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Parp1 (NM_007415) Mouse Tagged ORF Clone
CAT＃: MG211449
- TrueORF®
Parp1 (tGFP-tagged) - Mouse poly (ADP-ribose) polymerase family, member 1 (Parp1)
ORF Plasmid: DDK
"NM_007415" in other vectors (4)
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CNY 12,192.00
| Cited in 1 publication. |
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | 5830444G22Rik; Adprp; Adprt1; AI893648; ARTD1; C80510; PARP; parp-1; PPOL; sPARP-1 |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG211449 representing NM_007415
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGGCCTCGGAGAGGCTTTATCGAGTGGAGTACGCGAAGAGCGGGCGCGCCTCTTGCAAGAAAT GCAGCGAGAGTATTCCCAAGGACTCCCTCCGCATGGCCATCATGGTGCAGTCACCCATGTTCGATGGGAA AGTCCCACACTGGTACCACTTCTCCTGCTTCTGGAAGGTGGGCCACTCCATCCGGCAGCCTGATGTTGAG GTGGATGGCTTCTCTGAGCTGCGCTGGGATGATCAGCAGAAGGTCAAGAAGACGGCCGAGGCTGGAGGCG TGGCAGGCAAAGGCCAGGATGGAAGTGGCGGCAAGGCGGAGAAGACATTGGGTGACTTTTTAGCGGAGTA CGCCAAGTCCAACAGGAGCATGTGCAAGGGCTGCCTGGAGAAGATAGAGAAGGGCCAGATGCGCCTGTCC AAGAAGATGGTGGATCCAGAGAAGCCACAGCTGGGTATGATTGACCGCTGGTACCATCCAACTTGCTTTG TCAAGAAGCGGGACGAGCTGGGCTTCCGGCCTGAGTACAGTGCCAGTCAGCTCAAGGGCTTTAGCCTCCT CTCTGCAGAAGACAAAGAAGCTCTGAAGAAGCAGCTCCCGGCCATCAAGAATGAAGGAAAGAGAAAAGGT GACGAGGTGGATGGAACAGATGAAGTGGCCAAAAAGAAATCTAAGAAAGGGAAGGACAAGGATAGTAGTA AGCTGGAGAAGGCCCTCAAGGCTCAGAATGAGCTGATCTGGAATATCAAAGACGAGCTGAAGAAAGCGTG TTCCACCAACGACCTGAAGGAGCTGCTCATCTTCAACCAGCAGCAGGTGCCGTCAGGAGAGTCAGCGATC TTGGACAGAGTTGCTGACGGCATGGCGTTTGGGGCCCTTCTGCCCTGCAAGGAGTGTTCAGGCCAGCTGG TCTTTAAGAGCGACGCTTATTACTGTACTGGGGATGTCACTGCCTGGACCAAGTGCATGGTCAAGACACA GAATCCTAGCCGAAAGGAATGGGTAACTCCAAAGGAATTCCGAGAAATATCCTACCTCAAGAAGTTAAAG GTCAAAAAACAGGACCGAATATTCCCTCCAGAAAGCAGCGCCCCAGCACCACTGGCACTGCCCCTCTCTG TCACCTCAGCACCCACAGCTGTGAACTCCTCTGCTCCAGCAGACAAGCCCCTGTCTAACATGAAGATCCT GACTCTTGGGAAGCTCTCCCAGAACAAGGACGAAGCAAAAGCTGTGATTGAGAAACTCGGAGGCAAGTTG ACAGGATCTGCCAACAAGGCCTCCTTGTGTATCAGCACTAAAAAGGAGGTGGAGAAGATGAGTAAGAAGA TGGAGGAAGTGAAAGCGGCCAACGTTCGAGTTGTGTGTGAGGACTTCCTCCAGGACGTGTCTGCCTCCAC TAAAAGCCTCCAAGAGCTGCTCTCGGCCCACAGCTTGTCCTCGTGGGGGGCTGAGGTGAAGGCAGAGCCT GGTGAAGTGGTGGCCCCCAAGGGGAAGTCAGCTGCACCCTCCAAGAAGAGCAAGGGTGCTGTCAAGGAGG AAGGTGTCAACAAATCTGAAAAGAGGATGAAATTAACTCTGAAGGGAGGAGCAGCCGTTGATCCTGACTC TGGTCTGGAACACTCTGCACACGTCCTGGAGAAAGGTGGGAAGGTGTTCAGCGCCACACTTGGCCTGGTG GACATTGTGAAAGGGACGAACTCCTATTACAAACTGCAGCTTCTGGAGGACGACAAGGAGAGCAGGTACT GGATCTTCCGGTCCTGGGGCCGGGTGGGCACAGTTATCGGCAGTAACAAACTTGAGCAGATGCCCTCCAA AGAGGACGCTGTTGAGCACTTCATGAAGCTGTATGAAGAGAAGACTGGGAATGCCTGGCACTCGAAAAAC TTCACAAAGTATCCCAAGAAGTTCTACCCTCTGGAGATTGACTATGGCCAGGACGAAGAGGCAGTAAAGA AGCTGACGGTGAAGCCTGGCACCAAGTCGAAGCTGCCGAAGCCAGTGCAGGAGCTCGTGGGGATGATCTT CGACGTGGAGAGCATGAAAAAGGCCTTGGTGGAGTACGAGATTGACCTCCAGAAGATGCCCTTGGGGAAG CTGAGCAGAAGGCAGATCCAGGCCGCCTACTCTATCCTCAGCGAGGTCCAGCAGGCAGTGTCTCAAGGCA GCAGTGAATCCCAGATCCTAGATCTCTCCAATCGCTTCTACACTCTGATCCCCCATGACTTTGGAATGAA GAAGCCCCCACTCCTGAACAACGCAGACAGCGTGCAGGCCAAGGTGGAGATGCTAGACAACCTCCTGGAC ATCGAGGTGGCCTATAGTCTTCTCAGGGGTGGCTCTGACGACAGCAGCAAGGATCCCATCGACGTCAACT ACGAGAAACTCAAAACTGACATTAAGGTGGTTGACAGAGATTCTGAAGAGGCCGAGGTCATCAGGAAGTA CGTGAAGAACACTCATGCTACCACGCACAACGCCTATGACCTGGAAGTGATCGATATCTTCAAGATAGAG CGCGAGGGGGAGAGCCAGCGCTACAAGCCCTTCAGGCAGCTTCACAACCGGAGGCTGCTGTGGCACGGCT CCAGGACCACCAACTTTGCTGGCATCCTGTCGCAGGGTCTGCGGATAGCCCCACCTGAAGCGCCTGTGAC AGGCTACATGTTTGGGAAAGGGATCTACTTTGCCGACATGGTGTCCAAAAGTGCAAACTACTGCCACACA TCTCAGGGAGACCCGATTGGCTTAATACTGCTGGGAGAGGTTGCCCTTGGAAACATGTATGAACTCAAGC ATGCTTCACATATCAGCAAGTTACCCAAGGGCAAGCACAGTGTCAAAGGTTTGGGAAAAACCACCCCTGA CCCTTCGGCCAGCATCACCCTGGAGGGTGTAGAGGTTCCACTGGGAACAGGGATCCCATCTGGTGTCAAC GACACCTGCCTGCTGTATAATGAGTACATTGTCTACGACATTGCTCAGGTGAATCTCAAATACCTGCTGA AACTCAAGTTCAATTTTAAGACATCCCTGTGG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG211449 representing NM_007415
Red=Cloning site Green=Tags(s) MAEASERLYRVEYAKSGRASCKKCSESIPKDSLRMAIMVQSPMFDGKVPHWYHFSCFWKVGHSIRQPDVE VDGFSELRWDDQQKVKKTAEAGGVAGKGQDGSGGKAEKTLGDFLAEYAKSNRSMCKGCLEKIEKGQMRLS KKMVDPEKPQLGMIDRWYHPTCFVKKRDELGFRPEYSASQLKGFSLLSAEDKEALKKQLPAIKNEGKRKG DEVDGTDEVAKKKSKKGKDKDSSKLEKALKAQNELIWNIKDELKKACSTNDLKELLIFNQQQVPSGESAI LDRVADGMAFGALLPCKECSGQLVFKSDAYYCTGDVTAWTKCMVKTQNPSRKEWVTPKEFREISYLKKLK VKKQDRIFPPESSAPAPLALPLSVTSAPTAVNSSAPADKPLSNMKILTLGKLSQNKDEAKAVIEKLGGKL TGSANKASLCISTKKEVEKMSKKMEEVKAANVRVVCEDFLQDVSASTKSLQELLSAHSLSSWGAEVKAEP GEVVAPKGKSAAPSKKSKGAVKEEGVNKSEKRMKLTLKGGAAVDPDSGLEHSAHVLEKGGKVFSATLGLV DIVKGTNSYYKLQLLEDDKESRYWIFRSWGRVGTVIGSNKLEQMPSKEDAVEHFMKLYEEKTGNAWHSKN FTKYPKKFYPLEIDYGQDEEAVKKLTVKPGTKSKLPKPVQELVGMIFDVESMKKALVEYEIDLQKMPLGK LSRRQIQAAYSILSEVQQAVSQGSSESQILDLSNRFYTLIPHDFGMKKPPLLNNADSVQAKVEMLDNLLD IEVAYSLLRGGSDDSSKDPIDVNYEKLKTDIKVVDRDSEEAEVIRKYVKNTHATTHNAYDLEVIDIFKIE REGESQRYKPFRQLHNRRLLWHGSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADMVSKSANYCHT SQGDPIGLILLGEVALGNMYELKHASHISKLPKGKHSVKGLGKTTPDPSASITLEGVEVPLGTGIPSGVN DTCLLYNEYIVYDIAQVNLKYLLKLKFNFKTSLW TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007415 |
| ORF Size | 3042 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007415.3 |
| RefSeq Size | 3845 bp |
| RefSeq ORF | 3045 bp |
| Locus ID | 11545 |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
NLRP3 mediates osteolysis through inflammation-dependent and -independent mechanisms
,Qu, C;Bonar, SL;Hickman-Brecks, CL;Abu-Amer, S;McGeough, MD;Peña, CA;Broderick, L;Yang, C;Grimston, SK;Kading, J;Abu-Amer, Y;Novack, DV;Hoffman, HM;Civitelli, R;Mbalaviele, G;,
FASEB J.
,PubMed ID 25477279
[PARP1]
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Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223198 | Parp1 (untagged) - Mouse poly (ADP-ribose) polymerase family, member 1 (Parp1), (10ug) |
CNY 10,600.00 |
|
| MR211449 | Parp1 (Myc-DDK-tagged) - Mouse poly (ADP-ribose) polymerase family, member 1 (Parp1) |
CNY 10,592.00 |
|
| MR211449L3 | Lenti ORF clone of Parp1 (Myc-DDK-tagged) - Mouse poly (ADP-ribose) polymerase family, member 1 (Parp1) |
CNY 11,970.00 |
|
| MR211449L4 | Lenti ORF clone of Parp1 (mGFP-tagged) - Mouse poly (ADP-ribose) polymerase family, member 1 (Parp1) |
CNY 11,970.00 |
