Atp2a3 (BC026147) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	SERCA3b, Serca3
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG211414 representing BC026147 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGAGGCGCACCTGCTCTCCGCTGCCGACGTGCTGCGTCGCTTCTCGGTGACAGCCGAGGGCGGCT TGAGCCTGGAGCAGGTGACCGACGCGCGGGAGCGTTACGGCCCCAACGAGCTCCCGACCGAGGAAGGGAA ATCCCTGTGGGAGCTGGTGGTGGAGCAGTTTGAGGACCTCTTGGTGCGCATCCTGCTGCTGGCAGCTCTG GTTTCCTTTGTCCTGGCCTGGTTCGAGGAGGGTGAGGAGACCACAACGGCCTTCGTGGAGCCCCTGGTCA TCATGCTAATTCTCGTGGCCAATGCGATCGTGGGAGTGTGGCAGGAACGCAACGCAGAGAGTGCAATTGA GGCCTTGAAGGAGTATGAGCCTGAGATGGGTAAGGTGATCCGGTCTGACCGCAAGGGTGTACAGAGGATC CGAGCCAGGGACATCGTCCCTGGGGACATTGTGGAAGTGGCAGTGGGAGACAAGGTACCCGCTGACCTCC GTCTCATCGAGATCAAGTCCACCACACTTCGAGTGGACCAGTCCATCCTGACAGGGGAATCCGTGTCTGT GACGAAGCACACAGATGCCATTCCAGACCCCCGAGCCGTGAACCAGGACAAGAAGAACATGCTATTTTCT GGCACCAATATTGCATCAGGCAAAGCCCTGGGTGTGGCTGTGGCTACAGGCCTGCAGACAGAGCTGGGCA AGATTCGGAGCCAGATGGCGGCTGTGGAGCCTGAGCGGACGCCATTGCAGCGCAAGCTGGATGAATTTGG GAGGCAGCTGTCCCATGCCATCTCTGTGATCTGTGTGGCTGTGTGGGTCATCAACATCGGCCACTTTGCT GACCCGGCCCACGGTGGATCCTGGCTCCGTGGTGCAGTCTACTACTTCAAGATCGCTGTGGCCCTGGCTG TGGCTGCTATCCCCGAGGGTCTCCCAGCAGTCATCACTACATGCCTGGCCCTGGGCACAAGACGTATGGC ACGTAAGAATGCCATTGTGCGGAGCCTGCCTTCTGTGGAGACGCTGGGCTGCACCTCGGTCATCTGCTCT GACAAGACGGGGACGCTCACCACCAATCAGATGTCTGTCTGCAGGATGTTCGTGGTGGCTGAAGCAGAGG CAGGCACCTGTCGTTTGCATGAATTCACCATTTCGGGTACCACGTACACCCCAGAGGGCGAAGTGAGGCA AGGGGAGCAGCCTGTGCGCTGTGGGCAGTTTGATGGGCTAGTGGAGCTCGCCACCATCTGTGCCCTCTGC AATGATTCTGCGCTGGACTACAATGAGGCCAAGGGTGTGTATGAGAAGGTAGGAGAGGCCACAGAGACCG CCCTGACTTGCCTGGTAGAGAAGATGAATGTGTTTGACACAGACCTGAAAGGACTGTCGCGGGTGGAGCG TGCTGGCGCCTGCAACTCAGTCATCAAGCAACTCATGCGGAAAGAGTTCACCCTGGAGTTCTCCCGGGAC CGGAAGTCTATGTCCGTGTACTGCACACCTACTCGTGCTGACCCCAAGGTCCAGGGCAGCAAGATGTTTG TGAAGGGAGCTCCTGAGAGTGTAATTGAGCGCTGTAGCTCAGTCCGAGTGGGCAGCCGAACAGCACCCCT GAGCACCACTTCCAGAGAGCATATCCTGGCCAAGATCCGGGACTGGGGCTCAGGCTCTGACACACTGCGC TGCTTAGCCTTGGCCACCAGGGACACACCCCCAAGGAAGGAGGACATGCATCTAGATGACTGCAGCCGGT TTGTACAGTACGAGACAGACCTGACCTTCGTGGGCTGCGTGGGCATGTTAGACCCACCGAGACCAGAGGT GGCTGCTTGTATCACACGCTGTTCCCGGGCTGGCATTCGAGTGGTCATGATCACAGGGGACAACAAAGGG ACAGCTGTGGCCATCTGCCGCCGACTTGGCATCTTTGGGGACACGGAGGATGTGTTGGGCAAGGCCTACA CGGGCCGTGAGTTTGATGACCTCAGCCCAGAGCAGCAGCGCCAGGCTTGTCGCACTGCCCGGTGCTTTGC CCGAGTGGAACCCGCACATAAGTCTCGAATCGTGGAGAACCTACAGTCCTTTAATGAGATCACTGCCATG ACTGGCGATGGGGTGAATGATGCACCAGCCCTGAAGAAGGCAGAGATCGGCATCGCCATGGGCTCAGGCA CTGCTGTGGCCAAGTCAGCAGCAGAGATGGTGCTGTCCGATGACAACTTTGCCTCCATTGTGGCTGCAGT AGAAGAGGGCAGGGCCATCTACAACAACATGAAGCAGTTCATCCGCTACCTCATCTCCTCCAATGTTGGC GAGGTTGTCTGCATCTTCCTCACAGCAATTCTGGGCCTGCCCGAAGCTCTGATCCCTGTGCAGCTGCTCT GGGTGAACCTGGTAACAGATGGCTTACCGGCCACAGCCCTTGGCTTCAACCCACCAGATCTGGATATCAT GGAGAAGCCGCCTCGGAATCCCCGTGAGGCCCTCATCAGTGGCTGGCTTTTTTTCCGCTATTTGGCTATT GGAGTGTATGTAGGCCTGGCTACAGTGGCTGCCGCCACCTGGTGGTTCCTGTATGACACCGAGGGACCTC AAGTCACCTTCTATCAGCTGAGGAACTTCCTGAAGTGCTCTGAAGACAATCCACTGTTTGCTGGCATTGA CTGCAAGGTTTTTGAGTCACGCTTCCCCACAACCATGGCCTTATCTGTGCTTGTGACCATTGAAATGTGC AACGCCCTCAACAGCGTCTCTGAGAACCAGTCACTGCTGCGCATGCCACCCTGGCTGAACCCTTGGCTGC TGGGGGCTGTGGTCATGTCCATGGCTCTGCATTTTCTTATCCTCCTGGTGCCACCTCTGCCTCTCATTTT TCAGGTGACCCCACTGAGTGGTCGGCAGTGGGGGGTGGTGCTTCAGATGTCTCTGCCTGTCATCCTGCTC GATGAGGCCCTCAAGTATCTGTCCAGGAATCACATGGATGAAAAAAAGGACCTGAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG211414 representing BC026147 Red=Cloning site Green=Tags(s) MEEAHLLSAADVLRRFSVTAEGGLSLEQVTDARERYGPNELPTEEGKSLWELVVEQFEDLLVRILLLAAL VSFVLAWFEEGEETTTAFVEPLVIMLILVANAIVGVWQERNAESAIEALKEYEPEMGKVIRSDRKGVQRI RARDIVPGDIVEVAVGDKVPADLRLIEIKSTTLRVDQSILTGESVSVTKHTDAIPDPRAVNQDKKNMLFS GTNIASGKALGVAVATGLQTELGKIRSQMAAVEPERTPLQRKLDEFGRQLSHAISVICVAVWVINIGHFA DPAHGGSWLRGAVYYFKIAVALAVAAIPEGLPAVITTCLALGTRRMARKNAIVRSLPSVETLGCTSVICS DKTGTLTTNQMSVCRMFVVAEAEAGTCRLHEFTISGTTYTPEGEVRQGEQPVRCGQFDGLVELATICALC NDSALDYNEAKGVYEKVGEATETALTCLVEKMNVFDTDLKGLSRVERAGACNSVIKQLMRKEFTLEFSRD RKSMSVYCTPTRADPKVQGSKMFVKGAPESVIERCSSVRVGSRTAPLSTTSREHILAKIRDWGSGSDTLR CLALATRDTPPRKEDMHLDDCSRFVQYETDLTFVGCVGMLDPPRPEVAACITRCSRAGIRVVMITGDNKG TAVAICRRLGIFGDTEDVLGKAYTGREFDDLSPEQQRQACRTARCFARVEPAHKSRIVENLQSFNEITAM TGDGVNDAPALKKAEIGIAMGSGTAVAKSAAEMVLSDDNFASIVAAVEEGRAIYNNMKQFIRYLISSNVG EVVCIFLTAILGLPEALIPVQLLWVNLVTDGLPATALGFNPPDLDIMEKPPRNPREALISGWLFFRYLAI GVYVGLATVAAATWWFLYDTEGPQVTFYQLRNFLKCSEDNPLFAGIDCKVFESRFPTTMALSVLVTIEMC NALNSVSENQSLLRMPPWLNPWLLGAVVMSMALHFLILLVPPLPLIFQVTPLSGRQWGVVLQMSLPVILL DEALKYLSRNHMDEKKDLK TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC026147
ORF Size	2999 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	BC026147, AAH26147
RefSeq Size	4489 bp
RefSeq ORF	2999 bp
Locus ID	53313
Gene Summary	This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.[UniProtKB/Swiss-Prot Function]

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