTCGCTGTGGTCT TCCGGGGGCGGCACCGCCAGAAAACTGATTGGGAGGTGGCTATTAAAAGTATTAATAAAAAGAACTTGTC AAAATCACAAATTCTGCTTGGAAAGGAAATAAAAATCTTAAAGGAGCTTCAGCATGAAAACATCGTAGCG CTCTATGATGTTCAGGAATTGCCCAACTCTGTCTTTCTGGTGATGGAGTATTGCAATGGTGGAGACCTGG CAGATTATTTGCAAGCTAAAGGAACTCTGAGTGAAGATACTATCAGAGTGTTTCTCCATCAGATTGCGGC AGCCATGCGAATCCTGCACAGCAAAGGGATAATCCACAGGGATCTCAAACCACAGAATATCCTGTTGTCT TATGCCAATCGAAGGAAGTCGAATGTCAGTGGTATTCGTATTAAAATAGCTGATTTTGGTTTCGCACGGT ACCTACATAGTAACACAATGGCAGCGACACTGTGTGGATCCCCAATGTACATGGCTCCCGAGGTTATTAT GTCTCAACATTATGATGCTAAGGCAGATTTATGGAGCATAGGAACAGTGATCTATCAATGCCTAGTTGGA AAACCACCTTTTCAGGCTAATAGTCCTCAGGACCTAAGGATGTTTTATGAAAAAAACAGGAGCTTAATGC CTAGTATTCCCAGAGAAACATCACCTTACTTGGCTAATCTCCTTTTGGGTTTGCTTCAGAGAAATCAAAA GGATAGAATGGACTTTGAAGCATTTTTCAGCCATCCTTTCCTTGAGCAAGTTCCAGTTAAAAAATCTTGC CCAGTCCCAGTGCCTGTGTATTCTGGCCCTGTCCCTGGAAGCTCCTGCAGCAGCTCACCATCTTGTCGCT TTGCTTCTCCACCATCCCTTCCAGATATGCAGCATATTCAGGAAGAAAACTTATCCTCCCCACCGTTGGG TCCTCCCAACTATCTACAGGTGTCCAAAGACTCTGCGAGTAATAGTAGCAAGAACTCTTCTTGTGACACG GATGACTTTGTTTTGGTTCCACACAACATCTCGTCAGACCACTCATATGACATGCCAATGGGGACTACGG CCAGACGTGCTTCAAATGAATTCTTTATGTGTGGAGGGCAGTGTCAACCTACTGTGTCACCTCACAGCGA AACAGCCCCAATTCCAGTTCCTACTCAAGTAAGGAATTATCAGCGCATAGAACAGAATCTTATATCCACT GCCAGCTCTGGCACAAACCCACATGGTTCTCCAAGATCTGCAGTAGTACGAAGGTCTAATACCAGCCCCA TGGGCTTCCTCCGGGTTGGGTCCTGCTCCCCTGTACCAGGAGACACAGTGCAGACAGGAGGACGAAGACT CTCTACTGGCTCTTCCAGGCCTTACTCACCATCCCCTTTGGTTGGTACCATTCCTGAACAGTTTAGTCAG TGCTGCTGTGGACATCCTCAGGGCCATGAAGCCAGGAGTAGGCACTCCTCAGGTTCTCCAGTGCCACAGA CCCAGGCACCACAGTCACTCTTACTGGGTGCTAGACTGCAGAGTGCACCCACCCTCACCGATATCTATCA GAACAAGCAGAAGCTCAGAAAGCAGCACTCTGACCCTGTGTGTCCGTCCCATGCTGGAGCTGGGTATAGT TACTCACCTCAGCCTAGTCGGCCTGGCAGCCTTGGGACCTCTCCCACCAAGCACACGGGGTCCTCTCCAC GGAATTCTGACTGGTTCTTTAAAACTCCTTTACCAACAATCATTGGCTCTCCTACTAAGACTACAGCTCC TTTCAAAATCCCTAAAACACAAGCATCTTCTAACCTGTTAGCCTTGGTTACTCGTCATGGGCCTGCTGAA AGCCAGTCCAAAGATGGGAATGACCCTCGTGAGTGTTCCCACTGCCTCTCAGTACAAGGAAGCGAGAGGC ATCGATCTGAGCAGCAGCAGAGCAAGGCAGTGTTTGGCAGATCTGTCAGTACTGGGAAGTTATCAGAACA ACAAGTAAAGGCACCTTTAGGTGGACACCAGGGCAGCACGGATAGTTTAAACACAGAACGACCAATGGAT GTAGCTCCTGCAGGAGCCTGTGGTGTTATGCTGGCATTGCCAGCAGGAACAGCAGCAAGCGCCAGAGCTG TCCTCTTCACCGTGGGGTCTCCTCCACACAGTGCCACAGCCCCCACTTGTACTCATATGGTCCTTCGAAC AAGAACCACCTCAGTGGGGTCCAGCAGCTCAGGAGGTTCCTTGTGTTCTGCAAGTGGCCGAGTATGTGTG GGCTCCCCTCCTGGACCAGGGTTGGGCTCTTCCCCACCAGGAGCAGAGGGAGCTCCCAGCCTAAGATACG TGCCTTATGGTGCTTCACCACCCAGCCTAGAGGGTCTCATCACCTTTGAAGCCCCTGAACTACCAGAGGA GACACTGATGGAGCGAGAGCACACAGACACCTTACGCCATCTGAACATGATGTTAATGTTTACTGAGTGT GTGCTGGACCTGACGGCAGTGAGGGGTGGGAACCCTGAGCTGTGCACATCTGCTGTGTCCTTGTACCAGA TTCAGGAGAGTGTAGTTGTGGACCAGATCAGCCAGCTAAGCAAAGATTGGGGGCGGGTGGAGCAGCTGGT GTTGTACATGAAGGCAGCACAGCTGCTGGCGGCTTCCCTGCATCTCGCCAAAGCTCAGGTCAAGTCTGGG AAGCTGAGCCCATCCATGGCTGTGAAACAAGTTGTTAAAAATCTGAATGAAAGATACAAATTCTGCATCA CCATGTGCAAGAAACTTACAGAAAAGCTGAATCGCTTCTTCTCCGATAAACAGAGATTTATTGATGAAAT CAACAGTGTGACTGCAGAGAAACTCATCTATAATTGTGCTGTGGAAATGGTTCAATCTGCAGCCCTGGAT GAGATGTTTCAGCAGACTGAAGACATCGTTTATCGCTACCACAAGGCAGCCCTTCTTTTGGAAGGCTTAA GTAAGATCCTGCAGGACCCTACAGATGTTGAAAATGTGCATAAGTATAAATGTAGTATTGAAAGAAGATT GTCAGCACTCTGCTGTAGCACTGCAACTGTG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211498 representing BC053029
Red=Cloning site Green=Tags(s) MEVVGDFEYCKRDLVGHGAFAVVFRGRHRQKTDWEVAIKSINKKNLSKSQILLGKEIKILKELQHENIVA LYDVQELPNSVFLVMEYCNGGDLADYLQAKGTLSEDTIRVFLHQIAAAMRILHSKGIIHRDLKPQNILLS YANRRKSNVSGIRIKIADFGFARYLHSNTMAATLCGSPMYMAPEVIMSQHYDAKADLWSIGTVIYQCLVG KPPFQANSPQDLRMFYEKNRSLMPSIPRETSPYLANLLLGLLQRNQKDRMDFEAFFSHPFLEQVPVKKSC PVPVPVYSGPVPGSSCSSSPSCRFASPPSLPDMQHIQEENLSSPPLGPPNYLQVSKDSASNSSKNSSCDT DDFVLVPHNISSDHSYDMPMGTTARRASNEFFMCGGQCQPTVSPHSETAPIPVPTQVRNYQRIEQNLIST ASSGTNPHGSPRSAVVRRSNTSPMGFLRVGSCSPVPGDTVQTGGRRLSTGSSRPYSPSPLVGTIPEQFSQ CCCGHPQGHEARSRHSSGSPVPQTQAPQSLLLGARLQSAPTLTDIYQNKQKLRKQHSDPVCPSHAGAGYS YSPQPSRPGSLGTSPTKHTGSSPRNSDWFFKTPLPTIIGSPTKTTAPFKIPKTQASSNLLALVTRHGPAE SQSKDGNDPRECSHCLSVQGSERHRSEQQQSKAVFGRSVSTGKLSEQQVKAPLGGHQGSTDSLNTERPMD VAPAGACGVMLALPAGTAASARAVLFTVGSPPHSATAPTCTHMVLRTRTTSVGSSSSGGSLCSASGRVCV GSPPGPGLGSSPPGAEGAPSLRYVPYGASPPSLEGLITFEAPELPEETLMEREHTDTLRHLNMMLMFTEC VLDLTAVRGGNPELCTSAVSLYQIQESVVVDQISQLSKDWGRVEQLVLYMKAAQLLAASLHLAKAQVKSG KLSPSMAVKQVVKNLNERYKFCITMCKKLTEKLNRFFSDKQRFIDEINSVTAEKLIYNCAVEMVQSAALD EMFQQTEDIVYRYHKAALLLEGLSKILQDPTDVENVHKYKCSIERRLSALCCSTATV TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | BC053029 |
| ORF Size | 3111 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | BC053029.1 |
| RefSeq Size | 4413 bp |
| RefSeq ORF | 3113 bp |
| Locus ID | 29869 |
| Gene Summary | Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and a negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK, also acts as a negative regulator of AMPK through phosphorylation of the AMPK subunits PRKAA1, PRKAB2 and PRKAG1. May phosphorylate ATG13/KIAA0652, FRS2, FRS3 and RPTOR; however such data need additional evidences. Not involved in ammonia-induced autophagy or in autophagic response of cerebellar granule neurons (CGN) to low potassium concentration. Plays a role early in neuronal differentiation and is required for granule cell axon formation: may govern axon formation via Ras-like GTPase signaling and through regulation of the Rab5-mediated endocytic pathways within developing axons.[UniProtKB/Swiss-Prot Function] |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223082 | Ulk2 (untagged) - Mouse Unc-51 like kinase 2 (C. elegans) (cDNA clone MGC:62255 IMAGE:5709559), (10ug) |
CNY 12,920.00 |
|
| MR211498 | Ulk2 (Myc-DDK-tagged) - Mouse Unc-51 like kinase 2 (C. elegans) (cDNA clone MGC:62255 IMAGE:5709559) |
CNY 7,224.00 |
|
| MR211498L3 | Lenti ORF clone of Ulk2 (Myc-DDK-tagged) - Mouse Unc-51 like kinase 2 (C. elegans) (cDNA clone MGC:62255 IMAGE:5709559) |
CNY 12,260.00 |
|
| MR211498L4 | Lenti ORF clone of Ulk2 (mGFP-tagged) - Mouse Unc-51 like kinase 2 (C. elegans) (cDNA clone MGC:62255 IMAGE:5709559) |
CNY 12,260.00 |
