Senp7 (BC058593) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	mKIAA1707
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211497 representing BC058593 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACAGGGCCAGGCCTGGGCGTCGGCGAGCTTCATCAGAGATTGTTACAGAGGGAAAACGGAAAAAGT CATCACCAGCTGATTTACAGAAGATAACAAAGTTGTTAACTGTAAAGTCAGAGGATGTTCTTGCTCAGTC ACCATTGTCCAAACTCAGAGGCTCAGAATGCTGGTGGACAAGAAGCCTAAGAAATAAAGTCATCTGTCTA GACCACAAAAAACCAAAAGCTGCCCGTGGGTGTCCTCCTAAGGGATTACCAAAAAGGCATCTCAGAGTTA TGTTGACGAATGTTCTATGGACGGACTTAGGACGAGAATTCAGAAAGACCCTGCCTAGAAAGGATGCTAA TTTATGTGCTCCCAGCAAGGTGCAATCAGACTCATTGCCTTCGACATCTGTTGACAGCATAGAGACATGT CAAAGATTAGATCCTCTTCACCAAAGCCTTAATTTATCCGAAAGGACACCCAGAGTTATACTGACGGATA TCCGGCAAACAGAATTAGGAAGAAAATATTTAAAGATCCCACCTGTAACTGAGGCCAGTTTGAGTGATAC AGCCAACCTGAAATCAGAGCAACTTTCTTCATCATCTGATGGCAGCTTAGAGTCTTGTCAGAGTGTAAAT CATCACAAGAGCTTTTTATCTGAAAGTGGTCCCAAACCAAGTAGGACAGGTGACGTTCCTGCAAAGGAGG CTGCATGTGGGGGACAGAAGCAGGGTGATGATGGAGGAGTCACTCCTGAGATGGCTGCTCCTCATCCTAA AGACTTTAATACTGGAAATAAAGGTTGTGATTATCTTGAAGAGGGGACCAGCAACAAGAATACTTCATAT TCTTATTCGGAAATGGACCATACTCCAGTTTCCAGGAAGAGAAAGAAAAGGGGTAGAAGTAATTTTCATG ATTCTCATAATTCTAAGACTTCTTTGGATAAGCCAACAGAACATACAAAAGAAGAAGAAAATGACTCTTC AGTCTCCCGTAAGCTTGAAGAGTCCGGTGAAGACTCTCACCAGGACCCAGCACCACCCGAAGGACTCGCG CCAGAATCTTTAGAGAGCGAGGCCACTAACCTGCGCTCATTCGCTGCTGGTCAGGAGCCAGATGCTAGCG CTGCCTCTGGAAGGGCCTCTTCACCCAACAAAAGCTTGGAGAGCTCTGCTTCTAGTGAAGTGTCGGAGAA TTCTTCCGTGGCTGTGAAGGGTGAGGCGTTGACTCTAAAAGAAGCATCCCCTCCGGGTGGAAGCAGTGAA GAGAGCCAGTTGTTGATCTCAGCTGAGCCAATTGTAGTTTCCAGTGATGAAGAAGGACCGGTTGAACATA AGAATTCAGTAATTCTAAAATTACAGCCTCCTCATGAGATTATGAGTGAAAATCAGGGCACTTCTGACCC ACAGTTGTCAGAGCTGACACTCGGTGCATGTGAATCTGTACAGGTAACATCTGAATTATTTCCATACAAT CCTGATGTAGAAAACATTTCCTGTATTAAATCTAATAGTGAAATGGATCTGAAATTGGATTTCATATTTA CATGTGTGTATATTGGTAAAATAAAAGGAACTCCAAAAGGTTGTGTCACGTTCACAAAGAAGTATATTAA AATCCCGTTTCAAGTGTCCACAAATGAGATTTCCCTGACAGTGGACACCGCACGTTTAAAGAGGTTCGGG TTATGGGAAAGCAAGGATGAGGACCACAGCAAGAGGAGCCACGCTATCCTTTTCCTCTGGCTTTCCTCAG ACTACCTTCAAGACATTCAGACCCAGCTAGAAAACCCCATGCTAAGCCAGCAATCAAAAGCCAATGAATT TATTTTCCTTGAGTTAAACAGTTCCATTTCACAAAGAGAAGAATTGAAATTGAAAGATATTATGATGGAA ATCAGTACAAAAAATGGAAATTTACACCTGTCTTGTCCATTGCCCTGGGTCCAGGCACTTCCTTTGTTTC AGGATCTCTCTCCACAAGAAATCTCTTTTCTTCACTATTACTATGCTTCAGCTTCTGCTCTCCCCACTGC TGCTGGGGCAGACATGAAGAAGAAGTCAGTGTCTCAGCCCTCAAATTCAGACACCATCAAGCCTACTTAC ACCTTCCTACATAAACAAAGTAGTGGTTGCTACTCGCTCTCTATTACTTCAAGTCCAGAGGAGGAATGGC AGGAAGTCAGGAACACGGGACCCGTTCAGAAGTTGATTGTTTATCCTCCACCACCTACTAAGGGAGGCTT GGGAGTAACTAATGAAGATCTAGAGTGTTTAGAAGAAGGAGAATTTCTTAACGATGTAATCATTGACTTT TACCTTAAGTATCTTCTATTGGAGAAGGCATCAGATGAACTTGTTGAACGAAGTCACATTTTTAGTAGCT TTTTCTATAAATGCTTGACAAGAAAGGAAAATAATTTAACAGAAGATAATCCAGATCTATCAGTGGCTCA GAGGAGACACCGAAGAGTGAGAACATGGACTCGTCATATAAACATTTTTAATAAAGACTACATCTTTGTA CCTGTTAATGAATCGTCTCACTGGTATCTTGCAGTCATTTGTTTCCCATGGCTAGAAGAGGCTGTGTATG AAGATTGTCCACAAACTGTGTCCCAACAGTTCCAGGGTCAGCAGTCTCAGCATGACCACAAAATGACAGA TAATGATCCACATACCACTTCAACTGTGTCCACGAGTGCAGAGGATTCCCAAAGTACCGAGGTGAATATG TCAGTACCAAAGAAAATGTGTAAGCGGCCATGTATTCTCATACTAGACTCCCTGAAAGCTGCTTCTATAC AAAACACAGTTCAGAACTTACGAGAGTATTTGGAGGTAGAGTGGGAGGTGAAACGAAAAACGCACCGTGA ATTCAGTAAAACCAATATGGTCGACCTGTGCCCCAAAGTCCCTAAGCAGGATAACAGCAGTGACTGTGGA GTCTATTTGTTGCAGTATGTGGAGAGCTTTTTCCAGGATCCCATTGTTAACTTTGAACTTCCAATTCACT TGGAGAAATGGTTTCCTCGCCATGTAATAAAGACCAAACGGGAAGATATTCGAGAGCTCATTTTGAAACT TCACTTACAGCAACAGAAGGGCGGCAGCTGC ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211497 representing BC058593 Red=Cloning site Green=Tags(s) MDRARPGRRRASSEIVTEGKRKKSSPADLQKITKLLTVKSEDVLAQSPLSKLRGSECWWTRSLRNKVICL DHKKPKAARGCPPKGLPKRHLRVMLTNVLWTDLGREFRKTLPRKDANLCAPSKVQSDSLPSTSVDSIETC QRLDPLHQSLNLSERTPRVILTDIRQTELGRKYLKIPPVTEASLSDTANLKSEQLSSSSDGSLESCQSVN HHKSFLSESGPKPSRTGDVPAKEAACGGQKQGDDGGVTPEMAAPHPKDFNTGNKGCDYLEEGTSNKNTSY SYSEMDHTPVSRKRKKRGRSNFHDSHNSKTSLDKPTEHTKEEENDSSVSRKLEESGEDSHQDPAPPEGLA PESLESEATNLRSFAAGQEPDASAASGRASSPNKSLESSASSEVSENSSVAVKGEALTLKEASPPGGSSE ESQLLISAEPIVVSSDEEGPVEHKNSVILKLQPPHEIMSENQGTSDPQLSELTLGACESVQVTSELFPYN PDVENISCIKSNSEMDLKLDFIFTCVYIGKIKGTPKGCVTFTKKYIKIPFQVSTNEISLTVDTARLKRFG LWESKDEDHSKRSHAILFLWLSSDYLQDIQTQLENPMLSQQSKANEFIFLELNSSISQREELKLKDIMME ISTKNGNLHLSCPLPWVQALPLFQDLSPQEISFLHYYYASASALPTAAGADMKKKSVSQPSNSDTIKPTY TFLHKQSSGCYSLSITSSPEEEWQEVRNTGPVQKLIVYPPPPTKGGLGVTNEDLECLEEGEFLNDVIIDF YLKYLLLEKASDELVERSHIFSSFFYKCLTRKENNLTEDNPDLSVAQRRHRRVRTWTRHINIFNKDYIFV PVNESSHWYLAVICFPWLEEAVYEDCPQTVSQQFQGQQSQHDHKMTDNDPHTTSTVSTSAEDSQSTEVNM SVPKKMCKRPCILILDSLKAASIQNTVQNLREYLEVEWEVKRKTHREFSKTNMVDLCPKVPKQDNSSDCG VYLLQYVESFFQDPIVNFELPIHLEKWFPRHVIKTKREDIRELILKLHLQQQKGGSC TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC058593
ORF Size	3111 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC058593, AAH58593
RefSeq Size	4788 bp
RefSeq ORF	3113 bp
Locus ID	66315
Gene Summary	This gene encodes a SUMO deconjugating enzyme of the Sentrin/SUMO-specific protease (SENP) family. The encoded protein is a protease that exhibits deSUMOylating activity towards proteins involved in chromatin remodeling and promotes chromatin relaxation for DNA repair or transcription. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2015]

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