Iars2 (BC052403) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	MGC63429
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211440 representing BC052403 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACTGGGGGCTGTGCCCTCGCGGGCCCGGGGCGGCGGCGGTCGCCGCGGCTGGGAGCTTCTGGGGGC CAGCCCGTTTGCCCAGCCGCCTAGGGTGTCTGGGGATGACGAGGAGGCTCGTGGTACGATCCGTGGCCGG GGCGGACAGCCCCCAGAGCAGCTCGAAAGGCGGCAGATACCGTGACACGGTGCTGCTGCCGCAGACCAGC TTCCCGATGAAGCTGCTGGGCCGCCAGCAGTCCGACATGGAGCTGGAGATCCAGCAGAAATGTGGCTTTT CAGAGCTTTATTCCTGGCAAAGAGAAAGAAAAGTAAAGACGGAATTTTGCCTTCATGACGGGCCTCCTTA TGCCAACGGTGACCCTCATGTTGGGCATGCCCTGAACAAGATTTTGAAAGACATTGCCAATCGATTCCAT ATGATGAGAGGTTCCAAGGTACACTTTGTGCCTGGCTGGGATTGCCATGGCTTGCCCATTGAAACAAAAG TGTTATCAGAGCTCGGTGTAGATGCCCAGAGTCTTTCAGCCATGGAGATCAGGGAGAAAGCCAGATCATT TGCTCAGGCAGCCATTGAAAAGCAGAAATCGGCGTTTGTCCGCTGGGGAGTGATGGCAGACTGGAATAAC TGCTATTACACATTTGACCCAAAGTACGAAGCCAAGCAGCTGAGAGTATTCTACCAAATGTATGAGAAGG GCTTGGTTTATCGATCTTACAAACCAGTATATTGGTCTCCCTCATCAAGGACTGCGTTGGCTGAAGCAGA GCTTGAATACAACCCTGAGCATGTCAGTCGGTCGATATACGTAAGATTCCCCCTCCTGAGACCTCCTCCG AAGTTGGAGTCTCTTACAGATGCCTCATCCCCTGTTAGCGTTTTAGTTTGGACCACCCAGCCTTGGACAA TTCCCGCCAACCAGGCAATTTGCTATATGCCAGAGGCAAAGTATGCTGTGGTGAAATGTTCTGCCTCTGG GCACCTCTACATCCTGGCTGAAGATAAAATAGCACCTGTCGCTTCTGCCTTGGAAACAACATTTGATGTT GTTGCAGCATTTTCAGGTGTGGATCTGGAAGGTGGCACTTGTAGTCACCCTTTAACTCCCGATAAAGTGT CTCCTCTTTTACCTGCTACCCACGTGACTATGGCCAAAGGAACAGGGTTGGTTCACACAGCCCCAGCTCA CGGTATGGAAGATTACAGCGTAGCATCTCAGCACAGCCTCCCTATGGACTGTTTAGTGGATGAAGGCGGA ATGTTCACCGATGCCGCAGGCCCTGAACTTCAGAACAAGGCTGTACTTAAAGAAGGAACAGATGTGGTTA TAAAGATGCTTCAAGCTACCAAGAATGTGTTGAAAGAAGAGAACATTGTGCACAGCTATCCCTGTGACTG GAGAACTAAGACACCCGTGCTGATTCGAGCCAGCAAGCAGTGGTTTGTAAATATCACGGACATCAAGGCT GCAGCTAAGGAGTCGTTGAAGACGGTGAAGTTCATTCCCGGAGCCGCACTGAACAGCATGACGGACATGC TGGACAGACGGCCGTACTGGTGCATATCCCGGCAGAGAGTCTGGGGGGTTCCCATTCCTGTGTTTCATCA TAAGACAAAAGATGAATATTTGATCAACAGCCAAACCACTGAGCATATTATTAAACTCGTCGAGCAGCAC GGCAGTGATGTGTGGTGGACCCTCCCTGCTGAGCAGCTTCTCCCGGCAGAAGTCCTAGCTCAGGCTGGTG GCCCTGGGGCTTTGGAATACGCGCCAGGGCAGGACATTTTGGACATCTGGTTTGACAGTGGAACTTCATG GTCTTGTGTTCTTCAAGACACGCAGCAGAGGGCAGACTTGTACCTGGAGGGAAAGGACCAGCTCGGGGGC TGGTTCCAGTCTTCCTTACTAACAAGTGTGGCCACCAGGTCAAAAGCACCTTTCAGGACAGTGATGGTTC ATGGATTTACACTTGGAGAAAAAGGAGAAAAGATGTCCAAGTCCCTCGGGAATGTCATTAATCCTGACAC TATCATCAGTGGAGGAAAAGACCACAGCAAAGAACCTCCCTACGGAGCTGACATCCTGCGCTGGTGGATA GCTGAGTCCAATGTCTTCACGGAGGTGACCATTGGCCCCTCTGTACTCTCTGCTGCCAGAGATGACATCA GCAAGCTCAGAAACACACTCCGCTTTCTCTTGGGAAATTTGACCGGTTTTAACCCCGAGACAGATTCTGT TCCCGTGAAGAATATGTATGTCATAGACCAGTATATGCTGCACTTAATTCAGGATTTTGCGACAAAGATT ACTGACTCATACAAACAGTATGACTTTGGGAAAGTTGTCAGGCTGTTGAAAGCCTTCTACACCAGAGAAC TGTCCAGCTTTTACTTCAGCATAGTCAAGGATAGGCTCTACTGCGAAAACGAGAAGGATCCCAAGCGCCG CTCGTGTCAGACAGCATTGGCAGAAATTTTGGATGTGTTGGTCCGTGCTTTTGCCCCCATTCTTCCTCAC TTGGCAGAAGAGGTGTTTCAGCACATTCCCTATGTTACAGAACCCAAGAGTGTTTTTCGCACTGGCTGGA TTAATACAAGCTCTATTTGGAAGAAGCCAGGACTGGAGGAGGCAGTGGAAAGTGCGTGCGCCATGAGGGA CTCCTTTCTTGGCAGCATCCCTGGCAAAAATGCTGCTGAGTACGAGGTCATCATAGTGATCGAGCCAGGG CTGCTGTTTGAGATCATGGAGATGCTCCAAGCTGAGGAGACCTCTAGCACGTCTCAGCTGAATGAGCTGA TGATGGCTTCCCAGACGACATTGCTGGCTCAGGAGCCACGGGAAAGAACTGCAGGTGACATTGAGCTCAC GGGGACATTCGTCATCAATCTAGAAGGTGGTGACATTCGTGAGGAGTCTTCATATAAAGTCATTGTGGTA CCGACTGCCAGAGAGAAGTGCCCTCGATGTTGGAAGCATACCTCTGAGACTGCAGACGCTCTCTGTCCTC GGTGTGCAGAGGTCATTGGTGCAAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211440 representing BC052403 Red=Cloning site Green=Tags(s) MHWGLCPRGPGAAAVAAAGSFWGPARLPSRLGCLGMTRRLVVRSVAGADSPQSSSKGGRYRDTVLLPQTS FPMKLLGRQQSDMELEIQQKCGFSELYSWQRERKVKTEFCLHDGPPYANGDPHVGHALNKILKDIANRFH MMRGSKVHFVPGWDCHGLPIETKVLSELGVDAQSLSAMEIREKARSFAQAAIEKQKSAFVRWGVMADWNN CYYTFDPKYEAKQLRVFYQMYEKGLVYRSYKPVYWSPSSRTALAEAELEYNPEHVSRSIYVRFPLLRPPP KLESLTDASSPVSVLVWTTQPWTIPANQAICYMPEAKYAVVKCSASGHLYILAEDKIAPVASALETTFDV VAAFSGVDLEGGTCSHPLTPDKVSPLLPATHVTMAKGTGLVHTAPAHGMEDYSVASQHSLPMDCLVDEGG MFTDAAGPELQNKAVLKEGTDVVIKMLQATKNVLKEENIVHSYPCDWRTKTPVLIRASKQWFVNITDIKA AAKESLKTVKFIPGAALNSMTDMLDRRPYWCISRQRVWGVPIPVFHHKTKDEYLINSQTTEHIIKLVEQH GSDVWWTLPAEQLLPAEVLAQAGGPGALEYAPGQDILDIWFDSGTSWSCVLQDTQQRADLYLEGKDQLGG WFQSSLLTSVATRSKAPFRTVMVHGFTLGEKGEKMSKSLGNVINPDTIISGGKDHSKEPPYGADILRWWI AESNVFTEVTIGPSVLSAARDDISKLRNTLRFLLGNLTGFNPETDSVPVKNMYVIDQYMLHLIQDFATKI TDSYKQYDFGKVVRLLKAFYTRELSSFYFSIVKDRLYCENEKDPKRRSCQTALAEILDVLVRAFAPILPH LAEEVFQHIPYVTEPKSVFRTGWINTSSIWKKPGLEEAVESACAMRDSFLGSIPGKNAAEYEVIIVIEPG LLFEIMEMLQAEETSSTSQLNELMMASQTTLLAQEPRERTAGDIELTGTFVINLEGGDIREESSYKVIVV PTAREKCPRCWKHTSETADALCPRCAEVIGAK TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC052403
ORF Size	3036 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC052403, AAH52403
RefSeq Size	3499 bp
RefSeq ORF	3038 bp
Locus ID	381314

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